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Tissue Culture Technique (Kappaphycus Alvarezii) Using a Somatic Embryogenesis Approach seen from Management and Implementation Hardianto, Toto; Mustafa; Suleman, Yakub; Nurul Awaliah; Feby Triadi
Jurnal Ad'ministrare: Jurnal Pemikirian Ilmiah dan Pendidikan Administrasi Perkantoran Volume 11, Issue 1, 2024
Publisher : Pendidikan Administrasi Perkantoran, Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71309/administrare.v11i1.1838

Abstract

Seaweed is a superior commodity for aquaculture and has the largest production among other superior commodities. The factor that influences the success of seaweed cultivation is the selection of seeds, using good quality seeds because it can increase seaweed production. Therefore, seaweed produced through tissue culture techniques has the advantages and disadvantages of being able to be cultivated in turbid waters, being able to survive at low salinity and being resistant to high rainfall. Meanwhile, to make it more focused, an administrative concept is needed in it regarding management and implementation, so you will see in this journal a very clear administrative approach. The results of the research showed that the stages of the seaweed tissue culture technique started from broodstock acclimatization, sterilization test of the esplank thallus, callus induction, regeneration of embryogenic callus into micropropagules, regeneration of micropropagules into propagules (Plantlets), and maintenance of plantlets. The problem that usually occurs in seaweed tissue culture is human error. This can create sterility during the culture process, so that microorganisms can enter and contaminate the explants. In addition, the entry of microorganisms can be caused by the hole where the hose enters being too wide so that air from outside which may contain microorganisms can enter. The holes created can also cause evaporation or evaporation of sea water so that the salinity rate increases. Another situation that occurs is a lack of air supply from aeration because the internal capacity of the blower which supplies air is weak and uneven. This can cause the explants to accumulate and stick to the walls of the bottle so that nutrient absorption is hampered
Pertumbuhan dan Rasio Konversi Pakan Udang Vaname yang Dipelihara pada Sistem Bioflok Menggunakan Ampas Tebu (Saccharum officinarum Linn.) Sebagai Sumber Karbon Anton, Anton; Alauddin, Muhammad Hery Riyadi; Yunarty, Yunarty; Renitasari, Diana Putri; Hardianto, Toto; Kurniaji, Ardana
Jurnal Salamata Vol 6, No 2 (2024): Desember
Publisher : Politeknik Kelautan dan Perikanan Bone

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/salamata.v6i2.14071

Abstract

Udang vaname (Litopenaeus vannamei) adalah salah satu komoditas budidaya perikanan yang bernilai ekonomistinggi. Pengembangan budidaya udang vaname saat ini mengarah pada intensifikasi dengan sistem bioflok.Penelitian ini bertujuan untuk menganalisis pengaruh penggunaan ampas tebu sebagai sumber karbon terhadappertumbuhan dan rasio konversi pakan (RKP) udang vaname. Penelitian menggunakan tiga perlakuan dan tigaulangan yakni aplikasi ampas tebu sebagai sumber karbon bioflok (AmpT), aplikasi tepung terigu sebagaisumber karbon bioflok (TpgT) dan kontrol tanpa bioflok (Kontrol). Ampas tebu diperoleh dari perusahaan pabrikgula di Kabupaten Bone. Tepung ampas tebu dipreparasi dengan oven 60oC dan digiling dengan mesin penepung.Udang vaname berukuran 18 g/ekor diperoleh dari Tambak Politeknik Kelautan dan Perikanan Bone. Pemeliharaandengan sistem bioflok dilakukan selama 30 hari. Hasil penelitian menunjukkan bahwa aplikasi ampas tebusebagai sumber karbon dapat meningkatkan laju pertumbuhan spesifik (LPS) sebesar 0,57±0,01% (AmpT)dibandingkan kontrol 0,33±0,14%. LPS tertinggi diamati pada perlakuan tepung terigu yakni 1,39±0,51% (TpgT).Aplikasi ampas tebu juga dapat menghasilkan RKP lebih rendah yakni 1,36±0,06 (AmpT) dibandingkan kontrol1,69±0,08. Penelitian ini menunjukkan potensi ampas tebu digunakan sebagai sumber karbon pada budidayaudang vaname berbasis sistem bioflok.
Aplikasi Pemberian Dosis Pupuk Provasoli’s Enriched Seawater (PES) Yang Berbeda Pada Produksi Bibit Gracillaria verrucosa Melalui Kultur Jaringan Dengan Metode Propagasi Vegetatif Rasnijal, Muhammad; Alauddin, Muhammad Hery Riyadi; Budiyati; Anton; Muhammad Syahrir; Yunarty; Saridu, Siti Aisyah; Wahid, Eriyanti; Regan, Yip; Hardianto, Toto; Supryady; Ihwan; Ernawati; Anwar; Mulato, Alwi; Sucipto; Syarief, Muhammad Nurman; Suleman, Yakub; Andini, Salsa; Suleman, Gabriella Augustine; Mulyono, Mugi; Anggoro, Agung Doni; Sektiana, Sinar Pagi; Achmad Suhermanto
JARI : Jurnal Akuakultur Rawa Indonesia Vol. 13 No. 2 (2025): JARI: JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Program Studi Budidaya Perairan, Fakultas Pertanian, Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/jari.v13i2.71

Abstract

Cultivation of the seaweed Gracilaria verrucosa is necessary as a preventive measure against overexploitation. One applicable method is tissue culture, which requires special attention to nutrient needs to support accelerated growth. These nutrients can be provided through fertilization. One commonly used chemical fertilizer in seaweed tissue culture is Provasoli’s Enriched Seawater (PES). This study aims to determine the optimal PES fertilizer dosage for the production of G. verrucosa seedlings through tissue culture using a vegetative propagation method. This study used a completely randomized design with analysis of variance (ANOVA) consisting of four treatments of different PES fertilizer doses with three replicates for each treatment. The results showed that fertilizer dose variation did not significantly affect the absolute growth of explant weight, but tended to increase the number of growth points. Growth points began to increase in the second week for all treatments, and by the fourth week, the number of growth points at a 1.5% dosage showed a significant difference compared to other doses. The application of PES fertilizer in G. verrucosa seedling production through tissue culture with vegetative propagation indicates that different fertilizer doses do not affect explant growth but significantly influence the increase in growth points by the fourth week