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BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan)

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Published by Universitas Medan Area

ISSN : 25975269 EISSN : 2356458X DOI : 10.31289

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Biolink focuses on the publication of the results of scientific research related to fields. This article is published in the internal and external academic community of the University of Medan Area (UMA) especially in the field of Biology. Biolink publishes scientific articles in the scope of biology that includes environmental biology, industrial biology and health biology. Published articles are the results of research articles, studies or critical and comprehensive scientific studies on important issues related to the field of biology.

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Articles 378 Documents

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IDENTIFICATION AND INVENTORY OF CASSAVA (Manihot esculenta Crantz) IN NORTH SUMATERA Emmy Harso Kardhinata; Edison Purba; Dwi Suryanto; Herla Rusmarilin
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 1 (2020): August 2020
Publisher : Universitas Medan Area

The study of identification and inventory of cassava accessions was done from August to September 2014 in four districts based on their potential as a center for cassava cultivation, namely Simalungun, Serdang Bedagai, Deli Serdang, Langkat Regency representing the lowlands and Simalungun and Karo Regency representing the highlands. Each district was selected 3 subdistricts and each subdistrict was surveyed 3 villages randomly through the accidental sampling method. Guidance on identifying morphological characters was used by reference from Fukuda, et al. (2010) by giving a score for each character observed. The results of the study obtained 8 genotypes with their respective codes and local names; 1) Sawit (G1), 2) Lampung (G2), 3) Merah (G3), 4) Adira-1 (G4), 5) Kalimantan (G5), 6) Malaysia (G6), 7) Roti (G7) and 8) Klanting (G8). The most common genotype found in the location were Malaysia and Adira-1, while the rarest was Merah. The yield potential at the age of 10 months were Sawit (26,9–30,1 tons/ha), Lampung (29,6–52,6 tons/ha), Merah (54–61,6tons/ha), Adira-1 (34,8–55,0 tons/ha), Kalimantan (15,8–64,2 tons/ha), Malaysia (32,9–64,3 tons/ha), , Roti (24,7–55,5 tons/ha), and Klanting (327,4–49,8 tons/ha).

MAPPING OF CONSERVATION AREA PLAN IN NORTH POLOMBANGKENG DISTRICT, TAKALAR REGENCY Nurul Fajeriana; Muhammad Arifin Abd Kadir
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 1 (2020): August 2020
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v7i1.3496

North Polombangkeng District is one of the districts in Takalar Regency, South Sulawesi, where 44% of the total land area of 9495 ha out of 21225 ha are steeply sloped areas. Therefore we need a policy direction on the arrangement of the use of conservation areas to support the sustainability of the existing environment in the form of mapping conservation area plans. This study uses a soil survey method and evaluation of land capability. Physical Factors the environment is a limiting factor in determining land use policy, namely slope factors, soil sensitivity to erosion, and rainfall intensity. The research shows that Barugayya Village, Towata Village, and Kampung Baru Village are included in class IV land grade classification and Ko'mara Village in Class VI. At the same time, the Scoring Assessment of Physical Environmental Factors in Forest Areas has a range of values ranging from 160 to 215, the area of which is planned to be a conservation area. Determination of Conservation Area refers to the function criteria of forest area based on the scoring System of the Minister of Agriculture Regulation in Minister of Agriculture Decree No. 837/Kits/Um/11/80.

REDUCING SUGAR CONTENT IN SIWALAN ROOMIE (Borassus flabellifer L) BEFORE COOKING AND LIQUID BROWN SUGAR AFTER COOKING James Ngginak; Sonya Titin Nge; Filorita F. Klau; Coni Lisandra Balle Bisilissin
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 1 (2020): August 2020
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v7i1.2919

Sugar reduction is one of the essential macronutrients used as energy sources. High levels of sugar reduction in food can increase the sweet taste of these foodstuffs. However, the consumption of high sugar food can cause diabetes mellitus. The purpose of this study is to increase the economic value of liquid palm sugar (Borassus flabellifer L) and opportunities as a preservative in the food industry. This research used quantitative descriptive methods. The results showed that the level of sugar reduction in siwalan liquid brown sugar (Borassus flabellifer L) was 15.65%, the water content in the liquid palm sugar sample (Borassus flabellifer L) is 26.78%, and the temperature of the liquid brown sugar after cooking is 230C. The effect of heat or temperature on cooking also causes physical changes in liquid brown sugar such as caramel formation, color and flavour.

DIURETICS EFFECT OF AVOCADO LEAF (Persea americana Mill.) ETHANOL EXTRACT ON THE GASTRIC HISTOLOGY OF WISTAR MALE RATS (Rattus norvegicus) Dwi Erlinda; Mahriani Mahriani; Susantin Fajariyah
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 1 (2020): August 2020
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v7i1.3007

Irregular urine output triggers kidney stone disease. One way to cure it is by using synthetic diuretic compounds. The use of synthetic diuretic drugs for a long time can cause side effects, namely gastric damage. Therefore, research is needed to determine the diuretic effect of ethanol extract of avocado leaves (Persea americana) and its effect on the histological structure of the gastric of male rats. Avocado leaf extract is made by maceration method, using 70% ethanol and then evaporated. Wistar male rats were divided into 5 treatment groups namely negative control (aquadest), positive control (furosemide), avocado leaf extract dose of 50 mg / kg body weight, 100 mg / kg body weight, and 150 mg / kg body weight with oral administration for 15 days. The volume of urine was measured for 24 hours, then the mice were sacrified on the 16th day. Gastric organs were taken for histological observation by the paraffin method and Hematoxylin and Eosin (H&E) staining. The results showed that the optimum avocado leaf ethanol extract was a dose of 100 mg/kg showed a diuretic effect but did not affect the histological structure of the gastric.

ECOTOURISM DEVELOPMENT OF MUNJANG MANGROVE FOREST AND CONSERVATION EFFORTS BASED ON COMMUNITY APPROACH Henri Henri; Seta Ardiawati
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 1 (2020): August 2020
Publisher : Universitas Medan Area

Ecotourism development in a mangrove forest area includes many positive and negative aspects and requires a sustainablecomprehensive study. Ecotourism potential must be assessed to provide understanding of local people in managing natural resources. This study aims at identifying the potential for the development of ecotourism and determining the efforts of conservation strategies for supporting mangrove forests. Data collection was done by questionnaires and interviews using 1-5 Likert scale measurement and SWOT analysis to determine ecotourism development strategies. The results of the study indicates that Munjang mangrove forest has a very good potential to be developed as an ecotourism area. It is supported by the efforts of conservation as the main key in realizing the development of sustainable ecotourism. The role of the community and stakeholders has been well established such as mangrove seeding and nursery activities. Finally, this study recognizes that the capacity of sustainability indicators is very important to map the transformation and constant challenges of supporting mangrove ecotourism, so that it can provide conservation benefits, provide quality services for visitors and improve local communities welfare.

THE DNA SEQUENCE ENCODING GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE (GAPC) ENZYME ON TUNTUN ANGIN PLANT (ELAEOCARPUS FLORIBUNDUS BI) Dewi Indriyani Roslim; Hestia Hairima; Herman Herman; Wahyu Lestari
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 1 (2020): August 2020
Publisher : Universitas Medan Area

Glyceraldehyde 3-phosphate dehydrogenase (GapC) is an enzyme involved in glycolysis. The expression of this gene tends to abundant in eukaryotic cells, so this gene is frequently used as an internal control in gene expression analysis. This research aims to isolate the DNA sequence of the GapC gene from tuntun angin (Elaeocarpus floribundus BI). Methods included a collection of the leaves from Kajuik Lake, Riau Province then the DNA extraction, electrophoresis, amplification of partial DNA sequence of GapC gene, cloning and sequencing. The DNA sequence was analyzed using the BLASTn program and MEGA6 software. The GapC sequence obtained in this study was 933 bp in size, consisting of four introns and five exons, and encoding 137 deduced amino acids. The BLASTn analysis showed that the sequence has 89.84%-90.16% similarity to other species of Cunoniaceae family, such as species from the genus of Spiraeanthemum and Codia. The parsial sequence of E. floribundus GapC gene was more resemble the one of Spiraeanthemum than Codia genus. The GapC sequence obtained in this study was the first reported from the Elaeocarpaceae family. This sequence has the opportunity to serve as an internal control after validation.

DIVERSITY OF FERNS (PTERIDOPHYTA) IN THE SEVERAL MOUNTAINS OF WEST JAVA Suryana Suryana; Betty Mayawatie; Joko Kusmoro; Budi Irawan
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 1 (2020): August 2020
Publisher : Universitas Medan Area

The diversity of ferns (Pteridophyta) in the five mountainous in West Java (Mt. of Patuha, Papandayan, Tangkuban Perahu, Pangrango, and Guntur) has not been widely reported. The aim of this research was to obtain data on diversity and similarity of fern species in the five mountains in West Java. This research was conducted by exploring and descriptive analysis method. Comparison of species diversity among all reseach field were calculated by the index of species similarity according to Sorenson. The diversity of ferns in Mt. Patuha, Papandayan, Tangkuban Perahu, Pangrango, and Guntur were 27, 14, 26, 40 and 5 species, respectively. The total number of ferns species in five locations are 83 species from 25 families. Comparison of species diversity among fileds based on the Sorenson similarity index is low (<50%), except between the Mt. Patuha and Mt. Papandayan areas is high (> 50%).

IDENTIFICATION OF SECONDARY METABOLIC AND TEST OF ACTIVITY ETHYL ACETATE FRACTION OF BANGUN¬- BANGUN (Coleus amboinicus Lour.) LEAVES AS ANTIOXIDANT Kasta Gurning
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 1 (2020): August 2020
Publisher : Universitas Medan Area

Antioxidants have an important role in delaying the oxidation process and preventing the occurrence of degenerative diseases in the body. Antioxidants consist of synthetic and natural; the use of synthetic antioxidants in a relatively long period is hazardous so that the use of natural antioxidants is considered more potential and safe for the body. The process of extracting secondary metabolites from the leaves of bangun-bangun (Coleus amboinicus L.) is carried out by the maceration method with methanol, then partitioned in stages with n-hexane, chloroform, and ethyl acetate. This research intends to identify secondary metabolites and test antioxidant activity from the ethyl acetate fraction with the DPPH method. Phytochemical screening results of ethyl acetate fraction showed the different secondary metabolite groups such as alkaloids, phenolics & polyphenols, flavonoids, coumarin, and triterpenoids. Antioxidant activity (IC50) obtained is 64.97 with a healthy category, so that the potential to be used as a natural antioxidant.

THE FLAVONOID AND ALKALOID CONTENT OF CYCLOSORUS PARASITICUS (LINN.) FARWELL FERNS AT THE PLANTATION AREAS OF JEMBER REGENCY Dwi Setyati; Hari Sulistiyowati; Monica Paulina Erizcy; Tri Ratnasari
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 1 (2020): August 2020
Publisher : Universitas Medan Area

Cyclosorusparasiticus(Linn.) Farwell is one potential medicinal ferns. The plant contains secondary metabolites, such as flavonoids and alkaloids. This study aims to determine the flavonoid and alkanoidofCyclosorusparasiticus (Linn.) Farwell in three plantation areas of Jember Regency. Plant samples were collected from Mount Gumitir coffee plantations, Pine Garahan Village and rubber and cocoa plantations in Tancak in Jember. Samples of stems and leaves were dried at room temperature and then blended to obtain a powder. One gram of powder samples was macerated in 90 ml of methanol for 3x24 hours then was concentrated with an evaporator to obtain a crude extract. The crude extract was tested qualitatively for flavonoids and alkaloids by the Willstätter and Dragendorff methods followed by quantitatively tests with Spectrophotometric. The results showed thatboth flavonoids and alkaloids were found in stem and leavesof Cyclosorusparasiticus (Linn) Farwell growing at three research locations. The flavonoids and alkaloidsin content found in leaveswas higher than those in stem organs. The leaves of Cyclosorusparasiticus (Linn) Farwell in Gumitir contain the highest flavonoids and alkaloidscontentsthan those in Tancak.

EFFECTIVENESS TEST OF ANTI-BACTERIAL LIME JUICE (Citrus aurantifolia) ON SALMONELLA SP BACTERIA IN SALMON FISH (Oncorhynchus nerka) Qori Fadillah; Linda Chiuman; Chrismis Novalinda Ginting; Ermi Girsang
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 1 (2020): August 2020
Publisher : Universitas Medan Area

Salmonella Sp is a gram-negative pathogens that are often found in marine foods,one of process of serving salmon is channeling lime juice, where lime juice is acidic and has phytochemical properties that have anti-bacterial properties. This study aims to explore anti-bacterial effect lime juice on Salmonella thypii bacteria in Salmon. This research is an experimental study using disc diffusion method. Lime samples that were used were purchased from one of the modern markets in Medan which were made in several series of concentrations using distilled water as a solvent, while the salmon samples used in this study were purchased from one of the Japanese dining restaurants located in Medan City. Meanwhile, isolation of Salmonella thypii bacteria from salmon samples was carried out using nutrient broth and SSA media. The results of this study indicate that differences in concentration indicate the differences in the diameter of the inhibitory zone (P value = 0.000). With the diameter of the widest inhibition zone formed by a concentration of 100% is 15.40 ± 0.44 mm and the smallest diameter is formed by a concentration of 5% which is 7.23 ± 0.55 mm. Thus, it can be concluded that the lime juice has anti-bacterial activity against Salmonella thypii bacteria from salmon.

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