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INDONESIA
Jurnal Biodjati
Published by Universitas Islam Negeri Sunan Gunung Djati Bandung
ISSN : 25481606     EISSN : 25414208     DOI : 10.15575/biodjati
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences
Arjuna Subject : -
Articles 482 Documents
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Comparison of Quality and Ethanol Content of Liquid and Powder Kombucha from Jasmine Tea (Jasminum sambac L.) Nisa Ihsani; Anisa Nurul Huda; Arifa Nadya Nur Afifah; Tiwi Sartika; Linda Pertiwi; Maelita Ramdani Moeis; Muhammad Farid Maksum; Aila Gema Safitri; Oktira Roka Aji
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.47357

Abstract

The fermentation process of kombucha has become a focus of halal product research due to its ethanol content. Processing kombucha into powder form can be an alternative to address the ethanol content in liquid kombucha, as it has undergone a drying process, making it a potential halal-certified functional drink. The objective of this study was to compare the quality of liquid and powdered kombucha. The research was conducted from March to September 2024 at the Biotechnology Laboratory, Muhammadiyah University of Bandung. The method used was the Complete Randomized Design (CRD) method. The parameters analyzed included ethanol content, total BAA (Bacterial Acetic Acid), pH, organic acid content, and sugar content. In the production of powdered kombucha, optimization of maltodextrin and gum arabic usage was also conducted to achieve the best solubility and taste. The best powdered kombucha was obtained with a maltodextrin-to-gum arabic ratio of 3:2. In the research, liquid kombucha contained ethanol at an average of 0.09%. In comparison, no ethanol was detected in the powdered kombucha (0%). Total BAA in powder decreased after the drying process (p<0.05). The pH value of kombucha in liquid form (4.4) also differed significantly from powdered (3.2) (p<0.05). However, the levels of organic acids and sugars in liquid and powdered kombucha did not differ significantly (p>0.05). The sugar content in liquid and powdered kombucha was lower than that in kombucha in general.
Survival Responses of Two Termite Genera to Environmental Stressors as Bioindicators of Climate Change Kuswanto, Eko; Pratama, Anisa Oktina Sari; Moh. Dwi Kurniawan Hasan; Aksel Fadly Masamanda; Zikri Al Rosyid; Ferry Andreansyah; Nico Okta Arniansyah
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.48347

Abstract

Termites are sensitive to environmental fluctuations and hold potential as bioindicators of climate change. This study evaluated the survivability of Nasutitermes and Macrotermes under controlled variations in temperature, relative humidity (RH), and CO₂ concentration. Laboratory experiments were conducted using eleven temperature levels (0–50°C), seven RH levels (40–100%), and four CO₂ concentrations (500–2000 ppm). Each treatment was replicated three times with 50 worker termites per replicate. Survivability, measured as percent survival after one hour of exposure, was analyzed by one-way ANOVA followed by Tukey’s HSD test (p < 0.05). The results indicated that both genera exhibited sharp declines in survival under temperature extremes and elevated CO₂. Optimal survivability for Nasutitermes and Macrotermes occurred at moderate temperatures (25–35°C), relative humidity (60–80%), and ambient CO₂ levels (500 ppm), while extreme conditions significantly increased mortality. The study highlights species-specific tolerance thresholds and confirms that environmental stressors directly affect termite physiology and behavior. These results confirm termites’ potential as reliable bioindicators, providing a practical tool for monitoring ecosystem responses to climate stress and informing strategies for sustainable ecosystem management.
Bioprospecting of Potential Microorganisms as Agents for Biodiesel Production from Sago Pulp Waste Feedstock Lethulur, Alice Chaela; Seumahu, Cecilia Anna; Apituley, Edwin Thomas
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.48431

Abstract

The rising global energy demand and environmental issues associated with fossil fuel use have accelerated interest in renewable alternatives, such as biodiesel. This study aimed to provide a preliminary screening of potential indigenous microorganisms among isolates with high ability to saccharify lignocellulosic sago pulp waste, an abundant, non-food biomass in Maluku, and to accumulate lipids. Laboratory experiments were conducted to isolate, characterize (macro- and microscopically), saccharify, and accumulate lipids. Bacterial isolates were evaluated for saccharification ability using two parameters: the cellulolytic index (CI), determined by the clear zone on solid CMC media, and reducing sugar production in modified liquid CMC media using pretreated sago waste, measured by Benedict’s test. Yeast and mold isolates were assessed for lipid accumulation through Sudan Black B staining and gravimetric lipid extraction under nitrogen-limited conditions. Results identified four bacterial isolates capable of saccharification, with isolate BAS 1B showing the highest cellulolytic index, and all isolates produced detectable reducing sugars by Benedict’s test. All yeast and mold isolates accumulated intracellular lipids, with YAS 2 and KAS 2 isolates accumulating the highest lipid compared to the positive control (Saccharomyces cerevisiae). As this study was based on a single-point observation (n=1), the results are exploratory and should be interpreted cautiously. Nevertheless, isolates BAS 1B, YAS 2, and KAS 2 show promising potential for further biodiesel-related research using lignocellulosic waste. Further studies with replication and quantitative validation are required prior to any industrial consideration
Assessing the Ecological Status of the Curug Tilu Leuwi Opat Waterfall Using Macroinvertebrates and Physicochemical Parameters Hanif Syafrian Purnama; Tati Suryati Syamsudin; Dian Rosleine
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.49080

Abstract

Anthropogenic activities increasingly threaten freshwater availability. The Curug Tilu Leuwi Opat waterfall in the Cimahi River, an important water source for surrounding communities and a popular tourist site, requires an ecological assessment to support effective water management. This study aims to provide an initial overview of the ecological status of waters through an integrative approach that combines benthic macroinvertebrates and physicochemical parameters. Sampling was conducted in two waterfall streams, Kacapi and Aseupan, which are part of the Curug Tilu Leuwi Opat waterfall system. Physicochemical parameters measured included temperature, flow velocity, pH, dissolved oxygen, conductivity, and total dissolved solids. Benthic macroinvertebrates were collected using a Surber net and identified to the lowest feasible taxonomic level, typically morphospecies with some taxa at the family level, and subsequently analyzed using the Shannon–Wiener index, Simpson’s dominance, the Family Biotic Index (FBI), and Canonical Correspondence Analysis. Results indicated excellent water quality (FBI at Kacapi = 4.17; Aseupan = 4.24), driven by the presence of pollution-sensitive taxa from Leptophlebiidae, Tipulidae, and Perlidae, while the slightly higher FBI at Aseupan reflected several moderately tolerant families such as Physidae and Simuliidae. Benthic macroinvertebrate diversity was moderate with no dominant species, indicating stable community structure. Most measured parameters that met established standards met Class I criteria, while dissolved oxygen, conductivity, and flow velocity emerged as the dominant variables shaping community structure. Overall, the aquatic ecological status was excellent, highlighting the role of aquatic biodiversity as a scientific basis for sustainable environmental management
The Effect of IAA, BAP, and Coconut Water on Tomato (Solanum lycopersicum L.) Organogenesis Alba Wijaya Maharani; Restiani, Ratih; Aniek Prasetyaningsih
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.49410

Abstract

Tomato (Solanum lycopersicum L.) is a widely cultivated horticultural crop valued for its phytochemical and nutritional content. The increasing demand for high-quality seedlings has promoted the use of in vitro propagation, which offers greater efficiency in labor and land use, uniform seedling production, and independence from climate compared to conventional methods. This study aimed to investigate the effect of different concentrations of plant growth regulators (PGRs) and coconut water as an alternative or supplement on the organogenesis of tomato hypocotyl explants. Tomato hypocotyl explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of BAP (0.5, 1.0, and 1.5 ppm), IAA (0.1, 0.3, and 0.5 ppm), and coconut water (10%, 20%, and 30%). The experiment was arranged in a Completely Randomized Design (CRD) with four replications, consisting of a control, single-factor treatments, and combination treatments of BAP, IAA, and coconut water. Observations included the percentage and intensity of root, shoot, and callus formation, as well as shoot height. Data were analyzed using the non-parametric Kruskal–Wallis test, followed by Dunn’s test. The results showed that roots, shoots, and callus successfully developed in treatments supplemented with 6-benzylaminopurine (BAP), indole-3-acetic acid (IAA), and coconut water. The highest shoot growth, with a percentage of 87.7% and an average height of 5.75 cm, was observed in treatment K20, while the highest callus formation occurred in treatment K30. The best shoot and callus intensities were resulted in media with coconut water alone at concentrations of 10%, 20%, and 30%, whereas the highest root intensity was obtained in the BAP+IAA treatment without coconut water. The results indicate that coconut water is effective in supporting organogenesis, both directly through the formation of shoots and roots, and indirectly through callus formation that can subsequently develop into shoots and roots.
Etnobotanical Study of Medicinal Plants by the Community Pucung Village, Kismantoro District, Wonogiri Regency, Central Java Nur Kholis Majid, Muhammad; Agustina, Ana; Lasekti Wicaksono, Rezky
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.49761

Abstract

Indonesia is known as a living laboratory because its forests host approximately 30,000 plant species, of which around 9,600 are medicinal. The benefits of natural medicines derived from plants have been empirically proven, encouraging communities to preserve them. The use of medicinal plants by communities reflects the relationship between humans and plants, known as ethnobotany. This relationship can show how the community of Pucung Village uses medicinal plants, the efforts and conservation status of the medicinal plant species found, and the community's perceptions of medicinal plants and the sustainability of local knowledge about their use for future generations. Using an ethnobotanical approach, this study focuses on local wisdom as the foundation for preserving knowledge of medicinal plants in Pucung Village. The data collection methods used were purposive sampling and snowball sampling. Using these techniques, 18 informants were identified. Based on the interview results, 56 plant types from 30 families were identified, with turmeric (100%) being the most frequently used. The plant part with the highest percentage was leaves (45.16%). The most dominant processing method was boiling (37.70%). The primary source of plant acquisition was cultivation activities (49.30%). The most common plant habit was herbaceous (37%). There are 24 medicinal plant species classified as least concern, 9 as data deficient, and 23 as not evaluated. Efforts to sustain local knowledge include direct practice and the transmission of knowledge through oral communication. Nine informants tend to use medicinal plants to maintain physical health, while nine others tend to prefer using medicinal plants first.
Detection of Porcine Contamination in Beef Meatballs from Gedangan District Using PCR–RFLP Suryadi, Gian Firdaus Putra; Astuti, Rahma Windi; Fitriani, Lilis Nur; Lisdiana, Lisa
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.49813

Abstract

Meatball is one of the popular meat dishes in Indonesia, especially in Sidoarjo. The sale of meatballs in Sidoarjo is carried out by Micro, Small, and Medium Enterprises (MSMEs), with 330 of the total 11,114 micro-traders selling meatballs. However, there is a risk of pork being adulterated into meatballs. This is a serious problem because Indonesia, especially Sidoarjo, is a Muslim-majority city with 95.5% of the total population of 2 million. Therefore, the purpose of this article is to conduct field checks to determine whether or not pork contamination is present in meatballs in Sidoarjo. Sampling locations were determined using a stratified random sampling method, which found that eight of the 15 villages, including Karangbong, Tebel, Sruni, Keboananom, Keboansikep, Gedangan, Ketajen, and Sawotratap Villages, have a significance value greater than the alpha value (0.05). Therefore, eight of them were used as sampling locations. One sample was purchased from each of the eight chosen villages, named S1, S2, S3, S4, S5, S6, S7, and S8. Meanwhile, the possibility of porcine contamination in beef meatball samples was analyzed using the Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) method with the restriction enzyme BsaJI. This study found that all eight samples and the negative control DNA amplicons were not cleaved by the BsaJI enzyme. Meanwhile, the positive control DNA amplicon was cleaved into 3 fragments. It was indicated that all samples and the negative control DNA amplicons were free of pork DNA contamination. Despite the anomaly, it can be concluded that all meatball samples bought from Gedangan District are not contaminated with pork. By demonstrating that meatballs from Sidoarjo are free from pork DNA, this study serves as an additional halal food monitoring in Sidoarjo. It reinforces public trust in local MSME food products.
Studi Anatomi Stomata Daun Mangga (Mangifera indica) Berdasarkan Perbedaan Lingkungan Mutaqin, Asep Zainal; Budiono, Ruly; Setiawati, Tia; Nurzaman, Moahamad; Fauzia, Radewi Safira
Jurnal Biodjati Vol 1 No 1 (2016): Jurnal Biodjati, Volume I, Nomor 1, November 2016
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v1i1.1009

Abstract

Abstrak. Penelitian ini bertujuan untuk mengetahui stomata daun mangga (Mangifera indica) di daerah dengan kondisi lingkungan yang berbeda. Penelitian bersifat deskriptif analisis. Pengambilan sampel dilakukan melalui survey secara acak di Cagar Alam Pananjung Pangandaran dan salah satu ruas jalan di Kecamatan Pangandaran Kabupaten Pangandaran. Paramater yang diamati adalah kerapatan dan kerusakan stomata. Selain itu diukur juga luas daun, berat partikel debu yang menempel di daun, serta beberapa faktor lingkungan fisik seperti intensitas cahaya, suhu, dan kelembaban udara. Hasil penelitian menunjukkan bahwa kerapatan dan kerusakan stomata daun mangga (Mangifera indica) yang tumbuh di pinggir jalan yang banyak dilalui kendaraan bermotor lebih besar dibanding di Cagar Alam.Kata kunci: stomata, Mangifera indica Abstract. The study aims to determine the stomata of the leaves of mango (Mangifera indica) in areas with different environmental conditions. The research is descriptive analysis. Sampling was done through a random survey in Pananjung Pangandaran Nature Reserve and one of the roads in Pangandaran Sub District, Pangandaran District. The observed parameters are the density and stomatal damage. Moreover measured the leaf area, weight of the particles of dust off the leaves, as well as some physical environmental factors such as light intensity, temperature, and humidity. The results showed that the leaf stomatal density and damage to mango (Mangifera indica), which grows in the street that many passed the motor vehicle is greater than in the Nature Reserve.Keywords: stomata, Mangifera indica
Isolasi dan Identifikasi Jamur Patogen pada Air Sumur dan Air Sungai di Pemukiman Warga Desa Karangwangi, Cianjur, Jawa Barat Indrawati, Ida; Fakhrudin, Sarah Dewi
Jurnal Biodjati Vol 1 No 1 (2016): Jurnal Biodjati, Volume I, Nomor 1, November 2016
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v1i1.1017

Abstract

Abstrak. Penelitian Isolasi dan Identifikasi Jamur Patogen pada Air Sumur di Wilayah Pemukiman Warga RW 05-08 dan Air Sungai Cilaki, Desa Karangwangi, Cianjur, Jawa Barat, bertujuan untuk melihat keanekaragaman jamur patogen yang terdapat pada air sumur RW 05-08 dan air sungai Cilaki. Metode yang digunakan dalam penelitian ini adalah metode deskriptif eksploratif untuk menentukan lokasi pengambilan sampel. Dilakukan pengenceran dari sampel air kemudian dilakukan metode pour plate dengan medium Agar Sabouraud lalu diinkubasi selama 72-96 jam. Koloni jamur dihitung dengan metode Total Plate Count (TPC). Identifikasi dilakukan secara makroskopis dengan melihat bentuk dan warna koloni jamur, serta secara mikroskopis dengan cara moist chamber. Hasil jumlah koloni jamur patogen terbanyak ditemukan pada sumur RW 06. Hasil penanaman seluruh sampel air sumur dan sungai, ditemukan 9 spesies jamur patogen, genus Penicillium sebanyak 2 spesies, Trichophyton sebanyak 3 spesies, Geotrichum sebanyak 2 spesies, Microsporum dan Lichtheimia masing-masing sebanyak 1 spesies. Dari hasil yang didapat, disimpulkan bahwa air sumur di wilayah RW 05-08 dan air sungai Cilaki di Desa Karangwangi kurang baik jika digunakan secara langsung karena banyaknya jamur patogen yang terkandung di dalamnya. Kata Kunci: Jamur, Sumur, Sungai, Total Plate Count. Abstract. Isolation and Identification of Pathogenic Fungi on Well Water Territory Residential Residents RW 05-08 and Cilaki River Water, Karangwangi village, Cianjur, West Java research, aiming to see the diversity of fungal pathogens found in well water RW 05-08 and Cilaki river water. The method used in this study is descriptive exploratory method for determining the location of sampling of water in wells in RW 05 to RW 08 and Cilaki river. Do dilution of water samples and then performed pour plate method with Sabouraud agar medium and incubated for 72-96 hours. Fungal colonies which have grown calculated by the method Total Plate Count (TPC). Identification is done macroscopically by looking at the shape and color of fungal colonies and microscopically by creating a wet media room (moist chamber). The result is the highest number of colonies of pathogenic fungi found in well water samples RW 06. From the planting around the wells and river water samples, found as many as nine species of fungal pathogens, with as many as two species of the genus Penicillium, Trichophyton many as three species, Geotrichum much as 2 species , Microsporum and Lichtheimia each as much as one species. From the results obtained, it can be concluded that water wells in the area RW 05-08 and Cilaki river water in Karangwangi village less well if used directly because of the large number of pathogenic fungi colonies contained there in.Keywords : Pathogenic Fungi, Water, Well, River, Pour Plate, Total Plate Count, Moist Chamber
Aktivitas Harian Lutung Jawa (Trachypithecus auratus sondacius) di Kawasan Taman Buru Masigit Kareumbi Jawa Barat Santono, Doni; Widiana, Ana; Sukmaningrasa, Sekarwati
Jurnal Biodjati Vol 1 No 1 (2016): Jurnal Biodjati, Volume I, Nomor 1, November 2016
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v1i1.1031

Abstract

Abstrak. Lutung Jawa (Trachypithecus auratus sondaicus) merupakan salah satu bagian dari total keanekaragaman hayati Indonesia yang terdegradasi secara terus menerus disebabkan oleh kehilangan habitat dan perburuan liar. Taman Buru Gunung Masigit Kareumbi merupakan salah satu kawasan yang banyak dihuni oleh primata, yang salah satunya adalah Lutung Jawa. Primata-primata yang berada di kawasan ini khususnya Lutung Jawa selalu di buru oleh pemburu liar yang diperuntukan untuk dijual belikan bahkan digunakan untuk obat-obatan oleh pihak-pihak yang tidak bertanggung jawab terhadap keberadaan jenis primata ini. Taman Buru Gunung Masigit Kareumbi terletak di Kabupaten Garut, Sumedang dan Bandung dengan luas 12,420,70 ha. Tujuan Penelitian ini adalah untuk mengetahui aktivitas harian Lutung Jawa di Kawasan Taman Buru Gunung Masigit Kareumbi Jawa Barat. Metode yang digunakan dalam pengamatan ini adalah  menggunakan metode  Adlibitum untuk mencatat setiap perilaku yang dikerjakan atau teramati selama penelitian dan metode scan sampling yaitu pencatatan aktivitas satwa seluruh individu dalam kelompok dengan menggunakan interval waktu, Analisis data pada penelitian ini menggunakan metode deskriptif. Hasil penelitian aktivitas harian Lutung Jawa yang berada di Taman Buru Masigit Kareumbi yang memiliki titik koordinat 06°94’36’’LS dan 107°93’95’’BT dan berada pada ketinggian 1,259 mdpl ini menunjukan bahwa aktivitas harian yang sering dilakukan adalah aktivitas lokomosi yaitu dengan persentase keseluruhan sebesar 25,20%, sedangkan untuk aktivitas terendahnya adalah aktivitas urinasi dengan persentase sebesar 1,94%. Kata Kunci : Lutung Jawa (Trachypithecus auratus sondaicus), Gunung Masigit  Kareumbi Jawa Barat, metode adlibitum dan scan sampling, aktivitas. Abstract. Java langur (Trachypithecus auratus sondaicus) is one part of the total biodiversity of Indonesia is continuously degraded due to loss of habitat and poaching. Hunting Park Mount Masigit Kareumbi is one area that was inhabited by primates, one of which is a Java monkey. Primates are in the region especially Java monkey is always in hurry by poachers who intended to dijualbelikan even used for medicine by parties who are not responsible for the existence of this primate species. Hunting Park Mount Masigit Kareumbi located in Garut, Sumedang and Bandung with extensive 12.420.70 ha. The study objective was to determine the daily activities in the Java monkey Hunting Park area of Mount Masigit Kareumbi West Java. The method used in this observation is adlibitum method to record any behavior that done or observed during the study and scan sampling methods that record all activity of individual animals within a group by using a time interval, Analysis of the data in this study using descriptive methods. The results Javanese monkey daily activities that are in Hunting Park Masigit Kareumbi which has the coordinates 06°94'36 ‘’LS and 107°93'95’’BT and an altitude of 1,259 m above sea level shows that daily activity is often done with the locomotion activity overall percentage of 25.20%, while the lowest activity was urinary activity with a percentage of 1.94%.Keywords: Java monkey (Trachypithecus auratus sondaicus), Mount Masigit Kareumbi West Java, adlibitum and scan sampling methods, activities.

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