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INDONESIA
Jurnal Biodjati
Published by Universitas Islam Negeri Sunan Gunung Djati Bandung
ISSN : 25481606     EISSN : 25414208     DOI : 10.15575/biodjati
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences
Arjuna Subject : -
Articles 482 Documents
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The Growth of Oyster Mushroom on Ramie Chip Waste-Derived Media was Accelerated by Rice-washed Water Budiono, Ruly; Washfanisa, Handina Alya; Mutaqin, Asep Zainal; Kusmoro, Joko; Nurzaman, Mohamad; Setiawati, Tia; Hasan, Rusdi
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.33433

Abstract

Rice-washed water is wastewater resulted from the rice cleaning process that is rich in nutrients. The use of ramie chip waste can be considered a potential substrate due to its lignocellulosic nature, similar to other wastes studied. This study aimed to examine the effect of rice-washed water on the mycelium and pinhead growth of white oyster mushrooms grown in the ramie chip waste as the growing medium. The experiment was conducted for three months using a completely randomized design with four rice-washed water treatments and six replications. The rice-washed water concentration treatments were 0% (K0), 25% (K1), 50% (K2), and 100% (K3), which were added to the ramie chip waste as the growing medium for white oyster mushroom. The time of mycelium propagation velocity, the time the first pinhead appearance, the number of mushroom bodies, and the largest cap diameter were observed. The results showed that ramie chip waste-derived media was suitable to cultivate white oyster mushrooms. Adding 100% rice-washed water to the media significantly affected the speed of mycelium propagation (16.51 days) compared to control (30 days) and the appearance of the first pinhead (31.5 days) compared to control (38.5 days) as well as the number of fruiting bodies of mushroom (8.8) compared to control (6.2). However, the rice-washed water application did not significantly affect the diameter of the largest mushroom cap.
Analysis of Bacterial Diversity In Temple Bricks Using Phenetic Numeric Taxonomy Method Alifia, Luluk; Zulaika, Enny; Soeprijanto, Soeprijanto
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.33476

Abstract

Temple bricks-based constructions are known to often face various challenges, including physical, chemical, and biological weathering. Previous studies identified various biological factors contributing to brick weathering including exudate produced by microorganisms, such as bacteria. In addition, bacteria often live synergistically and antagonistically with other species, exhibiting diverse morphological and physiological traits (bacterial diversity). Various methods have been developed to explore bacterial diversity, with phenetic numerical taxonomy being the most popular. Therefore, this study aims to determine bacterial diversity on the surfaces of temple bricks using phenetic numerical taxonomy method. Bacterial isolation was carried out aseptically, followed by labeling and transferring the isolate to the laboratory for further tests. The tests carried out included morphological characterization, biochemical assays, physiological reactions, and potential enzymatic activities. Subsequently, dendrogram was constructed using MVSP (Multi-Variate Statistical Package) software with isolated grouping based on the Unweighted Pair Group Method Averages (UPGMA) algorithm. The similarity between isolates was analyzed using the Simple Matching Coefficient (SSM) similarity value. The dendrogram analysis revealed the presence of 3 clusters namely A (4 isolates), B (1 isolate), and C (2 isolates), with a similarity index of 0,543 to 0,857. Clusters A, B, and C had a similarity index of ≤0.700, indicating the occurrence of distinct species in each cluster. Based on the profile-matching results of key characters, the 7 bacterial isolates were identified as belonging to the genera Bacillus, Corynebacterium, and Mycobacterium.
Effect of Black Soldier Fly Frass (BSFF) on The Growth and Yield of Cayenne Pepper (Capsicum frutescens L.) Amelia, Rizki Nor; Haris, Amnan; Muliana, Putri Dinda; Fauziyyah, Istiqomah Ifnan; Damayanti, Tiara; Nisa, Jauha Khoirun; Rafidah, Zahra
Jurnal Biodjati Vol 9 No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.33656

Abstract

Cayenne pepper is a leading horticultural commodity in In­donesia. However, the productivity and quality produced have not met market expectations. One of the contributing factors is the low quality of the soil. For this reason, switching from inorganic fertilizers to or­ganic fertilizers, such as Black Soldier Fly Frass (BSFF), is necessary because it can provide several nutrients crucial in improving the soil's physical, chemical, and biological properties. In this study, the formu­lation of BSFF was tested for its impact on cayenne pepper's growth response and yield. The research was arranged in a completely ran­domized design consisting of three treatments with three repetitions, namely P1 (100 grams of BSFF biofertilizer), P2 (250 grams of BSFF biofertilizer), and P3 (500 grams of BSFF biofertilizer). The results showed an interaction between doses of BSFF biofertilizer on plant height, physical conditions (color and size), number, and wet weight of cayenne pepper; which P3 gave the most optimal growth response and yield. In contrast to these results, all treatments reduced vitamin C to the high level of N nutrients in BSFF biofertilizer. Therefore, fur­ther research is needed to find the right dosage (below 100 grams/500 grams of media) so that cayenne pepper has a high vitamin C.
Analysis of the Molecular Structure of Lipase-Dependent Chaperone from Ralstonia pickettii Strain BK6 Azwar Syah, Muhamad; Ambardini, Sri; Jamili, Jamili; Muzuni, Muzuni; Trisandy, Darul
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.33770

Abstract

Several biotechnology industries are exploring the characteristics of lipase-dependent chaperones due to their distinctive biochemical traits. This study aimed to employ bioinformatics to analyze the molecular structure of Ralstonia picketii BK6's lipase-dependent chaperon (LipRM). The sequence mapping and amino acid distribution were examined using BioEdit (version 7.0.9.1). SignalP 5.0 and Interpro are employed for signal peptide detection, whereas Swiss-Model and VMD 1.9.2 are used for molecular dynamics modelling. The results showed that the Shine-Dalgarno sequence was discovered in the LipRM promoter, seven nucleotides upstream of the initiation codon (AUG) with the 5'-AGGAGA-3', and has a terminator region that facilitates the formation of a secondary structure. The protein's 3D structure prediction results indicate differences in the alpha helix chains (residues 166-174 and 254-271) between LipRM and the reference lipase. LipRM's molecular structure comprises a detachable signal peptide, and with variations in helix alpha chain conformation and ligand geometry.
Effect of Plant Growth Regulators (PGRs) on Biomass and Flavonoid Production of Talinum paniculatum Callus Culture Kaban, Sarah Mega Pratenna; Restiani, Ratih; Aditiyarini, Dwi
Jurnal Biodjati Vol 9 No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.33788

Abstract

Talinum paniculatum Jacq (Gaertn), locally known as Javanese Ginseng, is an essential medicinal plant characterized by high flavonoid content with antioxidant and antimicrobial activities. Compared to other natural sources, a larger number of T. paniculatum leaves is required to obtain high amounts of flavonoid for drug development. In this context, in vitro culture can be used to increase flavonoid production in a controlled condition without reducing plant population. The concentration of Plant Growth Regulators (PGRs) is capable of influencing biomass and flavonoid accumulation in callus culture from several species. Despite these benefits, there is limited information about the optimal concentration of PGRs for flavonoid production from T. paniculatum callus culture. Therefore, this study aimed to investigate the optimum concentration of 2,4-D and kinetin for callus growth, as well as flavonoid production from T. paniculatum. The investigation was carried out using a completely randomized design (CRD), where young leaves explants were cultured on MS media supplemented with various concentrations of 2,4-D (0, 0.5, 1, 2) mg/L and kinetin (0, 0.5, 1, 2) mg/L for callus and flavonoid production. The results showed that the highest callus biomass of 0.105 g was produced from MS medium with 2 mg/L of 2,4-D and 1 mg/L of kinetin. Meanwhile, the highest total flavonoid content of 25.66 mg QE/g DW was produced from media supplemented with 1 mg/Lof 2,4-D and 2 mg/L of kinetin. These results showed that different combinations of 2,4-D and kinetin were required to increase biomass and flavonoid production. In conclusion, this study provided valuable information for the development of bioactive compounds through in vitro culture.
Antioxidant Activity and Pancreatic Lipase Inhibition of Curcuma aeruginosa Roxb Rhizome Fractions Suharsanti, Ririn; Wahyuono, Subagus; Yuniarti, Nunung; Astuti, Puji
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.33900

Abstract

Antiobesity with a lipase inhibitor mechanism will block the hydrolysis of triglycerides into fatty acids and glycerol, while antioxidant compounds are very useful in conditions of obesity to prevent excess damage from degenerative diseases. Curcuma aeruginosa Roxb extract has been proven to have the effect of inhibiting pancreatic lipase so further effects will be seen at the fraction level. The purpose of this research is to investigate the phytochemical components ,antioxidant activity and pancreatic lipase inhibition of Curcuma aeruginosa Roxb fractions. Ethanolic extract of the Curcuma aeruginosa Roxb rhizome was separated using the solid-liquid chromatography with 3 different solvents (n-hexane, ethyl acetate, ethanol) to give n-hexane (HF), ethyl acetate (EAF), and ethanol (EF), and the insoluble (IF) fractions. Each fraction detected phenolics, flavonoids, alkaloids, tannins, saponins, triterpenoids/steroids. EF has the highest total flavonoid and phenolic content. Antioxidant activity of all fractions were measured using DPPH reduction, ABTS, and FRAP methods. The best antioxidant activity of all fractions using the DPPH method was EF with IC50 21.93 ± 3.39µg/mL, ABTS method was HF with IC50 24.56±1,03 µg/mL and FRAP method was IF with IC50 20.79±1,03 µg/mL. Totals of phenolics and flavonoids in EF strongly support the antioxidant activity of the DPPH method. The highest inhibition of pancreatic lipase was found in EAF at 35.16±0.24 % (100 µg/ml). There was significant difference between EAF and xenical (orlistat) (p
Diversity and Abundance of Soil Arthropods in Terrestrial Area of Situ Lengkong Panjalu, West Java, Indonesia Adnan, Bahana Aditya; Kurniawati, Tiktiek; Trianto, Manap
Jurnal Biodjati Vol 9 No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.33980

Abstract

Soil arthropods play an important role in nutrient cycling and maintenance of soil structure. Thus, their abundance and diver­sity can indicate the biological quality of the soil. Due to different soil management practices, arthropods are also very sensitive to en­vironmental changes. This study aims to analyze the abundance and diversity of soil arthropods and the environmental factors that sup­port the abundance of soil arthropods in terrestrial of Situ Lengkong Panjalu, West Java, Indonesia. The methods used to obtain samples of soil arthropods are pitfall trap. Soil arthropod data were analyzed to determine the Shannon diversity index (H'), Margalef richness index (R), and Evenness index (E). The effect of abiotic environmental fac­tors was analyzed using Principal Component Analysis (PCA). A total of 1263 arthropods were found in Situ Lengkong Panjalu, belonging to 11 orders, 24 families, and 32 morphospecies. The current work determined the scores of H'= 2.08, R = 3,08, and E = 0.72 of soil arthropods in Situ Lengkong Panjalu, West Java, Indonesia. Results revealed that humidity is one such environmental parameter affecting the presence of soil arthropods in Situ Lengkong Panjalu, West Java, Indonesia. This research can be used as a guide in validating and conservation of the habitat of soil arthropod species on West Java
Fish Community Structure in The Coastal Mangrove Ecosystem of Cemara Kulon Village Indramayu Regency, Indonesia Herawati, Titin; Pauwwaz, Muhamad; Zahidah, Zahidah; Apriliani, Izza Mahdiana; Yustiati, Ayi
Jurnal Biodjati Vol 9 No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.34123

Abstract

 The mangrove ecosystem of Cemara Kulon Village has the potential for fishery resources which has decreased in line with various anthropogenic activities. This study aims to determine the composition of fish and analyze the status of the fish community structure and its relation to the physical-chemical aspects of the waters in the mangrove ecosystem of Cemara Kulon Village. This research was conducted over a period of three months  in the mangrove ecosystem of Cemara Kulon Village. The method used in this research was  descriptive quantitative purposive and composite sampling at 3 research stations. Parameters observed in this study were relative abundance, diversity, uniformity, dominance, and the relationship between community structure and aquatic physico-chemical parameters. During the study, 508 fish were caught, consisting of 20 species from 16 families. The value of diversity was in the moderate category with a ranged of 1.87-2.50; the uniformity value was in the moderate category with a ranged of 0.41-0.46 and the dominance value was in the low category with a ranged of 0.11-0.22.   Based on the results of the Canonical Correspondence Analysis (CCA) revealed that fish diversity was affected by light transparency and DO, the uniformity of fish was  affected by temperature, and the dominance of fish was affected by ammonia. The fish community structure was categorized as unstable which was influenced by water quality.
In Vitro Evaluation of Green Tea Extract's Influence on Local Sheep Rahmatillah, Ririn Siti; Ramdani, Diky; Hernaman, Iman; Jayanegara, Anuraga
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.34573

Abstract

In recent years, the exploration of natural supplements such as green tea extract and their potential benefits in livestock nutrition has gained considerable attention among researchers and agriculturalists. Green tea extract contains a high concentration of bioactive substances, such as polyphenols and catechins. This study aimed to explore the effects of varying doses of green tea extract on rumen fermentation parameters through an in vitro trial using sheep rumen liquids. A completely randomized experimental design was utilized to test the effect of 4 different doses of green tea extract treatments (0, 140, 280, and 560 mg/kg) on in vitro Hohenheim gas test method. The current study shows no significant effect of administering green tea extract at various doses on total gas production, total gas production per in vitro dry matter and organic matter degradability, volatile fatty acid per in vitro dry matter and organic matter degradability, methane production per inv vitro dry matter and organic matter degradability of green tea extract. Although no significant effects were observed on the investigated parameters, this study provides important insights in the understanding of the potential use of green tea extract in modulating fermentation processes in the ruminant’s stomach. In conclusion, the findings of this study underscore the impact of GTE on gas production regulation, highlighting its potential implications for gastrointestinal health
Ganoderma Diversity and Its Interactions with Hosts and Neighbouring Insects in Gunung Walat Educational Forest Mayra, Mahreza Tadsa; Chan, Carey; Abdurrahman, Naufal Hafiz; Putra, Ivan Permana
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.36749

Abstract

Ganoderma spp. is a genus of macrofungi in the Basidiomycota, comprising both saprophytic and phytopathogenic species. Ganoderma spp. prefers woody substrates and can attack various trees in the forest. While it often acts as a phytopathogenic fungus, it also serves an ecological role for surrounding insects, providing food and shelter. This study aims to determine the interactions and preferences of Ganoderma spp. with its hosts and to observe the interactions between Ganoderma spp. and surrounding insects. Research on these interactions was conducted through scan sampling exploration in the Gunung Walat Educational Forest. Identification of fungi, trees, and insects was based on morphological characteristics. A total of 155 fruiting bodies of Ganoderma were found. Three species of Ganoderma were successfully collected and described morphologically, both macroscopically and microscopically. Ganoderma spp. were found infecting Agathis sp., Schima wallichii, and Pinus merkusii trees. The fruiting bodies of Ganoderma spp. interacted with insects such as larvae, Trichomyrmex destructor ants, and Eumorphus marginatus beetles, which used the fruiting bodies as breeding and shelter sites

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