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INDONESIA
Jurnal Sain Veteriner
Published by Universitas Gadjah Mada
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Veterinary
Arjuna Subject : -
Articles 824 Documents
 72   73   74   75   76 
Deteksi Bakteri Patogen Pada Daging Ayam Broiler Sebagai Skrining Foodborne Diseases Di Kabupaten Jember Suswati, Enny; Supangat, Supangat; Lutfadaturroifa, Alya Wafaul; Pratama, Dava Rizky
Jurnal Sain Veteriner Vol 42, No 3 (2024): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.86084

Abstract

Chicken meat is a source of animal protein that is very popular because of its high nutritional value, easy to obtain and cheap. However, as production increases, certain drugs and feed additives become essential for disease prevention, treatment, and growth promotion. The negative effect of using antimicrobial drugs is that they accumulate in animal tissues as residues and eventually become part of the food pyramid, subsequently impacting human health.A total of 45 broiler chickens were purchased from broiler meat sellers in 9 traditional markets in Jember. We took five broiler chicken meat randomly from each market. Samples have identified the type of contaminant bacteria using Salmonella Chromogenic Agar and Mannitol Salt Agar. The results of this study showed that broiler chicken meat samples were contaminated with E. coli (45/45 (100%)), Staphylococcus aureus (43/45 (95.56%)), Salmonella spp. (38/45 (84.44%)) and Proteus spp. (14/45 (33.33%)). All broiler meat samples purchased at the Jember traditional market were contaminated with at least 1 type of bacteria, namely E. coli. The index of bacterial contamination is between 0.25 – 1. The most common contamination patterns are S. aureus, E. coli and Salmonella spp., with a bacterial contamination index of 0.75.
Case Study: Colibacillosis and Coccidiosis in Broiler Chicken Farm Hartady, Tyagita Tyagita; Subagio, Reza Taufik; Khairani, Shafia
Jurnal Sain Veteriner Vol 42, No 3 (2024): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.89079

Abstract

Colibacillosis and coccidiosis are diseases that often infect broiler chicken. Escherichia coli is an opportunistic commensal bacterium. Colibacillosis often occurs as a secondary infection from other infectious diseases, such as coccidiosis. Coccidiosis is a digestive disease caused by Eimeria sp. Colibacillosis and coccidiosis cause stunting in chicken. This study was conducted to determine pathological features in colibacillosis and coccidiosis cases in broiler, as well as predisposing factors that can increase the risk of these diseases. The results of this study showed that colibacillosis causes pathological changes including peritonitis which is characterized by the formation of fibrinous exudate in the peritoneum, and inflammation of the air sacs. Coccidiosis causes hemorrhagic lesions in the small intestine and cecum. Water pipes that are corroded and overgrown with biofilms can become predispose factor to colibacilosis, because the biofilm layer facilitate the growth of E. coli and cause ineffective disinfection of drinking water. On the other hand, moist litter can promote Eimeria sp. oocysts sporulation; thereby increasing the risk of coccidiosis. In addition, moist litter also promote the growth of other disease agents such as bacteria and fungi.
Sebaran Lesi Skabiosis Pada Kambing Di Desa Nampar Tabang, Kabupaten Manggarai Timur Febrizki Bollyn, Yohana Maria; Tukan, Hendrikus Demon; Achmadi, Puspita Cahya
Jurnal Sain Veteriner Vol 42, No 3 (2024): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.89899

Abstract

Skabies, yang juga dikenal sebagai kudis atau skabiosis, adalah penyakit kulit yang disebabkan oleh tungau kecil bernama Sarcoptes scabiei. Tungau menggali terowongan di dalam lapisan atas kulit, menyebabkan gatal-gatal, kerusakan kulit, dan ketidaknyamanan pada hewan yang terinfeksi. Skabies dengan cepat menyebar di antara hewan yang tinggal dalam kelompok atau kandang yang sama. Infeksi skabies pada kambing dapat menyebabkan penurunan berat badan dan performa yang buruk, biaya pengobatan yang besar serta stres berkepanjangan pada ternak kambing. Penelitian ini bertujuan untuk mengetahui sebaran lesi Skabies (Sarcoptes scabiei) pada kambing di Desa Nampar Tabang Kabupaten Manggarai Timur. Hasil penelitian menunjukkan sebaran lesi pada kambing dengan persentase distribusi pada wajah sebesar 64,58%, leher 1,56%, punggung sebesar 18,75%, abdomen sebesar 6,25%, region kaki belakang sebaran lesi sebesar 3,12% dan ekor sebesar 5,73%. 
High Risk Period (HRP) Kasus Penyakit Mulut dan Kuku Pasca Wabah pada Peternakan Sapi Perah di Kabupaten Malang Basri, Chaerul; Widyadhari, Atsmarina; Sudarnika, Etih
Jurnal Sain Veteriner Vol 42, No 3 (2024): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.91219

Abstract

Penyakit Mulut dan Kuku (PMK) merupakan penyakit ternak yang sangat merugikan secara ekonomi. Perhitungan periode berisiko atau high risk period (HRP) akan membantu otoritas veteriner untuk melakukan pelacakan secara efisien potensi wabah sekunder atau penyebaran virus dari kasus indeks. Penelitian ini dilakukan untuk menganalisis perhitungan HRP pada peternakan sapi perah di Kabupaten Malang berdasarkan variabel 1) pengetahuan peternak mengenali gejala klinis PMK, 2) waktu pelaporan peternak, 3) waktu petugas datang menangani pelaporan, dan 4) waktu yang dibutuhkan untuk konfirmasi uji laboratorium. Survei dilakukan pada 126 peternak pada bulan Juli-Agustus 2023. Ukuran sampel dihitung berdasarkan asumsi tingkat kepercayaan 95%, prevalensi dugaan 13%, tingkat kesalahan 6% menggunakan software WinEpiscope 2.0. Data dianalisis secara deskriptif. Peternak sapi perah di Kabupaten Malang memiliki pengetahuan yang baik tentang gejala klinis PMK meski ada beberapa ciri yang luput dan perlu ditingkatkan pemahamannya. Sebanyak 98% peternak segera melakukan pelaporan setelah mengamati adanya gejala klinis PMK pada ternaknya. Kecepatan petugas dalam menanggapi pelaporan sudah baik yakni seketika pada hari pelaporan dilaporkan dan pengujian laboratorium yang segera dilakukan untuk mengonfirmasi penyakit PMK dapat membuat probabilitas HRP 7 hari 91% (SK: 0,87-0,95),  HRP 14 hari 7% (SK: 0,04-0,11) dan HRP 21 hari 2% (SK: 0,01-0,05). Pengetahuan mengenai gejala klinis dan kesadaran peternak dalam melakukan pelaporan PMK di Kabupaten Malang sudah baik, namun perlu ditingkatkan. Upaya peningkatan peran peternak dalam deteksi dini dapat dilakukan dengan intervensi berupa penyuluhan dan membangun kedekatan dengan petugas lapangan.
Prevalensi dan Tingkat Resistansi Silang Golongan Kuinolon Pada Escherichia coli Asal Usap Kloaka Ayam Layer Hidayah, Nur Nur; Palupi, Maria Fatima; Ariyani, Novida Ariyani; Indryana, Indryana Indryana; Jannah, Anna Miftahul; Komariyah, Siti Komariyah; Sari, Rosana Anita; Ambarwati, Ambarwati Ambarwati; Rusmiati, Emi Rusmiati; Idrishanty, Nafisah Idrishanty; Fanani, Fika Asti
Jurnal Sain Veteriner Vol 42, No 3 (2024): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.92045

Abstract

Escherichia colimerupakan bakteri komensal yang digunakan sebagai parameter monitoring resistansi pada hewan maupun manusia. Selain bersifat komensal, terdapat juga E. coli patogen yang dapatmenginfeksi pada hewan dan manusia. Resistansi E. coli terhadap golongan antibiotik kuinolon berpotensi semakin meningkat. Namun demikian, belum banyak diketahui mengenai tingkat resistansi silang golongan kuinolon pada E. coli patogen khususnya pada ayam layer. Tujuan studi ini adalah untuk mengetahui prevalensi resistansi E. coli pada beberapa jenis kuinolon dan tingkat resistansi silang antar golongan kuinolon dari E. coli asal ayam layer. Sebanyak 360 isolat E. coli asal usap kloaka ayam layer arsip Balai Besar Pengujian Mutu dan Sertifikasi Obat Hewan (BBPMSOH) tahun 2022 dari delapan provinsi diuji kepekaan terhadap siprofloksasin, norfloksasin, enrofloksasin, flumekuin, serta marbofloksasin, dan diuji patogenesitasnya. Uji kepekaan dilakukan dengan menggunakan metode dilusi agar. Prevalensi E. coli resistan terhadap siprofloksasin adalah 45,00% (CI 95%; CI 39,94% - 50,16%), norfloksasin 38,33% (CI 95%; CL 33,46% - 43,45%), enrofloksasin 44,72% (CI 95%; CL 39,67% - 49,89%), flumekuin 42,22%(CI 95%; CL 37,23% - 47,38%), dan marbofloksasin 42,22% (CI 95%; CL 37,23% - 47,38%). Sebanyak 160 (44,44%) merupakan isolat E. coli multiresistan. Tingkatresistansi silang kuinolon dievaluasi dengan statistik kappa (k) berdasarkan resistansi siprofloksasin terhadap norfloksasin, enrofloksasin, flumekuin, dan marbofloksasin. Nilai (k) resistansi silang siprofloksasin terhadap empat kuinolon yang lain adalah 0,86-0,99. Hal ini menunjukkan  tingginya resistansi silang dalam golongan kuinolon. Adanya prevalensi resistansi dan silang kuinolon yang tinggi menunjukkan ancaman yang serius bagi tingkat efikasi antibiotik kuinolon.     
Histological Structure of Spleen White Rat (Rattus norvegicus L.) After Induced of Nanochitosan Preparation of Neem Leaf (Azadirachta indica A. Juss) Ethanol Extract Ningtias, Putri Lestari; Suprihatih, Teguh; Sitasiwi, Agung Janika
Jurnal Sain Veteriner Vol 42, No 3 (2024): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.92377

Abstract

Agents of immunomodulation are compounds that have the ability to balance the immune system throughout the body. The neem plant is one of the herbal plants that is often used as traditional medicine. Neem leaves (Azadirachta indica A. Juss) contain antioxidants that have the potential to be antiinflammatory, antiviral, and as an immunomodulatory agent. The constraint in giving medicine orally is the low bioavailability of drugs and the distribution of active compounds of herbal plants. This problem can be solved by packaging herbal plant extracts in nanochitosan. The purpose of this study was to analyze the effect of induced of a nanochitosan ethanol extract of neem leaves on the histological structure of the spleen tissue of female Wistar white rats. This study used a randomized design (CRD) which was divided into 3 treatment groups with 4 repetitions. Treatments include control (P0), an ethanol extract of neem leaves 2 ml/head/day (P1), and nanochitosan preparation of neem leaves 2 ml/head/day (P2). The parameters measured were the diameter of white pulp, the diameter of germinal centre, the area of white pulp, and the area of germinal centre in the spleen. Data on the diameter and the area of the white pulp and germinal centre were analyzed statistically parametrically with ANOVA with a 95% level of confidence, if the data were not normally distributed, the Kruskall Wallis nonparametric test was carried out. Data that were significantly different were carried out by Duncan’s further test. The results showed that the treatment of nanochitosan preparations of neem leaf ethanol extract had a significant difference in the diameter of the white pulp and germinal centre as well as the area of the white pulp and germinal centre (P<0,05). The conclusion of this study was that the nanochitosan preparation ethanol extract of neem leaves at a dose of 14 mg/kg BB can increase the diameter and the area of the white pulp and germinal centre.
Isolation, Identification, and Resistance Test of Escherichia coli to Antibiotics in Ujung Berung Broiler Poultry Afifa Kantamaryana, Fatia Nasyna; Balia, Roostita L; Putranto, Wendry Setiyadi
Jurnal Sain Veteriner Vol 42, No 3 (2024): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.92624

Abstract

Escherichia coli is commensal bacteria in the intestinal of broiler chickens with low virulence. However, mostly pathogenic and cause colibacillosis illness. Inappropriate use of antibiotics in broiler chickens has been widely reported. This research aims to determine the presence of E. coli contamination in the poultry environment and determine the levels of antibiotics resistance of erythromycin, doxycycline, oxytetracycline and neomycin. The research was carried out through isolation and identification of E. coli using EMB media, Gram staining, and biochemical tests. Then, an antibiotic resistance test was carried out using by Kirby-Bauer method. From a total of 10 samples (3 coops), 7 (70%) were positive for E. coli. The positive of 7 which for E. coli, 43% of the isolates came from fresh feces obtained from coops 1, 2, and 3; 43% of the isolates came from litter from coops 1, 2, and 3; and 14% of isolates from soil outside the coop 3. The antibiotic resistance results by Kirby-Bauer method showed that E. coli was 100% resistant to erythromycin, 100% to neomycin, 66.7% to doxycycline, and 66.7% to oxytetracycline throughout isolates from feces and litter. Other results showed that isolates from soil outside coop 3 showed resistance to all antibiotics. Taken together, we concluded the E. coli has been contamination which is proven by antibiotic resistance.
Prolapsus Rektum Pada Domba Dorper (Case Report: Rectal Prolapses in Dorper Sheep) Prastiwi, Artina; Adji, Dhirgo; Anggraeni, Rr. Devita; Purnomo, Agus; Ramadhani, Mungky Emma
Jurnal Sain Veteriner Vol 42, No 3 (2024): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.95250

Abstract

Prolaps rektum merupakan tonjolan jaringan rektal di luar anus. Faktor penyebab prolaps rektum pada hewan muda dan tua meliputi konstipasi, endoparasit, diare, faktor keturunan, kehilangan daya spinchter ani dan pelonggaran selaput lendir rektum. Hewan kasus adalah tiga ekor domba Dorper berasal dari Wonosari, Gunungkidul dengan keluhan prolaps rektum selama 3-4 hari, nafsu makan menurun dan domba masih aktif. Domba pertama betina 50 kg berusia 2.5 tahun, domba kedua betina 30 kg berusia 5 bulan, dan domba ketiga jantan 35 kg berusia 5 bulan. Pemeriksaan fisik menunjukkan gejala klinis primer prolapsus rektum berupa massa silindris memanjang yang menonjol melalui lubang anus, bagian proksimal rectum berwarna merah muda sedangkan bagian distal berwarna merah, serta terdapat peradangan pada mukosa rektum. Tindakan operasi dilakukan untuk reposisi rektum. Premedikasi menggunakan Acepromazine, dilanjutkan injeksi anestesi epidural menggunakan Lidocaine. Anestesi lokal Lidocaine line block diinjeksikan di sekitar anus. Rektum dimasukkan perlahan secara manual, kemudian dilakukan penjahitan di sekeliling anus dengan pola purse string agar rektum tidak kembali prolaps. Terapi post operatif diberikan injeksi antibiotik broad spectrum Amoxicillin dengan sediaan 150 mg/ml (1 ml/10kg BB) dan injeksi antiinflamasi (NSAIDs) Flunixin Meglumine 50 mg/ml (0,02-0,04 ml/kg BB; q12h). Domba Dorper jantan dipotong karena mengalami prolaps rektum berulang dan lemah. 
Isolation of Aspergillus from edible-nest swiftlet feces in bird houses of Central Java Ningrum, Siti Gusti; Ginting, Eykel Arief Fortuna; Rahmawati, Indra
Jurnal Sain Veteriner Vol 42, No 3 (2024): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.95507

Abstract

Five edible-nest swiftlet houses in Central Java were sampled for fecal specimens. The samples were obtained using a sterile container to maintain aseptic conditions. Subsequently, the samples were mixed thoroughly in a tube containing sterile saline and allowed to incubate for a duration of 30 minutes. One milliliter of supernatant was placed onto Potato Dextrose Agar (PDA) with chloramphenicol (50 mg/L) and incubated at a temperature of 25 ºC for a duration of one week. The results showed that all samples had a high percentage of Aspergillus niger (100%), Aspergillus flavus (100%), and Aspergillus fumigatus (100%) isolates. The findings suggest that edible-nest swiftlet houses in Central Java can serve as a potential reservoir and conduit for fungal infection and transmission.
Histopathological and Immunohistochemical Evaluation of African Swine Fever Pigs in Bali Province Septiani, Monica; Juniantito, Vetnizah; Priosoeryanto, Bambang Pontjo
Jurnal Sain Veteriner Vol 42, No 3 (2024): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.98994

Abstract

African Swine Fever (ASF) is a disease affecting pigs, caused by a double-stranded DNA virus that is not transmissible to humans or other animals. It leads to significant economic losses due to high morbidity and mortality rates, particularly in densely populated pig regions like Bali Province. During the ASF outbreak from June to December 2023, this study conducted histopathological research on clinical samples. Biological materials from twelve pigs confirmed positive for ASF via qPCR examination were histopathologically analyzed. Tissue samples from various organs underwent processing and examination using hematoxylin and eosin staining. Immunohistochemistry (IHC) was employed to detect the ASF virus’s p54 protein. Clinical symptoms, anatomical pathology, and histological examination revealed characteristic acute ASF lesions. Immunohistochemistry consistently showed p54 viral antigen distribution in mononuclear cells/macrophages across various organs, with the spleen and lymph nodes being dominant sites in 12 pigs (100%). This comprehensive study demonstrates the effectiveness of IHC in detecting the ASF virus and characterizing its histopathology.Keywords: ASF; histopathology; immunohistochemistry

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