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Jurnal Kedokteran Hewan

JKH
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Published by Universitas Syiah Kuala

ISSN : 1978225X EISSN : 25025600 DOI : 10.21157

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian Veterinary Medical Association (PDHI). Jurnal Kedokteran Hewan is a double-blind review process journal that has been accredited by National Journal Accreditation (ARJUNA), with second grade (Sinta 2), Number: 200 / M / KPTS / 2020. This journal has been registered in the Indonesian Publication Index (IPI), Google Scholar, Sinta, World Cat, Directory of Open Access Journals (DOAJ), EBSCO, Copernicus, Microsoft Academic, and other scientific databases. Jurnal Kedokteran Hewan receives scientific manuscripts in veterinary sciences (veterinary miscellaneous): anatomy, histology, physiology, pharmacology, parasitology, microbiology, epidemiology, veterinary public health, pathology, reproduction, clinic veterinary, aquatic animal disease, animal science, and biotechnology.

Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)

Articles 901 Documents

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OVSYNCH DAN INSEMINASI BUATAN PADA INDUK KUDA WARMBLOODYANG DIINDUKSI OVULASI DENGAN HUMAN CHORIONIC GONADOTROPIN DOSIS JAMAK Amrozi A; Ligaya ITA Tumbelaka; Ade Ocktaviani; Bondan Achmadi; Juli Melia
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengamati pola pertumbuhan folikel dan keberhasilan inseminasi buatan dengan semen cair pada induk kuda warmblood yang disinkronisasi estrus dan ovulasi (ovsynch). Induk kuda berjumlah lima ekor berumur 6-18 tahun digunakan dalam penelitian ini. Sinkronisasi estrus dilakukan pada induk kuda yang memiliki korpus luteum berdiameter minimal 3,0 cm dengan injeksi prostaglandin 7,5 mg secara intramuskular. Induksi ovulasi dilakukan dengan memberikan hCG 1500 IU secara intravena 48 jam setelah sinkronisasi estrus dan diulang setiap 24 jam sampai terjadinya ovulasi folikel (dosis jamak) yang diamati dengan ultrasound. Inseminasi buatan dilakukan berulang mengikuti setiap pemberian hCG sampai terjadinya ovulasi dengan dosis inseminasi 1,5x109 spermatozoa. Sinkronisasi estrus dan ovulasi dengan menggunakan hCG dosis jamak menghasilkan ovulatori dominan folikel berdiameter 4,81±0,92 cm dan korpus rubrum berdiameter 3,82±0,45 cm serta menghasilkan 60% kebuntingan. Kesimpulan sinkronisasi ovulasi dengan pemberian hCG dosis jamak pada kuda warmblood yang diinseminasi buatan dengan semen cair efektif menghasilkan kebuntingan yang tinggi.

EVALUATION OF RAT LEYDIG CELL CULTURE COLLECTED WITH NYCODENZ GRADIENT IN PRODUCING TESTOSTERONE IN VITRO Ekayanti Mulyawati Kaiin; Wahono Esthi Prasetyaningtyas
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

The aim of this study was to evaluate the ability of rat’s Leydig cells collected with Nycodenz gradient in producing testosterone in vitro. Leydig cells were collected using 5 column of Nycodenz gradient (4, 8, 10, 12, and 15%) and cells were evaluated regarding its concentration, viability, and purity of Leydig cells. Media used to cultured Leydig cells were Dulbecco’s Modified Eagle’s Medium (DMEM)+10% newborn calf serum (NBCS); DMEM+10% NBCS+2,5 IU/mL human chorionic gonadotrophin (hCG); DMEM+10% NBCS+ 5 µg/mL insulin, 10 µg/mL transferrin, and 5 µg/mL Se (ITS); DMEM+10% NBCS+hCG+ITS at 5% CO2 incubator with temperature of 37.5° C for 3 days. Culture medium was collected every day for testosterone analysis with enzyme-linked immunosorbent assay (ELISA). By adding ITS to the medium, Leydig cells concentration was significantly increased (8.92x106 cells/mL) compared to medium with serum (7.74x106 cells/mL) or hCG (7.68x106 cells/mL) (P0.05). ITS and hCG in medium significantly increased Leydig cells concentration (10.40x106 cells/mL) at day 3 of culture (P0.05). The result of parallelism test showed that the assay obtained good validity to measure testosterone concentration in culture medium. Testosterone in medium was detected at 1.80-2.60 ng/mL at day 1 of culture. In conclusion, Leydig cells collected with Nycodenz gradient had no effect to testosterone secretion from Leydig cells in vitro.

PENURUNAN KADAR PROGESTERON SERUM DAN KOMPONEN MATRIKS EKSTRASELULER DAN SELULER KULIT SEBAGAI INDIKATOR PENUAAN PADA TIKUS Safrida s; Nastiti Kusumorini; Wasmen Manalu; Hera Maheshwari
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui kadar kolagen kulit (matriks ekstraseluler), jumlah sel (kadar DNA), aktivitas sintetik (kadar RNA) pada jaringan kulit, serta kadar hormon progesteron pada berbagai tingkatan umur tikus, dan memperoleh umur tikus ovariektomi yang cocok digunakan sebagai hewan model penuaan. Rancangan percobaan yang digunakan adalah rancangan acak lengkap (RAL) yang terdiri atas sembilan kelompok percobaan masing-masing terdiri atas tiga ekor tikus, yaitu umur 12 bulan (K1), 18 bulan (K2), 24 bulan (K3), 30 bulan (K4), 36 bulan (K5), umur 12 bulan dalam kondisi 1 bulan pascaovariektomi (K6), 12 bulan dalam kondisi 3 bulan pascaovariektomi (K7 ), 18 bulan dalam kondisi 1 bulan pascaovariektomi (K8), dan umur 24 bulan dalam kondisi 1 bulan pascaovariektomi (K9). Data yang diperoleh dianalisis dengan menggunakan analisis varian dan dilanjutkan dengan uji Duncan. Komponen matriks ekstraseluler dan matriks seluler menurun seiring dengan bertambahnya usia. Umur tikus normal yang cocok digunakan sebagai hewan model penuaan adalah tikus umur 24-36 bulan. Tikus ovariektomi yang cocok digunakan sebagai hewan model penuaan adalah tikus umur 12 bulan pascaovariektomi 3 bulan.

IDENTIFICATION OF COPPER-INDUCIBLE GENES IN SWORDTAIL FISH (Xiphophorus spp.) USING DIFFERENTIAL DISPLAY Dwinna Aliza; Tengku Muhammad
Jurnal Kedokteran Hewan Vol 2, No 1 (2008): March
Publisher : Universitas Syiah Kuala

Differential display technique was used to identify differentially expressed gene ofswordtail fish (Xiphophorus spp.) that induced by 1 µg/ml copper for 24 hours. From eightprimers that have been used for differential display, seven of them showed the differentialgenes which were not present in the control. The reamplification of these fragments rangingfrom below 100 to 200 bp. But only two fragments were successfully cloned and sequenced,those are H-AP10 and H-AP11. Sequence analysis showed that both of the sequences revealedhigh similarity with Homo sapiens stress-associated endoplasmic reticulum protein, a signalgenerated that induces apoptosis.Keywords: differential display, swordtail fish, copper pollution, mRNA expression, biomarker

PENGEMBANGAN TEKNIK ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) MENGGUNAKAN ANTIBODI MONOKLONAL UNTUK MENDETEKSI ANTIBODI PENYAKIT BOVINE EPHEMERAL FEVER Indrawati Sendow; R.M. Abdul Adjid; Atik Ratnawati; Muharam Saepulloh
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengembangkan teknik enzyme-linked immunosorbent assay (ELISA) untuk mendeteksi antibodi terhadap virus bovine ephemeral fever (BEF). Pada penelitian ini dikembangkan uji ELISA langsung (direct ELISA) dan tidak langsung (indirect ELISA) dengan menggunakan antibodi monoklonal (blocking ELISA). Hasil penelitian menunjukkan bahwa uji direct ELISA tidak dapat digunakan dengan baik karena terjadi positif palsu. Uji blocking ELISA bereaksi lebih baik dan dapat dikembangkan lebih lanjut untuk mendeteksi antibodi terhadap penyakit BEF. Dapat disimpulkan bahwa pengembangan teknik deteksi dini terhadap BEF dengan mempergunakan antibodi monoklonal dapat diterapkan dalam upaya pengawasan penyakit dan surveilans.

THE POTENTIAL OF TOMATO EXTRACT (Lycopersicum esculentum L.) IN INCREASING THE SERTOLI CELLS AND SPERMATOGENIC CELLS COUNT OF THE CONTRALATERAL TESTICULAR SEMINIFEROUS TUBULES OF RATS (Rattus norvegicus) THAT HAVE TESTICULAR TORSION Dasrul Dasrul; Cut Nila Thasmi; Triva Murtina Lubis; Awaluddin Awaluddin; Winaruddin Winaruddin; Rasmaidar Rasmaidar; Yola Alifa
Jurnal Kedokteran Hewan Vol 15, No 3 (2021): September
Publisher : Universitas Syiah Kuala

The purpose of this study was to analyze the potential of tomato extract (Lycopersicum esculentum L.) in increasing the Sertoli and spermatogenic cells count of the contralateral testis in white rats (Rattus novergicus) with unilateral torsion. A total of 24 male Wistar white rats, aged 3-4 months old with a weight of 180-200 g were used in this study. The rat samples were randomly divided into 4 treatment groups: Group 1 as a negative control (NC) which consisted of rats without testicular torsion and given tomato extract; Group 2 as a positive control (PC) which consisted of rats with unilateral testicular torsion 720° for 6 hours without given tomato extract; Group 3 as treatment 1 (T1) consisted of rats that were subjected to unilateral testicular torsion 720o for 6 hours and were given tomato extract at a dose of 100 mg/kg BW/day; and Group 4 as treatment 2 (T2) consisted of rats that were subjected to unilateral testicular torsion 720° for 6 hours and were given tomato extract at a dose of 200 mg/kg BW/day. Each group consisted of six rats. Tomato extract was administered orally for 30 days. The observations of the number of Sertoli cells and spermatogenic cells (spermatogonia cells, spermatocytes, and spermatids) were carried out histologically with Hematoxylin-Eosin (HE) staining and the slides were examined with a light microscope. The data obtained was analyzed by ANOVA and continued with Duncan's test. The results showed that the administration of tomato extract significantly increased (P0.05) the number of Sertoli cells and spermatogenic cells of the contralateral testicular seminiferous tubule. Giving tomato extract 100 mg/kg BW/day resulted in an increase in the number of Sertoli cells and better spermatogenic cells than tomato extract 200 mg/kg BW/day. In conclusion the administration of tomato extract after detorsion can increase the number of Sertoli cells and spermatogenic cells of the contralateral testicular seminiferous tubules in white rats subjected to unilateral torsion of 720° for 6 hours

INFLUENCES OF INCUBATION TIME AND SUCROSE CONCENTRATION ON MICE (Mus musculus L.) OOCYTE VIABILITY FOR ENUCLEATING PROCEDURE Ekayanti Mulyawati Kaiin; Muhammad Gunawan; Madihah Madihah; Ghina Nafisah
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): September
Publisher : Universitas Syiah Kuala

This study aimed to find out the optimum incubation time to complete mouse oocyte maturation at Metaphase II (MII) stage and determine the optimum sucrose concentration enabling to induce nuclear swelling for visualization that is important for enucleating process at the initial procedure of somatic cell nuclear transfer (SCNT). In this current study, mice were used as animal model. Completely randomized design was arranged, consists of 2 trials with 4 treatments and 7 replications. In the first trial, the oocytes were cultured at 0-2, 4-6, 8-10, and 12-14 h in 5% CO2 incubator at 37 C. Second, the MII oocytes obtained from previous trial were cultured in M199 medium containing different concentrations of sucrose (0, 1.5, 3, and 6%). The parameters measured were the oocyte viability at various stages, i.e germinal vesicle (GV), metaphase I (MI), anaphase/telophase I (A/T I), and metaphase II (MII), and the viability of swollen nuclear oocytes using Hoechst/PI staining. The results showed that the optimum incubation time required by oocytes to reach MII stage was 12-14 h with a percentage of 57.14±12.67%, while the optimum sucrose concentration for nuclear swelling was found at 3% with a percentage of 100±0.00%. Our findings provided preliminary results related to the maturation process of the mouse oocyte nucleus, which is meaningful for the initial procedure of SCNT.

EKSPRESI INSULIN PADA PANKREAS MENCIT (Mus musculus) YANG DIINDUKSI DENGAN STREPTOZOTOCIN BERULANG Erwin E; Etriwati E; Muttaqien M; Tri Wahyu Pangestiningsih; Sitarina Widyarini
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui ekspresi insulin pada pankreas mencit (Mus musculus) yang diinduksi streptozotocin berulang dengan pewarnaan imunohistokimia yang berguna sebagai hewan model diabetes melitus. Tiga puluh ekor mencit jantan galur Balb-C, umur 12-14 minggu dengan bobot badan 30-40 g dikelompokkan menjadi 2 kelompok perlakuan, masing-masing kelompok terdiri atas 15 ekor. Kelompok 1 (K1) diberikan pelarut streptozotocin, sedangkan kelompok 2 (K2) diberikan streptozotocin dengan dosis 40 mg/kg bobot badan dalam 50 mM natrium sitrat bufer pH 4,5 secara intraperitoneal sebanyak 0,5 ml selama 5 hari berturut-turut. Hewan percobaan dari masing-masing kelompok dieutanasia sebanyak 2 ekor pada hari ke-0, 7, 14, 21, dan 28 setelah perlakuan, selanjutnya mencit diperfusi dan dinekropsi untuk mengambil jaringan pankreas sebagai sampel pemeriksaan imunohistokimia dengan metode streptavidin peroksidase menggunakan antibodi mouse anti-insulin (1:300). Berdasarkan uji statistik menggunakan analisis varian, ekspresi insulin pada sel beta Langerhans pankreas K1 lebih tinggi dibandingkan K2 (P0,05). Waktu pengamatan dan interaksi antara kelompok dan waktu pengamatan menunjukkan perbedaan yang signifikan (P0,05). Induksi dosis rendah streptozotocin secara berulang dapat menurunkan jumlah ekspresi sel beta Langerhans pankreas yang imunoreaktif terhadap insulin.

PENENTUAN KONSENTRASI SODIUM DODECYL SULFATE DALAM PENGENCER RINGER LAKTAT-KUNING TELUR UNTUK PRESERVASI SEMEN AYAM PELUNG (Determination of Sodium Dodecyl Sulfate Concentration in Ringer Lactate-Egg Yolk Extender for Pelung Rooster Semen Preservation) Nu’man Hidayat; Cece Sumantri; Rudi Afnan
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

The objective of this experiment was to determine the addition of sodium dodecyl sulfate (SDS) to Ringer Lactate-Egg Yolk (RL-EY) extender on pelung chicken semen preservation. Semen was collected three times a week from three pelung chickens. Collected semen was evaluated macroscopically and microscopically. Only ejaculates of at least 70% sperm motility was then divided into three equal microtubes. Each of them diluted with RL-EY with ratio 90%:10%, then added with 0.00, 0.025, and 0.05% SDS respectively. The liquid semen then stored at 5 °C for 72 hours. Sperm motility and viability were observed every 12 hours. The addition of 0.025% SDS showed higher spermatozoa motility and viability (72.08±1.44% and 80.82±1.30%) which were significantly higher (P0.05) than 0.00 and 0.05% SDS addition at 24 hours of storage. There was no differences on the spermatozoa motility and viability between 0.00 and 0.05% SDS addition. The decrease of spermatozoa motility and viability was observed in 0.025% SDS addition (4.17±0.56% and 4.65±0.59%, respectively) that significantly lower compared to 0% and 0.05% SDS addition at 24 hours of storage. In conclusion, 0.025% SDS addition in a Ringer Lactate -Egg Yolk extender maintained pelung spermatozoa motility and viability better than 0.00 and 5% SDS addition.

TINGKAT FERTILISASI OOSIT DOMBA DARI OVARIUM YANG DISIMPAN PADA SUHU DAN WAKTU YANG BERBEDA SECARA IN VITRO Arie Febretrisiana; Mohamad Agus Setiadi; Ni Wayan Kurniani Karja
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui pengaruh suhu dan waktu penyimpanan ovarium terhadap tingkat fertilisasi oosit secara in vitro pada domba. Ovarium dibawa dari rumah potong hewan (RPH) dalam medium NaCl fisiologis pada suhu yang berbeda yaitu 27-28° C, 36-37° C, dan 4° C. Oosit kemudian dikoleksi dari setiap kelompok berdasarkan waktu penyimpanan yang berbeda yaitu 2-4, 5-7, dan 8-10 jam setelah domba dipotong. Oosit dikoleksi dan dimaturasi secara in vitro dalam inkubator 5% CO2, 38,5°C selama 28 jam. Oosit kemudian difertilisasi ke dalam drop spermatozoa selama 14 jam dalam inkubator CO2 5%, 38,5 C. Tingkat fertilisasi dievaluasi berdasarkan jumlah pronukleus yang terbentuk. Tingkat fertilisasi oosit yang dikoleksi dari ovarium yang disimpan pada suhu 27-28° C tidak berbeda dengan tingkat fertilisasi oosit yang disimpan pada suhu 36-37° C dan pada suhu 4° C, 2-4 jam setelah kematian hewan (masing-masing 53; 66,66; dan 63%) (P0,05). Tingkat fertilisasi oosit mulai mengalami penurunan pada tiga kelompok perlakuan setelah ovarium disimpan selama 8-10 jam, tingkat fertilisasi oosit yang disimpan pada suhu 27-28° C; 36-37° C; dan suhu 4° C masing-masing berturut-turut sebesar 9,8; 22,22; dan 12,24%. Dari hasil penelitian disimpulkan bahwa penyimpanan ovarium pada suhu 27-28° C dan 36-37° C selama 5-7 jam dapat mempertahankan kompetensi oosit dibandingkan dengan penyimpanan pada suhu 4° C.

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Tongku Nizwan Siregar

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Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

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Home Page

OAI Link

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Contact Name Tongku Nizwan Siregar

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Editorial Address Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

Location Kab. aceh besar, Aceh INDONESIA

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Contact Name
Tongku Nizwan Siregar

Contact Email
jkh@usk.ac.id

Phone
+626517551536

Journal Mail Official
jkh@usk.ac.id

Editorial Address
Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

Location
Kab. aceh besar,

Aceh

INDONESIA