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Tongku Nizwan Siregar
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Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia
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INDONESIA
Jurnal Kedokteran Hewan
Published by Universitas Syiah Kuala
ISSN : 1978225X     EISSN : 25025600     DOI : 10.21157
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary
Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian Veterinary Medical Association (PDHI). Jurnal Kedokteran Hewan is a double-blind review process journal that has been accredited by National Journal Accreditation (ARJUNA), with second grade (Sinta 2), Number: 200 / M / KPTS / 2020. This journal has been registered in the Indonesian Publication Index (IPI), Google Scholar, Sinta, World Cat, Directory of Open Access Journals (DOAJ), EBSCO, Copernicus, Microsoft Academic, and other scientific databases. Jurnal Kedokteran Hewan receives scientific manuscripts in veterinary sciences (veterinary miscellaneous): anatomy, histology, physiology, pharmacology, parasitology, microbiology, epidemiology, veterinary public health, pathology, reproduction, clinic veterinary, aquatic animal disease, animal science, and biotechnology.
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 901 Documents
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INFLUENCE OF ZINGIBER ZERUMBET EXTRACTS AS FEED ADDITIVE ON PERFORMANCE, CARCASS CHARACTERISTICS AND INHIBITION OF ESCHERICHIA COLI BACTERIA OF COMMERCIAL BROILER CHICKENS Rafiqi, Rojaur; Ryanda, M. Afzalul; Fuadi, M. Afid; Riwa, Nanda; Wahyudi, Indra; Ilham, Ilham; Allaily, Allaily; Wajizah, Sitti; Samadi, Samadi
Jurnal Kedokteran Hewan Vol 16, No 2 (2022): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i2.25660

Abstract

The purpose of this study was to evaluate administration of Zingiber zerumbet (Z. zerumbet) extracts on performances, carcass characteristics and Echerichia coli (E. coli) bacteria of commercial broiler chickens. One hundred day old CP 707 broiler chickens (mixed sex) were randomly distributed into 20 floor pens with four treatments and five replicates. Each group of broilers was treated with diffrent level of Z. zerumbet extracts in drinking water (P0= control, P1= 0.025 % in drinking water, P2= 0.050 % in drinking water and P3= 0.075 % in drinking water). Broiler chickens were fed commercial diet based on nutrition requirement for starter and grower. Feed and water were provided ad libitum. Body wright of chickens and feed were recorded weekly. All data were recorded to measure for performances, carcass characteristics and the inhibition of E. coli bacteria. The data were analyzed by one-way ANOVA. Statistically differences among treatments were stated with a probablity of P0.05. The results of study indicated that administration of Z. zerumbet extract in drinking water with different concentration up to 4 weeks did not significantly influence (P0.05) on the performances, carcass and giblet characteristics of broiler chickens. The inhibition of E. coli bacteria was significantly affected (P0.05) by inclusion of Z. zerumbet at different concentration. In conclusion, administration of Z. zerumbet in drinking diet had no detrimental effect on animal performance, carcass and giblet characteristics.
Identification and Molecular Characterization of Newcastle Disease Virus Circulates in some districts in Aceh Daud AK, M; Setiyaningsih, Surachmi; Sudirman, Idwan
Jurnal Kedokteran Hewan Vol 13, No 1 (2019): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i1.5832

Abstract

The objectives of this study were to assess the diversity of Newcastle Disease Virus (NDV) isolates; to detect and isolate NDV from poultry; and to identify and characterize NDV by serological and molecular assays. A total of 84 cloacal-oropharynx isolates of poultry was collected from privately owned poultries and poultry markets from 12 districts in Aceh Besar and Banda Aceh. Screening was performed by real time reverse transcriptation-polymerase chain reaction (rRT-PCR) to 15 isolates of poultry. Selected isolates were inoculated in 9-11 days old embryonated specific pathogen free (SPF) eggs and showed positive hemagglutination (HA). Characterization was performed through hemagglutination inhibition (HI) test using Komarov and Hitchner B1 antisera, elution test, RT-PCR and realtime RT-PCR fusion (F). All isolates had a higher affinity to Komarov antisera (titer up to 4 log), indicating virulent strain. This was supported by elution test which showed that 93.66% isolates were virulent and 6 % non-virulent. In conclusion, RT-PCR can detect Matrix gene from 15 isolates (100%), while Fusion gene only detected from 11 isolates (73.3%). rRT-PCR is more capable of detecting antigenic diversity compared to RT-PCR.
ENDOPARASITES INFECTION IN BLOOD COCKLE (Anadara granosa) IN ACEH BESAR WATERS, INDONESIA Putra, Dedi Fazriansyah; Ramadina, Suci; Mellisa, Siska; Abdullah Abbas, Muhammadar; He he, Xiao
Jurnal Kedokteran Hewan Vol 15, No 3 (2021): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i3.20106

Abstract

This study aims to investigate the type, prevalence, and intensity of endoparasites infecting the organs of blood cockles (Anadara granosa) in the waters of Aceh Besar, Indonesia. A purposive sampling method was conducted from March to August 2020 consisting of two sampling sites, namely the waters of Ujung Pancu and Krueng Raya. Endoparasite identification and analysis was carried out at the Parasite Laboratory of the Fish Quarantine Center, Quality Control and Safety of Fishery Products in Blang Bintang, Aceh Besar. The results showed that there were two types of endoparasites living in the blood cockles: namely, Perkinsus sp., which belong to the group Protozoa, and Spiroxys sp. of the Nematode class. The prevalence levels of Perkinsus sp. was 73.33% and were found in the waters of Ujung Pancu; while the lowest prevalence level was 20% in blood cockles that were infected with Spiroxys sp., which were found both in Ujung Pancu and Krueng Raya. The highest intensity level was infections of Perkinsus sp. with a value of 89 ind/species in the Krueng Raya site. The lowest intensity value was infections of Spiroxys with a value of 2 ind/species in Krueng Raya and 2.3 ind/species in Ujung Pancu
THE POTENTIAL EFFECT OF RED DRAGON FRUIT (Hylocereus polyrhizus) PEEL ETHANOL EXTRACT ON ENDOMETRIOSIS PROGRESSIVITY IN ENDOMETRIOSIS MICE Hapsari, Anindya; Pujiastutik, Yanuar Eka
Jurnal Kedokteran Hewan Vol 12, No 2 (2018): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i2.10497

Abstract

The aim of this study was to know the effect of red dragon fruit (Hylocereus polyrhizus) peel ethanol extract at dose of 0.25, 0.5, and 1 mg/gbw on endometriosis progressivity, signed by granuloma, on mouse model of endometriosis. Twenty five female mice were used as samples anddivided into 5 groups: 1 positive control, 1 negative control, and 3 treatment groups. Positive control and treatment groups were induced as modelof endometriosis for 14 days. The next 14 days, placebo was given to both control groups, while red dragon fruit peel ethanol extract at dose of0.25, 0.5, and 1 mg/g bw were given to mice in treatment groups as much as 0.2 mL/25 g bw/day orally. Endometriotic lesion was examined.Result showed that there were significantly differences on granuloma among control groups and treatment groups. The conclusion was reddragon fruit peel ethanol extract could inhibit endometriosis progressivity, signed by granuloma, on mouse model of endometriosis.
IDENTIFIKASI PROTEIN EPIDERMAL GROWTH FACTOR (EGF) 46 kDa HASIL MATURASI OOSIT SAPI SECARA IN VITRO w, Widjiati; Rachmawati, Anike; Mumpuni, Sri; Sektiari, Bambang
Jurnal Kedokteran Hewan Vol 6, No 1 (2012): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i1.734

Abstract

Tujuan dari penelitian ini adalah mengidentifikasi suatu protein dengan berat molekul 46 kDa yang diduga sebagai epidermal growth factor(EGF) yang diisolasi dari oosit sapi yang telah dimaturasi secara in vitro dengan metode elektroforesis. Ovarium sapi yang berasal dari rumah potong hewan, diaspirasi pada folikel dengan diameter permukaan 5 mm menggunakan spuit dan jarum. Oosit dimaturasi dalam tissue culture medium (TCM) 199 selama 22 jam pada suhu 38,5 C di dalam inkubator CO2. Preparasi protein dengan berat molekul 46 kDa menggunakansodium dodecyl sulphate polyacrilamide gel elektroforesis (SDS PAGE). Berdasarkan perhitungan jumlah regresi dari protein marker, didapatkan 12 fraksi protein yaitu BM 172,7; 153,09; 118,24; 102,43; 89,27; 59,75; 46,41; 43,82; 40,2; 36,06; 23,45; dan 18,42 kDa. Protein dengan berat molekul 46,41 kDa yang tampak pada pita protein dapat diidentifikasi sebagai protein yang diduga EGF yang berperan dalam proses maturasi oosit.
DEKONTAMINASI BAKTERI Escherichia coli DAN Staphylococcus aureus PADA SARANG BURUNG WALET DENGAN PERLAKUAN PEMANASAN (Decontamination of Escherichia coli and Staphylococcus aureus in Edible Birds Nest Using Heat Treatment) Saimah, Saimah; B. Sudarwanto, Mirnawati; Latif, Hadri
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5045

Abstract

This research was aimed to examine the heating effect at 70 C for 3.5 seconds on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) decontamination in edible birds nest. This study used 40 clean edible birds nest samples. Samples were divided into two groups, first group was contaminated with E. coli and second group was contaminated with S. aureus. Each group was divided into two treatments. The first treatment was directly tested for microbiological examination and the second treatment was heating at temperature 70 C for 3.5 seconds prior to microbiological examination. The results showed that both of bacteria E. coli and S. aureus had been destroyed by heating treatment. Heating process at 70 C for 3.5 seconds was effective for decontamination of both E.coli and S.aureus.
AKTIVITAS SULFUR DAN SELENIUM NANOPARTIKEL TERHADAP CACING Steinerma feltiae DAN PERBANDINGAN TOKSISITASNYA TERHADAP SEL NEUROBLASTOMA (NEURO 2A CELL LINES) Khairan, Khairan; Idroes, Rinaldi; Bahi, Muhammad; Schaefer, Karl Herbert; Schneider, Thomas; Jacob, Claus
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2798

Abstract

Penelitian ini bertujuan mengetahui aktivitas sulfur dan selenium nanopartikel terhadap cacing Steinernema feltiae (S. feltiae) dan perbandingan toksisitasnya terhadap sel neuroblastoma (neuro 2A). Sulfur dan selenium nanopartikel aqueous nanoparticles dikarakterisasimenggunakan Nano Zetasizer pada pH 7 dan suhu 25 C. Dalam penelitian ini uji nematoda dilakukan terhadap S. feltiae. Hasil penelitian menunjukkan bahwa sulfur nanopartikel mempunyai aktivitas yang sangat tinggi terhadap S. feltiae dengan lethal dose 50 (LD50) berkisar pada 6,99 g/ml setelah 24 jam inkubasi. Sementara itu, live and dead assay dilakukan terhadap neuroblastoma sel (Neuro 2A cell lines). Hasil penelitian menujukkan bahwa aqueous sulfur nanopartikel (NPS) menunjukkan aktivitas yang lebih baik dibandingkan dengan aqueous selenium nanopartikel (NPSe) terhadap sel neuroblastoma (neuro 2A cell lines) dengan IC50 1 g/ml.
KAJIAN DIVERSITI GENETIKA Tarsius sp. ASAL INDONESIA MENURUT URUTAN GEN NADH DEHIDROGENASE SUBUNIT 4 (ND4) H, Herrialfian; Widayanti, Rini; Wijayanto, Hery; J, Jalaluddin
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i1.1247

Abstract

Penelitian ini bertujuan mengkaji keragaman genetik gen penyandi ND4 pada Tarsius bancanus, T. b. borneanus, T. dianae dan T. spectrum dan untuk penegakan taksonominya. Deoxyribonucleic acid (DNA) diisolasi dari biopsi jaringan masing-masing spesies Tarsius dengan cara diekstraksi untuk digunakan sebagai DNA cetakan dalam proses amplifikasi dengan metode polymerase chain reaction (PCR). Primer yang digunakan dalam penelitian ini didesain untuk mengamplifikasi gen ND4 dan dilanjutkan dengan elektroforesis. Produk PCR hasil amplifikasi yang telah dimurnikan, selanjutnya dipergunakan sebagai DNA cetakan untuk reaksi penentuan runutan nukleotida. Runutan nukleotida gen ND4 hasil pengurutan dilakukan penjajaran berganda dengan primata lain yang diambil dari Genbank menggunakan Clustal W. Selain berdasarkan runutan nukleotida, gen ND4 dianalisis berdasarkan runutan asam amino dari basa-basa yang diterjemahkan mengikuti vertebrate mitochondrial translation code yang ada pada program MEGA versi 4.1. Konstruksi pohon filogenetika menggunakan metode neighbor joining. Hasil penelitian menunjukkan dari 1378 nukleotida ditemukan 119 situs yang bersifat beragam. Jarak genetika berdasarkan nukleotida gen ND4 yang dihitung menggunakan model dua parameter Kimura, terdapat nilai paling kecil 0,6%, nilai terbesar 13%, dan nilai rata-rata sebesar 6,1%. Filogram berdasarkan hasil runutan nukleotida gen ND4 yang menggunakan metode neighbor joining, dapat mengidentifikasi dan membedakan percabangan antar spesies Tarsius.
COFFEE CONSUMPTION MAY DECREASE THE SERUM CREATININE AND PLASMA MALONDIALDEHYDE LEVELS IN RATS WITH HYPERURICEMIA INDUCED BY A HIGH PURINE DIET Sudiarto, Hilmi Ardian; Yuantari, Rahma; Nur Ahsani, Dwi
Jurnal Kedokteran Hewan Vol 15, No 2 (2021): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i2.19141

Abstract

The aim of this research was to determine the effect of coffee on creatinine and plasma malondialdehyde (MDA) level in rats fed on high purine diet. A total of 24 male Wistar rats divided into 4 groups of 6 rats each. Beef broth were given to 3 groups (K1, P1, P2) for thirty days. Beef broth administration were followed by giving coffee (caffeinated= P1 or by a decaffeinated= P2, 144 mg/200 g BW). Serum creatinine levels and plasma MDA were examined periodically on day 0, 15, and 30. Data were analyzed by multivariate analysis of variance or MANOVA test (CI= 95%, P0.05). Coffee administration (caffeinated and decaffeinated) may decrease the serum creatinine (day-30, K1= 3.17+ 0.69; P1= 1.63+0.11; P2= 1.14+0.08) and MDA levels (day-30, K1= 9.8+0.28; P1= 5.80+0.55, P2= 3.87+3.26) after 30 days of treatment (P= 0.000 for creatinine and MDA levels in K1, P1, P2 groups). A lower serum creatinine and MDA levels can be found in decaffeinated coffee grup. Coffee consumption for 30 days (especially decaffeinated) may decrease serum creatinine and plasma MDA in rat induced by a high purine diet.
STEROID LEVEL AND PREGNANCY RATE OF ACEH COWS IN RESPONSE TO OVULATION INDUCTION USING PRESYNCHOVSYNCH METHOD Adam, Mulyadi; Siregar, Tongku Nizwan; Wahyuni, Sri; Gholib, Gholib; Ramadhana, Cut Erika; Ananda, Riski; Afifuddin, Afifuddin
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.8994

Abstract

Penelitian ini bertujuan mengetahui peningkatan level steroid dan persentase kebuntingan sapi aceh terhadap induksi ovulasi dengan metode presynch-ovsynch. Dalam penelitian ini digunakan sepuluh ekor sapi aceh betina dengan status tidak bunting, minimal dua bulan pascapartus, sudah pernah beranak, dan sehat secara klinis. Sapi dibagi atas dua kelompok, yang masing-masing terdiri atas lima ekor sapi. Kelompok pertama (K1) disinkronisasi berahi dengan metode presynch-ovsynch. Pada kelompok kedua (K2), disinkronisasi berahi menggunakan 5 ml PGF2 secara intramuskulus dengan pola penyuntikan ganda dengan interval 12 hari. Setelah 48 jam akhir perlakuan, sapi pada K1 dan K2 diinseminasi menggunakan semen beku fertil. Observasi berahi dilakukan setelah penyuntikan terakhir. Koleksi darah untuk pemeriksaan level estradiol dilakukan segera setelah inseminasi dilakukan sedangkan koleksi darah untuk pemeriksaan progesteron dilakukan pada hari ke-7 pasca-inseminasi. Level steroid diukur menggunakan teknik enzyme-linked immunosorbent assay (ELISA). Pemeriksaan kebuntingan dilakukan 90 hari pasca-inseminasi menggunakan teknik palpasi rektal. Seluruh sapi menunjukkan gejala berahi setelah perlakuan. Level estradiol dan progesteron pada K1 vs K2 masing-masing adalah 294,98110,48 vs 392,7611,6 pg/ml (P0,05) dan 23,8515,14vs 12,695,64ng/ml (P0,05). Persentase kebuntingan pada K1 vs K2 masing-masing adalah 60,0 vs 0,0%. Dari hasil penelitian disimpulkan bahwa metode presynch-ovsynch tidak dapat meningkatkan level steroid tetapi dapat meningkatkan persentase kebuntingan pada sapi aceh.

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