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INDONESIA
Jurnal Kedokteran Hewan
Published by Universitas Syiah Kuala
ISSN : 1978225X     EISSN : 25025600     DOI : 10.21157
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary
Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian Veterinary Medical Association (PDHI). Jurnal Kedokteran Hewan is a double-blind review process journal that has been accredited by National Journal Accreditation (ARJUNA), with second grade (Sinta 2), Number: 200 / M / KPTS / 2020. This journal has been registered in the Indonesian Publication Index (IPI), Google Scholar, Sinta, World Cat, Directory of Open Access Journals (DOAJ), EBSCO, Copernicus, Microsoft Academic, and other scientific databases. Jurnal Kedokteran Hewan receives scientific manuscripts in veterinary sciences (veterinary miscellaneous): anatomy, histology, physiology, pharmacology, parasitology, microbiology, epidemiology, veterinary public health, pathology, reproduction, clinic veterinary, aquatic animal disease, animal science, and biotechnology.
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 901 Documents
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THE EFFECT OF Moringa oleifera LEAF EXTRACT ON THECA CELL IN POLYCYSTIC OVARY SYNDROME MODEL WITH INSULIN RESISTANCE Wulandari, Lisa Purbawaning; Hapsari, Anindya
Jurnal Kedokteran Hewan Vol 14, No 3 (2020): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i3.16919

Abstract

The use of Moringa oleifera as an anti-oxidant should be investigated as an alternative treatment of follicular refinement in Polycystic ovary syndrome (PCOS) with insulin resistance. We aimed to prove the effect of Moringa oleifera leaf extract in various dosages to decrease the thecacell thickness of PCOS female rat with insulin resistance. This study was a laboratory experimental research. Three month old Rattus norvegicusstrain Wistar rat weighing 100-130 g were divided into 5 groups (n= 8). PCOS model obtained by giving injection of testosterone propionate for28 days, followed by metformin therapy and Moringa oleifera leaf extract at 250 and 500 mg/kg BW for 14 days. The examination of ovariumhistology showed that leaf extract Moringa oleifera 500 mg/kg BW (0.9310.457) significantly decreased the thickness of theca cells (P0.05)compared to the PCOS control group. The conclusion was Moringa oleifera leaf extract as an anti-oxidant proven to decrease the thickness oftheca cell of the female rat model of PCOS.
Antimicrobial Activity and LC Determination of Ethanol Crude Extract of 50 Pliek U, an Achehnese Traditional Fermented Food Nurliana, Nurliana; Sudarwanto, Mirnawati; Sudirman, Lisdar I.; W. Sanjaya, Angelina
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i1.9795

Abstract

Antimicrobial activity of pliek u crude extracts was evaluated against five bacterial species (Staphylococcus aureus, Bacillus cereus, Escherichia coli, Salmonella enterica serovar Enteritidis and Pseudomonas aeruginosa,)and one fungal species (Candida albicans). Pliek u has been consumed as spices and hot sauce and poultry feed. These foods were collected from household industry at Reudep village in Aceh Besar, Province of Aceh. The ethanol extract of pliek u were obtained by standard method. The antimicrobial activity was detected using paper disc method. The concentration of ethanol crude extract of pliek u (EEP) was determined with the dilution method. The lethality initial test has been detected by using Artemia salina L. bioassay to determine the toxic concentration based on the LC value of EEP. The ethanol crude extract (EEP) was active against all microbial strains. EEP showed 50 antimicrobial activity at a minimum inhibitory concentration (MIC) and a minimum microbicidal concentration (MMC) at 2.5-10 mg/ml and 10-20 mg/ml, respectively. The lethality concentration EEP gave the LC value of 3.36 50 mg/ml. It was concluded that ethanol crude extract (EEP) shows significant antimicrobial activity and it is not toxic.
SINKRONISASI ESTRUS DAN PENGAMATAN ULTRASONOGRAFI PEMERIKSAAN KEBUNTINGAN DINI PADA DOMBA GARUT (Ovis aries) SEBAGAI STANDAR PENENTUAN UMUR KEBUNTINGAN a, Amrozi; Setiawan, Bagus
Jurnal Kedokteran Hewan Vol 5, No 2 (2011): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v5i2.364

Abstract

Penelitian ini dilakukan untuk mengamati sinkronisasi estrus dan menentukan diagnosis kebuntingan dini pada domba garut (n=3) menggunakan ultrasonografi transrektal. Domba disinkronisasi dengan menggunakan PGF pada fase luteal. Onset dan durasi estrus diamati dengan menggunakan jantan pengusik. Kebuntingan 2a ditentukan dengan tampilan isoechogenic yang dikelilingi oleh tampilan hypoechogenic. Rata-rata onset estrus adalah 3528,7 jam dan rata-rata lama estrus adalah 3313,6 jam. Kebuntingan dini terdeteksi pada hari ke-22 (22,30,6 hari). Perkembangan fetus diikuti dengan peningkatan diameter dan ketebalan uterus. Diameter uterus meningkat dari hari ke-22 (1,80,7 cm) hingga hari ke-42 (5,61,1 cm), dan tebal uterus meningkat dari hari ke-22 (0,80,1 cm) hingga hari ke-42 (2,10,5 cm). Plasentom muncul pada kebuntingan hari ke-34 (0,80,2 cm) dan menunjukkan pola perkembangan yang terus meningkat secara signifikan sampai hari ke-56 (2,70,5 cm; P0,05). Diameter kotiledon hari ke-34 sekitar 0,80,2 cm hingga hari ke-56 (2,70,5 cm) dan hari ke-77 (3,30,4 cm). Dapat disimpulkan bahwa diagnosis positif dari kebuntingan pada domba garut terlihat pada hari ke-12 dan fetus dapat diamati pada hari ke-22.
POTENSI TRANSDIFERENSIASI SEL FIBROBLAS MENJADI SEL SARAF SECARA IN VITRO (Transdifferentiation Potency of Fibroblast Cell to Neuron Cell in Vitro) Kaiin, Ekayanti M.; Djuwita, Ita
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i1.3370

Abstract

The aim of this study was to examine the potency of fibroblast cells transdifferentiated to neuron cells in vitro. Newborn rat neuron conditioned medium (NBRN CM) was collected from neuron cells cultured with mDMEM without serum for 48 hours. Fibroblast cells were collected from fetal rat muscle treated with trypsin. Fibroblast cells were culture with 3 kind of culture medium: mDMEM + 0.01 mM -mercaptoethanol; mDMEM + 50% NBRN-CM and mDMEM + 0.01 mM -mercaptoethanol + 50% NBRN CM . As control, cells was cultured with mDMEM +10% newborn calf serum (NBCS). The addition of NBRN CM into culture medium resulted in 12.97% newborn cells in fibroblast culture medium passage I. Newborn rat neuron conditioned medium in fibroblast culture medium resulted 12.97% neuron cells at passage 1. The percentage was increased (14.60%) when - mercaptoethanol added into medium. The same result was found at passage 3 (12.67%; 13.17%). It showed that fibroblast cells has potency to transdifferentiated into neuron cells when cultured with NBRN CM. Further research is needed to know the fibroblast transdifferentiation potency.Key words: fibroblast, transdifferentiation, conditioned medium, neuron cells, in vitro
KAJIAN KADAR KORTISOL SAPI YANG DIPOTONG DI RUMAH POTONG HEWAN YOGYAKARTA S, Sarmin; Hana, Amelia; Heru Fibrianto, Yuda; Mona Airin, C.
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i2.2635

Abstract

Penelitian ini bertujuan mengkaji tingkat stres sapi yang dipotong di Rumah Potong Hewan (RPH) Yogyakarta. Dalam penelitian ini digunakan 20 ekor sapi yang dibagi menjadi dua kelompok, yakni kelompok sapi yang dilakukan restraint dengan restraining box tipe Mark 1 (K1, n=13) dan kelompok sapi yang dilakukan restraint dengan metode konvensional (K2, n= 7). Masing-masing kelompok diambil darahnya sebelum dan saat dipotong untuk diperiksa kadar kortisol dengan enzyme-linked immunosorbent assay (ELISA). Rata-rata kortisol sapi betina sebelum vs setelah restraint pada K1 dan K2 masing-masing adalah 55,6452,13 vs 21,2320,63 ng/ml dan 127,48150,19 vs 106,28+75,39 ng/ml. Rata-rata kortisol sapi jantan sebelum vs setelah restraint pada K1 dan K2 masing-masing adalah 40,2838,12 vs 24,2117,21 ng/ml dan 49,5138,67 vs 69,6263,98 ng/ml. Rata-rata kadar kortisol tanpa memperhatikan jenis kelamin pada K1 dan K2 masing-masing adalah 79,5088,50 vs 95,8069,69 ng/ml (P>0,05). Disimpulkan bahwa tidak terdapat perbedaan kadar kortisol sebagai indikator stres pada sapi-sapi yang dipotong di RPH Yogyakarta yang mengalami restraint dengan restraining box tipe Mark 1 dan restraint metode konvensional.
THE INFLUENCE OF NUTRITIONAL FLUSHING ON BODY MORPHOMETRICS OF FEMALE BREEDING GOATS Isnaini, Nurul; Amertaningtyas, Dedes; Sulistyo, Hanief Eko; Irsyammawati, Artharini; Andri, Faizal
Jurnal Kedokteran Hewan Vol 16, No 2 (2022): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i2.23464

Abstract

The purpose of this current study was to evaluate the influence of nutritional flushing on body morphometrics of female breeding goats. This study used 32 local female goats or does with an average age of 3.281.08 years and an average body weight of 42.478.28 kg. The goats were distributed in a completely randomized design using a 2 x 2 factorial pattern with 8 replications. The first factor was the flushing feed (FF) type, consisting of rice bran and concentrate, whereas the second factor was the level of tannin-protected arginine (TPA: 0 and 300 mg/kg). The body morphometrics observed in this study were initial body length (IBL), final body length (FBL), body length change (BLC), initial chest girth (ICG), final chest girth (FCG), chest girth change (CGC), initial wither height (IWH), final wither height (FWH), and wither height change (WHC). The IBL, FBL, BLC, ICG, FCG, IWH, FWH, and WHC of the local does were not significantly different (P0.05) after receiving the FF type. However, FF significantly affected the CGC of the goats (P0.001). The use of concentrate for the local female local goats resulted in a higher CGC (P0.001) compared to the use of rice bran. On the other hand, TPA did not significantly affect all body morphometrics of the goats (P0.05). Thus, it can be concluded that the use of concentrate leads to higher chest girth change, giving better body growth compared to the use of rice bran. The addition of tannin-protected arginine was also found to have no significant contribution to the body morphometrics of the local female goats. _____________
ANALYSIS OF APOLIPOPROTEIN-B (APO-B) GENE IN ATHEROSCLEROSIS MICE GIVEN CURCUMINOID EXTRACT OF ZANTHORRIZA IN ORAL Susmiati, Trini; Widayanti, Rini; Purwantoro, Aris; Airin, Claude Mona; Sarmin, Sarmin
Jurnal Kedokteran Hewan Vol 13, No 2 (2019): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i2.10219

Abstract

The aim of this study was to investigate the apolipoprotein-B (apo-B) gene in atherosclerosis mice which were orally given curcuminoid extract of Curcuma xanthorriza. A total number of 30 white mice were split into 6 groups, the first group considered as control (without any treatment), second group as atherogenic feed control, the third group as extract control, while the fourth, fifth and sixth groups as atherogenic feed and curcuminoid Curcuma xanthorriza extract group treated with 5 mg/mouse, 10 mg/mouse and 15 mg/ mouse, respectively for three months. The blood samples were taken from all six groups for the deoxyribonucleic acid (DNA) analysis using total DNA isolation, DNA amplification with polymerase chain reaction (PCR), and DNA sequencing. The data analysis showed that 374 bp nucleotide sequence gen of apo-B from Rattus norvegicus in groups B, C, D, E, and F did not cause any changes in genes. The analysis showed the sequence of apo-B Rattus norvegicus gene in the treatment group was apparently identical with that of Rattus norvegicus group A as the control group without treatment. As conclusion, the administration of curcuminoid zanthorrizza to atherosclerosis mice did not change the gene structure of apo-B 100.
FREEZING CAPABILITY OF PASUNDAN BULL SPERM USING TRIS-EGG YOLK, TRIS-SOY, AND ANDROMED DILUENTS Baharum, Abdullah; Arifiantini, R. Iis; Yusuf, Tuty Laswardi
Jurnal Kedokteran Hewan Vol 11, No 1 (2017): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i1.5810

Abstract

The aims of this study were to investigate the freezing capability of Pasundan bull spermatozoa in Tris-egg yolk (TEY), Tris-soy (TS), and AndroMed as diluents. Semen were collected twice a week from four Pasundan bulls aged 3-5 years old using an artificial vagina and evaluated macro- and microscopically. Semen had 70% sperm motility, 800x106/mL sperm concentration, and less than 20% sperm abnormalities were divided into three parts and each of them diluted with TEY, TS, or AndroMed. After an equilibration step at 5C for four hours, diluted semen were packaged in 0.25 mL straw, frozen in liquid nitrogen for ten minutes and kept in liquid nitrogen container until examination. Motility test on fresh, diluted, equilibrated, and after-thawed semen was done using Androvision. The results showed that after thawing motility of sperm diluted in AndroMed (58.640.72%) was higher than in TEY (49.451.22%) and TS (39.346.33%). Sperm motility of Pasundan bulls diluted in these three diluents reduced around 33.272.45% during freezing process.
KONSENTRASI PROTEIN DAN PENENTUAN BERAT MOLEKUL EKSKRETORI/SEKRETORI L3 Ascaridia galli D, Darmawi; Balqis, Ummu; Tiuria, Risa; Soejoedono, Retno D; Pasaribu, Fachriyan H
Jurnal Kedokteran Hewan Vol 3, No 1 (2009): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v3i1.3074

Abstract

Penelitian ini bertujuan menentukan konsentrasi dan berat molekul protein ekskretori/sekretori larva (L3) Ascaridia galli (A. galli). Larva L3 diperoleh dari usus halus 100 ayam tujuh hari setelah pemberian dosis 6000 L2 melalui esofagus ayam. Sebanyak 510 L3 dikultur secara in vitro dalam setiap ml medium Rosswell Park Memorial Institute (RPMI 1640), pH 6,8, tanpa merah fenol dalam inkubator pada temperatur 37 0C dan 5% CO2 selama 3 hari. Ke dalam medium ditambahkan 100 unit ml-1 penisilin G, 100 g ml-1 streptomisin, 5 g ml-1 gentamisin dan 0,25 g ml-1 kanamisin. Ekskretori/sekretori dipreparasi dari produk metabolisme L3 yang dilepaskan ke dalam medium kultur. Untuk mendapatkan protein ekskretori/sekretori, medium kultur dipekatkan dengan vivaspin 30.000 MWCO, dan kuantitas protein dihitung dengan metode Bradford. Berat molekul protein ekskretori/sekretori divisualisasikan dengan sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE). Hasil penelitian menunjukkan bahwa konsentrasi protein ekskretori/sekretori adalah 0,595 mg/ml dengan berat molekul 28 kDa.
FEEDING LOCAL FERMENTED WITH EM-4, AMMONIUM SULFATE, AND UREA ON THE BLOOD FAT LEVEL OF MALE DUCK Septinova, Dian; Arti, Aisyah Yuli; Sutrisna, Rudy; Tantalo, Syahrio; Hartono, Madi
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i2.16501

Abstract

The objective of this study was to determine the effect of commercial ration by the addition of ammonium sulfate and urea fermented feed ingredients on the blood fat levels of local male ducks. This study used a completely randomized design with 32 male ducks which weredivided into four groups namely R0, R1, R2, R3, and R4. Each group was given the following treatment: R0 only given commercial ration;R1 was given 80% commercial ration + 20% fermentation of EM-4 + 1.5% urea); R2 was given 80% commercial ration + 20% EM-4 + 1.5%ammonium sulfate fermentation; and R3 was given 80% commercial ration + 20% EM-4 + 1.5% urea + 1.5% ammonium sulfate. Variablesin this study were blood fat consisting of cholesterol, triglyceride, High-Density Lipoprotein (HDL), and Low-Density Lipoproteins (LDL).The data obtained were analyzed by using 5% real level and LSD. The results showed that the provision of commercial ration added withlocal feed fermented with ammonium sulfate and urea had a significant effect on cholesterol levels (P0.5), but not significant (P0.05) ontriglyceride, HDL, and LDL levels. Furthermore, the ration that gave the best effect on the blood fat levels of male ducks blood was the ration given to R3 group.

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