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Tongku Nizwan Siregar
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INDONESIA
Jurnal Kedokteran Hewan
Published by Universitas Syiah Kuala
ISSN : 1978225X     EISSN : 25025600     DOI : 10.21157
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary
Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian Veterinary Medical Association (PDHI). Jurnal Kedokteran Hewan is a double-blind review process journal that has been accredited by National Journal Accreditation (ARJUNA), with second grade (Sinta 2), Number: 200 / M / KPTS / 2020. This journal has been registered in the Indonesian Publication Index (IPI), Google Scholar, Sinta, World Cat, Directory of Open Access Journals (DOAJ), EBSCO, Copernicus, Microsoft Academic, and other scientific databases. Jurnal Kedokteran Hewan receives scientific manuscripts in veterinary sciences (veterinary miscellaneous): anatomy, histology, physiology, pharmacology, parasitology, microbiology, epidemiology, veterinary public health, pathology, reproduction, clinic veterinary, aquatic animal disease, animal science, and biotechnology.
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 901 Documents
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PHYLOGENETIC ANALYSIS AND VIRULENCE-CODING GENES CHARACTERIZATION OF NEWCASTLE DISEASE VIRUS ISOLATED FROM LAYING HEN BRAIN WITH TETELO SYMPTOM Sarah, Safira Iqlima; Nugroho, Christian Marco Hadi; Kurnia, Ryan Septa; Wardhana, Dhandy Koesoemo; Puspitasari, Heni
Jurnal Kedokteran Hewan Vol 13, No 3 (2019): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i3.14493

Abstract

Newcastle disease (ND) is an acute poultry disease caused by Paramyxovirus group. It has characteristic neurological symptoms, called torticollis. The molecular assay to find out the presence of viral genes in the brain can be an option in detecting ND virus infections since it penetrates brain barrier system. The purpose of this study was to identify ND viruses in the brain of chickens with torticollis symptoms, to analyze its phylogenetic and to characterize its virulence genetic code. Samples used were 12 dead chickens with historically had torticollis symptoms, obtained from poultry farms at several areas in West Java and Banten. Chicken brains were prepared for reverse transcriptasepolymerase chain reaction (RT-PCR) test. All positive samples then sequenced to obtain its nucleotide sequences from some of Fusion (F) genes analyzed its phylogenetic by comparing with Indonesian ND isolate virus from GenBank using Mega X software. The results of RT-PCR test showed that only one sample (Virus MSL.03) contained genes of ND virus. Based on homology tests and phylogenetic analysis, the virus belonged to subgenotype VIIh with an identical level of 95.34-95.86% when compared to several isolates from Indonesia. The MSL.03 ND virus has 112RRRKRF117 pattern in F0 indicatest its virulent category.
PECTORALIS THORACICUS MUSCLE PERFORMANCE OF HYBRID CHICKEN (F1) DERIVED FROM CROSSBREED BETWEEN BROILER AND PELUNG (Gallus gallus gallus) Saragih, Hendry Tri Sakti; Roosdianto, Iwan; Daryono, Budi Setiadi
Jurnal Kedokteran Hewan Vol 11, No 2 (2017): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i2.2282

Abstract

This research purpose was to study the phenotype characters of body weight and pectoralis thoracicus muscle performance of hybrid chicken (F1) derived from crossbreed between parent stock broiler strain Cobb-500 and Pelung (Gallus gallus gallus). The chicks were divided into 3 groups and each group consists of 5 days-old chicks (DOC). Group 1 (T1) was used as control involving DOC derived from crossbreed between female and male of broiler Cobb-500 strain. Group 2 (T2) involving DOC derived from crossbreed between female and male Pelung, while Group 3 (T3) was DOC derived from crossbreed between female broiler strain Cobb-500 and male Pelung. The chicken was grown up to 49 days to evaluate the body weight, the pectoralis thoracicus weight, the pectoralis thoracicus area, and myofiber area. Data were statistically analyzed using one way Anova. The results showed that the average of body weight in T1; T2; and T3 were 2,17086.02; 506.220.02; and 1,23868.25 g, respectively (P0.05). The average of pectoralis thoracicus muscle weight in T1; T2; and T3 were 165.0411.97; 21.262.44; and 68.741.94 g, respectively (P0.05). The average of pectoralis thoracicus muscle area in T1; T2; and T3 were 97.09,7; 616,4; and 63,41,8 cm2 , respectively (P0,05). The average of pectoralis thoracicus myofiber area in T1; T2; and T3 were 14.721.04; 4.662.1; and 13.131.3 m2 , respectively (P0.05). It was concluded that crossbreed between broiler strain Cobb-500 and Pelung improved the body weight and pectoralis thoracicus muscle performance of hybrid chicken (F1).
EFEK BUBUK TEMPE INSTAN TERHADAP KADAR MALONALDEHID (MDA) SERUM TIKUS HIPERGLIKEMIK Desminarti, Susi; r, Rimbawan; Anwar, Faisal; Winarto, Adi
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.294

Abstract

Penelitian ini bertujuan mengevaluasi pengaruh bubuk tempe instan terhadap kadar malonaldehid (MDA) pada serum tikus hiperglikemik. Sebanyak 25 tikus jantan Spraque Dawley umur dua bulan digunakan dalam penelitian ini. Tikus dibagi atas lima kelompok perlakuan yaitu A (normal + diet standar), B (hiperglikemik + diet standar), C (hiperglikemik + 20% bubuk tempe segar), D (hiperglikemik + 20% bubuk tempe instan), dan E (hiperglikemik + 35% bubuk tempe instan). Tikus dikondisikan menjadi hiperglikemik dengan induksi streptozotocin dosis tunggal 45 mg/kg bb intraperitoneum. Diet tempe diberikan selama 8 minggu. Hasil penelitian memperlihatkan bahwa rata-rata kadar MDA kelompok A; B; C; D; dan E masing-masing adalah 2,16; 3,67; 3,13; 3,08; dan 2,98. Substitusi 35% bubuk tempe instan pada diet standar dapat menurunkan kadar MDA serum tikus hiperglikemik yang paling tinggi.
Effect of Heat Stress on Body Weight Gain, Heterophile-Lymphocite Ratio and Body Temperature in Broiler S, Sugito; Delima, Mira
Jurnal Kedokteran Hewan Vol 3, No 1 (2009): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v3i1.3082

Abstract

Increasing in ambient temperature inside the cage could lead to heat stress in broilers. This research was conducted to find out effects of heat stress on body weight gain, heterophile-lymphocite ratio and body temperature in chicken broiler. Twenty broilers aged 20 days (strain Cobb) were randomly divided into 2 groups. The first group was treated with no heat stress, the second one was caged in 331 0C temperature for 4 hours per day for 14 days. The results indicated that heat stress reduced body weight gain, increased body temperature, and changed behavior, but no effect on feed conversion ratio (FCR) and heterophile-lymphocyte ratio. It suggested that the heat stress caused detrimental effects on broiler chicken.
GAMBARAN HISTOPATOLOGIS DAN KLINIS AYAM HERBAL SETELAH DIUJI TANTANG DENGAN VIRUS AVIAN INFLUENZA H5N1 Setiyono, Agus; Bermawie, Nurliani
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i1.1252

Abstract

Tujuan penelitian ini adalah mengetahui perubahan histopatologis dan klinis ayam herbal setelah ditantang dengan virus avian influenza (AI) H5N1. Seluruh ayam dibagi atas 3 kelompok perlakuan Kelompok kontrol, Kelompok I (I-1, I-2, dan I-3), dan Kelompok II (II-1, II-2, dan II-3). Masing-masing kelompok terdiri atas 15 ekor ayam sehingga jumlah seluruh ayam yang digunakan adalah 105 ekor. Ayam Kelompok I dan II masing-masing diberi herbal I (sambiloto, temu ireng, adas bintang, sirih merah) dan herbal II (sambiloto, adas bintang, sirih merah) selama 3 minggu sebelum ditantang virus. Ayam kelompok perlakuan secara keseluruhan tidak ada yang hidup hingga hari ke-8. Hasil penelitian menunjukkan bahwa 7 ekor ayam KII-3 (46,7%) masih hidup sampai hari ke-4 setelah uji tantang virus. Perubahan histopatologis sistem pernafasan ayam kelompok perlakuan menunjukkan pembendungan, edema, dan kerusakan sel epitel mukosa. Sistem limfoid juga menunjukkan pembendungan, deplesi folikel limfoid, dan fibrosis limpa dan bursa Fabrisius. Analisis imunohistokimia mengindikasikan partikel virus AI telah menyebar di organ atau jaringan sistem pernafasan dan sistem pertahanan.
THE EFFECT OF AEROB AND ANAEROBIC CONDITION AND ADDITION OF CaCO3 ON THE GROWTH AND ANTIBACTERIAL ACTIVITY OF LACTIC ACID BACTERIA ISOLATED FROM LAYER HENS INTESTINE AFTER ADMINISTRATED OF AKBISprob Nurliana, Nurliana; Khairunisa, Fauzia; Musni, Nailah; Vanda, Henni; Rasmaidar, Rasmaidar; Hasan, Muhammad; Asmilia, Nuzul; Sari, Wahyu Eka; Isa, Muhammad
Jurnal Kedokteran Hewan Vol 17, No 3 (2023): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i3.20309

Abstract

This study aimed to determine the effect of aerobic and anaerobic incubation, and the addition of CaCO on the growth and antibacterial activity of Lactic Acid Bacteria (LAB) isolated from laying hens intestines (strain ISA Brown) after given 4% AKBISprob. The LAB used in this study was the isolates strains that produced the strongest antibacterial activity with inhibition zone 5 mm. The treatments consisted of two incubations condition (aerobic and anaerobic) with five concentrations of CaCO3 (1%, 2%, 3%, 4%, and 5%). Each treatment consisted of three repetitions. Total Plate Count (TPC) method and the agar diffusion method were used to calculate the amount of LAB and antibacterial activity, respectively. The results showed that anaerobic conditions had a significant effect (P0.05) on the increase of LAB growth, but both of aerobic and anaerobic conditions (P0.05) did not show significant effect on antibacterial activity. The addition of CaCO3 had a significant effect (P0.05) on the increase in growth and antibacterial activity of LAB. Based on the results of the study, it can be concluded that anaerobic incubation can increase the growth of two isolate of LAB isolates (P31S72 and P31H62), however aerobic and anaerobic incubation have no effect on antibacterial activity. In addition, the administration of 3% CaCO3 can increase the growth and antibacterial activity of all LAB isolates.
IDENTIFICATION OF REPRODUCTIVE DISORDERS IN FEMALE CATTLE AT LOCAL FARMS IN WOHA SUB-DISTRICT, BIMA REGENCY HY, Lukman; khoirani, kuntum; Karni, Ine
Jurnal Kedokteran Hewan Vol 17, No 3 (2023): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i3.26553

Abstract

This research aims to identify reproductife disorders in female cattle raised by farmers in Woha Sub-district, Bima Regency, West Nusa Tenggara Province. The study involves the identification of 141 female cattle (56 heifers and 85 cows). The research is carried out by bringing the cattle to the designated locations determined by the farmers group leader, which includes fields and barns. Data collection is conducted through rectal examination of the reproductive system and analysis of records from farmers and officials. The research method includes several stages, namely anamnesis, clinical examination, and rectal examination of the reproductive system. The obtained data is then recorded and analyzed descriptively. Based on the examination results, it is known that 210 female cattle experienced reproductive disorders, including silent heat (4.55%), vaginitis (21.8%), follicular cysts (12.73%), persistent corpus luteum (11.82%), ovarian hypofunction (5.45%), and endometritis (20.91%) of cows and silent heat (8%), vaginitis (28%), follicular cysts (12.73 %) corpus luteum persisten (15 %), and ovarian hypofunction (5%) of heifers.
THE GENE EXPRESSION OF ADAM17 AS A GENETIC MARKER OF ALZHEIMER DISEASES IN THE BRAIN OF LONG-TAILED MACAQUES (Macaca fascicularis) Rosmanah, Lis; Hasya, Karin katina; Saepuloh, Uus; Manalu, Wasmen; Winarto, Adi; Sudarnika, Etih; Darusman, Huda Shalahudin
Jurnal Kedokteran Hewan Vol 17, No 3 (2023): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i3.31014

Abstract

This study aims to identify the expression of the a disintegrin and metalloproteinase 17 (ADAM17) gene as a marker of Alzheimer's disease in long-tailed macaques (Macaca fascicularis). This study used six brain samples (hippocampus and cortex regions) of long-tailed macaques which was divided into two groups consisting of aged long-tailed macaques and adult long-tailed macaques. The expression of ADAM17 gene was determined by comparing the relative quantification values between the two age groups, and brain regions consisting of the hippocampus and cortex regions. The results of data analysis showed no significant difference in the expression of ADAM17 gene between brain regions and between age groups of long-tailed macaques. However, numerically the results showed a lower expression of ADAM17 gene in the hippocampus region of aged macaques. Lower expression of ADAM17 gene could be a marker of old animals indicating the stages of Alzheimer's disease.
MOLECULAR DETECTION OF Coxiella burnetii THE CAUSE OF ZOONOSIS Q FEVER IN VARIOUS ORGANS OF CATTLE IN BANDUNG REGENCY Purnamiharja, Tsulatsy Puspitasari; Juniantito, Vetnizah; WoroWiranti, Rahayu; Setiyono, Agus
Jurnal Kedokteran Hewan Vol 17, No 3 (2023): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i3.32716

Abstract

Primary objective of this study was to ascertain the presence of Coxiella burnetii (C. burnetii) as the causative of Q fever in cattle within Bandung Regency. Tissue samples were procured from 240 slaughtered cattles in abattoirs across Bandung Regency. Each breed of cattle consists of Brahman cross (BX), Simmental, Limousin, Friesien Holstein crossbreed, and Friesien Holstein (FH) with total of 192, 3, 13, 29, and 3, respectively. Detection method employed was nested-Polymerase Chain Reaction (PCR) to identify the presence of C. burnetii Deoxyribonucleic Acid (DNA) within tissue samples. Positive samples underwent further examination employing hematoxylin-eosin (HE) staining to identify lesions attributed to C. burnetii. Five BX cattle were identified as positive for C. burnetii in lungs and heart. However, histopathological analysis of both organs did not reveal any notable changes. These findings suggest the occurrence of C. burnetii infection in cattle within Bandung Regency, without specific histopathological changes indicative of Q fever lesions. In conclusion, presence of asymptomatic C. burnetii infections should be regarded as a significant threat to human health.
COMBINATION OF BASIL, TURMERIC AND BEAN SPROUTS TO HEMATOLOGY AND BIOCHEMISTRY OF FEMALE RAT BLOOD BEFORE PREGNANCY Andriyanto, Andriyanto; Mustika, Aulia Andi; Manalu, Wasmen; Subangkit, Mawar; Aurelia, Sharon; Widi, Leliana Nugrahaning; Putra, Hamdika Yendri; Tarigan, Elpita; Irarang, Yusa
Jurnal Kedokteran Hewan Vol 17, No 4 (2023): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i4.33189

Abstract

The purpose of the study was to examine the safety of combination of basil, turmeric and bean sprouts extract solution on the hematological parameters and blood biochemistry profile of rats. The combined ingredients used in the study were obtained from different places. A total of 15 rats were divided into 3 groups based on the treatment dose. Each group consisted of 5 rats. The rats in control group (K) did not given extract combination, while the rats in group KKT 1 % and KKT 5% received combination of basil, turmeric and bean sprouts extract at dose of 1% and 5%, respectively. Effectiveness and safety tests were carried out by evaluating the hematological and blood biochemical profiles of female rats. The data were analyzed using one-way analysis of variance (ANOVA). The results showed that the rats given combination of basil, turmeric and bean sprouts extract at a dose of 1% and, dose of 5% did not had significant different compared to control (P0.05), but tend to have positive effects in increasing several blood components that play an important role in maintaining immunity during pregnancy. It can be concluded that the combination of basil, turmeric and bean sprouts at dose of 1% and 5% do not adversely affect hematology profile and blood biochemistry of rats. This indicates that the extract combination does not cause any toxicity effects on the rats.

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