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Anneke Pesik
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Rumphius Pattimura Biological Journal
Published by Universitas Pattimura
ISSN : -     EISSN : 2684804X     DOI : https://doi.org/10.30598/rumphiusv5i1y2023
Core Subject : Science, Education, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology
Rumphius Pattimura Biological Journal membuka kesempatan untuk mempublikasikan tulisan ilmiah dalam bentuk artikel penelitian terbaru, short communication, maupun review terhadap artikel dari berbagai bidang biologi, dan cabang ilmunya seperti biodiversitas, mikrobiologi, ekologi, zoologi, botani, genetika, molekuler, bioteknologi, biosistematika, parasitologi, perilaku, pertanian, dan kehutanan. RPBJ diterbitkan oleh Jurusan Biologi û Fakultas MIPA, Universitas Pattimura. Dalam 1 tahun RPBJ memiliki 1 Volume dan 2 Issue. Dewan editor kami berasal dari berbagai rumpun ilmu biologi yang aktif dalam publikasi ilmiah skala Nasional dan Internasional, sehingga mampu melakukan proses review dengan adil dan profesional. Artikel yang diterima akan diterbitkan sesuai dengan waktu yang ditentukan yaitu di bulan Maret dan bulan September, dan akan tersedia gratis bagi pembaca
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 88 Documents
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ACTIVITY OF CLOVE LEAVES (Syzygium aromaticum L.) LARVACIDES ON MORTALITY OF Anopheles sp. Sapulette, Fenska Violenta; Unitly, Adrien Jems Akiles; Moniharapon, Debby
RUMPHIUS Vol 1 No 2 (2019): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv1i2p042-046

Abstract

This study used a completely randomized design with a sample of 525 Anopheles sp. larvae. which were divided into 7 groups and 3 replicates, which contained 25 Anopheles spper larvae. Except for the control, each group was given clove leaf infusion starting from a concentration of 0.50%, 1%, 2%, 4% and 6%, where the treatment was carried out for 24 hours. The results showed that there was a larvicidal effect of brewing clove leaves (Syzygium aromaticum L.) on the mortality of Anopheles sp mosquito larvae, where the effective concentration was 6% and had an LC50 value in killing Anopheles sp larvae of 0.89%. The overall phytochemical content of clove leaves which has a fast response to the mortality of Anopheles sp. are saponins, flavonoids and tannins which are capable of acting as larvicides through the mechanism of damaging cell membranes or interfering with larval metabolic processes. The content of saponins, flavonoids and tannins can increase the mortality of Aedes aegypti L. larvae through the mechanism of damaging cell membranes or interfering with larval metabolic processes as stomach poisoning.
ANTIHYPERURICEMIC EFFECT OF CLOVE LEAF (Syzygium aromaticum L.) ON DECREASING URIC ACID LEVELS IN BLOOD RAT (Rattus norvegicus) Laratmase, Nia; Nindatu, Maria
RUMPHIUS Vol 1 No 2 (2019): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv1i2p066-068

Abstract

This research is laboratory experimental in nature and was analyzed using Analysis of Variance and continued with Duncan's test, with 95% confidence (α=0.05) using SAS software. The results showed that giving clove leaf steeping could reduce uric acid levels in the blood of Rattus norvegicus rats. Clove leaf infusion (Syzygium aromaticum L.) contains flavonoids which have xanthine oxidase inhibitory activity, including luteolin, apigenin, kaemferol, and quercetin. In addition, Vitamin C can reduce oxidative stress and inflammation which affect the decrease in uric acid synthesis. Other compounds, namely tannins, alkaloids, and saponins have the same role as flavonoids, namely reducing the activity of the enzyme xanthine oxidase in serum and increasing the concentration of uric acid in the urine, as well as binding free radicals during the conversion of purines into uric acid. Infusion of clove leaves doses of 0.09g, 0.18g and 0.36g can reduce uric acid levels in the blood of rats (Rattus norvegicus) with an effective dose of 0.36g.
DYNAMICS COMMUNITIES OF BACTERIA AND YEAST IN PROCESSING ROBUSTA COFFEE WITH WET PROCESSING: SCREENING OF DOMINANT ISOLATES Nusaly, Windy Natalia; Pentury, Kresyan
RUMPHIUS Vol 2 No 2 (2020): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv2i2p043-049

Abstract

The type of coffee processing has a direct impact on the microbial community it contains and the quality of the coffee it produces. Coffee processing with a wet process (wet) is known to produce coffee with a good aroma and taste profile. This study aims to determine the dynamics of the bacterial and yeast community during the postharvest process and to determine the dominant microbes involved in the processing of Robusta coffee using a wet process. The method used was the isolation of bacteria and yeast using selective medium with the addition of antibiotics, then morphological identification and calculation of the index of diversity (H), dominance (D) and evenness (E) were carried out based on the bacterial and yeast isolates obtained, as well as screening of dominant isolates based on results of dominance analysis and enzymatic activity. The isolation results obtained by the yeast community (51.7%) with a higher number than the bacterial community (48.3%). The microbial community in the wet processing of Robusta coffee had a species diversity index (H') = 2.48, evenness (E) = 0.74 and dominance (D) = 0.17. The results of isolates screening through enzymatic tests showed bacterial isolates B10 and yeast Y2 were selected as dominant isolates and could be used as starter cultures in a controlled fermentation process.
ADMINISTRATED OF SNAKE WOOD (Strychnos lucida) TO DECREASED ABNORMALITY SPERMATOZOA OF MICE (Mus musculus) DIABETES MELLITUS MODELING Kunu, Marissa Beatrix; Baszary, Chomsa Dintasari Umi; Killay, Amos
RUMPHIUS Vol 2 No 2 (2020): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv2i2p037-042

Abstract

Diabetes mellitus, known as diabetes or blood sugar disease, is a group of chronic diseases characterized by increased blood sugar level (hyperglycemia). Prolonged hyperglycemia conditions will cause an increase in ROS (Reactive Oxygen Spesies) production by the mitochondria, which then cause damage to the mitochondrial membrane resulting in loss of function of the mitochondrial membrane potential and can induce sperm cells apoptosis. This study aims to determine effect of kayu ular (Strychnos lucida) steeping on decreasing spermatozoa abnormalities of mice (Mus musculus) diabetes mellitus model. The result showed that administration of kayu ular steeping at concentration of 2,6gr/50ml was signicantly reduced (P<0.05) spermatozoa abnormality. This dose is the most effective concentration to decreased the spermatozoa abnormality of mice. This though to be due to the active compounds found in steeping kayu ular (Strychnos lucida) which can counteract free radicals due to diabetes mellitus that decreasing spermatozoa abnormalities.
THE EFFECT OF LEMONGRASS EXTRACT (Cymbopogon citratus L.) ON PEST MORTALITY OF Plutella xylostella L. IN MUSTARD PLANTS (Brassica juncea L.) Moniharapon, Debby; Nindatu, Maria; Bastian, Alien
RUMPHIUS Vol 3 No 2 (2021): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv3i2p034-038

Abstract

Cabbage leaf caterpillars (Plutella xylostella L., Lepidoptera: Plutellidae) are the main pests that are very damaging to Brassicaceae plants, especially cabbage, mustard greens, and caisin in Indonesia. The chemical content of lemongrass is citral, citronella, geraniol, mirsene, nerol, farnesol methil heptenol and dipentene. The purpose of this study was to determine the effective concentration of Plutella xylostella pest mortality and the right LC50 value of Plutella xylostella pest mortality. This research is experimental using a completely randomized design. The subjects were 50 plutella xylostella L. Instar III pests. The concentrations used were 10 g, 20 g, 30 g, 40 g with negative control 0 g (aquades) with 5 repetitions for each treatment group. Observations were made 24 hours after spraying and the results obtained were an increase in pest death with increasing concentration. Based on the results of analysis of variance seen in the calculated F value (41,962)> F table (2,866). This proves that the administration of lemongrass stem extract significantly influences the mortality of Plutella xylostella in mustard plants (Brassisca juncea). The results of LC50 probit analysis were obtained at the concentration of lemongrass extract at 21,277% with a lower limit of 17,782 and an upper limit of 24,824, meaning that at a concentration of 21,277% lemongrass extract was able to kill 50% of the Plutella xylostella pest used after 24 hours of administration at a 95% confidence level.
ANTIBACTERIAL ACTIVITY AND INHIBITION OF BIOFILM OF Hibiscus tiliaceus STEM BARK METHANOL EXTRACT TO Streptococcus mutans GROWTH Lukaraja, Wati; Lessy, Widya; Seumahu, Cecilia Anna; Pesik, Anneke
RUMPHIUS Vol 2 No 2 (2020): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv2i2p066-071

Abstract

Dental caries is an infectious disease that occurs in the oral cavity preceded by the formation of dental plaque or biofilm. This study aims to examine the inhibition and biofilm inhibition of hibiscus tree bark extract (Hibiscus tiliaceus). The test methods used were Minimum Inhibitory Concentration (MIC) and Minimum Killing Concentration (MBC) while the analysis of biofilm inhibition used UV-VIS spectrophotometry at a wavelength of 580 nm with McFarlandII standard (6x108 CFU/ml). The results showed that the methanol extract of hibiscus bark at a small concentration of 0.5% could inhibit and kill Streptococcus mutans as indicated by the number of colonies that grew less with a value of 3.34 x 107 CFU/ml in colonies that grew at an extract concentration of 2. % and 1%. Biofilm inhibition is shown by the decreasing absorbance value due to the increased extract concentration so that less biofilm formation occurs.
IN VITRO ANTAGONISM OF THE ISOLATE MUSHROOM Trichoderma harzianum TO Fusarium oxyzporum f. sp. Cubense CAUSES WILT DISEASE IN BANANA KEPOK (Musa paradisiaca normalis) Pattikawa, Apriyanti Santi; Pagaya, Joseph; Patty, Jogeneis
RUMPHIUS Vol 2 No 2 (2020): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv2i2p050-055

Abstract

Trichorderma harzianum is a soil native fungus that is beneficial because it has high antagonistic properties against plant pathogenic fungi. Control mechanisms can be: antibiosis, parasitism, competition and hyphae interference. T. harzianum as a biological control agent for the pathogenic fungus Fusarium oxyforum f. sp. Cubense is an alternative to control this pathogenic fungus, without causing a negative impact on the environment. The aim of the study was to determine whether Trichodernma harzianum isolates Piru, Kairatu and Uraur could suppress the growth of the fungus Fusarium oxyforum f. sp. cubense and Trichorderma harzianum which isolates (Piru isolate, Kairatu isolate and Uraur isolate) had faster inhibition against Fusarium oxyforum f. sp. cubense causes wilt disease on banana kepok (Musa paradisiaca normalis) in vitro with antagonism test. The research was conducted in 2 places, namely in the HPT BPTPH laboratory in Maluku province and continued in the microbiology laboratory at FMIPA Unpatti. The research method used was laboratory experimental in nature by using a completely randomized design with factorial pattern with three treatments and three replications. The results showed that the cause of wilt disease in banana plants was Fusarium oxyforum f. sp. Cubense. Antagonism test in vitro showed that T. harzianum Piru isolate showed the highest inhibition zone (70.87%), both Kairatu isolates (45.27%), and the lowest by Uraur isolate (32.61%) so that it can be said that T. harzianum has the potential to inhibit the growth of Fusarium fungus. oxyforum f. sp. Cubense.
DIFFERENCES IN METAMORPHOSIS OF HONEY BEES Apis mellifera IN ROMANG ISLAND Mayaut, Gratia; Nindatu, Maria; de Kock, Rahel Hendrijete
RUMPHIUS Vol 2 No 2 (2020): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv2i2p056-059

Abstract

Honey bees (Apis mellifera) on Romang Island are scattered at several different points with abundant populations. The research location was carried out at two points, namely the first location is near residential areas, the height above sea level is approximately 10 meters, tends to be more crowded while the second location is far from settlements, the height above sea level is approximately 350 meters. The variables measured were the different metamorphosis phases of egg, larva, pupa, adult; temperature, food sources such as flowering plants, forest plants or fruit trees around the site. The difference in the time of metamorphosis of honey bees in the first location, seven days for laying eggs, five days for larvae, nine days for pupae, and seven days for adults while in the second location is six days for eggs, five days for larvae, ten days for pupae, and three days for adults. So the metamorphosis of honey bees in a quiet location and away from settlements shows a shorter duration compared to locations near settlements.
TRADITIONAL MEDICINE PLANTS OF WAAI VILLAGE COMMUNITIES, MALUKU PROVINCE Daro, Prichilia; Hiariej, Adriana; Nindatu, Maria
RUMPHIUS Vol 2 No 2 (2020): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv2i2p060-065

Abstract

This study aims to determine the types of plants used by the village community as traditional medicine and to find out how to use these types of medicinal plants. The research was conducted in Waai Village, Central Maluku District, Salahutu District. This research was conducted using survey methods and interviews with the community using a list of questions. The sampling technique was purposive sampling, the sample was determined as much as 15% of the total village population in Waai which consisted of 1500 heads of families and as many as 225 respondents were designated as heads of families. The results showed that there were 71 species of medicinal plants from 45 families. The most used plant parts are leaves, whole plants, fruit, roots, stems, rhizomes and tubers. Medicinal plants are generally used by the village community to cure ailments, aches and pains and to increase milk production. The utilization process is simple, by boiling, smoking, squeezing, rubbing and grating.
POPULATION STUDY OF GANDARIA (Bouea macropylla Grifith) IN RUMAHTIGA VILLAGE, AMBON Telussa, Ronald; Hiariej, Adriana; Lapu, Petrus
RUMPHIUS Vol 2 No 1 (2020): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv2i1p005-010

Abstract

This research was conducted to determine the gandaria population and the factors that influence it. The method used was the 10 m X 10 m plot method, with a total of 240 plot plots at 7 different stations based on the presence of gandaria plants. There were 976 trees with a density value of 0.09 ind/m2 or ± 1 individual/11 m2, with a cluster distribution pattern. Factors that affect the gandaria population are the burning of the gandaria population area, land conversion by humans, changes in plant types and factors of increased population growth.

Page 2 of 9 | Total Record : 88

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