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Ahmad Shafwan S. Pulungan
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Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Medan Jl. Willem Iskandar Psr V Medan Estate, Sumatera Utara
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JBIO: jurnal biosains (the journal of biosciences)
Published by Universitas Negeri Medan
ISSN : 24431230     EISSN : 24606804     DOI : https://doi.org/10.24114/jbio.v6i1
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Jurnal Biosains (JBIO) features works of exceptional significance, originality, and relevance in all areas of biological science, from molecules to ecosystems, (ie genetic, microbiology, ecology, biosystematic, biostatistic) including works at the interface of other disciplines, such as chemistry, medicine,physic and mathematics. We also welcome data-driven meta-research articles that evaluate and aim to improve the standards of research in the life sciences and beyond. Our audience is the international scientific community as well as educators, policy makers, patient advocacy groups, and interested members of the public around the world.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 269 Documents
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ISOLATION AND SCREENING OF AMYLASE PRODUCING BACTERIA FROM PALM OIL LIQUID WASTE Harmileni Harmileni; Rika Silvany; Tengku Rachmi Hidayani; Edy Fachrial
JBIO: jurnal biosains (the journal of biosciences) Vol 8, No 3 (2022): JBIO: jurnal biosains (the journal of biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v8i3.39886

Abstract

Amylase is the most studied enzyme in biotechnology and also accounts for 25% of the total enzyme market worldwide. In industry, amylase has potential applications in the bakery, textile, detergent, syrup, pharmaceutical, and medical industries. One of the potential sources of amylase is microbes, because it is easy to isolate, culture, and also genetically engineered. Liquid waste is one of the most potential and abundant sources of microbes. In this study, the authors used palm oil waste as a source of amylase-producing microbes. The purpose of this study was to isolate and screen bacteria that showed amylase activity. Bacteria were isolated from palm oil liquid waste samples using the serial dilution method and the number of CFUs was calculated. The isolates were then characterized morphologically including Gram staining, elevation, color, edge, cell shape and also catalase reaction. Characterization results obtained 7 gram positive isolates and 8 gram negative isolates, the shape of cocci (12 isolates) and bacilli (3 isolates) and convex elevation and white isolates. Amylase activity was detected using nutrient agar media supplemented with 1% starch and the amylolytic index was measured. Amylolytic activity was indicated by the presence of a clear zone around the isolate. Of the 15 isolates, only 1 isolate showed amylase activity, namely LCF 4 with an amylolytic index of 0.57. Further research is recommended to identify and determine enzyme activity.Keywords : Activity; amylase; bacteria; palm oil liquid waste
ETNOBOTANY OF PLANTS IN THE MANDAILING TRADITIONAL WEDDING SERVICE IN THE VILLAGE AROUND THE BATANG GADIS NATIONAL PARK AREA, MANDAILING NATAL REGENCY Levia Febrialisti; Aswarina Nasution
JBIO: jurnal biosains (the journal of biosciences) Vol 8, No 2 (2022): JBIO: jurnal biosains (the journal of biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v8i2.36497

Abstract

This study aims to document belief systems, local knowledge systems, plant utilization practices, and find out the cultural importance or ICS of plant species used in traditional Mandailing wedding ceremonies in villages around the TNBG area. The research method is qualitative and quantitative. Data was collected through semi-structured interviews, participatory observation, documentation, focus group discussions (FGD), collection of plant samples and making herbarium. The plants used in the traditional Mandailing wedding ceremony are 43 species which are included in 20 plant families. Plants with high ICS value were found in burangir (Piper betle L.), sontang (Uncaria rhynchophylla Miq.), pining (Areca catechu L.), and timbako (Nicotiana tabacum L.) with an ICS value of 96.
ETNOBOTANY OF TRADITIONAL MEDICINE PLANTS OF MANDAILING TRIBE IN THE SIMPANG TALAP RIVER FLOW REGION, RANTO BAEK DISTRICT, MANDAILING REGENCY Ofy Asyma Matondang; Dina Handayani
JBIO: jurnal biosains (the journal of biosciences) Vol 8, No 2 (2022): JBIO: jurnal biosains (the journal of biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v8i2.36388

Abstract

Documentation of the use of medicinal plants is a race against time. This traditional medical science is not documented, only conveyed orally, it is attractive to the elderly and less attractive to teenagers, causing this knowledge to be left behind and lost in one generation. This study aims to publish and document belief systems, utilization practices, local knowledge and cultural importance values of traditional medicinal plant species of the Mandailing Tribe. Location The research was conducted in Manisak Village, Ranto Baek District, Mandailing Natal Regency, which was held from December 2021 to March 202. Data was collected through semi-structured interviews, participatory observation, documentation, Collection of plant samples and the manufacture of herbarium. The data were analyzed descriptively qualitatively and quantitatively. The results showed that the Mandailing people used 44 species of medicinal plants which were included in 30 families. The highest family was Zingiberaceae 4% while the lowest family was Rutaceae 1%. The highest habitus of herb and the least habitus was liana. The practice of using plants by the Mandailing Tribe begins with meeting the datu, providing materials, materials in the form of plants obtained from the advice of the datu or parents who understand the pulungan of medicinal plants, the method of processing is mostly boiled and the method of use is mostly by drinking. Eme (Oriza sativa) with an ICS value of 71, its use is as a medicinal ingredient, a staple food ingredient that has no substitute, and traditional ritual events (mythology). Keywords: Ethnobotany, Traditional Medicine, Mandailing Tribe Belief System, utilization practices, local knowledge, Values of cultural importanc.
MORFOMETRIC ANALYSIS OF MANGROVE CRAB (Scylla serrata) IN TANJUNG REJO VILLAGE, PERCUT SEI TUAN DISTRICT, DELI SERDANG REGENCY, NORTH SUMATERA Rifqah Amalia; Puji Prastowo
JBIO: jurnal biosains (the journal of biosciences) Vol 8, No 2 (2022): JBIO: jurnal biosains (the journal of biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v8i2.36543

Abstract

This study aims to determine the character that contributes the most to the body weight of mud crab (Scylla serrata) and the morphometric differences between male and female mangrove crabs (Scylla serrata). The research was carried out in Tanjung Rejo Village, Percut Sei Tuan District, Deli Serdang Regency, North Sumatra from March to April 2022. This research is quantitative descriptive with explorative methods, namely direct sample collection or Hand sorting. The morphometric parameters observed were carapace width, carapace length, carapace height, optical groove widths, chela length, chela height and deep length. The analysis used is multiple linear regression with stepwise method and t test. The results of the study showed that the morphometric characters that contributed the most to body weight in male mud crabs were the length of the right chela (93%) and carapace length (94.1%), while in female mud crabs, carapace height (87%) and carapace width (91%) and there is a very significant difference between male and female mud crab morphometrics on the characters of optical groove widths, right chela length, right chela height, right chela deep length, left chela length, and chela height. on the left and deep length on the left. Meanwhile, significant characters were found in carapace width and carapace height. For carapace length characters showed no significant difference.
POTENTIAL OF WULUH LEAVE (Averrhoal bilimbi L.) AS ANTIFUCIAL Candida albicans Weni Astari; Elisa Putri
JBIO: jurnal biosains (the journal of biosciences) Vol 8, No 3 (2022): JBIO: jurnal biosains (the journal of biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v8i3.38549

Abstract

Wuluh starfruit in Aceh commonly called "Boh Limeung" is a plant that is very often encountered, because star fruit is one of the spices in cooking. The Acehnese use star fruit as "SUNTI ACID", which is star fruit that is dried and then salted and used as a spice in cooking. The chemical compounds in star fruit leaves are flavonoids, saponins, sulfur, formic acid, steroid peroxides and tannins which are able to inhibit microbial activity and damage cell membranes so that, they can inhibit bacterial growth. The purpose of this study was to determine the potential of starfruit leaves (Averrhoal bilimbi L.) as antifungal candida albicans. The method used in this research is experimental laboratory. The treatments given in this study were 30%, 40%, 50%, 60% methanol extract concentrations. Then for the positive control (+), ketoconazole was used and for the negative control (-) aquadest was used. Based on the results obtained from the methanol extract of starfruit leaves (Averrhoal bilimbi L.) at concentrations of 30%, 40%, 50%, 60%, the average inhibition zone values were 17.5 mm, 17.35 mm, 17.95. mm, 18.77 mm. In Table 4.2, it can be seen that the largest inhibition zone is in the positive control (+) which is 23.5 mm while the negative control (-) is 0 mm.
PHYTOCHEMICAL SCREENING OF LICHENS EXTRACT Usnea sp. ON PINES IN THE BARRIAN HILL FOREST, NORTH SUMATRA Ashar Hasairin; Ahmad Shafwan S Pulungan; Adi Hartono
JBIO: jurnal biosains (the journal of biosciences) Vol 8, No 3 (2022): JBIO: jurnal biosains (the journal of biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v8i3.42602

Abstract

Lichen is an organism that has a high level of biodiversity and contains secondary metabolites. This study aims to determine the secondary metabolites of lichen extract Usnea sp. on Pine. Qualitative descriptive research method. Preparation of Usnea sp. extract. carried out by maceration method using 96% ethanol (1:10) for 5 days. The results of the study based on the phytochemical screening of the ethanol extract of lichen Usnea sp. contains alkaloids, flavonoids, saponins and tannins and triterpenoids. The results of the phytochemical screening showed a positive (+) result indicated by a change in color or the formation of a precipitate or the formation of foam after adding reagents to the test extract. Meanwhile, a negative result (-) is indicated by the absence of a change in color or the formation of precipitate or the formation of foam after adding reagents to the test extract.
EFFECT OF ETHANOLIC EXTRACT OF MELINJO PEEL (Gnetum gnemon) ON SERUM MALONDIALDEHYDE (MDA) LEVELS IN HYPERURISEMIA WISTAR STRAIN RATS Indra Priawan; Yetty Machrina; Ririe Fachrina Malisie
JBIO: jurnal biosains (the journal of biosciences) Vol 8, No 3 (2022): JBIO: jurnal biosains (the journal of biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v8i3.43187

Abstract

Hyperuricemia is a state of uric acid in the body that is higher than the body's tolerance limit. The formation of uric acid will be accompanied by the formation of Reactive Oxygen Species (ROS) by the changes of Xanthine Dehydrogenase to Xanthine Oxide with the help of NAD+ and Oxygen, which can trigger oxidative stress, causing cell damage and apoptotic cell, one of which is through lipid peroxidation followed by the release of Malondialdehyde (MDA) into the extracellular space. This study aims to analyze the effect of giving melinjo peel ethanol extract (Gnetum gnemon) on serum MDA levels in hyperuricemic Wistar strain rats. Rats were grouped into 4 treatment groups, namely K0 (without treatment), Kneg (hyperuricemia and CMC), Kpos (hyperuricemia and allopurinol), and P (hyperuricemia and ethanol extract of melinjo peel). Manufacture a hyperuricemic rat model by giving a high-fructose diet and then measuring uric acid levels to ensure that the rat is already in hyperuricemia. The high-fructose diet was continued until the rats were executed. Serum MDA was measured using the Thiobarbituric acid (TBA) method. The results showed differences in MDA levels between groups of hyperuricemic rats. Based on the examination of lower MDA levels after administration of melinjo peel ethanol extract compared to the CMC group and the allopurinol group, statistically significant differences were obtained (p = 0.001). Based on the analysis results, it was concluded that the ethanolic extract of melinjo peel (Gnetum gnemon) could reduce serum MDA levels in hyperuricemic Wistar rats.
TEST OF FORMAL AND PHYSICAL PROPERTIES FOR PREPARATION OF TOFUMASA KOLANG-KALING SEED POWDER COMBINE (Arenga pinnata L.) CREAM SHELL Sofia Rahmi Tambusai; Anggun Syahfitri
JBIO: jurnal biosains (the journal of biosciences) Vol 9, No 1 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i1.39154

Abstract

Tofu waste is waste from the tofu processing industry that has hardly been used except as animal feed or simply thrown away. Kolang Kaling is widely used as a processed food in the community. The aim of this study was to prepare creamy peeling formulas from tofusae and kolang-kaling seeds. This study started with the preparation of tofu powder and tofu clay, then a creamy exfoliating preparation was made from the second combination in the ratio (1:1; 1:3 and 3:1). Physical testing of the cream peeling formulations was then performed in the form of organoleptic, spreadability, stickiness, cream type, protection test, pH test and preference test. The results obtained in the form of Formula I with the ratio (1:1) are the best compared to the other two formulas.
METABOLITE ACTIVITY OF LACTIC ACID BACTERIA FROM SUMBAWA HORSE MILK, WEST NUSA TENGGARA, INDONESIA Kusdianawati Kusdianawati; Apon Zaenal Mustopa; Fatimah Fatimah; Nurlaili Ekawati
JBIO: jurnal biosains (the journal of biosciences) Vol 9, No 1 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i1.37701

Abstract

Sumbawa horse milk is one of the local products in Sumbawa (West Nusa Tenggara). There is no research so far which discusses the potencies of LAB metabolite compounds as antimicrobials and probiotics for treating Diabetes Mellitus type 2. This study aims to obtain the antimicrobial and inhibitory activity of the LAB-extracted metabolite compounds from Sumbawa horse milk collected from Penyaring village and Lennanguar village, Sumbawa, West Nusa Tenggara, Indonesia. LAB metabolites are extracted by using 96% ethanol. It has been found that metabolites extracted from media culture of LAB SKP K.9 (E. thailandicus) have the highest antimicrobial activity against S. aureus (ATCC 6538) (2.5 cm), S. typhimurium (2.5 cm), L. monocytogenes (BTCC B693) (2.53 cm) and EPEC K.1.1 (2.7 cm) when compared with other LAB isolates. Moreover, the inhibition of α-glucosidase enzyme obtained from LAB SKP K.5 isolate (L. garvieae) is 6.25%, 39.28% from LAB SKP K.9 (E. thailandicus), 53.57% from LAB SKP K.3 (W. confusa), 67.85% from LAB SKL K.4 (L. fermentum), and 78.57% from LAB SK 1.5 (E. faecium). The extract of LAB SK 1.5 metabolite compound (E. faecium) has the highest inhibitory activity against the α-glucosidase enzyme (78.57%) compared to other LAB isolates.
THE EFFECT OF ETHANOL EXTRACT OF PIRDOT (Saurauria vulcani) ON HEMATOLOGICAL PROFILE AND KEAP1-NRF2 INHIBITION OF WHITE RATS INDUCED RHODAMINE B Adriana Yulinda Dumaria Lumban Gaol; Erlintan Sinaga; Rosinta Febryanti Simamora; Feimmy Ruth Pratiwi Sipahutar; Hudson Sidabutar
JBIO: jurnal biosains (the journal of biosciences) Vol 9, No 1 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i1.44136

Abstract

The study was designed to evaluate the hematological effect of ethanol extract of Pirdot (Saurauia vulcani) (EES) in white rats induce rhodamine B and predict interaction of bioactive compound Pirdot to bind active site Keap1-Nrf2. Twenty- four Male Wistar rats (100-200 g) divided into four groups. Group P1 served as group control is administered with CMC 0.5%; group P2 is treated with Rhodamine B 750 mg/kg BW, Group KP1 administered EES 500 mg/kg BW; and Group KP2 is treated with EES 500 mg/kg BW+ Rhodamine B 750 mg/kg BW. Hematological parameters were assessed. The results revealed that red blood cell (RBC), white blood cell (WBC), thrombocyte count, and hemoglobin concentration (Hb) in rats induced Rhodamine B significantly lower than the control group. However, EES could improve the value of hematological profile. Our finding demonstrated that EES normalizes the value of hematological parameters in rats induce Rhodamine B. Moreover, beta-amyrin, pomolic acid and maslinic acid from Pirdot had good binding affinity to Keap1-Nrf2

Page 20 of 27 | Total Record : 269

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