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Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions
JBBI is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original research papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees, and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, bioinformatics, as well as life sciences in general.
Arjuna Subject : Umum - Umum
Articles 28 Documents
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Search results for , issue "Vol. 12 No. 2 (2025)" : 28 Documents clear
RECENT UPDATE OF ZERUMBONE AS ANTI-COLON CANCER AGENT: A REVIEW Sarmoko, Sarmoko; Fauziyya, Riri; Zahra, Miralda; Suprahman, Nisa Yulianti; Auli, Winni Nur; Ashari, Arif
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.9338

Abstract

Colorectal cancer (CRC) remains a leading cause of cancer-related deaths worldwide, with limited effective therapies due to drug resistance and adverse effects. Zerumbone, a sesquiterpene isolated from Zingiber zerumbet Smith, has emerged as a promising natural anticancer agent. This review examines zerumbone's anti-colorectal cancer properties, including induction of apoptosis and cell cycle arrest, inhibition of invasion and metastasis, anti-angiogenic activity, and anti-inflammatory effects. Additionally, zerumbone demonstrates antioxidant properties, modulates gut microbiota composition, and targets multiple signaling pathways involved in CRC pathogenesis. Structure-activity relationship studies reveal the critical role of the α,β-unsaturated carbonyl group in its bioactivity. Despite promising preclinical evidence, clinical validation remains necessary to establish zerumbone's therapeutic potential for colorectal cancer management.
COMPARATIVE ASSESSMENT OF BULL SPERM MOTILITY AND CONCENTRATION: CONVENTIONAL METHODS VERSUS PORTABLE ANDROSCOPE CASA SYSTEM Arifiantini, Raden Iis; Gusman, Khalis Talitha; Agil, Muhammad; Karja, Ni Wayan Kurniani
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10400

Abstract

Operational implementation, such as laboratory staff and equipment, differ for each frozen semen producer, resulting in variability in the quality assessment of the frozen semen produced. Conventional assessments subject to subjectivity, human error, and high variability. Computer-assisted semen analysis (CASA) is considered more objective. This study aimed was to conduct a comparative analysis of motility and concentration assessment of frozen semen sperm using conventional and the portable CASA AndroScope. Twenty-one laboratory assistants from 21 national and regional frozen semen producers participated in this study. Sperm motility and concentration were assessed conventionally (using a Neubauer chamber) and AndroScope was used with five replicates. The results of the comparison of sperm motility scores revealed significant differences (p<0.05) between conventional examination and using AndroScope. The results of the comparison of the sperm concentration calculations revealed no significant difference (p>0.05) between the calculations performed using the Neubauer chamber and AndroScope. Assessment with the AndroScope is considered reliable and can replace conventional assessment in assessing motility and sperm concentration, thereby improving and standardizing the assessment performed by frozen semen producers.
CHARACTERISTICS AND KINEMATICS OF RAM FROZEN-THAWED SPERM MOVEMENT WITH DIFFERENT LECITHIN TYPES Damanik, Asman Ramadhan; Karja, Ni Wayan Kurniani; Setiadi , Mohamad Agus; Kaiin, Ekayanti Mulyawati
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10407

Abstract

This study evaluated the effectiveness of animal, plant, and synthetic lecithin-based extenders in preserving local Indonesian ram semen during cryopreservation. Post-thaw sperm quality was assessed using Computer-Assisted Sperm Analysis (CASA), focusing on motility, viability, membrane integrity, and kinematics. Data were analyzed using ANOVA and Duncan’s Multiple Range Test (DMRT) at a 95% significance level. Results showed that sperm motility, viability, and membrane integrity were significantly higher (P<0.05) in the animal lecithin-based extender (53.66±1.33%, 66.45±2.50%, and 67.68±2.33%, respectively) compared to plant (51.84±0.48%, 62.98±1.94%, and 64.27±1.51%) and synthetic (50.14±0.22%, 60.72±0.81%, 62.44±1.37%) extenders. While kinematic parameters were not significantly different among groups, synthetic lecithin showed slightly higher values in velocity-related measures, whereas animal lecithin resulted in the most consistent improvement in overall sperm quality. All lecithin-based extenders met the Indonesian National Standard (SNI) for semen quality, and sperm kinematics remained within optimal fertilization values after cryopreservation.
OPTIMIZATION AND EFFICIENCY ANALYSIS OF REAL-TIME PCR FOR LEPTOSPIRA SPP. DIAGNOSIS BASED ON THE LIPL32 GENE Sulaeman, Rizal Pratama; Rohayati; Merdekawati, Fusvita; Sundara Mulia, Yuliansyah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10646

Abstract

Leptospirosis is a widespread zoonotic infection that endangers the health of both humans and animals, particularly in tropical and subtropical regions. Therefore, timely, sensitive and specific laboratory confirmation is essential for early clinical management. The LipL32 gene is a highly conserved virulence factor in pathogenic Leptospira. Real-time PCR provides rapid detection with high sensitivity and specificity. This study optimized real-time PCR conditions by evaluating annealing temperatures (60°C, 61°C, 62°C), primer concentrations (500 nM, 700 nM, 900 nM), and probe concentrations (250 nM, 300 nM). PCR efficiency was analyzed using absolute quantification with serial DNA dilutions (100 to 10⁻⁴). The optimal conditions were 60°C annealing temperature, 500 nM primers, and 300 nM probes. Near-ideal efficiency (97%) was achieved at 60°C with 500 nM primers and 250 nM or 300 nM probes, while 103% efficiency was observed at 61°C with 500 nM primers and 250 nM probes. This optimization enhances Leptospira detection accuracy using real-time PCR.
VIOLACEIN: IT'S ANTICANCER PROPERTIES Santoso, Adit Widodo; Simamora, Adelina; Margretha, Margretha; Timotius, Kris Herawan
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11333

Abstract

Violacein is a bacterial secondary product with various bioactivities, including anticancer activities. This narrative review aimed to evaluate anticancer potentials based on its modes of action, either at cellular, subcellular, or molecular levels or in tumour microenvironment. At cellular level, violacein can inhibit cancer cell proliferation, arrest cell cycle, induce apoptosis, autophagy, and cell differentiation. At subcellular level, violacein can modulate processes in mitochondria. At molecular level, violacein can generate reactive oxygen species, attenuate inflammation, repair oncogenes, upregulate suppression genes, inhibit or activate several cancer vital enzymes, and control various signalling pathways. Violacein indirectly influences communication between cancer cells and their tumour microenvironment by inducing apoptosis and autophagy and inhibiting metalloproteinases and angiogenesis. Violacein inactivates several signalling pathways, including MAPK, Akt/NF-kB, JAK2/STAT3, and TGFβ, which are essential for cancer cell development. Violacein is a promising anticancer drug candidate with broad coverage of various cancer diseases and diverse modes of action.
ANALYSIS OF THE RATIONALITY OF ANTIPLATELET USE IN ISCHEMIC STROKE PATIENTS AT “X” HOSPITAL, SEMARANG CITY Wibowo, Sulistiyanto; Dian A, Yustisia; Hasanah A.M, Restu
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11402

Abstract

Ischemic stroke accounts for approximately 85% of all stroke cases and remains a major global health problem. Antiplatelet agents are key for secondary prevention, but irrational use may reduce effectiveness and increase bleeding risk. This retrospective descriptive study evaluated the rationality of antiplatelet use in ischemic stroke patients at “X” Hospital, Semarang City, from January to July 2024. Patients aged ≥26 years with complete medical records were included. Rationality was assessed based on PNPK Stroke Guidelines (2019), AHA/ASA Guidelines (2021), and AHFS Drug Information (2024) using three indicators: appropriate drug, dose, and patient. A total of 111 patients (113 cases) met the inclusion criteria. Most were male (58.56%) and aged >65 years (36.04%), with hypertension, diabetes, and hyperlipidemia as common comorbidities. Aspirin was the most used monotherapy (38.05%), and aspirin–clopidogrel the most frequent combination (37.17%). Drug selection accuracy was high (96.46%), while dose accuracy was low (44.25%). Antiplatelet use at “X” Hospital demonstrated rational drug selection but suboptimal dosing. Improved adherence to dosing guidelines and periodic prescription review are needed to enhance patient safety and treatment outcomes.
IMPROVEMENT OF SHEEP OOCYTE COMPETENCE IN VITRO THROUGH GLUTATHIONE ADDITION Yusmala, Mitha; Supriatna, Iman; Setiadi, Mohamad Agus
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11512

Abstract

Oocyte competence is a primary factor for the success of embryo production in vitro. This research aims to determine the effect of supplementation glutathione in sheep oocytes’ maturation and fertilization rates in vitro. In the first research, maturation oocytes were conducted in a medium supplemented with GSH at graded doses for 24 hours. In the second research, 1.0 mM GSH (the best dose from research I) was supplemented in the maturation medium, fertilization medium, and their combination for 14 hours. The higher oocyte maturation rates were significant (P < 0.05) in the GSH supplementation of 1.0 mM (87.00%). Furthermore, supplementation of GSH on the maturation medium resulted in a higher normal fertilization rate (56.43%) than in other groups. It is concluded that GSH supplementation on the maturation medium is more effective in increasing the competence of sheep oocytes to be mature and results in improved fertilization rates.
IDENTIFICATION OF SECONDARY METABOLITE COMPOUNDS USING GC-MS IN METHANOL EXTRACTS OF KEISSI TUBERS (Stephania venosa (Blume) Spreng) Salempa, Pince; Salnus, Subakir; Hasri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11632

Abstract

Traditional medicinal plants are an important source of bioactive compounds that have the potential to be developed as raw materials for health products. One species used by the community in Mambi District, West Sulawesi, for cancer treatment is Belajang Keissi' (Stephania venosa (Blume) Spreng). This study aims to identify the secondary metabolites contained in the methanol extract of Belajang Keissi' tubers. The extraction process was carried out using the maceration method with methanol as the solvent, followed by qualitative phytochemical testing and compound profile analysis using GC-MS. The results of the phytochemical test showed that the methanol extract of Belajang Keissi' tubers contained alkaloids and flavonoids. Further GC-MS analysis revealed the presence of several dominant compounds, namely 9,12-octadecadienoic acid (Z,Z)-, methyl ester; trans-13-octadecenoic acid, methyl ester; methyl stearate; 6H-dibenzo[a,g]quinolizine, 5,8,13,13a-tetrahydro-2,3,9,10-tetramethoxy-; and (±)-2,3,3′,4′-tetramethoxy-α-methyl-5-(prop-1-enyl)stilbene. These findings indicate that Belajang Keissi tubers have potential as a source of bioactive compounds that can support the development of traditional medicines based on natural ingredients.
ISOLATION OF ANTIBIOTIC PRODUCING MICROORGANISMS FROM CASSAVA ROOT TAPAI BY TRADITIONAL FERMENTED Muhiddin, Nurhayani H.; Hidayah, Nurul; Herawati, Netty; Ramlawati; Sukmadewi. M, Fitrah; Darmayani , Dwi; Marnia; Idris, Nur Afni Yunita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11634

Abstract

Antibiotic resistance among pathogenic microorganisms has become a growing global health concern, necessitating the search for new natural sources of antimicrobial compounds. Cassava tapai, a traditional Indonesian fermented food, contains molds, yeasts, and bacteria with potential antibiotic-producing abilities. This study aimed to isolate and characterize antibiotic-producing microorganisms from cassava tapai fermented with local Sulawesi ‘Ragi’. Isolation, colony morphology characterization, and antibiotic activity tests were conducted using the Kirby–Bauer method against Escherichia coli ATCC 35218 and Staphylococcus aureus ATCC 25923. Inhibition zones were measured with a digital caliper, and data were analyzed using one-way ANOVA followed by Tukey’s HSD test or Kruskal–Wallis followed by the Mann–Whitney U test (p < 0.05). Three isolates Bb1, Bb2, and Wk3 exhibited significant antibiotic activity, with Bb1 showing stronger inhibition against E. Coli ATCC 35218. These results highlight confirms the potential of cassava tapai as a promising source of antibiotic-producing microorganisms for future antimicrobial discovery.
THE ROLE OF AgNPs AND AB Mix IN THE GROWTH OF Aglaonema 'LADY VALENTINE' IN VITRO & EX VITRO Larrisa, Damasa Ines; Nugrahani, Pangesti; Wiyatiningsih, Sri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11917

Abstract

Aglaonema ‘Lady Valentine’ is a popular ornamental plant with significant economic value. Conventional propagation methods have limitations, making tissue culture and acclimatization viable alternatives. However, low shoot multiplication and slow acclimatization remain major challenges. This study investigated the effects of silver nanoparticles (AgNPs) and AB Mix nutrient concentrations on in vitro and ex vitro plantlet growth using a two-factorial completely randomized design with four levels and three replications. The results showed varied growth responses, with the best performance observed in the 2 ppm AgNPs and 3 ml/L AB Mix treatment, which increased plantlet height, volume, and visual health. In ex vitro conditions, both 2 ppm and 3 ppm AgNPs combined with 3 ml/L AB Mix supported better plantlet adaptation, as seen in increased height, number of leaves and shoots, and seedling viability. Overall, the combination of 2 ppm AgNPs and 3 ml/L AB Mix proved most effective for enhancing multiplication and acclimatization

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