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Pharmaciana: Jurnal Kefarmasian

PHARMACIANA
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Published by Universitas Ahmad Dahlan

ISSN : 20884559 EISSN : 24770256 DOI : 10.12928

Core Subject : Health,

Medicine & Pharmacology

Pharmaciana is a scientific journal published by the University of Ahmad Dahlan worked closely with Ikatan Apoteker Indonesia (IAI). Pharmaciana published three times a year, namely March, July and November. with ISSN 2088-4559 and e-ISSN 2477-0256. The article published in the Journal Pharmaciana selected by editors and reviewed by the reviewer. Articles published in Pharmaciana must not be published in other journals or have been previously published. Pharmaciana is indexed in google scholar, ACI (Asean Citation Index), Dimension (Crossreff), Garuda, Sinta, Sherpa Romeo, Index Copernicus International, DOAJ, and BASE. Pharmaciana is accredited by DIKTI (DGHE) of Indonesia No. 105/E/KPT/2022 April 07, 2022

Arjuna Subject : Kimia(semua kategori) - Kimia (Lain-Lain)

Articles 808 Documents

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Validation of TLC densitometry method for the quantitative determination of alkaloid in fermented endophytic fungi extract Phyllantus niruri Linn Rollando rollando; Sandra Dewi Tansil; Eva Monica; Yuyun Yuniati; Leny Yuliati
Pharmaciana Vol 9, No 1 (2019): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Endophytic fungi can produce compound that similar with the host, so it can beÂ used as mass production of compound. The optimal and valid assay method is needed to obtain the proper culture condition, which one using densitometric Thin Layer Chromatography (TLC). This research used 11 samples of endophytic fungi culture extract from Phyllantus niruri within various culture condition. Optimization of mobile phase was done using 3 kinds of mobile phase mixture, chloroform: ethyl acetate: methanol (8:8:4); chloroform: ethyl acetate: methanol: NH4OH (8:8:4:0.005); chloroform:ethyl acetate:NH4OH (8:8:0.005). Validation method measured using some parameters such as linearity, accuracy, and precision. The result of optimization and validation showed that TLC densitometry method can be used for measuring alkaloid level within the endophytic fungi extract of P. niruri using chloroform: ethyl acetate: NH4OH (8:8:0.005), with R value is 0.977, percentage of recovery is between 90â€“110, and RSD â‰¤7% on each concentration series. While the proper condition of endophytic fungi culture is using sucrose as carbon source and at pH 6 condition. The content of alkaoid reached 25.9 Â± 1.4 mg 100 g-1 fresh weight.Â

Antioxidant and alpha-amylase inhibitory study of Sansevieria trifasciata Prain. leaves extract Vivi Anggia; Lusi Putri Dwita; Istikomah Istikomah
Pharmaciana Vol 9, No 1 (2019): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Diabetes is one of the chronic diseases whose prevalence is increasing all over the world, including in Indonesia. Diabetes mellitus is characterized by high blood glucose levels that can be overcome by, for instance, inhibiting the alpha-amylase enzymes that play a role in carbohydrate hydrolysis. Several studies have proved that active antioxidant substances can also support the treatment of diabetic patients. Snake plant (Sansevieria trifasciata Prain.), or locally known as lidah mertua in Indonesia, is a traditional herbal used to treat diabetes. This study investigated the activity of the ethanol extract of S. trifasciata leaves in inhibiting Î±-amylase. The leaves were extracted by maceration with ethanol 70%, followed by phytochemical screening. The antioxidant activity was determined using the DPPH method, while the inhibition of Î±-amylase was measured using UV-Vis spectrophotometer at 524nm wavelength. The results showed that IC50 of the antioxidant activity was 1527.55 ppm, five times greater than the positive control, i.e., vitamin C. In the case of Î±-amylase enzyme, the IC50 of the ethanol extract of S. trifasciata leaves was 158.31 Î¼g/ml and 0.26 times more potential than acarbose. The study has proved that the crude extract of S. trifasciata leaves has the potential as one of the antidiabetics and antioxidants that can be studied further.Â

Potential of phenolic compounds in persimmon fruit extract (Diospyros Kaki L.) against atherosclerosis in rats Alhara Yuwanda; Endang Hanani; Fadhlina Chani Saputri
Pharmaciana Vol 9, No 1 (2019): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Persimmon fruits (Diospyros kaki L.) contain catechins and epigallocatechin gallate (EGCG) which have antioxidant properties. The study aimed to prove the antioxidant potential of phenolics in persimmon fruit (D. kaki L.) in reducing oxidation of LDL and as antiatherosclerotics. The research method included an anti-oxidant test using a UV-VIS spectrophotometer and measurement of polyphenol level using High-Performance Liquid Chromatography (HPLC). Also, blood serum levels were examined in vitro and in vivo based on the histopathology of the aorta of male Sprague-Dawley rats subjected to a cholesterol diet. There were five test groups: positive control (receiving Î±-tocopherol), negative control, dose I (administered with the extract at 450 mg/kg BW), dose II (extract 900 mg/kg BW), and dose III (extract 1800 mg/kg BW). The testing was carried out for eight weeks. Antioxidant test using DPPH method produced an IC50 of 44.07Â±15.06 mg/mL. The polyphenol contents identified in the persimmon extract were catechins 2.55% and EGCG 9.49%. The results showed that the persimmon extract exhibited antioxidant activity that prevented atherosclerosis through the mechanism of LDL oxidation. At a concentration of 1,800 mg/kg BW, the extract decreased the aortic wall thickness by 31.20% and showed activities that were not significantly different from the positive control (p value= 0.05).

Anti-inflammatory activity of essential oil of clove (Syzygium aromaticum) in O/W and W/O Creams Nining Sugihartini; Gina Lestari; Sapto Yuliani
Pharmaciana Vol 9, No 1 (2019): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Previous studies have shown that the formulations of clove bud essential oil (CBEO) with a concentration of 5% in O/W cream and 2.5% in W/O cream have anti-inflammatory properties. The formulations are developed by adding enhancers, namely oleic acid and propylene glycol. The optimum compositions of oleic acid and propylene glycol are 50%:50% in W/O cream and 30%:70% in O/W cream. This study aimed to determine the effects of different compositions of creams on their anti-inflammatory activities. The creams were evaluated for their anti-inflammatory properties using mice with croton oil-induced inflammatory. The mice were sacrificed, and the back skin was removed to make histopathologic preparations at the end of the three-day inflammatory induction to obtain data of epidermal thickness and the number of cells with COX-2 expression. The results showed that the addition of oleic acid and propylene glycol as enhancers increased the anti-inflammatory activity of both O/W and W/O creams. Since the O/W cream decreased the thickness of the epidermis and the number of cells with COX-2 expression more than the W/O cream, the formulation of CBEO in O/W cream is concluded as a better anti-inflammatory than that of W/O cream.Â Â Â

Construction of recombinant sox2-encoding plasmids that regulate pluripotency of breast cancer stem cells from indonesian patient Hanifah Rahmi; Tiodinar Theresia; Amarila Malik; Septelia Inawati W
Pharmaciana Vol 9, No 1 (2019): Pharmaciana
Publisher : Universitas Ahmad Dahlan

A therapy development with recombinant protein is a new and potential innovation to destroy cancer stem cells (CSCs). Various ways of killing CSCs include the provision of polyvalent anti-protein antibodies that code pluripotency. Therefore, it takes a mixture of Oct-4, c-Myc, Sox2, and Klf4 protein antigens that can stimulate the formation of polyvalent antibodies. This study aimed to construct Sox2 recombinant by identifying the target genes by reverse transcriptase PCR and then arranging their designs to be inserted into the cloning vector pET101/D-TOPOÂ®. The target gene was developed by finding the complete sequences of Sox2 nucleotides on the NCBI GenBank. The growth on LB-ampicillin agar plates was amplified by PCR to obtain colonies with pET101/D-TOPOÂ® vectors and inserts of the pluripotent gene of CSCs, then the PCR results were observed through electrophoresis. A total of fifteen colonies have DNA bands with a base pair of about 300 bp in length. The recombinant clones produced Sox2 genes with a base length of 330 bp.

Total flavonoid contents and in silico study of flavonoid compounds from Meniran (Phyllanthus niruri L.) towards alpha-amylase and alpha-glucosidase enzyme Sri luliana; Rise Desnita; Ronny Martien; Arief Nurrochmad
Pharmaciana Vol 9, No 1 (2019): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Meniran (Phyllanthus niruriÂ L.) is a plant that is reported to contain flavonoids and shows the activity as a type 2 anti-diabetic by a mechanism of inhibiting the absorption of glucose.Â Flavonoid compounds such as rutin, quercetin and hesperidin shows anti-diabetic activity.Â This research aims to determine the levels of total flavonoids andÂ dockingÂ studies of flavonoids from meniran against Î±-amylase and Î±-glucosidase.Â Analysis of content of total flavonoids utilizes colorimetric method using AlCl3 and routine standard, the docking study usesÂ Autodock VinaÂ (version 1.1.2) programÂ with the assistance ofÂ AutoDockToolsÂ (ADT) and visualization of docking results using Discovery Study.Â The results of the determination of total flavonoids level was 12.32 Â± 0.53 mgRE/g extract whereas in ethyl acetate fraction and water each were 19.33Â±0,68 and 20.07Â±1.23 mgRE/g respectively.Â Results of all flavonoids dicking study shows the potential to bind to both receptors, rutin has better binding energy towardsÂ Î±-amylase whereas against Î±-glucosidase is demonstrated by 3-OÂ -Î²-D-glucopyranosyl-(2-1)-O-Î²-D-xylopyranoside quercetin.

Solvent concentration effect on total flavonoid and total phenolic contents of Averrhoa bilimbi leaf extract Muhammad Ryan Radix Rahardhian; Bayu Tri Murti; Dyah Wigati; Ririn Suharsanti; Chintiana Nindya Putri
Pharmaciana Vol 9, No 1 (2019): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Averrhoa bilimbi is one of the Indonesian indigenous plants containing phenolic and flavonoids which exhibit several ethnopharmacological effects i.e. antidiabetic, anti-microbial, anti-inflammatory, cytotoxic, anti-oxidant, antifertility, and antibacterial activities. However, the optimum solvent for extracting these compounds using percolation have not been reported, yet. This research was aimed to evaluate the various concentrations of ethanol solvents towards total phenolic (TPC) and total flavonoid content (TFC). Extraction was carried out using percolation technique with ethanol solvent ratio of 50%, 70%, and 96%. The phytochemical screening, thin layer chromatography, total flavonoids, and total phenolic levels were obtained from the concentrated extract. The TPC and TFC were determined using visible spectrophotometry method. Phytochemical screening study indicated that ethanolic extract contains classes of flavonoids, phenolic, alkaloid, saponins, and steroids. The TFC of ethanolic extract with 50%, 70%, and 96% solvent concentrations were investigated at 62.74, 64.81 and 59.1 mg RTE / g extract, respectively while the TPC were recorded as follows: 103.79; 119.47; 110.10 mg GAE / g extract. Hence, 70% ethanol were higher among others, and therefore remains as optimum solvent for the extraction of A. bilimbi leaves.Â

An interventional study on the effectiveness of peer assistance for medication adherence among hypertensive patients in Purwokerto Hening Pratiwi; Laksmi Maharani; Ika Mustikaningtias; Eling Bunga Nurani
Pharmaciana Vol 9, No 1 (2019): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Hypertension is a disorder of the blood vessels that hampers the transport of supply of oxygen and nutrients to the bodyâ€™s tissues. Antihypertensive therapy lasts a lifetime and, so, the success of hypertension treatment strongly depends on the willingness of patients to take antihypertensives regularly. Since medical non-compliance can adversely affect the patient's health, medication supervision or peer assistance has been proposed as a way to monitor and remind hypertensive patients to adhere to prescribed daily dosage. This study aimed to determine the effect of peer assistance on adherence to hypertensive medication. It employed a quasi-experimental model with a one-group pretest-posttest design and a peer-based mentoring to achieve drug compliance as the intervention. The research samples were patients registered in the Chronic Disease Management Program (Program Pengelolaan Penyakit Kronis) at two primary health services in Purwokerto. In the first four weeks, a total of 29 respondents acted as the control group without receiving any intervention, and in the second four weeks, they became the intervention group who received peer assistance for their regular drug intake. To measure compliance, the Hill-Bone questionnaire was used. The expected maximum compliance score is 56. The results showed that the average score of compliance increased from 47.69 in the pre-control period to 49 in post-control/pre-intervention and then to 49.93 in post-intervention. The compliances during the control and intervention period had similarly significant differences with p values of 0.008 and 0.039, respectively. In conclusion, peer assistance does not affect patientsâ€™ adherence to hypertension treatment.

Antibacterial activity of extracts and fractions of wood ear mushroom (Auricularia auricula) Ika Kurnia Sukmawati; Elis Susilawati; Sindi Dwi Putri
Pharmaciana Vol 9, No 1 (2019): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Infection is a disease caused by pathogenic bacteria, such as Staphylococcus aureus, Escherichia coli, Bacillus cereus, and Pseudomonas aeruginosa. The wood ear mushroom (Auricularia auricula) has been empirically and scientifically proven as an agent that can treat infections because it contains antimicrobial properties like polysaccharide and protein complex. This study aimed to determine the antimicrobial activity of the A. auricula extracts and fractions. The first stage of the test comprised the collection, determination, and processing of the test plant. The simplicia was characterized by its water content, ash content, acid solubility, and total ash content. The next step was reflux extraction using 96% ethanol. After a concentrated extract was formed, it was subjected to phytochemical screening and fractionation using liquid-liquid extraction (LLE) with n-hexane, ethyl acetate, and methanol-water solvents. Each of the A. auricula extracts and fractions was tested for antimicrobial activity. Microdilution method was employed to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC), which were later compared with the antibiotic properties of tetracycline. Scanning Electron Microscopy (SEM) was used to observe any morphological changes in the tested bacteria. The MICs of the A. auricula extract, n-hexane fraction, and ethyl acetate fraction against S. aureus were 256 Î¼g/mL, 128 Î¼g/mL, and 64 Î¼g/mL, respectively. However, the bactericidal effects of the test mushroom were not achieved in the experiment. Based on the MICs, the ethyl acetate fraction has the best inhibitory activity. Moreover, the bioautography test of this fraction produced Rf value= 0.78, implying the formation of a zone of growth inhibition. The SEM of the A. auricula extracts and fractions proved that this mushroom altered the morphology of S. aureus bacteriaâ€”i.e., the cells became rounded and tapered and showed indications of cell shrinkage and damage. As a conclusion, the ethyl acetate fraction of A. auricula has the best antimicrobial activity against S. aureus.

In vitro penetration of alpha arbutin niosome span 60 system in gel preparation Rise Desnita; Sri Luliana; Silvana Anggraini
Pharmaciana Vol 7, No 2 (2017): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Alpha arbutin is a hydrophilic compound which is difficult to pass trough the stratum corneum. One of the effort to increase the penetration of the drug through the stratum corneum is by making in niosome system. This study aims to determine the optimum concentration of span 60 to improve the entrapment efficiency of niosome and investigate the increasing penetration of alpha arbutin using the niosome system in the preparation of the gel in vitro. Niosome consist a mixture of span 60 and cholesterol it made by thin layer hydration method. Concentration of span 60 was varied into three formulas were 100, 150 and 200 ÂµmoL. The formulation of gel was made in two formulas including formulation of niosome alpha arbutin and alpha arbutin in gel. The determination of enterapment efficiency showed that formula 100Âµmol has an optimum enterapment efficiency by 99.09%Â±0.1. The in vitro penetration tests with shed snake skin membrane showed that usage span 60 as a niosome composer could increase penetration of alpha arbutin in gel formulation with cumulative numbers of diffusion in 8 hours was 91.62%Â±2.32 compared to alpha arbutin in gel without niosome system about 73.00%Â±0.94.7.

Page 30 of 81 | Total Record : 808

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Editorial Address Jl. Prof. Dr. Soepomo, S.H., Janturan, Warungboto, Umbulharjo, Yogyakarta, Indonesia Kode pos 55164

Location Kota yogyakarta, Daerah istimewa yogyakarta INDONESIA

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Contact Name
Muhammad Syahrir

Contact Email
m.syahrir7406@unm.ac.id

Phone
-

Journal Mail Official
nurkhasanah@pharm.uad.ac.id

Editorial Address
Jl. Prof. Dr. Soepomo, S.H., Janturan, Warungboto, Umbulharjo, Yogyakarta, Indonesia Kode pos 55164

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA