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INDONESIA
Majalah Kedokteran Gigi Indonesia
Published by Universitas Gadjah Mada
ISSN : 24600164     EISSN : 24422576     DOI : https://doi.org/10.22146/majkedgiind.36959
Core Subject : Health,
Dentistry
Arjuna Subject : -
Articles 575 Documents
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Relationship between peatland water use and periodontal disease in Daha Selatan Subdistrict Hidayah, Antung Nurul; Oktiani, Beta Widya; Azizah, Aulia
Majalah Kedokteran Gigi Indonesia Vol 8, No 3 (2022): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.76843

Abstract

Periodontal disease is one of the dental and oral health problems in Hulu Sungai Selatan Regency with a prevalence of gingivitis of 24.58% and periodontitis of 10.83%. One of the risk factors for periodontal disease is the use of peatland water. Peatland water has a low pH which can affect the colony of anaerobic bacteria in the oral cavity of the water users. Bacteria that cause periodontal disease are able to develop and survive in acidic conditions. This study aimed to analyze the relationship between peatland water use and periodontal disease in Daha SelatanSubdistrict. This was analytical research with a cross-sectional approach. The sampling technique used a simple random sampling technique with a total sample of 100 people (n = 100) who were residents aged 35-44 years in Daha Selatan Subdistrict. The results showed that the participants with high use of peatland water showedthe highest percentage (39%). In addition, there were 61% respondents who suffered from periodontal disease. ChiSquare test obtained a significance value of 0.022 (p < 0.05), showing that there was a relationship between peatland water use and periodontal disease. The risk of periodontal disease in the respondents who used peatland water in the high category was 4.167 times greater than in the respondents who did not use peatland water. It is essential to educate the general public about the effects that using peatland water can have on dental and oral health because there is a high prevalence of periodontal disease, which is caused by the widespread use of peatland water.
Treatment of temporomandibular joint trauma following odontectomy Ruspita, Intan; Dipoyono, Haryo Mustiko
Majalah Kedokteran Gigi Indonesia Vol 8, No 3 (2022): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.78415

Abstract

Temporomandibular Joint Disorder (TMD) is a non-dental musculoskeletal disorder that has a complex and multifactorial etiology. The most frequent symptoms are pain in the masticatory muscles, in front of the ears, and in the temporomandibular joint (TMJ); this disorder can also be accompanied by clicking sounds and locking. This study aimed to present a case report of temporomandibular joint trauma following odontectomy. A 22-year-old patient came with some complaints, namely pain in the TMJ, a clicking sound when opening and closing the mouth, and limited mouth opening. The symptoms started when the patient underwent odontectomy on all the four third molars, causing tightness around the jaw, pain, and clicking. However, she did not seek any treatment for these conditions until the pain became worse and disturbed her activities. The patient had to deal with limited mouth opening and severe migraines up to twice a day. During an examination, the patient was able to open her mouth without pain only 25 mm in width, with a maximum mouth opening of 35 mm. The therapy was done by constructing a 4-mm stabilization splint and educating the patient on how to reduce the pain. In fact, 2 months after the treatment and the use of stabilization splints, her complaints of TMJ pain and clicking sounds decreased, her mouth opening increased, and the migraines never came back. In this case, non-invasive treatment as the first-line therapy in patients with disc displacement with reduction and arthralgia can reduce the joint pain symptoms, and some of the symptoms have even disappeared.
The relationship between bizygomatic width and mesiodistal width of six maxillary anterior teeth in a Javanese population Indrastuti, Murti; Barunawati, Sri Budi; Hasudungan, Dian Samuel
Majalah Kedokteran Gigi Indonesia Vol 8, No 3 (2022): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.79146

Abstract

The selection of the mesiodistal width of maxillary anterior teeth is the key to success in prosthodontic treatment. Determining anterior teeth width when there is no existing pre-extraction record can be done through bizygomatic width measurement. The purpose of this study was to look at the relationship between bizygomatic width and mesiodistal width of six maxillary anterior teeth in the Javanese population. This study used a cross-sectional design and was conducted on 32 Javanese subjects who met the following criteria: being 18-25 years of age, having Angle class 1 malocclusion, having complete maxillary anterior permanent teeth, not using dentures, currently not undergoing any orthodontic treatment, and having no abnormalities found in the facial area. The bizygomatic width was measured with a digital caliper with an accuracy of 0.01 mm from the marked right and left bizygomatic points. The mesiodistal width of the six maxillary anterior teeth was measured using dental floss looped from the distal left canine to the right canine. Dental floss marked with a pen was stretched horizontally on paper and then measured with a digital caliper with an accuracy of 0.01 mm. The measurement was done in triplicate. Data were analyzed using Pearson’s correlation and the simple linear regression test. This study’s analysis revealed a strong and positive correlation (r = 0.538 and p < 0.05) between the bizygomatic width and mesiodistal width of the maxillary anterior teeth. The result of the simple linear regression test showed a significant result (p < 0.05). It can be concluded that there was a relationship between the bizygomatic width and the mesiodistal width of the six maxillary anterior teeth in the Javanese population. The mesiodistal width of the six maxillary anterior teeth in the Javanese population can be measured by multiplying the bizygomatic width by the multiplier factor, i.e., 0.427.
Differences in Candida Albicans hydrophobicity and biofilm formation between thermoplastic nylon and polyether-ether-ketone denture base materials Ismiyati, Titik
Majalah Kedokteran Gigi Indonesia Vol 8, No 3 (2022): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.79300

Abstract

Polyetheretherketone (PEEK) and thermoplastics nylon are extensively composed as an alternative material for removable denture base. The primary difference between the two materials is associated with water absorption properties. The two materials interact with saliva and microorganisms during application in the oral cavity. Candida is predominantly reported in the cavity. The microorganism is distinguished by its hydrophobicity and ability to form biofilms. The objective of this study was to determine the differences between thermoplastic nylon and polyether-ether-ketone as removable denture materials in terms of hydrophobicity and Candida albicans biofilms. It is a laboratory experiment using 20 5 x 2 mm samples for the hydrophobicity test and Candida albicans biofilm. The hydrophobicity test was performed by determining the contact angle with a Goniometer and the WinDrop++ software. The biofilm test samples were divided into 4 groups, encompassing PEG 5% (negative control), PEEK treatment, thermoplastics nylon, and 0.12% chlorhexidine (control positive). The microdilution method was employed to examine biofilm formation. The results of the study were interpreted, and the data were analyzed by employing the t test. The research discovered significant differences in biofilm formation, large contact angle on PEEK and thermoplastics nylon (p < 0.05). Biofilm formation on thermoplastics is significantly larger than PEEK (p < 0.05). In conclusion, the contact angles of PEEK and thermoplastic nylon are incorporated in the partially wetted. Polyetheretherketone is involved in the non-producers of biofilms while thermoplastic nylon is categorized in of weak biofilm formers.
The concentration effect of kulim leaf (scorodocarpus borneensis) extract on Streptococcus mutans ATCC 25175 bacterial hydrophobicity and adhesion Utami, Trianna Wahyu; Hapsari, Adhaninggar Ratna; Hanalda, Dhe Rifdania; Nur, Asikin; Yulianto, Heribertus Dedy Kusuma; Purwanti, Nunuk
Majalah Kedokteran Gigi Indonesia Vol 8, No 3 (2022): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.80636

Abstract

With the ability to reduce hydrophobicity and inhibit the adhesion of S. mutans ATCC 25175 bacteria, Kulim leaf extract can be used as an alternative to chlorhexidine mouthwash in caries prevention. The objectives of this study are to determine the effect of Kulim leaf extract on hydrophobicity and adherence of the cariogenic bacteria S. mutans ATCC 25175. The test groups were divided into negative control (DMSO 2%), the treatment group (Kulim leaf extractconcentrations of 1.25%, 2.5%, 5%), and positive control (0.1% chlorhexidine). All of groups were received three replicated tests for hydrophobicity and adherence inhibition of S. mutans ATCC 25175 bacteria. The hydrophobicity test was conducted by providing 3 ml of bacterial suspension of S. mutans ATCC 25175 which had been adjusted to the McFarland 0.5 standard for each group. Furthermore, each test group was vortexed for one minute and left to stand for 15 minutes. Each treatment was tested with a wavelength spectrophotometer of 550 nm before and after the provision of 200 µl of n-hexadecane. The absorbance value on the spectrophotometer was then included in the hydrophobicity formula to determine the hydrophobicity percentage of S. mutans ATCC 25175 against n-hexadecane. To test the bacterial adhesion, the 96 wells microplate was inserted with the kulim leaf extract of each concentration,BHI-B, bacteria according to the McFarland 0.5 standard, and for the positive control and negative control. Afterwards, they were incubated at 37 °C for 24 hours before they were rinsed with distilled water, and stained with 0.1% crystal violet. Then, an optical density reading was performed using a microplate reader with a wavelength of 540 nm. The absorbance value was then included in the formula for percentage of bacterial adhesion inhibition. Post-Hoc LSD testshowed a significant difference in mean difference between the negative control group and the other treatment groups (p<0.05). In addition, it was revealed that there was no significant mean difference between treatment groups, and there was no significant difference between positive control and treatment groups of 2.5% and 5% in the hydrophobicity test. However, there was a significant difference between the positive control and the treatment group of 1.25% in the hydrophobicity test and the treatment group of 1.25%, 2.5%, and 5% in the adherence test. This study concluded that Kulim leaf extract concentration affected hydrophobicity and attachment of S. mutans ATCC 25175 with an effective concentration of 2.5%.
A potential of Jasminum sambac (L.) Aiton leaf nano-extract as spray treatment of gingivitis-induced Sprague Dawley rats Nuryanti, Archadian; Larasati, Puspa Ayu Aprilia; Almeyda, Alda Risma; Marandof, Monica Agnesia; Irawan, Catherine Marcia
Majalah Kedokteran Gigi Indonesia Vol 9, No 1 (2023): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.69468

Abstract

Gingivitis is the second most common disease that occurs in the oral cavity. The use of chlorhexidine as a gingivitis therapy agent has some side effects such as allergic reactions and bacterial resistance. Jasminum Sambac (L.) Aiton leaf nano-extract contains flavonoids, saponins, and tannins compounds reported to have an influence on wound healing process. The aim of this research was to determine the effect of observation time and concentrations of Jasminum sambac (L.) Aiton leaf nano-extract on the number of neutrophils, macrophages, and angiogenesis in the treatment of gingivitis-induced Sprague Dawley rats. Sixty male Sprague Dawley rats aged 2.5-3 months with body weight of 200-250 g were anesthetized with ketamine HCl (10 mg/kg BW) and xylazine (2 mg/kg BW) then induced using silk ligature 3.0 on the interdental of the mandibular incisor. The rats were divided into five groups and got daily spray using distilled water (negative control), nano-extract at a concentration of 40%, 45%, 50%, and 0.12% chlorhexidine (positive control). The rats were euthanized on the 1st, 3rd, 5th, and 7th days post-treatment. The tissues were processed histologically with HE staining. The number of neutrophils, macrophages, and angiogenesis were counted using a microscope (400x) and OptiLab Viewer® (13x) at five fields of view. The result of the Two Way ANOVA test showed that there were significant effect of concentrations and observation time, and interaction of both observation time and concentrations on the number of neutrophils, macrophages, and angiogenesis (p < 0.05). It can be concluded that observation time and concentrations of Jasminum sambac (L.) Aiton leaf nano-extract affected the number of neutrophils, macrophage, and angiogenesis in the treatment of gingivitis-induced Sprague Dawley rat (p < 0.05).
Prevalence and identification of oral candida species in patients with type 2 diabetes in Yogyakarta Agustina, Dewi; Chrisnawati, Regina Titi; Chrismawaty, Bernadetta Esti; Wongsohardjono, Sri Budiarti; Naritasari, Fimma; Sarasati, Andari
Majalah Kedokteran Gigi Indonesia Vol 9, No 1 (2023): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.76176

Abstract

Patients with diabetes are prone to recurring and even resistant Candidiasis, making treatment challenging. Many hypotheses proposed related to susceptibility of diabetic patients to Candida. The prevalence and species of Candida in a particular diabetic community might be different compared to other diabetic community. This study aimed to determine the prevalence of Candida colony and its species in the oral cavity of diabetic patients included in the CDM (Chronic Disease Management) program in Yogyakarta. One hundred patients with type 2 diabetes (n= 100) were recruited as the subjects of this study. The subjects were classified into controlled and uncontrolled Diabetes mellitus (DM). Samples of oral rinse solution were collected to determine the species of Candida and number of Candida colonies using CHROMagar Candida medium. There were 47 and 53 of subjects with controlled and uncontrolled DM, respectively. The mean number of Candida colony in the subjects with controlled diabetes (1003.13) was higher than that in the subjects with uncontrolled diabetes (478.43). The Candida colony most commonly identified in the subjects with controlled and uncontrolled diabetes were C. albicans and C. glabrata, respectively. Female patients had higher mean number of Candida colony (859.51) compared to male (299.21). The Candida colony most often identified in both genders was C. albicans. In addition, the subjects of this study consisted of 83 geriatric subjects and 17 non geriatric subjects, in which the mean number of Candida colony in the geriatric subjects (761.77) was higher than that in the non-geriatric subjects (545.71). The Candida colony most often identified in the geriatric subjects and non-geriatric subjects was C. albicans and C. glabrata, respectively. The Mann Whitney test demonstrated that there was a significant difference (p = 0.009) of the mean number of Candida colony between male and female. However, there was no significant difference of the mean number of Candida colony between ages (p = 0.060) and diabetic status (p=0.175). It can be concluded that the Candida species most commonly identified in all the subjects was Candida albicans with the mean colony number of 349.96, followed by C. glabrata (225.97), C. krusei (144.91), C. tropicalis (3.67), and other species (2.02).
An Inhibition effect of immersion in effervescent garlic ethanol extract (Allium sativum L.) against Staphylococcus aureus growth on heat cured acrylic Ramadhanti, Dian Praba; Rachmani, Eka Prasasti Nur; Kurniawan, Aris Aji
Majalah Kedokteran Gigi Indonesia Vol 9, No 1 (2023): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.77132

Abstract

Denture stomatitis is an infection of the mucosa caused by bacteria such as Staphylococcus aureus (S. aureus) accumulating on the denture. Garlic (Allium sativum) contains antibacterial compounds that can be used as an alternative denture cleanser. The purpose of this study was to determine the inhibition effect of immersion in effervescent garlic ethanol extract (Allium sativum) against Staphylococcus aureus growth on heat cured acrylic dental plate. This research was a laboratory experiment in vitro using 40%, 50%, and 60% effervescent garlic ethanol extract. The samples were 12 pieces (n = 12) of heat cured acrylic plates which were divided into 3 treatment groups then incubated in S. aureus suspension for 24 hours and then immersed in an effervescent garlic ethanol extract for 6 hours. Bacterial colonies were counted using a colony counter and the data were analyzed using the One-way ANOVA and LSD Post hoc tests. The statistical analysis showed that the number of S. aureus colonies decreased along with an increased concentration of garlic ethanol extract. The results of the analysis showed that the 60% effervescent garlic ethanol extract had a significantly lower mean number of colonies compared to the 40% and 50% effervescent garlic ethanol extracts (p ≤ 0.05). This research concluded that effervescent garlic ethanol extract prevented the growth of S. aureus on the heat cured acrylic dental plate.
A histological evaluation of cellular response on bone regeneration scaffold Dewi, Anne Handrini; Listyarifah, Dyah; Ardhani, Retno; Jansen, John
Majalah Kedokteran Gigi Indonesia Vol 9, No 1 (2023): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.77449

Abstract

Biocompatibility testing for a new implant material is necessary before clinical use. The materials for bone scaffolding must also go through biocompatibility tests both in vitro and in vivo. The purpose of this study was to compare different staining methods in histological preparations to assess the biocompatibility of the material to tissues. Twenty Wistar rats (n= 20), aged 4 months, males, weighing about 250-350 g were divided into 2 research groups, namely sub-cutaneous and bone implantation for 3, 14, and 30 days. The sample was a biodegradable material with gypsum and calcium carbonate as the basic ingredients. Histological stains were prepared in several ways using Hematoxylin Eosin (HE), Mallory, Toluidine Blue, Tartrate-resistant acid phosphatase polyclonal antibody (TRAP), and Chromogen 3,3 diaminobenzidine (DAB) after pre-performing a decalcification process with ethylenedinitril-o-tetra-acetic acid (EDTA). The observation was done using magnification ranging from 10X10 to 100X10 with a light microscope (Nikon eclipse E600) and OptiLab viewer. The results were displayed in the form of descriptions of images generated from different staining methods. Staining by the HE method is most often chosen because it is cheap and easy but the other staining methods such as Toluidine Blue, Mallory, and TRAP show better contrast. Tissue staining techniques with immunohistochemistry (DAB, VEGF) are used to evaluate cell and tissue responses by identifying specific proteins present.
Effect of aloe vera on the proliferation phase of oral mucosal wound healing in rats Primasari, Ameta; Yendriwati, Yendriwati; Minasari, Minasari; Buaya, Erlin Kristiani; Madani, Sri Ayu Winda; Sembiring, Kartika Nabila; Hidayati, Rizka
Majalah Kedokteran Gigi Indonesia Vol 9, No 1 (2023): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.77682

Abstract

Aloe vera is known as a medicinal plant containing an active substance acemannan thought to play a role in accelerating wound healing. This study aims to determine the effect of aloe vera extract on the proliferation phase of oral mucosa wound healing in rats by looking at epithelial thickening, epithelial gaps closure, growth of new blood vessels, and connective tissue growth. This research is a pure experimental study with a post-test only control group design. On the left buccal mucosa of the mouth of 24 white Wistar rats (n = 24), an incision was made by scalpel with 10 mm length and 2.5 mm depth. The rats were divided into 4 groups: groups 1 and 2 as controls, while groups 3 and 4 were applied with aloe vera extract made by maceration technique. Wound tissue was observed histologically with Hematoxylin-Eosin staining under a microscope with an image analyzer for measuring the thickness of epithelium. The analysis of epithelial gap width used morphometry, and the number of capillaries was counted manually. Connective tissue thickness was measured with Image-J software. These were carried out on days 7 and 14 after treatment and the data were analyzed with the independent t-test and Mann-Whitney test. The results showed that aloe vera extract had no significant effect on epithelial thickening on day 7 (p = 0.701) nor on day 14 (p = 0.639), but a significant effect was shown on epithelial gaps closing (p = 0.049), connective tissue thickening (p = 0.004), and capillary increase on day 7 (p = 0.049). It could be concluded that aloe vera extract could potentially accelerate the epithelial gaps closure, connective tissue thickening, and capillary increase during the proliferation phase of the oral mucosa wound healing.

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