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Bioeksperimen: Jurnal Penelitian Biologi
Published by Universitas Muhammadiyah Surakarta
ISSN : -     EISSN : -     DOI : -
Core Subject : Education, Agriculture,
Agriculture, Biological Sciences & Forestry Education
Arjuna Subject : -
Articles 484 Documents
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Composition and Zonation of Mangrove Vegetation in Ujung Pangkah, Gresik, Indonesia Mabruroh, Arofatul Syabina; Firdhausi, Nirmala Fitria; Bahri, Saiful; Rachmawati, Yuanita; Munir, Misbakhul
Bioeksperimen: Jurnal Penelitian Biologi Vol 10, No 2: September 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v10i2.23819

Abstract

Ujung Pangkah Gresik is a significant mangrove area with both ecological and economic functions. Ecologically, it prevents seawater intrusion and abrasion, while economically, it supports sectors like tourism, forestry, fisheries, and ecotourism. This research aims to identify mangrove species diversity and zoning patterns in Ujung Pangkah using survey methods to assess diversity, density, dominance, importance value, and zoning. Four species were found (Avicennia marina, Avicennia officinalis, Rhizophora stylosa, Ceriops tagal) with 454 individuals. The diversity value is moderate at 1.86. The highest and lowest densities were 0.52 and 0.01, respectively. Avicennia marina is the dominant species with an importance index of 273.69. The area has a mixed zoning pattern.
Studi Penerapan Bank Sampah dalam Upaya Pengelolaan Lingkungan Hidup di Kota Yogyakarta Haryanti, Sri; Gravitiani, Evi; Wijaya, Mahendra
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 1: March 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i1.10434

Abstract

Produk Lipase Kapang Lipolitik pada Limbah Ampas Kelapa Suyanto, Eko; Soetarto, Endang Sutariningsih; Cahyanto, Muhammad Nur
Bioeksperimen: Jurnal Penelitian Biologi Vol 1, No 1: March 2015
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v1i1.311

Abstract

Lipase memiliki manfaat penting di bidang industri. Tujuan penelitian ini adalah untuk mendapatkan kapang lipolitik yang mampu tumbuh dan menghasilkan aktivitas lipase tinggi pada limbah ampas kelapa menggunakan metode solid state fermentation. Isolat kapang uji dipurifikasi kemudian dilakukan skrining dan seleksi kapang lipolitik dan dilanjutkan dengan produksi lipase menggunakan substrat ampas kelapa yang sebelumnya diukur kandungan biokimia. Hasil menunjukkan bahwa 8 isolat kapang lipolitik mampu tumbuh baik pada substrat ampas kelapa yang ditunjukkan dengan adanya sporulasi dan perubahan pH medium selama reaksi. Diantara kapang lipolitik tersebut, isolat kapang KLC-333 diketahui menghasilkan aktivitas hidrolisis lipase terbesar yaitu 13,33 U/ml dan volume produksi 46 ml. Biosintesis dan peningkatan produksi lipase dipengaruhi oleh kandungan nutrien di dalam substrat ampas kelapa.
Ophiocordyceps : Fungi Entomopatogen Penyebab Zombie Pada Serangga Putra, Ivan Permana
Bioeksperimen: Jurnal Penelitian Biologi Vol 7, No 2: September 2021
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v7i2.11152

Abstract

Ophiocordyceps is special type of entomopathogenic fungi which well known to infect insects and turning them into zombie. A total of 250 species have been described from its genus, and some are known to control their hosts, which are most notably ants. They are ubiquitous within tropical rain forests across the board, with few publication reports from temperate ecosystems. These fungal pathogens possess the capacity to manipulate host behaviour in order to increase their own fitness. These entomopathogenic fungi cause their hosts frezee to die in an exposed area on the abaxial surface of leaves or branches, which then enhancing the fungus spore production and dispersal. Despite the high diversity of fungi and insect with vast tropical forest as well as drugs potency from Ophiocordyceps, publication of Ophiocordyces was rarely reported from Indonesia. This review will explain the evolution of Ophiocordyceps-insect interaction, simbiont distribution, and zombie-insect formation mechanism, in order to induce zombie-insect research in Indonesia. 
PENGARUH PEMBERIAN GIBERELIN DAN AIR KELAPA TERHADAP PERKECAMBAHAN BIJI ANGGREK BULAN (Phalaenopsis sp.) Mukminin, Lilik Hidayatul; Al Asna, Putri Moortiyani; Setiowati, Frida Kunti
Bioeksperimen: Jurnal Penelitian Biologi Vol 2, No 2: September 2016
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v2i2.2487

Abstract

Phalaenopsis sp. atau dikenal dengan nama dagang anggrek bulan termasuk famili Orchidaceae yang memiliki nilai ekonomis tinggi. Pembudidayaan tanaman anggrek selama ini terkendala pada biji anggrek yang memiliki daya kecambah kurang dari 1%. Daya kecambah biji yang rendah disebabkan oleh ukuran biji yang kecil dan tidak mempunyai endosperm. Oleh karena itu, perkecambahan biji anggrek perlu didukung oleh hormon tumbuh yang sesuai. Giberelin dan air kelapa diketahui dapat berperan dalam perkecambahan biji. Tujuan dari penelitian ini untuk mengetahui pengaruh pemberian Giberelin (GA3) dan air kelapa terhadap perkecambahan biji anggrek bulan (Phalaenopsis sp). Tahapan penelitian yang dilakukan yaitu melakukan sterilisasi, kemudian biji Anggrek diinokulasikan dalam medium MS dengan perlakuan variasi konsentrasi GA3 1 ppm, 2 ppm, 3 ppm dan perlakuan variasi konsentrasi air kelapa 10 mL, 20 mL, dan 25 mL beserta kombinasinya kemudian diamati pada hari ke-21. Parameter yang diamati berupa perkembangan morfologi protocorm dan fase perkembangan protocorm yang dianalisis secara deskriptif, sedangkan daya kecambah protocorm dianalisis ANOVA dan dilanjutkan uji Tukey pada tingkat kepercayaan 95% untuk melihat adanya perbedaan antarperlakuan. Perkembangan protocorm pada 3 perlakuan memperlihatkan fase perkembangan protocorm yang berbeda, yaitu embrio membengkak dan merobek testa, protocorm putih dengan absorbing hair, protocorm putih kekuningan, dan protocorm hijau bulat. Hasil penelitian menunjukkan bahwa pemberian giberelin (GA3) 3 ppm menghasilkan daya kecambah paling rendah dan pemberian kombinasi antara GA3 dan air kelapa menunjukan daya kecambah yang tidak berbeda nyata dengan perlakuan MS.
Paclobutrazol And Cytokinin Regulation On Culm Growht Of Black Rice (Oryza sativa L. “Cempo Ireng”) Darussalam, Darussalam; Dewi, Kumala
Bioeksperimen: Jurnal Penelitian Biologi Vol 8, No 2: September 2022
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v8i2.19111

Abstract

Rice is a staple food for the majority of Asians; nevertheless, black rice has not been commonly consumed among them. The components of the yield will be determined by the allocation of photosynthate on rice and the tall phenotype of black rice, which is a significant contributing factor to lodging. The growth inhibitor paclobutrazol prevents the manufacture of gibberellin, which results in the tall phenotype of black rice. The endogenous cytokinin content is high at the beginning of the grain filling and then rapidly decreases after that. The purpose of the study was to compare the effects of paclobutrazol and cytokinin on culm height, internode length, culm diameter, lodging resistance, culm parenchyma cell arrangement, and flag leaf structure. Throughout the inquiry, Cempo Ireng black rice cultivar seeds were employed. The seeds were planted in plastic containers that held 10 kg of soil for growth medium and organic fertilizer, and a random block pattern was used for the experiment's design. At 10 weeks following sowing, paclobutrazol was sprayed as a foliar application at concentrations of 0, 50, and 100 ppm. By injecting the kinetin diluted in 0.8 percent agar gel at a rate of 10-5 M in the flag leaf internode lacuna twice, separated by two days, two weeks after anthesis. Eight replications and six combinations were made. DMRT and the significant different were used in the statistical analysis of the mean comparisons (P=0.05). Plant height and internode length were decreased by paclobutrazol (100 ppm), although internode diameter was raised and lodging resistance was induced. The organization of the parenchyma cells in the culm (100 ppm packlobutrazol) changed, and the cells were small and lacked intercellular gaps.
KEANEKARAGAMAN KEPITING BIOLA DI KAWASAN MANGROVE KABUPATEN PURWOREJO, JAWA TENGAH Rahayu, Slamet Mardiyanto; Wiryanto, Wiryanto; Sunarto, Sunarto
Bioeksperimen: Jurnal Penelitian Biologi Vol 4, No 1: March 2018
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v4i1.5933

Abstract

Mangrove merupakan ekosistem yang khas dan memiliki fungsi penting secara ekologi, sosial-ekonomi, dan pendidikan. Luas kawasan mangrove di Kabupaten Purworejo semakin berkurang akibat adanya penebangan, permukiman, tambak,dan pertanian. Berkurangnya tegakan mangrove akan mempengaruhi keberadaan berbagai fauna yang berasosiasi dengannya. Oleh karena itu dilakukan pada bulan Agustus-September 2016 untuk mengetahui keanekaragaman kepiting biola di kawasan mangrove Kabupaten Purworejo, Jawa Tengah. Ada tiga stasiun, yaitu mangrove lebat (Desa Gedangan), mangrove sedang (Desa Jatikontal), dan mangrove jarang (Desa Ngentak). Dari penelitian didapatkan 7 jenis kepiting biola, yaitu Uca annulipes, U.crassipes, U.paradussumieri, U.rosea, U.tetragonon, U.vocans, dan U.vomeris. Indeks keanekaragaman kepiting biola di kawasan mangrove Kabupaten Purworejo, Jawa Tengah pada stasiun 1 (1,67)dan stasiun 2 (1,90) tergolong sedang. Adapun indeks keanekaragaman kepiting biola di stasiun 3 (0,64) tergolong rendah. Kondisi lingkungan di seluruh stasiun relatif baik untuk kehidupan mangrove dan kepiting biola, yaitu suhu26-30°C, pH 6-8, oksigen terlarut 3,5-6,6 mg/L, salinitas 3-9 ppt, dan substrat lumpur berpasir. Vegetasi mangrove pada stasiun I adalah Rhizophora mucronata, Nypa fruticans, Sonneratia alba, dan Hibiscus tiliaceus. Vegetasi mangrovepada stasiun II adalah Sonneratia caseolaris, Rhizophora stylosa, N.fruticans, H.tiliaceus, dan Morinda citrifolia.Vegetasi mangrove pada stasiun III adalah S.alba, S.caseolaris, N.fruticans, dan R.mucronata. Kerapatan vegetasi mangroveberkaitan dengan kelimpahan jenis (kepadatan) kepiting biola.
Toll-Like Receptor Activation and B Cell Maturation Via MyD88-Dependent Pathway Under Hyperglycemia Condition Aisyah, Riandini; Jatmiko, Safari Wahyu
Bioeksperimen: Jurnal Penelitian Biologi Vol 10, No 1: March 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v10i1.23328

Abstract

hyperglycemia causes a degenerative syndrome that involves an inflammatory process with an increase in certain proinflammatory cytokines and chemokines which in the process will activate B cells to produce immunoglobulins through several mechanisms. One of the interesting mechanisms is the mechanism via the MyD88 pathway. Objectives: to explore the role of MyD88 adapter protein in Toll-like receptor activation and B cell maturation under hyperglycemia conditions. Methods: a literature review was done to answer the study objectives. Results: Class switching process under hyperglycemia conditions involves activation of NFkB through the inflammatory MyD88-dependent pathway to trigger the expression of TLR and B cell maturation and proliferation as well as antibody production. The MyD88 adapter protein is a protein formed by stimulation of pro-inflammatory cytokines IL-6 and plays a role in the continuation of signals from the TLR and IL-1 pathways. Mature B cell stimulation induces 2 genetic changes in the Ig gene locus, called somatic hypermutation (SHM) and class switch recombination (CSR) to produce antibodies. Changes in immunoglobulin genes occur related to changes in certain DNA segments at the locus of genes where CSR occurs, this gene change requires the role of AID (activated-induced cytidine deaminase) in DNA cleavage. AID in mature B cells is activated by proinflammatory cytokines via induction of NfKB activation via the inflammatory MyD88-dependent pathway. Conclusion: Toll-like receptor activation plays a crucial role in B cell maturation activated by pro-inflammatory cytokine via MyD88 dependent-NFkB activation. 
Pengaruh Pasta Tomat Terhadap Kolesterol Darah Mencit Nugraheni, Febriani Sarwendah Asri; Jailani, Jailani; Purwati, Sri
Bioeksperimen: Jurnal Penelitian Biologi Vol 5, No 2: September 2019
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v5i2.9244

Abstract

This study aims to determine the effect of tomato paste on blood cholesterol in mice. It was an experimental study using a complete randomized study design consisting of four treatments P0, P1, P2, and P3 by giving tomato paste solution in a concentration of 0%, 20%, 40% and 80% and 6 replications. Data were analyzed using Anova one path and continued with BNT with a confidence level of 95%. The results showed that the tomato paste solution affected the blood cholesterol of mice due to Fhit (3.27) F tab (3.10) and the treatment that gave the greatest effect was on giving the concentration of tomato paste solution by 80%.
Optimalisasi Produksi Triterpenoid Dari Sangketan (Achyranthes aspera) Dengan Budidaya Organik Fanani, Zaenal; Farida, Umi; Nirwana, Muhammad Bayu
Bioeksperimen: Jurnal Penelitian Biologi Vol 7, No 1: March 2021
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v7i1.9482

Abstract

Kebutuhan akan bahan baku obat semakin meningkat sejalan dengan pemanfaatan obat tradisional yang semakin meningkat. Sangketan (Achyranthes aspera) adalah salah satu tanaman obat potensial, mengingat tanaman ini memiliki kandungan senyawa aktif yang bermanfaat untuk kesehatan. Triterpenoid merupakan salah satu kandungan metabolit sekunder utama dari Sangketan. Tujuan penelitian ini adalah untuk mengetahui kombinasi dari komposisi media tanam dan aplikasi fertigasi dengan pupuk organik, yang dapat mendukung produksi senyawa triterpenoid dari Sangketan secara optimal. Pada budidaya Sangketan diberikan perlakuan perbandingan komposisi media tanah + arang sekam padi 1:1; 1:2 dan 2:1. Sedangkan aplikasi fertigasi menggunakan pupuk organik kotoran kambing, dengan konsentrasi 1kg pupuk organik per 5 liter air, dosis 60 ml per tanaman dan diaplikasikan setiap dua minggu. Sangketan di panen setelah 4-5 bulan, kemudian dilakukan analisis kualitatif kandungan triterpenoid menggunakan metode kromatografi lapis tipis. Data yang diperoleh diaplikasikan menggunakan metode simplex lattice design (SLD), untuk memperoleh kombinasi yang optimal. Data kualitatif ekstrak Sangketan yang mengandungan senyawa triterpenoid, telah dianalisis dengan pereaksi kloroform dan H2SO4 pekat, ditandai dengan adanya lapisan warna coklat kemerahan. Data kuantitatif rendemen ekstrak Sangketan diaplikasikan menggunakan metode simplex lattice design, diperoleh persamaan Y = 8,94(Arang) + 11,585(Tanah) + 14,26(Arang.Tanah). Kandungan triterpenoid pada ekstrak Sangketan dibuktikan menggunakan kromatografi lapis tipis, berupa bercak warna abu-abu di bawah sinar UV 254 nm dengan nilai Rf 0,65.

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