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INDONESIA
METAMORFOSA Journal of Biological Sciences
Published by Universitas Udayana
ISSN : 23025697     EISSN : 26558122     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
METAMORFOSA is an electronic scientific journal published periodically by the Master of Biology Udayana University, which includes scientific works in the field of Biology.
Arjuna Subject : -
Articles 316 Documents
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Gambaran Histologi Lambung Dan Duodenum Mencit (Mus musculus L.) Jantan Yang Diberi Ekstrak Daun Kersen (Muntingia calabura L.) Setelah Diinduksi Monosodium Glutamat (MSG) Ni Wayan Adya Puttry Purna Yogini; Ngurah Intan Wiratmini; Ni Gusti Ayu Manik Ermayanti
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p02

Abstract

The antioxidant contained in kersen (Muntingia calabura L.) leaves has been known to be able to prevent animal tissue damages. Monosodium glutamate (MSG) is a food additive substance associated with histological damage of duodenum as well as increases secretion of HCl in stomach if consumed in excess amount. This study aimed to investigate the histological features of stomach and duodenum of monosodium glutamate-induced mice (M. musculus) after treated with kersen leaves extract, as well as establishing its optimum dose. This study used a Completely Randomized Design, with 25 male mice assigned to 5 treatment groups: 2 control groups i.e. negative control (K-) given aquades and positive control (K+) given 0.7mg/body weight MSG; and 3 intervention groups which were given kersen leaves extract as much as 0.075 mg/g bw (P1), 0.15 mg/g bw (P2) and 0.3 mg/g bw (P3). Both kersen leaves extract and MSG was administered orally once daily for 28 days. The parameters observed were histological features of stomach features (erosion of villi and inflammatory cells infiltration) and duodenum (erosion of villi, necrosis, and inflammatory cell infiltration). The results showed that all parameters observed in the aforementioned organs were significantly different (P<0.05) in intervention groups (P1, P2, dan P3) compared with positive control (K+). It is concluded that administration of kersen leaves extract improved the damages of stomach, duodenum, and liver of MSG-induced mice, and was best achieved at the dose of 0.3 mg/g bw.
Isolasi Bakteri Selulolitik Indigenous Pendegradasi Limbah Tandan Kosong Kelapa Sawit Agustinur Agustinur; Yusrizal Yusrizal
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p16

Abstract

The growth of palm oil industry increasing continuesly nowadays causes more waste produced from this commodity. One part of oil palm that is wasted and eventually becomes waste is oil palm empty fruit bunches (OPEFB). The largest component contained in OPEFB is cellulose. This component is very difficult to degrade because it is crystalline and insoluble in water. Several biological agents from the bacterial group are known to degrade cellulose because they have cellulolytic abilities. This study aims to isolate bacteria from OPEFB waste which has cellulolytic potential that can be used to degrade EFB waste optimally. The research stages began with sampling the decomposed OPEFB in the oil palm plantation area of ??PT Agro Sinergi Nusantara, Aceh Jaya Regency, Aceh. Then the bacterial isolation was carried out from the sample. The isolation results were 18 bacterial isolates. Furthermore, these isolates were purified and tested for the cellulolytic ability by inoculating on CMC media. The results of the cellulolytic ability test was 15 isolates had potency indicated of the formation of a clear zone around the colony. Among the 15 isolates, there were 4 bacterial isolates with high cellulolytic potency categories, namely isolates BK608, BK610, BK713 and BK818, with a cellulolytic index respectively 9.94; 6.67; 5.39 and 4.83.
Aktivitas Antifungi Saponin Bunga Kamboja Putih (Plumeria acuminata) pada Candida albicans ATCC 10231 Ni Kadek Yunita Sari; Ni Luh Utari Sumadewi
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p07

Abstract

Candida albicans is the main cause of candidiasis in Indonesia. Cases of infection due to Candida fungus have increased globally due to increasing cases of HIV / AIDS infection. White Cambodia is one type of plant that has various properties as a medicinal plant. So far several studies related to the effectiveness of natural antifungi have been carried out on the leaves of white frangipani plants. The effectiveness of antifungi in other white frangipani plant organs such as flowers is still rarely reported. The purpose of this study was to determine the saponin compound content of white frangipani flowers (Plumeria acuminata) and the effectiveness of these compounds on the growth of Candida albicans. The method used in this study: identification of antifungal saponin compounds was carried out by foam testing. Antifungal effectiveness test of saponin compounds on the growth of Candida albicans was tested by the Kirby-Bauer method. Quantitative data of inhibition zone diameters formed in each treatment were analyzed using ANOVA (Analysis of Variance) level of 5%. ANOVA analysis results were significantly different (P <0.05) followed by Duncan's test to see differences between treatments. The results showed that white frangipani saponins (Plumeria acuminata) had antifungal activity on Candida albicans as indicated by the emergence of a 7.7 mm inhibition zone (medium category). Keyword: Candida albicans, Acacia auriculiformis, antifungal, saponin
Cover & ToC Admin Metamorfosa
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

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Konsentrasi Logam Berat (Pb, Cu, Cd, Zn) pada Air dan Sedimen di Perairan Serangan, Bali Gede Surya Indrawan; I Nyoman Giri Putra
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p12

Abstract

Heavy metal pollution can disturb aquatic ecosystems and indicator of water quality. This study aims to determine the concentration of heavy metals (Pb, Cu, Cd, Zn) in water and sediments. This study was conducted in the waters of Serangan, South Denpasar District, Bali in June-August 2019. The research with each location taking 8 points of water and sediment sampling and measuring the quality of its waters. Water samples taken at the surface of the water as much as 330 ml and sediment samples taken as much as 500 grams. The method used is wet ashing preparation and analyzed by means of AAS (Atomic Absorbsion Spektrophotometri). The results of the average analysis of heavy metal concentrations in water are Pb 0 mg / L, Cd 0.438 mg / L, Cu 0 mg./L, and Zn 0.284 mg / L. The results of the analysis are compared with the standard quality value of sea water that the heavy metals Cd and Zn have exceeded the threshold. While the average concentration of heavy metals in sediments are Pb 17.444 mg / kg, Cd 18.093 mg / kg, Cu 113.981 mg / kg, and Zn 53.505 mg / kg. The concentration of Cd and Cu heavy metals exceeds the limits set by the ANZECC / ARMCANZ quality guidelines and the Canadian Council of Ministers of the Environment.
Uji Antioksidan Ekstrak Daun Tanaman Leunca (Solanum nigrum L.) Affrina Fauziah; Sang Ketut Sudirga; Ni Made Susun Parwanayoni
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p03

Abstract

Leunca plant (Solanum nigrum L.) is one of the plants that is often used as food for the people of Indonesia. Leunca plant leaves are widely used for herbal medicines. This study aims to determine the phytochemical content and antioxidant activity of leunca leaf extract (Solanum nigrum L.). The study was conducted at the Analytical Laboratory of Udayana University, the Organic Laboratory of Chemistry Study Programs at Udayana University and the Laboratory of the Faculty of Agriculture of Udayana University. Phytochemical tests were carried out to determine the presence of alkaloid compounds, steroids / triterpenoids, flavonoids, phenols and tannins. The antioxidant activity of leunca leaf extract (Solanum nigrum Linn) was tested by DPPH method (1-1-diphenyl-2-picrilhidrazil), with 5 different concentrations and consisted of 4 replications. Leunca positive leaf extract contains alkaloids, steroids / triterpenoids, flavonoids, phenols and tannins. The results showed that dried leunca leaf extract and fresh leunca leaf extract had different antioxidant activity. Dry leunca leaf extract is classified as strong antioxidant with antioxidant activity value of 64.40%, IC50 value of 95.12 ppm and fresh leunca leaf extract is classified as weak antioxidant with antioxidant activity value of 16.83%, IC50 value of 243.66 ppm. Keywords: Solanum nigrum L., herb, phytochemicals, antioxidant, DPPH
Profil Metabolit Daun Kesambi (Schleichera oleosa) Berdasarkan Analisis Histokimia dan In Silico Tintrim Rahayu; Radita Intan Aisyah Pratiwi; Nurul Jadid Mubarakati
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p17

Abstract

Kesambi (Schleichera oleosa) merupakan tumbuhan golongan familia Sapindaceae. Penelitian ini bertujuan untuk mengetahui senyawa metabolit sekunder pada daun kesambi melalui analisis histokimiadan in silico. Metode penelitian secara deskriptif eksperimental menggunakan sampel tumbuhan kesambi yang tumbuh di pulau Madura. Analisis histokimia dilakukan dengan preparasi daun segar melalui sayatan bawah daun dengan cara ditetesi reagen pendeteksi metabolit sekunder (CuSO4, FeCl3, Wagner, Sudan III, AlCl3 & FeCl3+NaCO3) kemudian diamati perubahan warnanya secara mikroskopis. Sedangkan pengujian in silico bertujuan untuk mengetahui interaksi senyawa aktif dengan ER? sebagai target terapi kanker payudara ER+ melalui molecular docking analysis. Web server pendukung yang digunakan yaitu KNApSAcK, Pubchem, Pass Online dan PDB ID serta software yaitu PyRx, PyMol dan Chimera 1.14. Hasil penelitian analisis histokimia menunjukkan bahwa daun kesambi mengandung senyawa metabolit sekunder yaitu terpenoid, flavonoid, alkaloid, tanin, lipofil dan fenol. Hal ini dikonfirmasi dengan analisis in silico yaitu didapatkan senyawa aktif seperti scopoletin yang merupakan turunan dari fenol, (-)-beta-sitosterol, betulin, betulinic acid, lupeol, lupeol asetat, schleicheol 1&2, schleicherastatin 1-7 yang merupakan turunan dari terpenoid. Berdasarkan hasil molecular docking terdapat interaksi senyawa aktif dengan protein 3ERT, senyawa yang memberikan hasil paling efektif sebagai kandidat obat kanker payudara yaitu lupeol asetat dengan nilai Root Mean Square Deviation (RMSD) paling baik yaitu l.b 1.588 Å dan u.b 2.219 Å. Senyawa lupeol asetat diprediksi memiliki aktivitas sebagai inhibitor ER? terhadap kanker payudara ER+. Kata Kunci : Kesambi (Schleichera oleosa), histokimia, molecular docking dan ER?
Isolasi dan Idetifikasi Bakteri aam Laktat Penghasil Bakteriosin dari Urutan, Sosis Fermentasi Tradisional Bali Ni Made Sri Dwijastuti; I Nengah Sujaya; Ni Nengah Dwi Fatmawati
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p08

Abstract

This study aimed to isolate and identify lactic acid bacteria (LAB) that could produce bacteriocins from urutan. Urutan is a Balinese sausage that is traditionally produced and naturally fermented. Urutans used in this study were obtained from several traders in the Baturiti, Mengwi, and Tegallalang region, Bali. More than 300 LAB colonies were isolated and underwent inhibition test against the growth of the indicator bacteria. Crude bacteriocins from two isolates, namely J2 and J6, showed inhibition zones against the growth of Lactobacillus plantarum E12.1 and Escherichia coli ATCC 8739. The J6 isolate also inhibited the growth of Enterococcus faecalis ATCC 29212. Both J2 and J6 were gram-positive, bacilli shaped, and showed negative catalase test. Based on the results of the 16S rDNA gene amplification, the two isolates were identified as Lactobacillus plantarum. Keywords: Lactic Acid Bacteria, Bacteriocin, Lactobacillus plantarum, Urutan
Pengaruh Suplemen Organik Terhadap Regenerasi Tunas Pisang Barangan Musa acuminata Colla. Secara In Vitro Mustika Tuwo; Baharuddin Baharuddin; Andi Ilham Latunra; A. Masniawati; Tutik Kuswinanti
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p13

Abstract

The research purpose to know ability of natural supplements in supporting the regeneration of banana cv. Barangan Musa acuminata Colla. Explants were cultured on MS basal medium (control) with natural supplements: coconut water (CW) 5; 10; 15 and 20%, green bean sprout extract (GBSE) and sweet corn extract (SCE) at 50; 100; 150 and 200 g/l. After 4 weeks of cultivation, maximum multiplication shoots (7.11 ± 0.19), shoot length (2.00 ± 0.22), and number of leaves (4.00 ± 0.38) was obtained in MS + 200 g/l bean sprout extract. The addition of natural supplements to the medium showed better results on all parameters of observation than control. This results indicates the potential of natural supplements for supporting of Barangan Musa acuminata Colla. propagation in vitro culture.
Suplementasi Soya Yogurt Untuk Perbaikan Kelimpahan Mikroba Sekum (Bakteri Asam Laktat Dan Escherichia coli) Tikus Diabetes Soya Yogurt Ni Wayan Desi Bintari; Putu Ayu Parwati
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p04

Abstract

Intestinal microbial dysbiosis is one of the triggers for metabolic syndrome. The increase in pathogenic bacteria triggers the high release of lipopolysaccharides which underlie the incidence of insulin resistance in diabetes mellitus. It is important to modulate the intestinal microbiota towards eubiosis through consumption of probiotics. A potential probiotic product is soy yogurt which contains live bacteria and high protein and isoflavones. The aim of this study was to examine the effect of soy yogurt supplementation on the abundance of cecum microbes (Lactic Acid Bacteria (LAB) and Escherichia coli) in diabetic rats. The research was conducted at the Laboratory of Stikes Wira Medika Bali. Type of research is experimental with a completely randomized design consit of 6 groups. The conditioning of diabetes mellitus was done by induction of nicotinamide (NA) 230 mg / kgBW and streptozotozin (STZ) 65 mg / kgBW. Abundance of cecum microbes analysis using Total Plate Count. The results showed soya yogurt supplementation in rat with diabetes mellitus had a positive correlation in improving the abundance of microbial cecum. The total abundance of LAB in diabetic rat was 7.36 ± 0.15 Log10 CFU/mL and was significantly different (p <0.05) compared to diabetic rat treated with soy yogurt supplementation, namely as much as 8.08 ± 0.1 Log10 CFU/ml. Meanwhile, the abundance of E. coli in diabetic rat was 9.42 ± 0.12 Log10 CFU/mL and was significantly different (p <0.05) compared to rats treated with soy yogurt supplementation, namely 8.14 Log10 CFU/ml.

Page 19 of 32 | Total Record : 316

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