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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
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Articles 12 Documents
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Search results for , issue "Vol 12, No 3 (2011)" : 12 Documents clear
Profil Kadar Kortisol dan Seng pada Kambing Peranakan Etawah Saat Melahirkan yang Diberi Tambahan Seng dalam Pakannya Sus Derthi Widhyari; Setyo Widodo; I Wayan Teguh Wibawan; I Ketut Sutama; Anita Esfandiari
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective at this experiment was to study the role of zinc supplementation (Zn) in minimizingstress at parturition. Thirty etawah cross breed age 3-6 years with average body weight 30-50 kg were usedin this study and divided into three groups ; i) animal were given grass + feed concentrate + ZnSO4 40 mg/kg dry matter ( control group) ; (ii) grass + feed concentrate + ZnSO4 60 mg/kg dry matter (treatment 1), and(iii) grass + feed concentrate + ZnSO4 80 mg/kg dry matter (treatment 2), respectively drinking water weregiven ad libitum. To monitor zinc and cortisone level, blood samples were withdrawn from each animalevery two weeks starting at age of pregnancy 3 months up to two months post parturition, in addition, tomonitor the cortisone level blood samples were also collected at 1,2,3 and 7 days post parturition. The Znand cortisone serum were analyzed using atomic absorbent spectro photometric (AAS) andradioimmunoassay (RIA), respectively the result showed that generally pregnancy at the beginning andget serum level at cortisone was relatively similar between the three animal groups and there was atendency to increase and reached peak at parts then gradually decrease until the and of the observation. Atparturition the lowest serum level at cortisone (19.00±18.72 mg/ml) was observed in animals receiving the60/ dry matter zinc supplement compared to animal receiving 80 mg/ dry matter zinc ( 52.65 ± 30.83 mg/ml) and control animal ( 75.92 ± 42.88 mg/ml). in addition serum level at Zn was significantly higher inanimal receiving 60 mg and 80 mg Zn in their diets compared to control animals. The best profiles wereseen in animal given 60 mg/kg dry matter Zn supplement. In conclusion, the addition of Zn in the diets hadsignificant effect in minimizing stress as pictured by the low serum level at cortisone at parturition.
Tepung Tempe Kaya Isoflavon Meningkatkan Kadar Kalsium, Posfor dan Estrogen Plasma Tikus Betina Normal I Nyoman Suarsana; I Nyoman Sadra Dharmawan; I Wayan Gorda; Bambang Pontjo Priosoeryanto4
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research aimed to study the effect of isoflavon-riched tempe flour on calcium (Ca, phosphate(P), and estrogen levels in plasma et normal female rats during their growth period. A level oftwenty-five 2 months old female Sprague Dawley rats with an avarage body weight of 200 g wasrandomly divided into into 5 groups: one with control group (KO: without treatment) and fourtreatment groups (K1, K2, K3, K4 : animals were given tempe flour with isoflavon at 1; 2; 4; and 6 mg/200 g/bw, respectively). The treatment was conducted for two months, following this blood plasmawas collected to analyse the level of calcium, phosphor, and estrogen, respectively. The resultshowed that although the plasma level of Ca, P, and estrogen was higher in the treatment groupcompare to the control group, this was not significantly different (P>0,05). The highest plasmalevel of Ca, P, and estrogen was seen in anmal receiving tempe flour with 4 mg/ 200 g bw/dayisoflavon.
Sel T Regulator CD4+CD25+ Mencegah Terjadinya Fenotip Letal pada Mencit Defisiensi CD122 Muhaimin Rifa’i; Widodo -
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Mice with a deficiency of cluster of differentiation/CD122 molecules experience increased memory Tcells. Increased memory T cells are activated and interfered with homeostasis that cause death at the ageof 10 ~ 12 weeks. To clarify whether the expression of CD25 molecules on CD4+ T cells responsible for thedevelopment of lethal phenotypes in CD122-/- mice, we did a transfusion of CD4+CD25+ T cells from normalmice to CD122-/- neonates. Transfusion of purified CD4+CD25+ T cells as much as 3 x 104 can prevent theoccurrence of lethal phenotypes generally experienced by CD122-/- mice. Transfusion of CD4+CD25+ T cellsin CD122-/- neonates cause all of the abnormalities that occur in T cell and leukocyte cells can be preventedand develop into normal. Similarly, the hematocrit that decreased dramatically in CD122-/- mice candevelop normally after receiving a transfusion of CD4+CD25+ T cells. In contras, transfusion of CD4+CD25-T cells in CD122-/- mice did not have the effect of preventing the development of the abnormalities inCD122-/- mice. CD4+CD25+ T cells that are lost in periphery of CD122-/- mice can restore to normal afterreceiving a transfusion of CD4+CD25+ T cells. These results clearly show that the expression of IL-2R?(CD25) on CD4+ T cells become pre-requisite for CD4 T cell population in order to play a role as regulatorcells.
Paparan Formalin Menghambat Proses Spermatogenesis pada Mencit Luh Gde Sri Surya Heryani; Ni Nyoman Werdi Susari; I Made Kardena; Desak Nyoman Dewi Indira Laksmi
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Formaldehyde is one of the exogenous free radicals. During formaldehyde exposure, there will be morefree radical or reactive oxygen compound formed through electron transport chain. Excessive reactiveoxygen compound triggers the process of lipid peroxide reaction in the membrane of spermatozoa cell. Theaims of this study were to find out that formaldehyde exposure caused abnormalities in the number of thenumber of spermatogonium type A, spermatogonium type Pachytene, 7th spermatid and 16th spermatid inspermatogenesis process.This study was experimentally and randomly pretested-posttested- with controlgroup design. The samples of this study were adult male mice strain Balb-C (age 2-3 months) with thefollowing criteria: body weight between 22-25 grams and healthy. Randomly, 48 mice were divided intothree groups, were control group, the first treatment group and the second treatment group. Prior to thetreatment, were taken a half from each group for the pre-test, by preparing microscopic preparation testicleand examination was performed to the total spermatogenic cells. The rests of the mice were used as posttestexamination after 35 days treatment. On the 36th day, all the rest mice were necropsied for microscopictesticle preparation. The result of this study showed that the formaldehyde exposure caused significantlydecrease in the number of spermatogenic cells (p<0,05), the average of type A spermatogonium cells atcontrol group, 1st treatment group and 2nd treatment group were 39,90±0,51; 20,42±0,72; 15,65±0,88respectively; spermatogonium type Pachytene were 48,47±1,28; 32,60±3,06; 23,14±3,16 respectively; 7thspermatid were 97,47±5,28; 39,98±4,28; 30,36±2,96 respectively and16th spermatid were 73,08±4,05;21,70±1,70; 16,38±1 respectively.It can be concluded that the formaldehyde exposure decreased the amountof spermatogenic cells. The result of this study is expected to be used as the baseline for further study inorder to measure formaldehyde content as a free radical in testicle .
Kebuntingan Hasil Transfer Blastosis Mencit yang Dibekukan dengan Metode Vitrifikasi Kriolup I Wayan Batan; I Ketut Suatha; Ita Djuwita; Nining Handhayani; Wahono Esti Prasetyaningtyas; Ketut Adnyane Mudite; Bibiana W Lay; Supar -; Arief Boediono
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the study was to assess the viability of vitrified embryo using cryoloop as a carrier ofembryo. The blastocyst stage embryos were collected from superovulated mice. Embryos were frozenusing vitrification method and vitrified embryos were loaded on copper filament cryoloop before dipped inliquid nitrogen. The viability of vitrified embryos was assess in vitro by medium cultered and in vivo bytransfered them to recipient mice. The result shows the viability of vitrified embryos was 85,7% after 24hours cultured and the embryos were born from two pregnant recipient mice out of nine (22%) or fouroffspring out of 63 trasfered embryos (6%). In conclusion, vitrified blatocyst stage embryos using cryoloopas a carrier could keep the viability of the embryos and they could be transfered to the recipient mice andwere born normally.
Efektivitas Ekstrak Bawang Putih terhadap Efek Hepatotoksik Aflatoksin B1 pada Ayam Pedaging Periode Awal Merry Muspita Dyah Utami; Ali Agus; Wihandoyo -; Kurniasih -
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to evaluate the effect of garlic extract (GE) in rations on the bloodcholesterol profiles of aflatoxin B-induced hepatotoxic in the early stage of broiler chickens. As many as140 one-day old chicks were used in this study. They were randomly divided into 16 treatment groups andeach group consists of 9 chickens. The experimental design adopted in this study was completely randomizedfactorial design consisting 4 concentrations of aflatoxin (A0 : 0 ppb, A1:500 ppb, A2: 1000 ppb and A3:1500 ppb) and 4 concentrations of garlic extract (B0: 0% B1:2% B2: 4% and B3 6%). The treatment wascarried out for 21 days. The serum levels of total cholesterol, low density lipoprotein (LDL) and highdensity lipoprotein (HDL), and HDL/LDL ratio were the examined. The result showed that Aflatoxin Bcaused hepatotoxicosis in broiler chickens indicated by the increase of total cholesterol and LDL, and thedecrease of HDL and HDL-LDL ratio. Treatment of chickens with 2% GE decreased total cholesterol andLDL level in serum, and increased HDL level and HDL-LDL ratio. No interaction effect was observedbetween aflatoxin B and GE extract treatments. It is evident that treatment of Garlic extract reduces thehepatotoxicosis effect of aflatoxin B treatment in broiler chickens.
Genetic Relationship Between Gembrong Goat, Kacang Goat and Kacang X Etawah Crossbred (PE) Based on Their Mitochondrial DNA I Gusti Lanang Oka; Wayan SayangYupardhi; Ida Bagus Mantra; Nyoman Suyasa; Anak Agung Sagung Dewi
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Gembrong goat is a specific type of goat which has long hair covers its whole body including its neckand face, originated from eastern part of Bali (Karangasem). A study of this type of goat and its relationshipwith other local goats (Kacang and Kacang x Etawah crossbred) was carried out at Sawe village, Jembrana,Bali.. A number of 12 gembrong goats, 3 kacang goats from Kubu village, Karangasem and 3 Kacang xEtawah crossbred goats from Denpasar were used in this study. Blood samples of all goats were collectedfor mitochondrial DNA (mtDNA) analysis. PCR amplification of D-loop mitochondrial DNA was carriedout by using two primers i.e. CAP-F (5’-CGTGTATGCAAGTACATTAC -3’) and CAP-R (5’-CTGATTAGTCATTAGTCCATC – 3’) . Sequencing of 550 bp (base-pairs) of mitochondrial DNA (productof PCR) only found one polymorphic site at base number 231 with two haplotypes in gembrong goat only,while the other base-pairs were similar between the three goat types (Gembrong, Kacang and Kacang xEtawah crossbred) .The frequency of haplotype 1 was 83.3% and the frequency of haplotype 2 was 16.7%.It was concluded that based on their mitochondrial DNA sequences and “phylogenic analysis”, the threetypes of goat (Gembrong,Kacang and Kacang xEtawah crossbred) had a very close genetic relationship(kinship).
Penampilan Reproduksi dan Perkembangan Skeleton Fetus Mencit Setelah Pemberian Ekstrak Buah Nanas Muda Iriani Setyawati
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This experiment was performed to examine the effects of young pineapple (Ananas comosus) fruitextract on reproduction performance and foetus skeletal development given during organogenesis period.Twenty pregnant mice were randomly divided into four groups. The young pineapple fruit extract wastreated orally by gavage with doses 0% (control), 20%, 40%, and 80% since gestation day 6 to 15. Caesareansection were performed to pregnant mice on day 18 to remove foetuses. Observation covered : reproductionperformance (uterine weight before and after foetus removal), the foetus number (reabsorptions, dead/alive foetus), morphological of the foetus (weight and length of the litter, and malformations), and skeletaldevelopment (ossification number of metacarpus, metatarsus, vertebrae caudalis) and malformations ofcostae, sternebrae, and vertebrae. Statistical analysis was performed using Anova and Duncan’s MultipleRange Test.Teratogenic effects caused decreasing of earlier uterine weight and alive foetus, increasingdead foetus and hemorrhage, delayed skeletal ossification (decreasing ossification number of metacarpusand metatarsus) and caused costal malformation (intercostal fusion and convulated costal structure).Young pineapple fruit extract given to pregnant mice during organogenesis alter reproductive performancepregnant mice. The extract also caused morphological abnormalities (cretinism), hemorrhage, delayedossification on metacarpus and metatarsus, and costae malformation of the foetus.
Kualitas Spermatozoa Kauda Epididimis Sapi Bali dengan Penambahan Laktosa atau Maltosa yang Dipreservasi pada Suhu 3–5oC Jusak Labetubun; Isak Piter Siwa
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The purpose of this research was to examine the effectivity of lactose or maltose in maintaining theviability of cauda epididymal spermatozoa at Bali cattle when preserved at 3-5 oC. Epididymal spermatozoawere collected by slicing, flashing and pressure of cauda epididymis with normal saline ( 0,9% NaCl). Thecollected spermatozoa were divided in equal volume into three different tubes where each tube was thendiluted with : i) 20 % egg yolk Tris solution (control), ii) 20 % egg yolk Tris solution + 0.6 gram lactose/ 100ml (L 0.6), iii) 20 % egg yolk Tris solution + 0.6 gram maltose/ 100 ml (M 0.6), respectively following this alltubes were kept in a refrigerator at 3-5 oC for six days. The quality of spermatozoa including percentage ofmotility, live, and intact plasma membrane (IPM) were evaluated daily for the six days period. Result atthe study showed that the mean spermatozoa concentration was 11.2 million cells/ml, whilst the mean atpercentage of motility, percentage of live, percentage of abnormality, percentage of cytoplasmic dropletsand percentage of IPM were 75.00%, 86.75%, 10.50%, 14.00% and 86.75%, respectively. At day 7 ofstorage, the percentage of motility, live, and IPM when the spermatozoa were preserved in media containinglactose was 39.00%, 51.40%, and 51.80%, respectively whilst when preserved in media containing maltosewas 38.00%, 49.80% and 52.00%, respectively. The percentage of motility, live and IPM was significantlyhigher (p<0.05) compared to the control. It is therefore concluded that the addition of lactose or maltose inthe preservation media could maintain the quality of the epididymal spermatozoa of Bali cattle.
Prevalensi dan Faktor Risiko Penyakit Virus Parvo pada Anjing di Denpasar I Nyoman Suartha; Diana Mustikawati; I Gusti Made Krisna Erawan; Sri Kayati Widyastuti
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The prevalence and risk factors of canine parvovirus disease in Denpasar were determined using datacollected from private veterinary practioners between 2004-2007. The risk factors observed including :dogs breed, age, sex, vaccination status, season, humidity, and temperature. Diagnosis of parvovirusinfection was done based on clinical signs, confirmed laboratory test and/or rapid test using parvoviruskit. The prevalence of canine parvovirus infection was 3.26% during 2004-2007. It appeared that incompletevaccination would increased the risk of the infection by 10.15 fold (OR 10.15). In addition the risk ofparvovirus infection would increased by 3.09 fold (OR 3.09) when dogs in 0-3 months old in comparison totheese age > 3months. There were not difference in susceptibelity to the infection between male andfemale dogs. Similarly, the occurence of canine parvovirus infection is not significantly affected by season,temperature, and humidity. Whilst the dog breed such as the Rottweiler, Pomeranian, Minipincher, andChihuahua were susceptible to the infection compared to other breed.

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