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I G. Made Krisna Erawan
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Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia
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INDONESIA
Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
Respons Antibodi Sekunder Terhadap Penyakit Tetelo pada Ayam Petelur Pascavaksinasi Ulangan dengan Vaksin Tetelo Aktif (NEWCASTLE DISEASESECONDARY ANTIBODY RESPONSE AFTER REVACCINATION IN LAYER WITH THE ACTIVE ND VACCINE) Andika Budi Kurnianto; Gusti Ayu Yuniati Kencana; I Nyoman Mantik Astawa
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Revaccination is required in order to preventNewcastle Disease (ND) reccurence inlayers chickens. Oneof vaccine for ND revaccination is freeze-died ND active vaccine containing e” 106,5EID50. Revaccinationisdone to trigger a faster secondary antibody responses in layers and can achieve protective antibody titersagainst ND that can be monitored by a hemagglutinationinhibition (HI). The aim of this study was todetermine the ND secondary antibody responses in layers after revaccination with ND active vaccine.Antibody titer of 20 layers chickens of 20 week old were determined before revaccinations (week 0) andafter revaccinations (week 1 until week 9). The first vaccination was conducted using ND-IB (NewcastleDisease-Infectious Bronchitis) at the age of 2 days through eye drops and subcutaneous injection at the ageof 5 days using a dose of 1 ampoule.Vaccination is repeated at the age of 20 weeks at a dose of 1 ½ ampoule through drinking water. Blood samples were collected on the wing vein (venous cutane ulnar) and left for 5-10 minutes at room temperature.Sera were then collected and stored at -20oC until use. HI antibody titerwas determined by micro titeration system. The HI mean titers were analyzed by Duncan test. The studyresults showed that antibody titer before revaccination was3,47 HI log 2 and the HI titers after revaccinationwere 4,02; 5,22; 6,52; 7,85; 8,4; 8,6; 7,7; 5,92; dan 3,87 HI log 2 respectivelly at weeks 1, 2, 3, 4, 5, 6, 7, 8, and9.The NDV revaccination with ND active vaccine significantly (P <0.01) increased in antibody titer inlayers starting from week 1 to week 6, but decreased following week 7 to week-9. It can be concluded thatrevaccinantion with ND active vaccine increases the antibody titers in layer chickens.
Resistansi Escherichia coli terhadap Kolistin dan Deteksi Gen Mobilized Colistin Resistance-1 pada Ayam Pedaging Akibat Pemberian Kolistin Sulfat Maria Fatima Palupi; Hera Maheshwari; Huda Salahuddin Darusman; Etih Sudarnika; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.653 KB) | DOI: 10.19087/jveteriner.2018.19.2.196

Abstract

Colistin sulphate is the ultimate antimicrobial choice for the treatment of multidrug resistance gram negative bacteria infections with in human. The purposes of this study were to detect the presence of colistin resistant E. coli and mcr-1 gene in broiler and to transfer the mcr-1 gene to Salmonella enteritidis ATCC 13076. A total of 54 one day old broilers were divided into three groups that consists of 18 chicks broiler per group and raised up to 40 days old. The first group was used as control. The first treatment group was given colistin sulphate 5 ìg/g feed for 40 days and broilers in second treatment group was given 80.000 IU/kg body weight for first three days. Swab cloaca samples were taken every 10 days from each broiler. At age 40 days all chickens were slaughtered and meat samples were collected. Samples of cloacal swabs, fresh and cooked meat were examined for the presence of colistin resistant E. coli and mcr-1 gene. Susceptibility to colistin sulfate was conducted by agar dilution method, and detection of mcr-1 gene was conducted using polymerase chain reaction. The results showed that no colistin resistant E. coli was detected in the control group. Colistin resistant E. coli (27.78%) and mcr-1 gene (20.00%) were detected in animals in the first treatment group, respectively. Whilst 11.11% colistin resistant E. coli and 5.56% were carriying mcr-1 gene in the second treatment group. Colistin resistant E. coli were found 5.56% from raw meat samples and 3.70% had mcr-1 gene. Transfer of mcr-1 gene from colistin resistant E. coli to Salmonella enteritidis ATCC 13076 was success. These results showed the necessity of limitation usage of colistin sulphate in food animal.
Daya Larvasida Ekstrak Daun Muda Kedondong Hutan Terhadap Haemonchus contortus Secara In-vitro (LARVICIDAL EFFECT OF SPONDIAS PINNATA LEAF EXTRACT AGAINST HAEMONCHUS CONTORTUS IN VITRO) I Gusti Komang Oka Wirawan; Wisnu Nurcahyo; Joko Prastowo; Kurniasih Kurniasih
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.02 KB) | DOI: 10.19087/jveteriner.2017.18.2.283

Abstract

This study aimed to asses effective concentration of condensed tannins in the extract of Lannea grandis leaves (EDMKH) as larvacide anti-helminths on Haemonchus contortus. Experimental design used in this study was completely randomised design. The treatments group were administrated with 2.5%, 3.5% , and 4.5% EDMKH in the 0.2 g/mL solution, respectively. Moreover aqueduct was used for the negative control group, while 0.055% albendazole was applied to the positive control group. Variables measured were percentage of developed and undeveloped L1 in the incubation media. The data were the statistically analysed following General Linear Model format for completely randomised design. The software used in the analysis was SPSS v. 23.0 (IBM-SPSS 2015). Results showed that among the EDMKH treatments, the lowest larvacidal effect was recorded for 2.5% EDMKH treatment compared with 3.5% and 4.5% EDMKH. However, the value achieved by 2.5% EDMKH treatment was significantly higher (P<0.05) than that of negative control treatment. The highest larvacidal effect (100%) was shown by 4.5% EDMKH treatment which was not significantly different (P>0.05) from the positive control treatment. ABSTRAK Penelitian ini bertujuan untuk mengetahui konsentrasi efektif tanin kondensasi di dalam Ekstrak Daun Muda Kedondong Hutan (EDMKH) sebagai antelmintik yang bersifat larvasida terhadap Haemonchus contortus (H. contortus) secara in-vitro. Rancangan penelitian yang digunakan dalam penelitiam ini adalah Rancangan Acak Lengkap (RAL), dengan lima perlakuan yaitu tiga perlakuan EDMKH konsentrasi: 2,5%, 3,5%, 4,5% dari 0,2 g/mL larutan tersedia, satu kelompok kontrol (-) menggunakan air suling, satu kelompok kontrol (+) menggunakan albendazole konsentrasi 0,055%. Variabel yang diukur dan dianalisis: jumlah persentase L1 yang berkembang dan tidak berkembang dalam kelompok rendaman. Data persentase daya larvasida H. contortus dari konsentrasi tanin kondensasi EDMKH secara in-vitro dianalisis menggunakan analisis varian mengikuti prosedur Rancangan Acak Lengkap (RAL) menggunakan SPSS Versi 23(IBM-SPSS 2015). Hasil analisis statistika menunjukkan bahwa persentase larvasida EDMKH 2,5% nyata lebih rendah dibandingkan 3,5%, 4,5% dan albendazole 0,055% (P<0,05) tetapi lebih tinggi dari kontrol negatif. Konsentrasi efektif EDMKH terhadap larva H. contortus adalah konsentrasi 4,5%, memberikan efektivitas larvasida 100%, dan tidak berbeda nyata dengan kontrol positif (P>0,05).
Umur Memengaruhi Volume Semen dan Motilitas Spermatozoa Babi Landrace di Balai Inseminasi Buatan Baturiti, Tabanan, Bali (AGE AFFECTS SEMEN VOLUME AND MOTILITY OF SPERMATOZOA LANDRACE BOAR’S OF BATURITI ARTIFICIAL INSEMINATION CENTER, TABANAN, BALI) Ni Luh Gde Sumardani; Komang Budaarsa; Tjok Istri Putri; Antonius Wayan Puger
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.302 KB) | DOI: 10.19087/jveteriner.2019.20.3.324

Abstract

Sperm quality have been associated with age for many years. This study aims to determine the inffluence of age to the volume and motility of Landrace boar’s sperm at Baturiti Artificial Insemination (AI) center. A Total of 300 ejaculates were used in this study. An ejaculate origin from five boar are a collection of five month. A complete randomized design (CRD) was used with two different boar of block ages, block A (2-4 year) and block B (6-8 year). Three or four glass slides were prepared for each boar sample; a drop of semen was placed on each glass slides. This sample was examined under the light microscope on five view field in each glass slides. The result of the research show that the Landrace boars in block A have semen volume average 273.60 mL and sperm motility 73.86%, while in block B the average semen volume was 107.66 mL and sperm motility 62.92%. It can be concluded that Landrace boars in block A had higher volume and sperm motility compared to Landrace boars in block B.
Penurunan Kadar Gula Darah pada Monyet Ekor Panjang Obes dengan Pemberian Nikotin Dosis Rendah (DECRESE IN BLOOD SUGAR LEVEL IN LONG TAILED OBESE MACAQUES GIVING WITH LOW DOSE NICOTINE) Chusnul Choliq; Dondin Sajuthi; Irma Herawati Suparto; Dewi Apri Astuti; Retno Wulansari
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the study was to evaluate the role of low dose nicotine on the profile of blood glucose and  â-cell of pancreatic islets.  Fourteen adult (aged 6 – 8 years) male cynomolgus monkeys grouped based ontheir Body Mass Index (BMI) into preobese (BMI=23.65 – 25.00) and obese (BMI e” 26.00) were used inthis study. Subsequently animals were grouped into four:  (i) preobese monkeys with nicotine (pOb+), (ii)obese monkeys with nicotine (Ob+), (iii) preobese monkeys without nicotine (pOb-), and (iv) obese monkeyswithout nicotine (Ob-). Animals in the nicotine groups were fed with high fat diet mixed with nicotine dose0.5–0.75mg/kg body weight/day for three months and the others were fed Monkey Chow® only as thecontrol group. Blood samples were collected every month for glucose analysis and necropsy was performedat the end of study. Pancreas tissues were processed histologically and stained using  immunohistochemicalmethod. The results showed that the blood glucose either preobese (28.37%) or  obese (33.72%) animals inthe nicotine groups significantly decrease (p<0.05) during the study period in comparison to the controlgroup.  Based on brown color intensity of granules cytoplasm of insulin producing cells or immunoreactiveâ-cells, it is shown that the cells of animals in the non nicotine group were more reactive than those in thenicotine groups. In conclusion, there was positive effect of low dose nicotine in maintaining the bloodglucose level in normal range by stimulation of islet cells proliferation to maintain the production ofinsulin in the pancreatic islet.
Kandungan Mikrob Daging Kelelawar yang Diolah sebagai Bahan Pangan Tradisional MICROBIAL CONTENT IN THE PROCESSED MEAT BATS AS TRADITIONAL FOOD Tiltje Andretha Ransaleleh; Rarah Ratih Adjie Maheswari; Purwantiningsih Sugita; Wasmen Manalu
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was designed to assess the microbial characteristics of bats meat which were cooked askari and rica-rica, and were stored up to 14 days.The study was divided into two stages, the first stage usedthree types of bat meat that were cooked as rica-rica and kari 14 days after being stored in the refrigerator.The second stage used meat of a species of bat that was cooked as rica-rica and kari a few hours afterslaughtering. The result of the meat analysis at the 1st showd that thetotal count of microbials i.e;Staphylococcus aureus, Escherichia coli, coliform, and Salmonella sp from three species of bats that werecooked rica-rica  and  kari  were above the maximum limit of microbial contaminant occording to BSN(2009). The analysed made on the rica-rica showed that bat meat rica-rica stored up to 14 days containedtotal microbial count of 3,1 x 104 - 6,0 x 104Cfu/mL, Staphylococcus aureus 7,7 x 101- 7,6 x 103 Cfu / mL,Escherichia coli and Salmonella sp was negative. The total microbial count  of bat meat kari was  6,8 x 105-9,7 x 105Cfu/mL,  Staphylococcus aureus  was 4,3 x 101-1 x 104 Cfu /mL.  Escherichia  coli <3 / mL, andSalmonella sp was negative. Based on the result abtained, it was concluded that the bat meat rica-ricaand kari which were cooked in a few hours after cutting and stored until 14 days were suitable for humanconsumption.
Pelacakan Secara Imunohistokimiawi Antigen Ekskretori-Sekretori pada Sapi Bali yang Terinfeksi Fasciola gigantica (IMMUNOHISTOCHEMICAL DETECTION OF EXCRET0RY-SECRETORY ANTIGENS IN BALI CATLLE INFECTED BY FASCIOLA GIGANTICA) Ida Bagus Oka Winaya; I Nyoman Mantik Astawa; I Made Damriyasa; Nyoman Sadra Dharmawan; I Ketut Berata
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In order to study the distribution of excretory-secretory (ES) F. gigantica in liver tissue of infected balicattle a research was establisihed using monoclonal antibodies (mAbs) againts ES antigens. Immortalmouse myeloma cells were fused with the lymphocytes derived from the spleen of mice that immunizedwith ES antigen. The mAbs were tested for their specificity by using enzyme linked immunosorbent assay(ELISA). Five specific mAbs againts ES antigens were isolated and two mAbs were used for immunodetectionof ES antigens in liver tissue of bali cattle. Immunohistochemical ES antigens were not detected in paraffinembeded tissue of negative confirmed fasciolosis samples. ES antigens was detected in hepatocytes andcytoplasm of bile duct epithelims in the bali cattle that infected with fasciolosis in moderate intensity.Therfore indicated that mAbs produced in this study are applicable for detecting ES antigens in bali cattleinfected by F. gigantica.
Antigen Ekskretori-Sekretori Cacing Jantung (Dirofilaria immitis) Jantan dan Betina yang Berpotensi Sebagai Marka Diagnosis (EXCRETORY-SECRETORY ANTIGENS OF MALE AND FEMALE HEART WORMS (DIROFILARIA IMMITIS) WHICH POTENTIALLY AS A DIAGNOSTIC MARKER) I Gusti Made Krisna Erawan; Ida Tjahajati; Wisnu Nurcahyo; Widya Asmara
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Heart worm (Dirofilaria immitis) is the causative agent of a serious parasitic disease in dogs.Dirofilariasis is generally diagnosed by microfilariae examination and specific antigen testing. Microfilariaeexamination has low sensitivity due to occult infections. The available antigen test at this time is able todetect circulating antigens secreted by adult female worms only. The aim of the present study was toidentify male (MES) and female (FES) heart worms excretory-secretory antigens which have the potentialas a diagnostic targets. Identification of antigen was done by sodium dodecyl sulphate polyacrylamide gelelectrophoresis (SDS-PAGE) and Western Blotting analysis. The results of this study indicated that therewere differences between the MES and the FES profiles. The results showed 12 bands in MES (14–118kDa) and 18 bands in FES (10–205 kDa). Protein with a molecular weight of 59 kDa has the potential asdiagnostic markers of dirofilariasis.
Kadar Glukosa dan Trigliserida Darah Kerbau Karapan yang Diberi Latihan dengan Interval Berbeda (BLOOD GLUCOSE AND TRIGLYSERIDE LEVELS OF EXERCISING SWAMP BUFFALOES UNDER VARIOUS EXERCISE INTERVAL) Nyoman Sadra Dharmawan; I Gede Mahardika
Jurnal Veteriner Vol 2 No 3 (2001)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Abstak dapat dibaca pada Full Text Abstract can be read at Full Text
Identifikasi Gangguan Reproduksi Sapi Betina di Peternakan Rakyat (IDENTIFICATION OF REPRODUCTIVE DISORDERS IN FEMALE CATTLE AT LOCAL FARMS) Sutiyono Sutiyono; Daud Samsudewa; Alam Suryawijaya
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.276 KB) | DOI: 10.19087/jveteriner.2017.18.4.580

Abstract

The aim of this research was to determine the female reproductive disorders in cattle reared by local farmers in the Distric of Kaliori, Rembang Regency, Central Java Province. A total of 94 cattle were used, in which had minimal one of each incisors had been replaced. The study used survey methods, and data were collected by interviewing with ranchers, rectal palpation, identification of the incisors, and body condition score of the cattle. In the implementation of the study, cattle were taken to a place determined by the chairman of the group of farmers (field or home page). The parameters of study were unheard of oestrus or not, the amount of artificial insemination, the number of incisors changed, body condition score of each cattle, the feed given, and their maintenance. The data were analyzed using statistical descriptive analysis on the mode, range, and percentage. The results showed that of the 94 cattle, which have disorders of reproductive activity as much as 80. Samples with impaired reproductive activity were divided into three groups. The first group was the old heifers that had no oestrus 25.00%, the second group was cattle that were more than three times applied artificial insemination and had not been pregnant 45.00%, and the third group was cattle that more than three months after the last giving birth had no oestrus 30.00%. The other reproductive disorders that occured in individual of the cattle was inactive ovaries (follicle undeveloped) 2.50%, 6.25% ovary hypofunction, ovarian cystic 1.25%, endometritis 2.50% and 2.50% abnormal uterus. In conclusion, the largest reproductive disorders in cattle caused by nutritional factors that provided by the farmers, and small disturbances due to some diseases and abnormal reproductive organs.

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