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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
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Peningkatan Ekspresi Vascular Endothel Growth Factor dan Mitogen Activating Protein Kinase Plasenta Tikus yang Dipapar Carbon Black ((INCREASING EXPRESSION ON VASCULAR ENDOTHELIAL GROWTH FACTOR AND MITOGEN ACTIVATING PROTEIN KINASE IN PLACENTA RATS EXPOSE Viski Fitri Hendrawan; Widjiati Widjiati; Suherni Susilowati; Pudji Srianto
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.745 KB) | DOI: 10.19087/jveteriner.2017.18.1.59

Abstract

Carbon black is one of the components of particulate matter (PM), known as cytotoxic, causing inflammationin the respiratory and cardiovascular system, and able to pass through the placental barrier, which may affect the fetus. The effects caused by pollutants depend on the intensity and time length of the exposure.The objective of this study is to identify Vascular Endothelial Growth Factor (VEGF) and Mitogen Activating Protein Kinase (MAPK) as an indicator of maternal placental inflammation in pregnant rats (Rattus norvegicus) exposed to carbon black with immunohistochemical technique. This study was a laboratory experiment with three treatment groups and seven replications using pregnant female white rats (Rattus norvegicus). Pregnant female rats were exposed to carbon black with a dosage of 523 mg/m3 and 1064 mg/m3 during pregnancy of day 6 up to day 11. The next phase was the observation and examination of the expressions of VEGF and MAPK by immunohistochemical staining toward placenta of the white rats exposed to carbon black. The result of the study showed an increase in the expression of VEGF in the placenta of the rats exposed to carbon black with a dosage of 523 mg/m3 and 1064 mg/m3 per inhale during the mid-period (6-11) of pregnancy. In addition, there was also an increasein the expression of MAPK on the placenta of the rats exposed to carbon black with a dosage of 523 mg/m3and 1064 mg/m3 per inhale during the mid-period (day 6-11) of pregnancy. In conclusion, the expression of VEGF and MAPK are increased along with the increased dosage of carbon black and age of gestation. ABSTRAK Carbon black merupakan salah satu komponen partikulat matter (PM) yang diketahui bersifat sitotoksik serta menyebabkan terjadinya inflamasi pada sistem pernapasan dan kardiovaskular serta dapat melewati barier plasenta fetus sehingga dapat memengaruhi fetus. Efek yang ditimbulkan oleh polutan tergantung dari besarnya paparan dan lama waktu paparan. Penelitian ini bertujuan untuk mengidentifikasi Vascular Endothel Growth Factor (VEGF) dan Mitogen Activating Protein Kinase (MAPK) sebagai indikator imflamasi pada plasenta maternal tikus (Rattus norvegicus) yang dipapar carbon black dengan teknik imunohistokimia. Penelitian ini dilakukan dengan tiga kelompok perlakuan dan tujuh kali ulangan yang menggunakan tikus betina bunting. Tikus betina bunting dipapar dengan carbon black dosis 523 mg/m3 dan 1064 mg/m3 selama kebuntingan hari ke-6 sampai 11. Tahap berikutnya adalah pengamatan dan pemeriksaan ekspresi VEGF dan MAPK dengan pewarnaan imunohistokimia terhadap plasenta tikus putih yang telah dipapar dengan carbon black. Hasil penelitian adalah terjadi peningkatan ekspresi VEGF pada plasenta tikus yang dipapar carbon black dosis 523 mg/m3 dan 1064 mg/m3 per inhalasi selama masa pertengahan (6-11) kebuntingan. Selain itu terjadi peningkatan ekspresi MAPK pada plasenta tikus yang dipapar carbon black dosis 523 mg/m3 dan 1064 mg/m3 per inhalasi selama masa pertengahan(6-11) kebuntingan. Simpulan penelitian ini adalah ekspresi VEGF dan MAPK meningkat seiring dengan peningkatan dosis carbon black dan umur kebuntingan.
Kualitas Sperma Beku Sapi Bali dalam Pengencer Air Kelapa Modifikasi dengan Berbagai Aras Dimethyl Sulfoxide (FROZEN SPERM QUALITY OF BALI BULLS IN MODIFIED COCONUT WATER EXTENDER WITH DIFFERENT DIMETHYL SULFOXIDE CONCENTRATION) Thomas Mata Hine; Kirenius Uly; Wilmientje Marlene Nalley; Heri Armadianto
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (106.838 KB) | DOI: 10.19087/jveteriner.2019.20.1.93

Abstract

Dimethyl Sulfoxide (DMSO) is one type of cryoprotectant which has a low molecular weight so that it is easier to enter cells when cryopreservation. The purpose of this study was to explore the optimal concentration of DMSO in modified coconut water (mCW) extender that were able to maintain frozen sperm quality of bali bulls. Semen was collected from two four-year old bali bulls by artificial vagina. Good quality semen diluted with mCW (young coconut water + 20% egg yolk + 7.5 % moringa leaf extract) and supplemented by 3, 5, or 7% DMSO. Semen was filled into 0.25 ml ministraw, and was incubated in a refrigerator at 5°C for four hours, frozen on the surface of liquid nitrogen for 10 minutes and then dipped into liquid nitrogen. The quality of post thawing sperm was measured 24 hours later by placing the ministraw of frozen semen into water at 37oC for 30 seconds. Data were analyzed by analysis of variance and continued with Duncan test. Postthawing observations showed that bali bulls sperm cryopreserved at 3% DMSO yielded higher motility and viability (p<0.05) i.e. 36 and 44.15%, than DMSO 5% i.e. 18 and 23.65%, and DMSO 7% i.e. 7 and 12.62%. The recovery rate of sperm cryopreserved at 3% DMSO was also higher (p<0.05) than DMSO 5 and 7%, successively 45.65, 23.06, and 8.86%. The results of this study concluded that the optimal concentration of DMSO in mCW diluent to maintain frozen sperm quality of bali bulls was 3%.
Kadar Prostaglandin F2? pada Cairan Vesikula Seminalis dan Produk Sel Monolayer Vesikula Seminalis Sapi Bali (CONCENTRATIONS OF PROSTAGLANDIN F2? IN SEMINAL VESICLE FLUID AND PRODUCT OF SEMINAL VESICLE MONOLAYER CELLS OF BALI CATTLE) Tjok Gde Oka Pemayun
Jurnal Veteriner Vol 8 No 4 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (213.056 KB)

Abstract

In this study, the concentration of prostaglandin F2 ? (PGF2?) in seminal vesicle fluid and seminal vesicle monolayer cell cultures of Bali cattle was determined. The seminal vesicle fluid was aspirated and the epithelial cells of the seminal vesicles were cultured in tissue culture medium (TCM) 199 growth medium containing 10% fetal calf serum (FCS) and 10% oestrus mares serum (EMS) with a density of 1.9 x 106 cells / ml medium. Following an incubation at 38.50 C in 5% CO2 atmosphere for 6 days and the level of PGF2 ? in the original seminal vesicle fluid and in the cell culture medium were determined by radioimmunoassay techniques (RIA). The results showed that the level of PGF2 ? in the non-extracted monolayer culture of seminal vesicle (1287,50 ± 3,39 pg/ml ) was significantly higher than that of detected in non-extracted seminal vesicle fluid (1,23 ± 0,79 pg/ml). In contrast, after extraction the level of PGF2 ? in seminal vesicle monolayer cell cultures (218,33 ± 2,87 pg/ml) significantly decreased as compared to seminal vesicle fluid (1750,83 ± 2,71 pg/ml). In conclusion the highest level of PGF2 ? was found in the extract of seminal vesicle fluid.
PERUBAHAN VIABILITAS DAN STRUKTUR SUBSELULER SPERMATOZOA DOMBA SETELAH PENGERINGBEKUAN Takdir Saili; I Ketut Mudite Adnyana; Ronny Rachman Noor; Mohamad Agus Setiadi; Srihadi Agungpriyono; Arief Boediono
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Several methods i.e. cooling, freezing, and freeze-drying have been widely used to preserve spermatozoa with various degree of success. Freeze-drying appears to provide a method to preserve spermatozoa in a dry state without requiring liquid nitrogen for storing frozen spermatozoa. Freeze-drying procedures can have a detrimental effect on plasma membrane and acrosomal cap of the spermatozoa. In this experiment study, the viability and subcellular changes of freeze-dried ram spermatozoa were evaluated using staining method and scanning electron microscopy. The results revealed that all freeze-dried spermatozoa were dead following evaluation using eosin staining and Hoechst-propidium iodide staining methods. Morover, plasma membrane and acrosomal cap of freeze-dried ram spermatozoa was disrupted observed using scanning electron microscope.
Identifikasi Clinostomum complanatum Secara Molekuler pada Ikan Air Tawar di Yogyakarta dan Riau (IDENTIFICATION OF Clinostomum Complanatum FROM FRESHWATER FISH IN YOGYAKARTA AND RIAU BASED ON MOLECULAR STUDY) Morina Riauwaty; Kurniasih .; Joko Prastowo; Windarti .
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of study was, to identify Clinostomum complanatum (Digenea: Clinostomidae) infectingfreshwater fish in Yogyakarta and Riau on the bases of their molecular profiles in the internal transcribedspacer region (ITS1). Samples of climbing gouramy (Anabas testudineus) infected by Clinostomum sp. wereobtained from Kali Progo River, Yogyakarta. Whereas the climbing perch (Trichogaster trichopterus) wereobtained from the Sail River, Riau. Metacercariae of Clinostomum sp. found in the gills and visceralorgans were aseptically removed using needle, preserved in absolute ethanol. Molecular examination wasperformed by Polymerase Chain Reaction method consisted of extraction, amplification, electrophoresisand sequencing of DNA sample. The DNA sewuences of the samples were analysed by maximum parsimonyand neighbour-joining method. Phylogenetic analysis showed that Clinostomum sp. from Yogyakarta wasgenetically idential to Clinostomum complanatum, whereas Clinostomum sp. from Riau was geneticallysuspected as a new species (difference > 2%) which is included in one cluster to Clinostomum phalacrocorasis.
Identifikasi Trenggiling (Manis javanica) Menggunakan Penanda Cytochrome B Mitokondria DNA (IDENTIFICATION OF PANGOLIN (MANIS JAVANICA DESMAREST, 1822) USING CYTOCHROME B mtDNA MARKER) Wirdateti .; Gono Semiadi; Yulianto .
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.636 KB)

Abstract

The aim of this study was to identify the gentic profile of Malay pangolin (Manis javanica) and originpatterns of confiscated specimens.  Tissue samples of Malay pangolin were collected from several confiscatedmaterials in Tangerang, Medan, and Lampung. Wild collections tissue were also conducted in Lampungand Sukabumi. The study was conducted using  conserved Cytochrome b (Cyt. B) DNA mitochondria(mtDNA). The results showed that based on nucleotide base lentgh of 420 nt, confiscated pangolin wasdistributed in three clades and two groups. Haplotype variations was high, consisted of 19 haplotypes in19 individuals (TR1-TR19). On fisrt clade (TR4,7,16,9,19) high substitution occured in adenin base, cladetwo (TR14,17,1,2,15,3,8,13) high substitution occured in guanin base and clade three  (TR5,6,10,11,12) incytosin. It was concluded that haplotipe variation of each populations  was high and for genetic distancebetween individuals was low. Mutation rates was dominated by transition  from guanine to adenine
Malassezia spp. dan Peranannya sebagai Penyebab Dermatitis pada Hewan Peliharaan (MALASSEZIA SPP AND ITS ROLE AS THE CAUSAL AGENT OF DERMATITIS IN PET ANIMALS) Pradipta Nuri Adiyati; Eko Sugeng Pribadi
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (256.783 KB)

Abstract

Malassezia is dimorphic yeast found normally in the animal healthy skin. Malassezia can causehealth problem in pet animals, such as dogs, cats, and other domestic animals. Varies according itsvirulence, Malassezia can cause skin changes characterized by severe pruritus, yellowish erythema andscab, greasy skin, bad odor with hyperpigmentation and lichenification in the face, paws, and neck bottom,as well as belly. Laboratory diagnosis can be performed either by microscopic examination of nativepreparations, or molecular biology. Treatment of Malassezia’s infection can still be made using someantifungals currently available. Malassezia pachydermatitis infection has been known as zoonoticpotential.
Ekstrak Etanol dan Fraksi Heksan Buah Pare (Momordica charantia) Sebagai Penurun Kadar Glukosa Darah Tikus Diabetes (ETHANOL EXTRACT AND HEXANE FRACTION OF MOMORDICA CHARANTIA DECREASE BLOOD GLUCOSE LEVEL OF DIABETIC RAT) I Nyoman Suartha; I Made Dira Swantara; Wiwik Susanah Rita
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The research on the potency of Momordica charantia as lowering blood glucose has been carried out.The fruit was extracted by 70 % ethanol at room temperature. The extract was then partition with NHexane.The filtrate of partition was purified with thin layer chromatography. Three months old of whitemale rats (Rattus novergicus) with approximately 200-250 grams in weight were used as Bio-indicators.This study used a randomized block design (CRD) consisting of eight treatment groups (each treatmentconsisted of five rats). Rats were injected with streptozotocin to get hyperglycemic condition. The M.charantia fruit fraction (fraction 1-5) with dose 100 mg/kg bw was treated to each group when the ratt wason hyperglycemic condition. Rat blood glucose levels were observed on days 0, 4, 11, and 18 respectively .The results showed that blood glucose levels of M. charantia fraction 1and 5 with dose of 100 mg/kg bwhave the same effect with a negative control from day 4th, fraction 2 on day 18 whereas The others fractionare 3, and 4 were effect on days 18th. Based on the result it can be concluded that the M. charantia fraction1 with dose of 100 mg/kg bw effectively in decreasing the blood glucose levels of diabetic rat.
Penentuan Protein Antigen Limfosit Sapi yang Berasal dari Sapi Bali dan Sapi Peranakan Ongole (DETERMINATION OF THE PROTEIN OF BOVINE LYMPHOCYTE ANTIGEN, OF BALI CATTLE AND ONGOLE CROSS BREED CATTLE) Ni Ketut Suwiti; Fedik Abdul Rantam
Jurnal Veteriner Vol 4 No 2 (2003)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Abstak dapat dibaca pada Full Text Abstract can be read at Full Text
Yuniati Kencana TOTAL DAN DIFERENSIAL LEUKOSIT AYAM PETELUR PASCAVAKSINASI NEWCASTLE DISEASE DAN AVIAN INFLUENZA Gusti Ayu Yuniati Kencana; Anak Agung Sagung Kendran; Luh Dewi Anggreni; Ni Wayan Helpina Widyasanti
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.586 KB) | DOI: 10.19087/jveteriner.2018.19.2.190

Abstract

The purpose of this study was to determine total and differential leukocyte of layer after vaccinated with Newcastle Disease (ND) and Avian Influenza (AI) combination vaccine. The research was conducted on commercial layer breeding in Denbatas Village, Tabanan Sub-district, Bali. This study used 30 layer samples. Vaccination was done with ND-AI combination vaccine which was a combination of ND vaccine strain Lasota with AI vaccine subtype H5N1. ND-AI combination vaccine was an inactive vaccine. Chicken was vaccinated at 18 weeks of age before laying eggs. Vaccination was done with one dose (0.5 ml) through thigh muscle injection. Chicken blood sampling was done twice ie, two weeks and three weeks after vaccination. The examination of the total leukocytes was performed by count chamber, whereas the differential leukocyte was examined from a blood smear preparation by Giemsa staining. Data of total and differential leukocyte examination were analyzed by descriptively quantitative. The results concluded that layer has leukopenia and heteropenia which were probably caused by hormonal factors and stress due to blood collection.

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