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I G. Made Krisna Erawan
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krisnaerawan@unud.ac.id
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Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia
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Kota denpasar,
Bali
INDONESIA
Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
Seroprevalensi dan Faktor Risiko Penularan Mycoplasma gallisepticum pada Peternakan Ayam Petelur Komersial di Kabupaten Blitar (SEROPREVALENCE AND( RISK FACTORS OF MYCOPLASMA GALLISEPTICUM INFECTION IN COMMERCIAL LAYER FARM IN BLITAR DISTRICT) Diyantoro Diyantoro; I Wayan Teguh Wibawan; Eko Sugeng Pribadi
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.237 KB) | DOI: 10.19087/jveteriner.2017.18.2.211

Abstract

This study aimed to figure out the prevalence and risk factors of Mycoplasma gallisepticum (MG) infection in commercial layer farm in Blitar District. Blood samples and questionnaires were taken during December 2014 to February 2015. A total of 264 sera samples were collected from 22 commercial layer farm. Based on serum plate agglutination test, 26 sera samples were MG positive that were indicated an infection prevalence was 9.85%. The highest SRR value for MG infection was occurred in Bakung Subdistrict (SRR = 2.5). Based on Analysis of multivariate logistic regression showed that a very significant influenced risk factors of MG infection have occurred in flocking density more than 3,000 birds per flock (?2= 11.10; p= 0.001; OR= 6.1), flocking density about 1,501 to 3,000 birds per flock (?2 = 11.10; p= 0.001; OR= 6.1), bird feeding once a day (?2= 9.32; p= 0.002; OR= 0.3), house desinfection once in every two weeks (?2 =7.70; p= 0.009; OR= 1.2), house desinfection once a month or only in case (?2= 9.36; p= 0.006; OR= 3.9). It was concluded that seroprevalence of MG infection in studied area was 9,85%. the MG seroprevalence were influenced by flocking density more than 3,000 birds per flock, flocking density about 1,501 to 3,000 birds per flock, bird feeding once a day, house desinfection once every two weeks, and house desinfection once a month or only in case. ABSTRAK Mycoplasmosis merupakan salah satu penyakit paling penting yang dihadapi oleh industri perunggasan di Indonesia. Penelitian ini bertujuan untuk mengetahui prevalensi dan faktor risiko penularan Mycoplasma gallisepticum (MG) pada peternakan ayam petelur komersial di Kabupaten Blitar, Jawa Timur. Contoh darah dan kuisioner diambil selama periode Desember 2014 hingga Februari 2015. Sebanyak 264 contoh serum dikumpulkan dari 22 peternakan ayam petelur komersial. Berdasarkan uji Rapid Serum Agglutination (RSA), 26 contoh ditemukan positif MG dengan prevalensi infeksi MG di Kabupaten Blitar sebesar 9,85%. Nilai rasio risiko terbakukan (standardized risk ratio, SRR) untuk penularan MG paling tinggi adalah Kecamatan Bakung (SRR = 2,5). Hasil analisis regresi logistik multivariate didapatkan bahwa faktor yang berpengaruh sangat nyata (P<0,01) terhadap terjadinya infeksi MG adalah kepadatan flok di atas 3.000 ekor ( ?2= 11,10; p= 0,001; OR= 6,1), kepadatan flok 500-1.500 ekor (?2 = 11,09; p= 0,004; OR= 3,4), pemberian pakan satu kali dalam sehari (?2= 9,32; p= 0,002; OR=0,3), penyemprotan kandang yang dilakukan satu kali dalam dua minggu (?2 = 7,70; p= 0,009; OR= 1,2),dan penyemprotan kandang yang hanya dilakukan satu kali dalam sebulan atau hanya jika terjadi kasus ?2 ( = 9,36; p= 0,006; OR= 3,9). Hasil penelitian dapat disimpulkan bahwa prevalensi infeksi MG di daerah kajian adalah 9,85%. Faktor risiko yang berpengaruh sangat nyata terhadap penularan MG antara lain jumlah ayam yang dipelihara di atas 3.000 ekor per kandang, jumlah ayam yang dipelihara sebanyak 1.501- 3.000 ekor per kandang, pemberian pakan satu kali sehari, penyemprotan kandang satu kali dalam dua minggu, dan penyemprotan kandang satu kali sebulan atau hanya jika ada kasus. Mycoplamosis is one of an important poultry diseases that should be well managed by poultry farmer in Indonesia.
Protective Immune Response of Post Rabies Vaccinated Dogs in Buduk Village, Mengwi, Badung, Bali (RESPONS IMUN PROTEKTIF PADA ANJING PASCAVAKSINASI RABIES DI DESA BUDUK, MENGWI, BADUNG, BALI) Ida Bagus Kade Suardana; Ida Bagus Oka Winaya; Desak Wiga Puspita Dewi; I Wayan Sudarmayasa; Kadek Karang Agustina
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (94.654 KB) | DOI: 10.19087/jveteriner.2019.20.2.264

Abstract

In efforts to eradicate rabies disease, vaccinations have been carried out targeting so-called rabies carrier animal such as dog andcat. Mass rabies vaccination has been done by the government of Bali annualy, in every sub-village at vaccination post or at every household. Minimum vaccination coverage to protect the threatened dog population from rabies is 70%. To determine whether the vaccinated dogs have protective antibody against rabies, this study have been done to find out the immune response of dogs after rabies vaccination in Buduk village. Sera were collected using random sampling in each subvillage, a total of 30 serum sample were examined, and the antibody was tested using kit ELISA Rabies®. Elisa test resulted in 90% of dogs have protective antibody titers (OD>0.5 EU). It concluded that dogs in Buduk village have a good immune response against rabies vaccine.
Patogenesitas Virus Gumboro Isolat Lokal pada Ayam Pedaging Sutiastuti Wahyuwardani; Dewi Ratih Agungpriyono; Lies Parede; Wasmen Manalu
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (378.26 KB)

Abstract

Study of the pathogenicity of gumboro virus (very virulent Infectious Bursal Disease virus, vvIBDV) oflocal isolates was performed in broilers. The chickens were grouped into four: i) infected with vvIBDV; ii)vaccinated with commercial vaccine + challenge with vvIBDV; iii) vaccinated using locally produced vaccine+ challenged with vvIBDV; and iv) control group (unvaccinated animals). Pathogenecity was analyzedbased on the sequence of infection; the distribution and degree of gross pathology and histopathologylesions on bursa Fabricius, spleen and thymus in relation to the presence of the antigen (IBDV Ag) whichwas detected using immunohistochemistry technique. During the acute phase (1-3 days following theexperimental infection) hyperemia and exudation on the bursa Fabricius were observed in the infectedgroup and the two vaccinated-challenged groups. Similarly, hyperemia was also observed on the spleenand thymus. Whilst during the chronic stages (at 7 and 14 days following the experimental infection) thebursa showed atrophy. Upon histological examination, the bursa showed interstitial edema, hemorrhage,infiltration of heterophyl cells and fibroblast hyperplasia. The bursa lymphoid follicles were depleted dueto cells necrosis and apoptosis. Plica epithelial layer was wrinkled and the Goblet cells metaplasia formedhyper plastic cysts. In the spleen and thymus severe reticulo endothelial cells proliferation was observedduring the acute stage and tended to decline during the chronic stage. During the acute and chronic stagescells containing IBDV Ag were detected in the bursa Fabricius, distributed within the mantle zone andgerminal center of lymphoid follicles in the two vaccinated-challenge groups. Whereas in the infectedgroup, the IBDV antigen were detected up to 7 days post the infection. Nevertheless, neither IBDV antigenwas detected in the spleen nor in the thymus in all the experimental groups. The results of this studyclearly explained the ferocity of vvIBDV of local isolates.
Pengembangan Antibodi Poliklonal dari Stadium Oosista, Sporosista, dan Sporozoit Eimeria tenella (THE DEVELOPMENT OF POLYCLONAL ANTOBODY FROM EIMERIA TENELLA OOCYST, SPOROCYST, AND SPOROZOITE STADIUM) Galuh Tresnani; Joko Prastowo; Wisnu Nurcahyo; Budi Setiadi Daryono
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.074 KB)

Abstract

The research on developing diagnostic method, vaccine, and drugs for coccidiosis has been focused onthe finding of the immunogenic molecule in Eimeria. The identification of this agent will need the antibodywhich can recognize the biomolecule in the antigen. Antibody that has been developed for this purposeshould be analyzed first, and one of the simple methods for analyzing this antibody is through dot blotanalysis. The objective of this research was to analyze the polyclonal antibody which developed from theoocyst, sporocyst, and sporozoite of  E. tenella using dot blot analysis. The antigen for this polyclonalantibody was made from each of the E. tenella stadium by sonication. Fifteen mice, divided into 3 groups,were then injected subcutaneously with each antigen. The sera from these mice were then collected, analyzedby using ELISA and then it will be used for the dot blot analysis. The research result showed that thepolyclonal antibody which has been developed in mice from each antigen can react with the antigen itself.From this result it can be concluded that the developing of this antibody is successful and it can be used forfurther research in immunoproteomic.
Identifikasi Keragaman Gen Toll-Like Receptor-4 Ayam Lokal dengan Polymerase Chain Reaction- Restriction Fragment Lenght Polymorphism (THE GENETIC POLIMORPHISM OF TOLL-LIKE RECEPTOR-4 GENE IN LOCAL CHICKENS USING POLYMERASE CHAIN REACTION-RESTRICTION FRAG Niken Ulupi; Muladno .; Cece Sumantri; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (161.852 KB)

Abstract

Toll-like Receptor 4 (TLR4) gene is one of the genes that control resistance of chickens againstSalmonella sp. through non-specific immune response. These gene can be used as genetic markers inIndonesian local chickens, if known its diversity. Therefore this research was aimed at evaluating thegenetic polymorphism of TLR4 gene in several types of Indonesian lokal chickens (kampung, kampungpelungcrossbreed, sentul, and tolaki chicken) using PCR-RFLP. The total of samples were 136. There werethree stages of identification : extraction of DNA genom, PCR amplification of TLR4 gene (with size 220 bpon exon 2), and RFLP method using restriction enzym (MscI). The data were analyzed include frequency ofallele, frequency of genotype, heterozygosity values , and value of Polymorphic Informative Content (PIC).The result showed that TLR4|MscI was found polymorphic in all kind of chickens that was genotyped. Itwere found two alleles (A and G), and three genotypes (AA, AG, and GG). The value of x2 showed thatTLR4|MscI was in Hardy-Weinberg equilibrium. The value of Ho and He were 0,11-0,41 and 0,11-0,35. Thevalue of PIC (0,10-0,29) included in the category of low to moderately high. These results declared thatTLR4|MscI gave hope for used as genetic markers in resistance to Salmonella sp. infection in Indonesianlocal chickens.
Gambaran Patologi Bursa Fabricius Embrio Ayam Pascavaksinasi Gumboro Secara In Ovo Menggunakan Vaksin Lokal dan Komersial (PATHOLOGIC DESCRIPTION OF BURSA FABRICIUS CHICKEN EMBYROS AFTER IN OVO VACCINATED WITH LOCAL AND COMMERSIAL GUMBORO VACCINES) Sutiastuti Wahyuwardani; Dewi Ratih Agung Priyono; Wasmen Manalu
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.094 KB)

Abstract

Bursa Fabricius is a target organ of gumboro virus infection which is often damaged after vaccinationusing hot intermediate gumboro live vaccine. The purpose of this study was to assess pathologic effect oflocal and commercial gumboro vaccines . As many as 45 embryo chicken eggs at nine day old were used inthis research, then grouped into three groups of 15 embryos chicken eggs each, these were: Embryo chickeneggs without vaccination (Group I), vaccinated with IBD intermediate plus commercial vaccine (Group II)and IBD intermediate plus local vaccine (Group III). Vaccinations were done at 14 days old. All groups thenterminated each three embryos at 12 hours, 1, 2, 3 days post vaccination. The results showed that pathologicanatomic lesions could not be detected. Whereas pathologic lesions were detected in the group that werevaccinated with intermediate plus local IBD observed more severe than in the group that vaccinated withintermediate plus commercial IBD. Lesions such as edema, hemorrhages, necrosis of lymphoid cells wereobserved microscopically in embryo at 12 hours, 1, 2 and 3 days post vaccination in Group II and group III.The lesions were more severe at two days post vaccination causing some lymphoid follicles disappeared at three days post vaccination. However, they were not detected again in the bursa Fabricius three days afterhatching. Cells containing antigens of gumboro were detected in the bursa Fabricius of chicken embryo atone day until three days post vaccination, then disappeared after three days post hatch. It was concludedthat pathologic description of bursa fabricius showed that virus vaccines used for vaccinated IBD in ovowere still virulent, that can cause histopathologic lesions. The viruses are suggested to be more attenuatedbefore using as vaccine in ovo.
Botulismus pada Manusia (BOTULISM IN HUMAN) I Wayan Suardana
Jurnal Veteriner Vol 2 No 1 (2001)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5379.061 KB)

Abstract

Botulismus pada Manusia   (BOTULISM IN HUMAN)
Prevalensi dan Faktor Risiko Infeksi Dirofilaria immitis pada Anjing yang Dipotong di Daerah Istimewa Yogyakarta (PREVALENCE AND RISK FACTOR OF THE DIROFILARIA IMMITIS INFECTION IN DOGS SLAUGHTERED IN DAERAH ISTIMEWA YOGYAKARTA) I G. Made Krisna Erawan; Ida Tjahajati; Wisnu Nurcahyo; Widya Asmara
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (98.758 KB) | DOI: 10.19087/jveteriner.2017.18.4.541

Abstract

The aim of this study was to determine the prevalence and risk factors Dirofilariaimmitis (D. immitis) infection in dogs slaughtered in Yogyakarta. A total of 151 dogs that were slaughtered from May – November 2013 were examined their heart in order to determine the presence of D. immitis infection. Blood samples were tested using Modified Knott’s Technique for microfilariae examination. The results showed that based on the heart and blood examination the prevalence of D. immitis infection was 14.6 % and 7.9 %, respectively. The risk factors for D. immitis infection were the age and origin of the dog.
Keragaman Endoparasit pada Macaca fascicularis dan Potensi Zoonotiknya dengan Cuaca Berbeda di Kota Kupang (ENDOPARASITIC DIVERSITY IN MACACA FASCICULARIS AND ITS ZOONOTIC POTENTIAL ON DIFFERENT WEATHER IN KUPANG CITY) Jayusman Arsiyanti Joesoef; Dondin Sajuthi; Agus Wijaya; Maxs Urias Ebenhaizar Sanam
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.564 KB) | DOI: 10.19087/jveteriner.2018.19.4.451

Abstract

Long-tailed macaque (Macaca fascicularis) belongs to least concern categories of animals, and often found in various regions of Indonesia, including Kupang, East Nusa Tenggara. Their habitat are one of the tourist destinations, causing interaction between them and humans. The interaction facilitates the transmission of disease agents, for example endoparasit. This study was conducted to find out the diversity and prevalence of endoparasites in long-tailed macaque, weather effects, and zoonotic potential in Kupang City. Fifty fecal samples were collected and analyzed using formalin ethyl acetate concentration technique (FECT). A total of six endoparasites were recovered including four helmints (hookworm, Strongyloides, Toxocara, and Acantocephala) and two protozoans (Balantidium coli and Entamoeba sp.). The highest prevalence of infection occurred in February (rainfall 302.4 mm) and consisted of hookworm (86%) and B. coli (66%). If rainfall increases, prevalence will also increase. The high prevalence of parasites is not always associated with the appearance of clinical symptoms. An individu diagnosed with parasites if the eggs or cysts have been identified from the faeces. The high prevalence caused by the absence of deworming program. Among the endoparasites that have been found, some of which are potentially zoonotic, such as: Strongyloides sp., Toxocara sp., Acanthocephala, B. coli, and Entamoeba sp.
Derajat Pemulihan dan Persentase Spermatozoa X dan Y Kambing Peranakan Etawah Setelah Separasi dengan Gradient Percoll (RECOVERY RATE AND PERCENTAGE OF SPERMATOZOA X AND Y OF ETAWAH CROSSBREED GOAT AFTER SEXING WITH GRADIENTT PERCOLL) Siti Darodjah Rasad; Rangga Setiawan; Nurcholidah Solihati; Rini Widyastuti; Ilham Nugraha
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.116 KB) | DOI: 10.19087/jveteriner.2019.20.1.14

Abstract

Aim of the research is to find out recovery rate and percentage spermatozoa X and Y after sexing with gradientt Percoll in Etawah Crossbreed Goat. Extender was used TRIS Egg Yolk. The semen sample from five male Etawah Crossbreed Goat were used in this research. Gradientt percoll performed 10 fraction layer with centrifugation time 10 minute at 2500 rpm. The data were analyzed descriptively. Result of the research shows that average recovery rate of spermatozoa X is 50.96±26.07 % and spermatozoa Y is 44.13±6.11 %. Average percentage of X sperm is 78.00±7.06 % and Y sperm is 76.10±5.95 % .

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