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I G. Made Krisna Erawan
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Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia
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INDONESIA
Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
Diagnosis Feline Panleukopenia Berdasar Total Leukosit dan Uji Feline Parvovirus-Antigen pada Kucing-Kucing Diare Hary Purnamaningsih; Soedarmanto Indarjulianto; Yanuartono Yanuartono; Alfarisa Nururrozi; Irkham Widiyono; Slamet Rahardjo; Sri Hartati; Rusmihayati Rusmihayati
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (262.718 KB) | DOI: 10.19087/jveteriner.2022.23.1.36

Abstract

Feline panleukopenia (FPL) is one of the most common disease that causes diarrheal and leukopenia in cats. The purpose of this study was to diagnose FPL based on total leukocyte and detection of feline parvovirus antigen (FPV-Ag) in diarrhea cats. This study used blood samples and feces swabs from 21 cats that showed symptoms of diarrhea. Total leukocytes are calculated from blood samples and the presence of FPV antigens is detected from feces swabs using FPV-Ag-kit. The suitability of the FPL diagnosis based on leukopenia and a positive reaction to the FPV-Ag test was compared using the Cohen’s Kappa suitability test. The results showed that 14 out of 21 cats (66.7%) had leukopenia and 7 of 21 cats (33.3%) had normal total leukocytes. Results of FPV-Ag test showed that 11 of 21 cats (52%) were positive and 10 of 21 cats (48%) were negative. The both test has a good agreement with a 0.71 conformity value. Based on this study it was concluded that the diagnosis of FPL based on total leukocyte and FPV-Ag have a good agreement, so that both tests could be used as a basis for FPL diagnosis.
Bisphenol A Meningkatkan Malondialdehid dan Indeks Apoptosis Hati Tikus (Rattus norvegicus) Jantan Risha Catra Pradhany; I Nyoman Suarsana; I Gusti Ayu Agung Suartini; Ferbian Milas Siswanto
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.285 KB) | DOI: 10.19087/10.19087/jveteriner.2022.23.1.80

Abstract

Bisphenol-A (BPA) merupakan toksikan yang diketahui dampaknya terhadap reproductive toxicities.Namun, beberapa tahun belakangan ini, diketahui pula bahwa BPA menyebabkan stres oksidatif. Stres oksidatif merupakan salah satu faktor penyebab kerusakan organ. Penelitian ini bertujuan untuk membuktikan efek pemberian BPA oral terhadap kadar malondialdehid dan indeks apoptosis pada hati tikus. Penelitian ini menggunakan posttest only control group design. Subjek adalah 14 ekor tikus putih jantan galur Sprague dawley, umur 8-10 minggu, bobot badan berkisar 180 g, dan dalam keadaan sehat. Kelompok kontrol (P0), tujuh ekor tikus, diberikan plasebo berupa 1 mL aquadest selama 21 hari; sedangkan kelompok perlakuan (P1), tujuh ekor tikus, diberikan 400 mg/kgBB tikus BPA selama 21 hari. Hasil menunjukkan bahwa kelompok P1 memiliki kadar MDA hepatik yang lebih tinggi (3,33±0,27 nmol/ mg.prot) dan berbeda nyata (p<0,001) dibandingkan kelompok P0 (2,67±0,14 nmol/mg.prot). Selain itu, kelompok P1 memiliki indeks apoptosis yang lebih tinggi (11,21±2,26%) dan berbeda nyata (p<0,001) dibandingkan kelompok P0 (2,19±0,97%). Berdasarkan hasil tersebut, dapat disimpulkan bahwa pemberian BPA oral meningkatkan malondialdehid dan indeks apoptosis pada hati tikus putih (Rattus norvegicus) jantan.
Aktivitas Ovisidal, Larvasidal dan Vermisidal Ekstrak Obat alami Terhadap Nematoda Haemonchus contortus secara in-Vitro Fitrine Ekawasti; Dias Aprita Dewi; Suhardono Suhardono; Dyah Haryuningtyas Sawitri; Eny Martindah; April Hari Wardhana
Jurnal Veteriner Vol 23 No 2 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.523 KB) | DOI: 10.19087/jveteriner.2022.23.2.146

Abstract

Hemonchosis disebabkan oleh cacing nematoda Haemonchus contortus dan dapat menyebabkan kerugian ekonomi yang tinggi. Penanganan dan pengendalian kecacingan yang paling umum dilakukan adalah dengan pemberian antelmintika kimia berspektrum luas, tetapi jika digunakan secara terus menerus dalam waktu yang lama dapat mengakibatkan resistensi dan residu terhadap produk ternak yang menjadi ancaman serius bagi produksi ternak. Penelitian ini bertujuan untuk mendeteksi apakah tanaman herbal biji pare (Momordica charantia L.), temu putih (Curcuma zedoaria Rosce), buah mengkudu (Morinda citrifolia), biji alpukat (Persea americana mill), buah pinang (Areca catechu) dapat digunakan sebagai obat cacing nematoda yang efektif dan efisien. Tanaman herbal diekstrak dan diuji secara fitokimia di Balai Penelitian Rempah dan Obat (Balittro). Ekstrak biji pare dan biji alpukat dalam DMSO 1% dengan konsentrasi 2.5%, 5% dan 7.5% memiliki aktivitas antelmintik ovisidal, larvasidal dan vermisidal.
Deteksi Faktor Virulensi Secara Fenotip pada Escherichia coli Isolat Susu Mentah Sapi Perah Khusnan Khusnan; Agus Purnomo
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.451 KB) | DOI: 10.19087/jveteriner.2022.23.1.42

Abstract

Susu sapi merupakan media pertumbuhan bakteri patogen maupun non patogen. Escherichia coli mrupakan salah satu bakteri yang sering ditemukan pada susu sapi mentah. Escherichia coli patogen pada sapi dapat menyebabkan mastitis maupun radang usus serta dapat menyebabkan masalah kesehatan pada manusia seperti diare, kolitis hemoragik dan sindrom uraemik hemolitik. Hemaglutinin dan hemolisin merupakan faktor virulen yang penting pada E. coli patogen, karena berperan sebagai faktor adhesin, dan faktor pertahanan terhadap fagositosis. Tujuan penelitian ini mendeteksi hemaglutinin dan hemolisin E. coli isolat asal susu sapi mentah serta kemampuan adhesi pada sel epitel serta pertahanan terhadap fagosit netrofil. Hasil penelitian ini menunjukkan 100% isolat E. coli tidak memiliki hemaglutinin dan 17,2% merupakan E. coli hemolitik. Pada uji adhesi mampu melekat pada sel epitel bukalis 46,87 bakteri/sel. Pada uji fagositosis isolat-isolat hemolitik lebih sedikit difagosit oleh neutrofil dibandingkan isolat non hemolitik (2,64 dibanding 3,1 bakteri) tiap neutrofil (P<0,05). Ditemukannya E. coli patogen pada susu mentah menegaskan pentingnya pasteurisasi pada susu sebelum dikonsumsi.
Keberhasilan Hiperimunisasi Pada Sapi Bunting: Kajian Terhadap Ketepatan Waktu Vaksinasi Safitria Wulandari; Anita Esfandiari; Sri Murtini; Retno Wulansari
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (598.565 KB) | DOI: 10.19087/jveteriner.2022.23.1.88

Abstract

Penelitian ini bertujuan untuk melihat pengaruh umur kebuntingan induk sapi saat vaksin pertama kali diberikan terhadap keberhasilan hiperimunisasi. Sebanyak 12 ekor induk sapi bunting jenis sapi Friesian Holstein, dibagi menjadi kelompok kontrol dan vaksin, dengan masing-masing kelompok terdiri dari umur kebuntingan enam bulan (n=3) dan tujuh bulan (n=3). Induk sapi diinjeksi vaksin Avian influenza (AI) H5N1 komersial (killed vaccine) sub-kutan, sebanyak 5 dosis/ekor (106 EID50/dosis). Vaksinasi dilakukan dua kali dengan interval antar vaksinasi selama dua minggu. Sebelum vaksinasi, induk sapi diberi imunomodulator 0,1 mg/kg bb per oral selama tiga hari berturut-turut kemudian dilakukan priming dengan menyuntikkan antigen AI H5N1 inaktif tanpa adjuvant (intravena) tiga hari berturut-turut (10x28 HAU/ekor). Sampel darah dikoleksi melalui vena coccygea sebelum vaksinasi pertama, dua minggu pascavaksinasi I dan dua minggu pascavaksinasi II untuk dianalisis terhadap parameter leukosit (jumlah total leukosit dan limfosit), kadar total protein, globulin dan rasio albumin/ globulin (A/G), serta titer IgG anti AI H5N1 dalam darah dan kolostrum. Hasil pengamatan menunjukkan tidak terdapat perbedaan yang signifikan pada jumlah total leukosit dan limfosit, kadar total protein dan globulin, rasio A/G, serta titer IgG anti AI H5N1 dalam darah dan kolostrum, baik antar kelompok maupun antar waktu pengamatan (p>0.05). Namun demikian, pada induk sapi kelompok vaksin umur kebuntingan enam bulan menunjukkan parameter leukosit, kadar total protein dan globulin serta titer IgG anti AI H5N1 dalam darah dan kolostrum lebih tinggi dibandingkan kelompok induk sapi umur kebuntingan tujuh bulan. Umur kebuntingan pada saat vaksinasi pertama kali dilakukan tidak memengaruhi keberhasilan hiperimunisasi pada induk sapi bunting trimester akhir.
Intensitas Latihan Fisik Ringan-Berat Kurang Memengaruhi Kadar Follicle Stimulating Hormone Serum Mencit Betina Anggis Putri Wijayanti; Lilik Herawati; Endyka Erye Frety; Zakiyatul Faizah
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (537.048 KB) | DOI: 10.19087/jveteriner.2022.23.1.10

Abstract

Physical exercise can cause a change in hormonal system resulting an irregularities of menstrual cycle. Menstrual irregularities is one of the ovulation disruption symptoms. The most important cause of ovulation disruption is a decrease of Gonadothropin Releasing Hormone (GnRH) which is regulated by hipothalamus-pitutary-ovarium axis (HPO axis) mechanism resulting in decreased of Follicle Stimulating Hormone (FSH) secretion. HPO axis mechanism disruption can be caused by severe physical activity. Methods: This research took place in phaculty of veterinary of Airlangga University. The methods of this research was experimental laboratory study with Randomized Post-test Only Control Group Design. The number of sample were 28 female mice divided into four groups with a sampling technique using probability sampling with the type of simple random sampling. The independent variable of this research were moderate and severe physical exercise. The dependent variable were serum Follicle Stimulating Hormone (FSH) levels. The data were analyzed using SPSS 25 software with one way- ANOVA test. This research was conducted to analyze the difference effect of low to severe physical exercise on serum Follicle Stimulating Hormone (FSH) levels in female mice. The result is there were no significant difference in levels of Follicle Stimulating Hormone (FSH) mice with low, moderate and severe physical activity. The conclusion that can be taken in this research is there is no difference in the effect of low, moderate to severe physical exercise on serum Follicle Stimulating Hormone levels in female mice.
Deteksi Gen Penyandi Resistansi Tetracycline dan Plasmid Mediated Quinolones pada Salmonella Ayam di Bandung dan Purwakarta Leila Nur Azizah; Agustin Indrawati; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (644.554 KB) | DOI: 10.19087/jveteriner.2022.23.1.55

Abstract

This study aimed to identify genes encoding tetracycline and plasmid-mediated quinolones resistance to Salmonella spp from Poultry Farm in Bandung and Purwakarta, West Java. A total of 70 samples were collected from poultry farm In Bandung and Purwakarta, West Java. All isolates were test by selective media (SSA) and confirmation Salmonella with PCR. Thirty three isolate positive from selective media Salmonella Shigella Agar (SSA) and 21 isolat was confirmed as Salmonella spp by PCR. Twenty one isolate isolated were tested for tetracycline, doxycicline,, nalidixic acid, oxytetracycline, enrofloxacyn using disk diffusion method. TE-resistant were screened for presence of tet(A) and tet(B) genes by single polymerase chain reaction (PCR). The qnr(A), qnr(B) and qnr(S) genes were detected by multiplex PCR in quinolone resistant Salmonella isolates. The result of antibiotic sensitivity test showed that resistance to ampicillin (95.2%), tetracycline (100%), oxytetracycline (95.2%), nalidixic acid (90.4%), eritromisin (85.7%), enrofloxacin (76.2%), Gentamisin 47.6%, chloramphenicol (38.1%). The distribution of antibiotics-resistance genes in the Salmonella isolates included ampC (95.2%), tet(A)(61.9%), tet(B)(38.1%), qnr(A)(28.5%), qnr(B)(14.3%) and qnr(S)(23.8%).This study shows that a few pathogens of Salmonella are resistant to ampicillin, tetracycline , and quinolone. The tet and qnr genes are responsible for this resistance among Salmonella in Bandung and Purwakarta, West Java Indonesia was high.
Morfologi dan Morfometri Sel Darah Rusa Timor (Rusa timorensis) di Penangkaran Virgilius Martin Kelake Kedang; Anita Esfandiari; Arief Purwo Mihardi; Robby Wienanto
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (221.145 KB) | DOI: 10.19087/jveteriner.2022.23.1.99

Abstract

Rusa timor (Rusa timorensis) merupakan salah satu jenis rusa asli Indonesia. Salah satu aspek penting dalam upaya konservasi adalah terkait aspek kesehatan rusa timor. Nilai normal maupun gambaran rujukan terkait karakteristik fisiologis hewan dinilai penting dalam mengevaluasi status kesehatan rusa timor di penangkaran. Salah satu aspek yang penting untuk dipelajari yaitu karakteristik morfologi sel darah. Penelitian ini bertujuan mengetahui morfologi dan morfometri sel darah rusa timor di penangkaran. Sampel darah diperoleh dari 10 ekor rusa timor yang terdiri dari lima ekor rusa timor jantan dan lima ekor rusa timor betina. Sampel darah dipreparasi dalam sediaan ulas darah dengan pewarna Giemsa kemudian diamati di bawah mikroskop. Pengukuran morfometri sel darah dilakukan menggunakan mikroskop digital. Morfologi eritrosit rusa timor pada sediaan ulas darah memperlihatkan adanya poikilositosis berupa sel sabit. Morfometri eritrosit rusa timor jantan secara nyata (P<0,05) lebih besar dibandingkan dengan rusa timor betina. Gambaran morfologi leukosit rusa timor sama dengan leukosit mamalia pada umumnya. Morfologi dan morfometri masing-masing jenis leukosit rusa timor tidak memiliki perbedaan nyata (P>0,05) antar jenis kelamin
Analisis Infektivitas Simian-Human Immunodeficiency Virus pada Peripheral Blood Mononuclear Cell Macaca fascicularis dan M. nemestrina Secara In Vitro Gede Eko Darmono; Silvia Triwidyaningtyas; Budiman Bela; Diah Iskandriati; Dondin Sajuthi; Joko Pamungkas
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (321.183 KB) | DOI: 10.19087/jveteriner.2022.23.1.16

Abstract

Development of human immunodeficiency virus type 1 (HIV-1) vaccines and anti-retroviral treatmentis currently hindered by the lack of models representing prominent symptoms of HIV-1 infections seen in humans. Simian-human immunodeficiency virus (SHIV) was constructed to resolve the limitations of SIVmac model and has been used in nonhuman primate model ofviral infections, particularly infections by the close relatives of HIV-1. Macaca fascicularis and M. nemestrina are being developed as model HIV/AIDS, by using chimeric virus SHIV produced by replacing the nucleotide structure of cyclophilin A binding region, vif gene and nef of HIV-1 with cyclophilin A binding region, vif gene and nef from SIV. The research aims to study the model of HIV/AIDS on nonhuman primates PBMC in vitro using SHIV. In particular, the study aims to obtain information about the capability of SHIV replication in PBMC of M. fascicularis and M. nemestrina. Results showed a cytopathic effect (CPE) in the form of multinucleated giant cells and expression of p24 protein in PHA-stimulated PBMC cultures of M. fascicularis and M. nemestrina after SHIV infection. The conclusion of this study is that SHIV can infect PBMC M. fascicularis and M. nemestrina in vitro based on CPE and expression of p24 protein.
Peningkatan Performa Reproduksi Induk Babi Melalui Pengaturan Penyapihan Anak Babi Ni Luh Gde Sumardani; Komang Budaarsa; Anthonius Wayan Puger
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (22.718 KB) | DOI: 10.19087/jveteriner.2022.23.1.64

Abstract

This study aims to determine the optimal timing of weaning piglets from the sows so that the sows can return to estrus, thereby increasing the efficiency of production and reproduction of these sows. The design used a randomized block design (RBD) with three treatments, the piglets weaned at 25 days (A), 28 days (B), and 30 days (C) in three pig farms. Each treatment consisted of three replications, and each replication consisted of two of the sow. The variables observed: litter size, bodyweight of weaned piglets, estrus after weaning, and the percentage of mortality of piglets after weaning. The results showed that the bodyweight of the weaning piglets and the mortality after weaning were 6.83 kg; 1.33% (A), 8.17 kg; 1.17%(B), and 9.0 kg; 1.17% (C) significant (P<0.05) in treatment B; The sows were on estrus after weaning an average of 3.67 days (A and C) and 3.33 days (B) was nonsignificant (P>0.05). The litter size reaches 10.17 head (A), 13.0 head (B) and 11.5 head (C) were nonsignificant (P>0.05). The conclusion of this research is the optimum time for the implementation of weaning piglets is 28 days old in the pig farming business.

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