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INDONESIA
The Indonesian Biomedical Journal
Published by The Prodia Education and Research Institute
ISSN : -     EISSN : -     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Public Health
Arjuna Subject : -
Articles 621 Documents
 54   55   56   57   58 
Lower Plasma β-Amyloid 1-42 Levels in Amnestic Mild Cognitive Impairment Compared to Healthy Individuals Cynthia Cynthia; Jusak Nugraha; Muhammad Hamdan; Hari Basuki Notobroto; Silvia Francina Lumempouw; Rahajuningsih Dharma
The Indonesian Biomedical Journal Vol 16, No 6 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i6.3404

Abstract

BACKGROUND: Amnestic mild cognitive impairment (aMCI) is strongly associated with an increased risk of progression to Alzheimer’s disease (AD). In AD, cerebrospinal fluid (CSF) β-Amyloid 1-42 levels are known to decrease, a pattern which is also observed in aMCI. While in AD, apolipoprotein E (ApoE) ε4 allele is known to be a genetic risk factor, the role of ApoE ε4 allele in modulating plasma β-Amyloid 1-42 levels in aMCI remains unclear. Therefore, this study was performed to evaluate plasma β-Amyloid 1-42 levels in aMCI patients compared to cognitively healthy individuals and investigate its association with ApoE ε4 allele.METHODS: A cross-sectional study involving 57 aMCI and 54 cognitively healthy control (HC) subjects was performed. Blood samples were taken from subjects from both groups for measurement of the plasma β-Amyloid 1-42 and ApoE ε4 allele. The plasma levels of β-Amyloid 1-42 were measured using an enzyme-linked immunosorbent assay (ELISA) methods, while the ApoE ε4 allele genotyping was conducted using polymerase chain reaction (PCR) techniques.RESULTS: Plasma β-Amyloid 1-42 in individuals with aMCI (23.9 pg/mL) was significantly lower than that in HC (25.3 pg/mL) with cut-off value of 24.6 pg/mL (AUC: 70.8%; 95% CI: 61.1–80.5%; p<0.001) sensitivity of 64.8%, and specificity of 71.9%. There was no significant association between plasma β-Amyloid 1-42 and the ApoE ε4 allele. However, plasma β-Amyloid 1-42 in ε4 carriers were lower than in ε4 non-carriers.CONCLUSION: Lower plasma β-Amyloid 1-42 levels were observed in aMCI patients compared to cognitively healthy individuals, suggesting its potential as a biomarker for identifying aMCI.KEYWORDS: blood biomarkers, amyloid beta peptides, amnestic mild cognitive impairment (aMCI)
Mesenchymal Stem Cell–derived Extracellular Vesicles: An Emerging Therapeutic Strategy for Diabetic Wound Healing Meiliana, Anna; Dewi, Nurrani Mustika; Wijaya, Andi
The Indonesian Biomedical Journal Vol 16, No 6 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i6.3407

Abstract

One of the most serious side effects of diabetes is diabetic foot ulceration (DFU). It is a severe and extremely morbid illness that has been linked to higher mortality on its own. The development of effective wound therapeutics in the future may be influenced by our current and developing understanding of wound pathophysiology. By reestablishing cellular functioning, small extracellular vesicles (sEVs), a crucial medium for intercellular communications, exhibit encouraging therapeutic potential in the treatment of DFU. Mesenchymal stem cell (MSC) derived exosomes and engineered extracellular vesicles (EVs) have the potential to aid in the healing of wounds. Along with encouraging the growth and stimulation of endothelial cells, keratinocytes, and fibroblasts, they also have immunomodulatory and anti-inflammatory properties. They help prevent damaged cells from dying, revitalize senescent cells, and boost angiogenesis. MSC-EVs can be a safe, effective and ethical therapy for DFU by increasing M2 macrophages polarization, improving the proliferation, reducing scar, and improving angiogenesis.KEYWORDS: mesenchymal stem cell, extracellular vesicle, diabetic wound, wound healing
Molecular Mechanisms of Methylglyoxal in Diabetes-related Macrovascular Complications Meiliana, Anna; Dewi, Nurrani Mustika; Wijaya, Andi
The Indonesian Biomedical Journal Vol 16, No 5 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i5.3242

Abstract

Diabetes mellitus (DM) is a chronic endocrine and metabolic disease indicated by the presence of hyperglycemia. It has been known that hyperglycemia and oxidative stress are the main culprit of all DM complications, including macrovascular complications. As a byproduct of lipid, protein, and carbohydrate metabolism, methylglyoxal (MGO) is a highly reactive substance which plays a positive signaling role in helping cells regain redox balance under oxidative stress circumstances. DM-related problems lead to an excess of mitochondrial superoxide in the heart and big and small vascular endothelial cells. Elevated intracellular reactive oxygen species induce impaired angiogenesis in reaction to ischemia, trigger several proinflammatory pathways, and result in enduring epigenetic modifications that propel the continuous expression of proinflammatory genes even after glucose levels return to normal. Over time, the significance of the extremely quick advanced glycation end-products (AGE) production caused by the extremely reactive MGO has been clarified. It is now evident that MGO causes vascular tissue to react maladaptively. Glyoxalase 1 (GLO1) is the primary enzyme in an organism's enzymatic glyoxalase defense mechanism, which converts MGO to D-lactate in order to counteract the harmful effects of MGO. Understanding the role of the MGO–GLO1 pathway in the etiology of vascular disease in diabetes has advanced significantly. Therefore, it can be summarized that vascular damage are linked to diabetes. The AGE precursor MGO are important in determining the connection between diabetes and vascular damage. MGO and AGEs play a role in several phases of the development of diabetes complications. MGO and AGEs may be useful therapeutic targets for diabetes's macrovascular problems.KEYWORDS: hyperglycemia, AGE, methylglyoxal, glyoxalase, D-lactate, gluthatione, oxidative stress
Red Rice Bran Ethanol Extract Reduces IL-1β as the Risk of Pancreas Fibrogenesis in Type 2 Diabetic Rat Model Brian Wasita; Ratih Dewi Yudhani; Dyonisa Nasirochmi Pakha; Diana Nurrohima; Muthmainah Muthmainah; Nanang Wiyono; Shariza Fakurazi
The Indonesian Biomedical Journal Vol 17, No 1 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i1.3410

Abstract

BACKGROUND: Oxidative stress and inflammation contribute to pancreatic cell dysfunction that promote insulin resistance in type 2 diabetes (T2D). Red rice bran contains bioactive substances with anti-inflammatory and antioxidant properties which improved insulin resistance in obese mice. However, no studies have explored the potential of ethanol extract of red rice bran (EERRB) to prevent T2D progression, particularly pancreatic fibrosis complications. This study was conducted to investigate the effect of EERRB on inflammation measured with interleukin (IL)-1β and fibrosis of pancreatic tissue in a rat model of T2D. METHODS: Rats were induced with streptozotocin and nicotinamide to induce diabetes, and then separated into five groups. One group received no treatment, while the other four received 9 mg/kg/day acarbose, 165, 330, or 660 mg/kg/day EERRB orally for 21 days. Immunohistochemistry was conducted on pancreas tissues to measure the expression of IL-1β, while pancreatic fibrosis was assessed with Masson’s Trichrome staining.RESULTS: EERRB reduced the expression of pro-inflammatory cytokine, IL-1β, in pancreas tissue in a dose dependent manner. Significantly lower IL-1β expression were found in group receiving 660 mg/kg/day EERRB (10%) compared to diabetic with no treatment group (50%) (p<0.0001). Additionally, the IL-1β expression in the highest dose of EERRB group was comparable to the group receiving acarbose (10%). CONCLUSION: This finding suggests the beneficial effect of EERRB in the hyperglycemic condition that causes oxidative stress through blocking the IL-1β expression, hence alleviating the inflammation in pancreas tissue, and have a tendency in preventing pancreatic fibrosis progression, a process implicated in T2D pathogenesis. KEYWORDS: diabetes, inflammation, pancreatic fibrosis, red rice bran
Lactococcus lactis D4 Decreases NF-κB and α-SMA in Rat Models of Obstructive Jaundice Suchitra, Avit; Alvarino, Alvarino; Darwin, Eryati; Harun, Harnavi; Rivai, Muhammad Iqbal; Sukma, Ade
The Indonesian Biomedical Journal Vol 16, No 6 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i6.3384

Abstract

BACKGROUND: Obstructive jaundice, often due to choledocholithiasis or malignancies, leads to immune suppression, intestinal damage, and bacterial translocation, worsening outcomes. Some inflammatory mediators like nuclear factor kappa B (NF-κB), alpha-smooth muscle actin (α-SMA), and interleukin-6 (IL-6) are important in this process. Current treatments remain inadequate, highlighting the need for novel approaches. Probiotics, such as Lactococcus lactis D4 (LLD4), may help reduce inflammation and bacterial translocation, thus offering a potential therapeutic option. This study was conducted to evaluate the effects of LLD4 on NF-κB, α-SMA, and IL-6 in obstructive jaundice rat models.METHODS: This post-test randomized controlled study involved 15 male Wistar rats divided into three groups: sham, bile duct ligation (BDL), and BDL+LLD4 groups. The rats were maintained for 7–10 days, with the rats in BDL+LLD4 group received fermented milk containing LLD4 via gavage at a dose of 112 mg/20 gBW per day for 7 days. The expression levels of NF-κB, α-SMA, and IL-6 were analyzed using immunohistochemistry.RESULTS: Administration of LLD4 were able to significantly reduced NF-κB expression compared to the BDL group (40.20±21.276 vs. 53.60 ± 20.403) in obstructive jaundice rat models. Though not significant, BDL+LLD4 group showed lower α-SMA expression compared to BDL group (58.40±14.271 vs. 63.20±9.16). However, administration of LLD4 did not give any significant effect on IL-6 expression.CONCLUSION: LLD4 reduces inflammation in models of obstructive jaundice by lowering the NF-κB and α-SMA expression. This indicates that LLD4 might be potential as an adjunct therapy for reducing morbidity in obstructive jaundice cases.KEYWORDS: obstructive jaundice, bile duct ligation, Lactococcus lactis D4, NF-κB, α-SMA, IL-6
Black Rice Extract Reduces Body Weight, Waist Circumference, Body Mass Index and Lipopolysaccharide in Obese Subjects: A Preliminary Study Makmun, Armanto; Bukhari, Agussalim; Taslim, Nurpudji Astuti; Aminuddin, Aminuddin; Sandra, Ferry
The Indonesian Biomedical Journal Vol 16, No 5 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i5.3250

Abstract

BACKGROUND: The prevalence of obesity, or an excessive fat accumulation, is keep increasing. In obesity, inflammation can be induced by leaky gut due to the intestinal tight junction barrier dysfunction. Zonula occludens-1 (ZO-1) plays a role in developing intestinal tight junction barrier dysfunction and gut microbiota imbalance, thus promote the translocation of bacterial endotoxin characterized by lipopolysaccharide (LPS) into circulation. Black rice extract (BRE) has been known to have anti-inflammatory property. This study was conducted to investigate the effect of BRE on body weight (BW), waist circumference (WC), body mass index (BMI), ZO-1 and LPS of obese patients.METHODS: Twenty-three male subjects were divided into non-obese group (NOG), obese group (COG) and BRE-obese group (BOG). Subjects in BOG received a daily dose of 5.6 g/day BRE for 4 weeks. BW, WC and BMI, serum ZO-1 and LPS were measured before and after treatment.RESULTS: BRE was prepared successfully and free from microbial contamination. Treatment of BRE for 4 weeks reduce BW (95.40±5.78 vs. 94.59±6.00 kg, p=0.043), WC (109.25±3.55 vs. 107.50±3.46 cm, p=0.000) BMI (32.65±1.86 vs. 32.18±1.80, p=0.000) and LPS (222.27±38.63 vs. 131.63±9.70 ng/mL, p=0.020) of obese subjects. The pre-post ZO-1 levels in all groups were not significantly different (p>0.05).CONCLUSION: Treatment of 5.6 gr BRE daily for four weeks can reduce BW, WC, BMI and serum LPS, but not serum ZO-1 in obese patients. Therefore, BRE may reduce inflammation in obesity.KEYWORDS: black rice, obesity, BW, WC, BMI, LPS, ZO-1
Lacticaseibacillus rhamnosus Probiotics Improve Fasting Blood Glucose, HOMA-IR, and Reduce Body Weight in Diabetic Rat Model Arresta Vitasatria Suastika; I Gde Raka Widiana; Anak Agung Ngurah Jaya Kusuma; Ni Nengah Dwi Fatmawati; Ketut Suastika; I Nengah Sujaya
The Indonesian Biomedical Journal Vol 17, No 1 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i1.3525

Abstract

BACKGROUND: Indonesian indigenous probiotics have been found to improve disruptions of tight junctions in the intestinal epithelium and reduce total cholesterol levels. Improvement in the tight junction could decrease the LPS level and further reduce the blood glucose and insulin resistance. The effects of indigenous Indonesian Lacticaseibacillus rhamnosus (Lr) probiotics on glucose metabolism and inflammatory marker levels in diabetic rats was studied to find if these probiotics are suitable as potential supplementation treatment in diabetes.METHODS: Sixteen female Wistar rats were induced with diabetes using streptozotocin and fed a high-fat, high-sucrose diet. The rats were separated into four groups: LrFBB81, LrFSMM22, LrSKG34, and a control group. Each intervention group got daily dosages of 1 mL probiotic suspensions containing 109 CFU/mL cells given orally for 14 days, whereas the control group received saline. Fasting blood glucose (FBG), insulin, homeostatic model assessment for insulin resistance (HOMA-IR), lipopolysaccharide (LPS), and body weight were evaluated.RESULTS: FBG was significantly reduced in LrFSMM22 group (Δ=120.75 mg/dL, p=0.035), while significant reduction was not observed from LrFBB81, LrSKG34, and control groups. No statistically significant differences were found in HOMA-IR before and after intervention in all groups, but Δ HOMA-IR from LrFSMM22 group was reduced more than the control group (-3.90 vs. 2.02, p=0.028). All groups showed no significant differences in LPS level, meanwhile statistically significant reduction in body weight was observed in all probiotic groups, LrFBB81 (Δ=-15.7 gram, p=0.040), LrSKG34 (Δ= -20.43 gram, p=0.006), and LrFSMM22 groups (Δ=-18.33 gram, p=0.037).CONCLUSION: The administration of L. rhamnosus could improve FBG, HOMA-IR, and reduce body weight without suppressing the LPS.KEYWORDS: diabetes, probiotic, Lacticaseibacillus rhamnosus, fasting blood glucose, HOMA-IR, lipopolysaccharide, insulin resistance
[Erratum] Oxidative Stress Mediates the Association between Thyroid Dysfunction and Breast Cancer Mahmood, Maira; Mushtaq, Mazhar; Malik, Aamenah; Khurshid, Rukhshan; Aamir, Faiza; Javed, Arham
The Indonesian Biomedical Journal Vol 16, No 6 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The publisher would like to point out that the heading of Table 2, Table 3 and Figure 1 were incorrectly printed. The following headings are correct.Table 2. Thyroid profile of control (n=100) and case (n=288) groups.Table 3. Antioxidative and hormonal profile of control (n=100) and case (n=288) groups.Figure 1. CTSS levels of control (n=100) and case (n=288) groups. A significant difference between groups is indicated by *p<0.01. ---Orginal article: Indones Biomed J. 2023; 15(4): 303-9.
Ultraviolet B (UVB) Radiation Induces Skin Alterations, Emperipolesis and Decreases the Erythroid-to-myeloid Ratio in Rats Farida, Verda; Dewananda, Dion Adiriesta; Sahid, Muhammad Novrizal Abdi; Salasia, Siti Isrina Oktavia
The Indonesian Biomedical Journal Vol 16, No 6 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i6.3227

Abstract

BACKGROUND: Ultraviolet B (UVB) radiation is commonly known to be related to skin inflammation. The inflammation process is orchestrated by many cell types, including immune cells. Changes in bone marrow cellularity can also be an indicator of inflammation. Megakaryocytes, myeloid immune cells progenitor and erythroid progenitor cells, are at high risk of changes upon UVB irradiation. However, there are still limited study observing the change of bone marrow cell population after UVB irradiation. Therefore, this study was conducted to investigate the alteration of the skins, emperipolesis, and the change in erythroid and myeloid cell population in bone marrow after UVB irradiation.METHODS: Ten Wistar rats were divided equally into control and UVB-irradiated group. The skin superficial condition before and after UVB irradiation was observed with a skin analyzer camera. On the 9th day, skin tissues were processed for the observation of general skin structure with hematoxylin-eosin (HE) staining, mast cells infiltration with toluidine blue staining, and collagen fibers with Mallory staining. Bone marrow and peripheral blood samples were collected and proceeded for Giemsa-staining to observe the cell population.RESULTS: Erythema appeared on the skin as marked by orange-red spots. There were hyperkeratosis and pigment accumulations in the skin of UVB-irradiated group. The depletion of collagen-density and hemorrhage were clearly observed in the skin of UVB-irradiated group. There were higher mast cell numbers in the UVB-irradiated skin compared to non-treated skin. The erythroid-to-myeloid ratio in the bone marrow was decreased to around 1.6:11.2 from the normal ratio of 1:4. In addition, emperipolesis was observed in the bone marrow induces by UVB-irradiation.CONCLUSION: These results indicate that UVB-irradiation alters the skin structure, erythroid-to-myeloid ratio, and induced emperipolesis.KEYWORDS: emperipolesis, erythroid, myeloid, skin, UVB radiation
Luteolin Suppresses Endothelial Permeability and Nitric Oxide Scavenging Effects Yoong, Wong Theen; Choong, Shuit Siew; Fauzee, Mohd Sofian Omar; Ahmad, Zuraini; Hakim, Muhammad Nazrul
The Indonesian Biomedical Journal Vol 16, No 5 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i5.3198

Abstract

BACKGROUND: Numerous inflammatory diseases are linked to increased endothelial permeability through the nitric oxide (NO) flux in endothelial cells. Luteolin has in vitro and in vivo anti-inflammatory properties and has been reported to reduce endothelial permeability. However, the exact mechanism/s are yet to be determined. Thus, the present study was conducted to investigate the effects of luteolin in reducing endothelial permeability in vitro using bradykinin (BK) or sodium nitroprusside (SNP) through the NO pathway and the NO radical scavenging property of luteolin.METHODS: Human umbilical vein endothelial cells (HUVECs) in the treatment groups were dosed with luteolin at 5, 10, and 25 µM concentrations and allowed to incubate for one hour prior to induction. The L-NAME or HOE 140 were administered prior to the induction of BK or SNP in HUVECs. The NO radical scavenging test, the nitrite determination assay using L-NAME as antagonist, and the in vitro vascular permeability testing using HOE 140 as antagonist were performed.RESULTS: Endothelial permeability was decreased dose-dependently by 5, 10, and 25 µM luteolin in vitro via lowering NO generation. In comparison to HOE 140, luteolin suppressed the enhanced endothelial permeability more effectively. The suppression was 98.02% by 25 µM luteolin compared to HOE 140 94.05%. It was also discovered that luteolin, when incubated with SNP in a dose-dependent manner, possessed potent NO radical scavenging activities.CONCLUSION: The current data demonstrated luteolin's ability to scavenge NO radicals and significantly decrease endothelial permeability through the NO route. Thus, in complementary medicine, luteolin might be potential to improve endothelial permeability suppressor in reducing inflammation.KEYWORDS: luteolin, endothelial permeability, NO, scavenging property, HUVECs

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