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All Journal Best Journal (Biology Education, Sains and Technology) Indonesian Journal of Health Science Sehat Rakyat: Jurnal Kesehatan Masyarakat Health Research Journal of Indonesia
Putri Mulia
Universitas Bengkulu
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Determination of total alkaloid content in 70% and 96% ethanol extracts of red seaweed (Eucheuma spinosum) obtained by maceration using UV-Vis spect Sindi Arlin Natasya; Dwi Kurnia Putri; Rose Intan Perma Sari; Samwilson Slamet; Putri Mulia; Nurfitriyana Nurfitriyana
Indonesian Journal of Health Science Vol 6 No 3 (2026)
Publisher : PT WIM Solusi Prima

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54957/ijhs.v6i3.2261

Abstract

Red seaweed (Eucheuma spinosum) is one of the marine biological resources containing various secondary metabolites, including alkaloids which possess important biological activities. Differences in ethanol concentration may affect the amount of alkaloid compounds extracted. This study aimed to compare the alkaloid content of 70% ethanol extract and 96% ethanol extract of red seaweed (Eucheuma spinosum) obtained by maceration using the UV-Visible spectrophotometric method. This research was conducted experimentally in the laboratory using the maceration extraction method with 70% and 96% ethanol solvents. Qualitative identification of alkaloids was performed using Mayer, Wagner, and Dragendorff reagents, while quantitative determination was carried out using UV-Visible spectrophotometry with caffeine as the standard at a maximum wavelength of 275 nm. Phytochemical screening results indicated that both 70% and 96% ethanol extracts were positive for alkaloid compounds. The extraction yield of the 70% ethanol extract was 22.945%, whereas the 96% ethanol extract yielded 12.45%. The results of alkaloid determination showed that the 70% ethanol extract contained an average alkaloid level of 5.5 ± 0.22 mg QCE/g, while the 96% ethanol extract contained 5.3 ± 0.10 mg QCE/g. It can be concluded that the 70% ethanol extract produced a slightly higher alkaloid content than the 96% ethanol extract of red seaweed (Eucheuma spinosum).
Hubungan Pengetahuan dengan Sikap Masyarakat pada Penggunaan Antibiotik di Kelurahan Bumi Ayu Kota Bengkulu Defi Suryanti; Oky Hermansyah; Putri Mulia; Tri Danang Kurniawan; Dwi Dominica
Sehat Rakyat: Jurnal Kesehatan Masyarakat Vol. 5 No. 3 (2026): Agustus 2026
Publisher : Yayasan Pendidikan Penelitian Pengabdian Algero

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54259/sehatrakyat.v5i3.7554

Abstract

Irrational use of antibiotics in the community remains a health problem that can increase the risk of antimicrobial resistance, Low levels of public knowledge are suspected to influence the formation of inappropriate attitudes toward antibiotic use. This study aimed to analyze the relationship between the level of knowledge and community attitudes toward antibiotic use in Bumi Ayu Village, Bengkulu City. This study used a quantitative method with an observational analytic design through a cross-sectional approach. The sample consisted of 110 respondents selected using a proportional cluster random sampling technique. Data were collected using questionnaires that had been tested for validity and reliability, with Cronbach’s Alpha values of 0.702 for the knowledge variable and 0.786 for the attitude variable. Data were analyzed using univariate and bivariate analyses with the Chi-Square test. The results showed that most respondents had low knowledge levels, with 50 respondents (45.5%), and poor attitudes, with 50 respondents (45.5%). The bivariate analysis showed a significant relationship between the level of knowledge and community attitudes toward antibiotic use, with a p-value of 0.000 (p < 0.05). Respondents with better knowledge tended to have better attitudes toward antibiotic use. Therefore, continuous health education through health workers and information media is needed to improve public understanding of rational antibiotic use in order to prevent antimicrobial resistance.
Co-Authors : Samwilson Slamet, : Samwilson Asbedta Anggraini Assyifa Ainalaysia Betta Nirwana Defi Suryanti Deni Maryani Dessy Triana Dominica, Dwi Dwi Kurnia Putri Farid, Muchammad Guntur Saputra Hera Olani Jaya Ningsih Ikhsan Ikhsan Maria Eka Patri Y Mauliani Dwi Nathasyah Nabila Aulia Putri Nesa Romanda Sari Nuraisyah Fitri Lesti Nurfitriyana Nurfitriyana Oky Hermansyah Regina Anggun Pitri Rifa Rihadatul Aisy Riska Dea Ananda Risky Hadi Wibowo Rose Intan Perma Sari Septi Wulandari Sindi Arlin Natasya Sipriyadi Suci Rahmawati Sutra Elvyanti Syahbananda Zulyan Palse Tiara Nisrina Fitri Tika Hardini Tri Danang Kurniawan Try Nafilah Nujjiah Vernonia Yora Saki Yona Harianti Putri