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All Journal Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Isamic Communication Journal E-Dimas: Jurnal Pengabdian kepada Masyarakat Alhadharah: Jurnal Ilmu Dakwah Kamaya: Jurnal Ilmu Agama MUHARRIK: JURNAL DAKWAH DAN SOSIAL Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Jurnal Ilmiah Inovasi Mawaizh : Jurnal Dakwah dan Pengembangan Sosial Kemanusiaan Dakwatuna: Jurnal Dakwah dan Komunikasi Islam Jurnal Ilmu Peternakan Terapan TADBIR: Jurnal Manajemen Dakwah FDIK IAIN Padangsidimpuan Jurnal Ilmiah Agribios Stock Peternakan Prosperity: Journal of Society and Empowerment Alhadharah: Jurnal Ilmu Dakwah MEDIOVA: Journal of Islamic Media Studies JAS (Journal of Animal Science) Fastabiq: Jurnal Studi Islam Applied Information Technology and Computer Science (AICOMS) Ulil Albab
Mustofa Hilmi
Program Studi Teknologi Pengolahan Hasil Ternak, Politeknik Negeri Banyuwangi
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Social Sciences Veterinary Other

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Journal : Stock Peternakan

 1 
TECHNIQUES OF STORAGE STAPHYLOCOCCUS AUREUS ON CELL VIABILITY AS A REFERENCE FOR ISOLATE PRESERVATION IN MICROBIOLOGY LABORATORIES Yannuarsita, Deni; Sasmito, Moch Shandy; Riyadi, Eko Slamet; Hilmi, Mustofa
STOCK Peternakan Vol 7, No 2 (2025): Stock Peternakan
Publisher : Universitas Muara Bungo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36355/sptr.v7i2.1870

Abstract

Preservation of isolates is carried out as an effort to collect and conserve microbial germplasm and to ensure its availability for future research and applications. The purpose of this research to evaluated the effectivity of temperature (5ºC and -4ºC) during 84 days to prevent viability of staphylococcus aureus, which can be used as a model inactivation kinetic and recommendation determine in the microbiology laboratorium. The research was conducted at the Microbiology Laboratory of the Agricultural Department, Banyuwangi State Polytechnic, from June to September 2025. The temperature are 5°C and –4°C, and the period are 0, 14, 28, 42, 56, 70 and 84 days. All test isolates were suspended in Tryptone Soy Broth (TSB) and supplemented with 15 % glycerol. Analysis of variance revealed a highly significant interaction (P 0.001) between temperature and storage period on Staphylococcus aureus viability. At 5 °C, colony bacteria decrease from days 14 until days 80 is 0.95 log CFU/mL. At –4 °C the population of colony bacteria dropped by approximately 1.7 log CFU/mL. The study concludes that, Short-term preservation can be achieved at 5 °C, with sub-culturing recommended at weeks 4–6, Medium-term preservation at –4 °C allows sub-culturing after month 6, Long-term preservation at –4 °C is feasible, with sub-culturing possible after month 12. 
Co-Authors Abdul Rozaq Ahmad Anas Airul Alrizal Rofiqi Akhmad Afandi Alfandi, M Alifa Nur Fitri Amelia Rahmi Anasom, Anasom Anis U. Prastujati Anis Usfah Prastujati Anis Usfah Prastujati Asmaul Khusna Butsainah, Innas Salma Chairullah, Dimas Diansah, Danang Putra Diyana, Dinunah Dyah Triasih Farida Rachmawati, Farida Fathonah, Aida Fatikhaturrohmah, Salsabilla Fitri Fitri Hartanto, Abdi Hatta Abdul Malik Herman Yuliandoko, Herman Hidayaturrohman Hidayaturrohman Islamiyah, Niswatul Kasmuri Kasmuri, Kasmuri Khirzin, Muhammad Habbib Khusna, Asmaul Kurniawan Prasetyo Kusuma, Dedy Hidayat Lusi, Nuraini M Habbib Khirzin Maya Rini Handayani Muhajarah, Kurnia Muhammad Abdul Wahid Muhammad Habbib Khirzin Musyafak, Najahan Ninik Sri Rahayu Wilujeng Nur Fitri, Alifa Nusrotin Mawardi Prastujati, A. U. Prisdayanti, Sefdha Riyadi, Eko Slamet Riza Rahayu Rofiqi, Airul Alrizal Sasmito, Moch Shandy Silvia Riskha Fabriar Soleha, Dena Walda Triasih, Dyah Vina Darissurayya, Vina Yannuarista, Deni Yannuarsita, Deni Yushandy Fajar Thriesnanda Yuyun Affandi