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All Journal Indonesian Journal of Tropical and Infectious Disease Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Jurnal Kesehatan Manarang GUIDENA: Jurnal Ilmu Pendidikan, Psikologi, Bimbingan dan Konseling Care : Jurnal Ilmiah Ilmu Kesehatan INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Jurnal Layanan Masyarakat (Journal of Public Service) CURRENT INTERNAL MEDICINE RESEARCH AND PRACTICE SURABAYA JOURNAL Jurnal Inovasi, Pemberdayaan dan Pengabdian Masyarakat Journal of Clinical Microbiology and Infectious Diseases
Wardhani, Puspa
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Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Library & Information Science Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Journal : INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY

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Characterization of Aedes sp. larvae: Detection of dengue, Japanese encephalitis and West Nile virus with knockdown resistance mutations in Mulyorejo, Surabaya, Indonesia Prasasti, Adelia Gita; Sucipto, Teguh Hari; Kurniawan, Muhammad Ridho Hafid; Fauziyah, Shifa; Riris, Anak Agung Istri Dalem Cinthya; Djurumana, Yohana; Hapsari, Nafisah Nurul; Farihah, Neni Isna; Wijayanti, Alvia Rachma; Kusumawati, Nita; Hariyono, Hariyono; Wardhani, Puspa
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 8 No 1 (2026): Integration of Molecular Approaches in Addressing Drug Resistance and Changing Gl
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v8i1.6654

Abstract

Indonesia recorded the highest dengue mortality rate in Asia in 2022, with 45,387 cases and 432 deaths.  This study aimed to investigate the coexistence of arboviruses and insecticide resistance mechanisms in Aedes vectors mosquitos’ populations in Mulyorejo District, Surabaya, during the early monsoon season of 2021. A cross-sectional survey was conducted in January 2021, and Aedes larvae were collected and reared to adulthood under laboratory conditions. A total of 309 Aedes larvae (comprising 65% Aedes aegypti and 35% Aedes albopictus) were collected from 36 breeding sites, identified, and georeferenced using QGIS software. RNA extraction and RT-PCR assays were performed to detect Dengue virus (DENV), Japanese encephalitis virus (JEV), West Nile virus (WNV), and knockdown resistance (KDR) mutations. Results showed seven samples positive for DENV-2 and seven for JEV, with two samples (codes 9 and 12) exhibiting co-infection with both viruses, while no WNV was detected. The detection of JEV in Aedes larvae is a notable finding, suggesting a potential secondary vector role for these species in the study area, as Culex mosquitoes are the primary vectors of JEV. Mutations at codons 1016 (V1016G) and 1534 (F1534C), associated with pyrethroid and DDT resistance, were detected in eight and seven samples, respectively. These findings confirm the circulation of DENV-2 and JEV in local Aedes populations, highlighting the presence of KDR mutations that confer insecticide resistance. The results underscore the urgent need for integrated vector management in Surabaya, combining arbovirus surveillance, insecticide resistance monitoring, and community-based source reduction to mitigate arboviral disease transmission.
Association between postnatal maternal serology and congenital cytomegalovirus infection in neonates with jaundice Wardhani, Puspa; Pamungkas, Joko; Husada, Dominicus; Ernawati, Ernawati; Purnami, Nyilo; Fitriah, Munawaroh; Prasetyani, Reni
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 8 No 1 (2026): Integration of Molecular Approaches in Addressing Drug Resistance and Changing Gl
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v8i1.7737

Abstract

Congenital cytomegalovirus (CMV) infection is one of the most prevalent infections at birth and can lead to severe health complications in neonates. Timely detection is crucial. However, polymerase chain reaction (PCR) testing is often unavailable in numerous healthcare settings. In such cases, maternal CMV antibody testing may assist in identifying at-risk infants. In the development of diagnostic methods to date, there is no single method for detecting CMV infection in pregnant women at all stages of pregnancy. This study examined the relationship between maternal CMV serology after delivery and congenital CMV infection in newborns presenting with symptoms including jaundice, prematurity, or low birth weight. Urine samples from neonates within the first 21 days of life were analyzed for CMV DNA via PCR, while maternal IgG and IgM antibodies were assessed within three weeks after delivery. A total of 87 neonates were examined, with 28 (32.2%) testing positive for CMV DNA. Neonatal jaundice was significantly associated with CMV infection, whereas prematurity and low birth weight were not. Maternal CMV IgM positivity was significantly correlated with neonatal CMV PCR positivity, while maternal IgG did not exhibit a significant relationship. In conclusion, maternal CMV IgM positivity post-delivery may assist in identifying newborns requiring confirmatory PCR testing, particularly in healthcare environments with constrained diagnostic resources.
Co-Authors Alelo, Richardo Reynaldi Sakka Almira, Naufalia Ghina Aryati Aryati Aulia, Fauqa Arinil Azza, Irine Irchamillah Bintoro, Siprianus Ugroseno Yudho Djurumana, Yohana Dominicus Husada Eko Budi Koendhori, Eko Budi Ernawati Ernawati Fahimah Martak Farihah, Neni Isna Fitriah, Munawaroh Hapsari, Nafisah Nurul Hariyono Hariyono Harsasi Setyawati Hasan, Helmia Hermina Novida, Hermina ida, maulidah Ilham Harlan Amarullah Imam Susilo Joko Pamungkas Kris Cahyo Mulyatno, Kris Cahyo Kurniawan, Muhammad Ridho Hafid Kusdiana, Asep Maharani, Anisa Masanori Kameoka, Masanori Nita Kusumawati Nyilo Purnami Prasasti, Adelia Gita Prasetyani, Reni Putri, Anandia Nafisah Rahardjo, Albertus Reiner Ratri, Maya Masita RINI RINI Riris, Anak Agung Istri Dalem Cinthya Roelanda, Alvi Romadhon, Pradana Zaky Septiarini, Namira Amelia Setya Wijoyo, Dhia Lintang Shifa Fauziyah Siti Churrotin, Siti Siti Nur Asiyah Soegeng Soegijanto Sri Subekti Sri Sumarsih Suhendra Teguh Hari Sucipto, Teguh Hari Tomohiro Kotaki, Tomohiro Triyani, Irma Ueda, Shuhai Ummi Maimunah Virdiyanti, Ruri Wibrianto, Aswandi Wijayanti, Alvia Rachma Yetti Hernaningsih Yulia Nadar Indrasari