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All Journal Bioma : Berkala Ilmiah Biologi Biotika: Jurnal Ilmiah Biologi El-Hayah : Jurnal Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Pengabdian Kepada Masyarakat Sakai Sambayan Jurnal Penelitian Sains Jurnal Biologi Tropis Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal Biogenerasi Journal of Community Service and Empowerment Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun Jurnal Pengabdian Dharma Wacana BIOVALENTIA: Biological Research Journal Jurnal Mitrawarga JURNAL BIOLOGI PAPUA The International Journal of Tropical Veterinary and Biomedical Research BIO-SAINS | Jurnal Ilmiah Biologi
Priyambodo Priyambodo
Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Lampung
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Journal : BIOVALENTIA: Biological Research Journal

 1 
THE ESSENTIAL CONTRIBUTION OF CAPTIVE SUMATRAN ELEPHANT IN ELEPHANT TRAINING CENTER, WAY KAMBAS NATIONAL PARK FOR WILDLIFE GENETICS CONSERVATION Rustiati, Elly Lestari; Priyambodo, Priyambodo; Yulianti, Yanti; Srihanto, Eko Agus; Pratiwi, Dian Neli; Virnarenata, Elsa; Novianasari, Tika; Krismuniarti, Elisabeth Devi; Saswiyanti, Enny
BIOVALENTIA: Biological Research Journal Vol. 6 No. 1 (2020)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1061.173 KB) | DOI: 10.24233/BIOV.6.1.2020.173

Abstract

Way Kambas National Park (WKNP) is home of five protected big mammals including sumatran elephants.  It shares its border with 22 of 37 villages surrounding the national park.  Understanding their existence in the wild is a priority, and  wildlife genetics is a crucially needed. Besides poaching and habitat fragmentation, wildlife-human conflict is one big issue.  Elephant Training Center (ETC) in WKNP is built for semi in-situ conservation effort on captive sumatran elephants that mainly have conflict histories with local people.  Participative observation and bio-molecular analysis were conducted to learn the importance of captive Sumatran elephant for conservation effort.  Through captive sumatran elephants, database and applicable methods are expected to be developed supporting the conservation of their population in the wild.  Participative observation and molecular identification was carried on captive sumatran elephants in ETC, WKNP under multiple year Terapan grant of Ministry of Research and Technology Higher Education, Indonesia. Gene sequence and cytological analyses showed that the captive sumatran elephants are closely related and tend to be domesticated.  Translocation among ETC to avoid inbreeding, and maintaining the captive sumatran elephant as natural as possible are highly recommended. Developing genetic database can be a reference for both captive and wild sumatran elephants.
IDENTIFICATION AND CHARACTERISATION OF COI GENE IN FEMALE SUMATRAN ELEPHANT (Elephas maximus sumatranus) IN ELEPHANT TRAINING CENTRE, WAY KAMBAS NATIONAL PARK Virnarenata, Elsa; Rustiati, Elly Lestari; Priyambodo, Priyambodo; Srihanto, Eko Agus; Pratiwi, Dian Neli
BIOVALENTIA: Biological Research Journal Vol. 7 No. 1 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (716.49 KB) | DOI: 10.24233/biov.7.1.2021.175

Abstract

Sumatran elephant is a subspecies of endemic Asian elephants on the island of Sumatra and is included in the Red List of the International Union for Conservation of Nature (IUCN) with critically endangered status. The building of the Elephant Training Centre (ETC) in Way Kambas National Park (WKNP) is one of the conservation efforts of Sumatran elephants. Small and closed population size lead to an increased risk of inbreeding that triggers reduction in genetic variation and viability and increases the risk of extinction. The phylogenetic pattern of Sumatran elephants in Indonesia has shown a low population genetic diversity. Genetic diversity information is indispensable to support the direction of decision making in Sumatran elephant conservation policy. The DNA isolation of Sumatran elephants in ETC, WKNP has performed as a first step to trace its genetic variation. The advanced step of DNA isolation is the use of cytochrome oxidase subunit I (COI) gene for identification of genetic characteristics in Sumatran elephants. The COI gene is one of the genes on the mitochondrial genome and in molecular studies it is used as a genetic marker to study genetic characteristics between species and individuals. Identification and characterisation are done by sequencing process and data analysis in the form of electroforegram using Molecular Evolution Genetics Analysis (MEGA) software version 6.0. to see the genetic diversity of the female Sumatran elephant population in ETC, WKNP. Based on the results of the analysis it is indicated that the genetic distance of 24 individual female Sumatran elephant from PLG, TNWK is 0.000 with a homology value of 100%, strengthened by the construction of phylogenetic tree. The absence of genetic distance indicates a close genetic relationship, so it can be concluded all individual female Sumatran elephants in the PLG, TNWK is derived from one population group.
PRIMER OPTIMIZATION AS GENETIC MARKER PRIMER BASED ON CYTOCHROME B GENE ON SUMATRAN ELEPHANT (Elephas maximus sumatranus) NON-INVASIVE SAMPLES Eko Agus Srihanto; Elly Lestari Rustiati; Priyambodo Priyambodo; Dian Neli Pratiwi; Alvin Wiwiet Susanto; Diah Esti Anggraini; Enny Saswiyanti
BIOVALENTIA: Biological Research Journal Vol. 8 No. 1 (2022)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (773.02 KB) | DOI: 10.24233/biov.8.1.2022.216

Abstract

Sumatran elephant is declared as critically endangered by IUCN since 2011. In supporting conservation efforts on sumatran elephant in its natural habitat, molecular data collection of their population needs to be done. Genetic source material is mostly obtained in its natural habitat is its dung/feces. Study on cytochrome B gene as genetic marker from sumatran elephant fecal samples can be done to get additional genetic data as a form of conservation effort. Primer optimization as genetic marker based on cytochrome B gene has been carried out as an early study on sumatran elephant genetics.  Primer optimization program based on cytochrome B genetic marker from sumatran elephant fecal samples was under Higher Education Applied Research funded by The Ministry of Research, Technology, and Higher Education. DNA was isolated from fecal samples of captive sumatran elephants in Elephant Training Center, Way Kambas National Park. Primer optimization was done via amplification by increasing certain temperature. Specificity test was done to ensure that the primer only recognized sumatran elephant DNA. The best result was obtained with the annealing temperature of 52oC
Co-Authors Agustin, Meli Agustrina, Rochmah Alvin Wiwiet Susanto Alvin Wiwiet Susanto Amaliya Sabana Ani A. Yani Ani Andri Yani Ani Andri Yani Aprilia Eka Putri Arsan, Zulfi Ayuni, Ubaid Jan Bambang Irawan Bondan Pergola Chicka Refina P. Putri Danisworo Zulkarnain DEDI CANDRA Diah E. Anggraini Diah Esti Anggraini Dian Neli Pratiwi Dian Neli Pratiwi Dian Neli Pratiwi Eko A. Srihanto Eko Agus Srihanto Eko Agus Srihanto Eko Agus Srihanto, Eko Agus Elizabeth D. Krismuniarti Elly L. Rustiati Elly L. Rustiati Elly L. Rustiati Elly Lestari Rusitati Elly Lestari Rustiati Emantis Rosa Enny Saswiyanti Enny Saswiyanti Enny Saswiyanti Erdi Suroso Eti Ernawiati Eti Ernawiati Eva Yunita Firwantoni Firwantoni Ganis Mustikawati Gina Dania Pratami, Gina Dania Giyono Giyono Giyono Giyono I Gede Swibawa Ibransyah, Rasyid Indah A. Lestari Indah Ayu Lestari Indah Ayu Lestari Intan Diani Fardinatri Irwan Nasution Jani Master Jani Master, Jani Junaidi Junaidi Krismuniarti, Elisabeth Devi Kurniawati, Yeyen Lestari, Septi Wahyu Lili Chrisnawati Liza Angeliya Lousanja D. Sa'uddah Lousanja Dira Sa’uddah Lousanja Dira Sa’uddah Lu'lu' Kholidah Fauziah Lumbanraja, Favorisen R Mahfud Sidik Mahfud Sidik Mahfud Sidik Martha Lulus Lande Masniar Vina Kesti Melya Riniarti Mustikawati, Ganis Nindy Permatasari Novianasari, Tika Nugraha, M. Aqwam Nuning Nurcahyani Patrice Kevin Marcus Pertiwi, Vindo R. Pratiwi, Dian N. Pratiwi, Dian Neli Putri Ayu Febrina Putri, Chicka R.P. Resti Puspa Kartika Sari Rochmah Agustrina Rochmah Agustrina Rodliyah, Aini Robby Romaya Wulan Suciningtyas Rustiati, Elly L. Sandra, Shifa Saswiyanti, Enny Sekar Pratiwi Siti Asiyah Srihanto, Eko A. Sukatmoko Sukatmoko Sukatmoko, Sukatmoko Susanto, Alvin Wiwiet Susanto, Gregorius Nugroho Thesalonika, Natasya Tristiyanto Tristiyanto Tugiyono Tugiyono Tugiyono Tugiyono Vindo R. Pertiwi Virnarenata, Elsa Warsono Warsono Winarno Winarno Winda Rahmawati Yanti Yulianti Yeyen Kurniawati Yuni Tinasari Zulfi Arsan Zulkarnain, Danisworo