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EFEKTIFITAS PERENDAMAN TELUR DALAM LARUTAN HORMON TIROKSIN DENGAN DOSIS BERBEDA TERHADAP DAYA TETAS, PERTUMBUHAN DAN KELANGSUNGAN HIDUP LARVA IKAN NILEM (Osteochilus hasseltii) Taufik Budhi Pramono; Yola Vebiola; Sri Marnani; Marhaendro Santoso; Kasprijo .
Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Vol 9, No 1 (2021): Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan
Publisher : UNIVERSITAS MUHAMMADIYAH PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (655.704 KB) | DOI: 10.29406/jr.v9i1.2438

Abstract

High mortality in the larvae stage was a major problem in fish hatchery efforts. Efforts that can be done through the hormonal stimulation with the utilization of thyroxine hormone. The purposes of this research was to determine the effect of immersing eggs in a solvent of thyroxine hormone at different doses on hatchability, growth and survival nilem fish larvae (Osteochilus hasseltii). The research method use a complete randomized design (RAL). Treatments in this research were A (control), B (0.05 mg / L), C (0.10 mg / L), D (0.15 mg / L). The research variables observed were hatchery, an absolute long-term growth, relative long-term growth and the survival. The results of this research showed that the soaking of eggs in thyroxine hormone at different doses had no significant effected on hatchability (P> 0.05) but had a significant effected on growth, and survival of nilem fish larvae (P
Performa Pertumbuhan dan Ekspresi Gen Hormon Pertumbuhan Sidat (Anguilla bicolor bicolor) Stadia Elver Yang diberi Pakan Mengandung Kromium Pikolinat Dewi Nugrayani; Anandita Ekasanti; Hamdan Syakuri; Emyliana Listiowati; Petrus Hary Tjahja Soedibya; Sri Marnani; Marhaendro Santoso; Rudy Wijaya; Purnama Sukardi; Kasprijo Kasprijo
Jurnal Akuakultur Sungai dan Danau Vol 8, No 1 (2023): April
Publisher : Universitas Batangahari Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33087/akuakultur.v8i1.152

Abstract

The administration of chromium picolinate (Cr-Pic) is expected to positively influence the growth of eel (Anguilla bicolor) and the expression of the growth related gene. The purpose of this research was to study the effect of chromium picolinate (Cr-Pic) on the growth performance and expression of growth hormone encoding gene of eel (Anguilla bicolor), a species of fish that is in the process of domestication. The experiment consisted of four treatments: without Cr-Pic (P0), with Cr-Pic of 1 mg / kg of feed (P1), 2 mg / kg of feed (P2), and 3 mg / kg of feed (P3). Eel growth performance was analyzed based on absolute and daily growth rates. Observation of the level of growth hormone (GH) gene expression was performed using real-time RT-PCR method. The results showed that administration of Cr-Pic did not significantly increase eel growth rate. The expression level of growth hormone genes in eel fed 2 and 3 mg Cr-Pic / kg was higher than the expression level of this gene in eel fed without Cr-Pic. The results showed that the level of growth hormone expression did not appear to be related to growth rate of the eel. 
Blood Profile of Tilapia (Oreochromis niloticus) Strains Sultana, Nirwana and Larasati against Aeromonas hydrophyla infection Rima Oktavia Kusuma; Muhammad Sulaiman Dadiono; Kasprijo Kasprijo; Muhammad Nurhafid
Jurnal Agroqua: Media Informasi Agronomi dan Budidaya Perairan Vol 20 No 1 (2022): Jurnal Agroqua
Publisher : University of Prof. Dr. Hazairin, SH

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32663/ja.v20i1.2303

Abstract

Aeromonas hydrophyla is a gram negative bacteria, which often infects tilapia and causes internal damage to the liver, spleen and kidneys. Tilapia Sultana, Nirwana and Larasati are superior strains that have fast growth and are resistant to disease. This study was conducted to observe and compare the physiological response of the three strains to Aeromonas hydrophyla infection through blood profiles. The blood profile can describe the health status of the fish. The parameters of the blood profile observed were the number of erythrocytes, hemoglobin, hematocrit, blood glucose, lymphocytes and monocytes. The results showed that the erythrocytes, monocytes and polymorphonuclear counts of the three strains were significantly different, while for hemoglobin, hematocrit, glucose, and lymphocytes were not significantly different.
Teknik Budidaya Ikan Guppy HB Gold (Poecilia reticulata) Skala Rumah Tangga dengan Substrat yang Berbeda Dadiono, Muh. Sulaiman; Kasprijo, Kasprijo; Wijaya, Rudy; Santoso, Marhaendro; Ma'haudis, Ahmad; Yoka, Ananda; Putri, Nadiya Marshandi; faidlurrahman, Faidlurrahman; Umaro, Javid Azka
MAIYAH Vol 2 No 1 (2023): Maiyah : Vol.2 No.1 Maret 2023
Publisher : Fakultas Perikanan dan Ilmu Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (892.699 KB) | DOI: 10.20884/1.maiyah.2023.2.1.8190

Abstract

Ikan guppy HB Gold (Poecilia reticulata) merupakan salah satu strain guppy yang mudah untuk dibudidayakan serta memiliki permintaan yang tinggi. Tujuan penelitian ini adalah untuk menambah wawasan tentang cara budidaya ikan guppy HB Gold (Poecilia reticulata) skala rumah tangga dengan substrat dasar yang berbeda, dari penelitian ini diharapkan dapat diaplikasikan oleh masyarakat umum untuk memulai usaha perikanan dengan modal kecil. Metode yang digunakan menggunakan Rancangan Acak Lengkap (RAL) dengan 3 perlakuan dan 2 ulangan. Hasil penelitian dianalisis secara deskriptif serta di bandingkan dengan literatur terdahulu. Hasil penelitian menunjukkan bahwa semua perlakuan mampu menghasilkan benih ikan dan tumbuh dengan baik. Dimana P1A menetaskan 27 larva; P2A 0 larva; P3A 26 larva, sedangkan pada P1B dan P2B masing-masing 4 larva dan P3B sebanyak 21 larva. Parameter kualitas air diperoleh suhu akuarium pemijahan dan pembesaran antara 25-26°C, dan pH antarar 6,5-8. Maka dari itu dapat diketahui bahwa budidaya ikan guppy skala rumah tangga dapat diterapkan.
The Effect of Using an Autofeeder on Vannamei Shrimp (Litopenaeus vannamei) Growth in Intensive Ponds at Cv. Cemara Sewu Sumber Rejeki, Cilacap -, Andreas Eka Saputra; kasprijo, Kasprijo; Sukardi, Purnama
Journal Of Artha Biological Engineering Vol. 2 No. 1 (2024)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/6jvxk275

Abstract

The use of automatic feeding equipment can increase the productivity of vannamei shrimp cultivation and harvest yields. This research aimed to determine which of the three feeding methods (manual, 120° autofeeder, and 360° autofeeder) produces greater growth and production results for vannamei shrimp. The shrimp used came from Sura Tani Pemuka which were stocked from 23-25 July 2022 at PL (post larval) age 11. When using a 360° autofeeder with a total area of 5,880 m2 and a density of around 169 individuals/m2. When using a 120° autofeeder with a total area of 2,840 m2 and a density of around 166 fish/m2. The manual method is used with a total area of 2,500 m2 and a density of around 187 individuals/m2. The research method was carried out by means of descriptive observation and analysis. Data collection was carried out during shrimp sampling activities, checking water quality, and harvesting. The research results showed that the use of a 360° autofeeder, 120° autofeeder, and manual resulted in sequential growth performance including: survival rates of 39.37%, 51.47%, and 32.47%; average weight growth of 21.53 gr/head, 17.47 gr/head, and 10.12 gr/head; daily weight growth of 0.31 gr/day, 0.25 gr/day and 0.16 gr/day; and, feed conversion ratios were 1.84, 1.73 and 2.23. The harvest results achieved using the 360° autofeeder, 120° autofeeder, and manual method reached 4558.32 kg, 3670.18 kg, and 1294.965 kg, respectively.
EFFECT OF ASTAXANTHIN ON THE COLOR INTENSITY OF GUPPY FISH (Poecilia reticulata) soedibya, petrus; Hawari , Abidzar; Kasprijo, Kasprijo; Marnani, Sri; Muslih, Muslih; Fitriadi, Ren
Journal Of Artha Biological Engineering Vol. 2 No. 1 (2024)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/42gtk346

Abstract

Guppy fish is one type of ornamental fish which is in great demand due to its interesting color variations. Color of ornamental fish will be brighter if the feed contains color pigments. The study aims to determine the effect of Astaxanthin supplement in pellets on the color brightness of guppies and to find the best dose of Astaxanthin supplement in pellets on the color brightness of guppies. The method used in the study was complete randomized design (CRD) consisting of five treatments (Astaxanthin supplements of 0%, 2%, 3%, 4% and 5%) and three replications. The experimental fish were male guppy fish Strain HB Red ± 3 cm length with a density of 3 fish/aquarium. Feeding was done twice a day and siphoning was done every afternoon. The parameters observed were Chroma value, survival rate and water quality. The results show that the supplement of Astaxanthin in pellets successfully increase the brightness of guppies and the best dose of Astaxanthin supplement in pellets was 5% with an increase in Chroma value of 4.82%. The survival rate obtained was 100%. Water quality includes an average temperature of 26-27 ℃ and pH of 6-7 and dissolved oxygen (DO) was 6.95-7.93 ppm
Growth of Tilapia (Oreochromis niloticus) Juvenile strain Nirwana with Different Natural Feeding kusuma, rima oktavia; Sulaiman Dadiono, Muh.; Kasprijo, Kasprijo; Fitriadi, Ren; Tere Yeru, Thomas
Journal Of Artha Biological Engineering Vol. 2 No. 1 (2024)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/3bbmwy23

Abstract

The high cost of commercial feed in Nirwana tilapia aquaculture leads to elevated production costs and reduced profits. As a solution, the provision of natural feed is proposed as an alternative to reduce production expenses. This study aims to compare the growth of Nirwana tilapia fed with three different natural feeds: Tubifex sp., Chironomus sp. larvae, and maggots. The experiment was conducted using a completely randomized design with three replicates for each treatment. The feeding rate was 3% of the fish’s body weight, administered three times a day. The parameters measured included absolute biomass, absolute length gain, daily growth rate (Specific Growth Rate, SGR), survival rate (SR), and water quality during the 30-day rearing period. The results showed that feeding Nirwana tilapia with Tubifex significantly increased growth compared to the control group (commercial pellets), Chironomus larvae, and maggots. The absolute biomass from Tubifex feeding was 5.23 ± 0.89 g, absolute length was 4.2 ± 7.8 cm, SGR was 1.52 ± 0.33%, and SR was 90%. The next highest growth was seen in Chironomus larvae, followed by maggots, with the control yielding the lowest results.
MONITORING THE ABUNDANCE OF Vibrio sp. BACTERIA IN THE WATER OF VANAME SHRIMP (Littopanaeus vannamei) BREEDING POND AT JEPARA BRACKISH WATER AQUACULTURE CENTER Trianingtyas, Laela; Ufianah, Ufianah; Ratnaningsih, Navita Desy; Kasprijo, Kasprijo; Riady, Reza Muhammad; Rizkiyanti, Ita; Zainuddin, Muhammad
Journal Of Artha Biological Engineering Vol. 2 No. 2 (2024)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/gq6rj890

Abstract

The presence of vibrio bacteria in the breeding pond is one of the important factors that affect the activities of vaname shrimp breeding. Vibrio sp. bacteria in waters can cause losses if the number is above the threshold that should be. The high abundance of Vibrio sp. bacteria in the cultivation environment is an indicator that shows the potential for disease that can reduce the growth rate and survival of shrimp. The purpose of the study was to determine the abundance and dominance of vibrio bacteria in vaname shrimp breeding ponds using the TPC (Total Plate Count) calculation technique. The methods used include bacterial sampling, preparation of tools and materials, making bacterial culture media (NA and TCBS), dilution, bacterial inoculation, and calculating the number of bacterial colonies. The results of the calculation of bacterial abundance showed that there were 9 breeding ponds with different abundances. The highest abundance was found in pond BV1 with a total bacterial value of 9.6 x 105 CFU/ml. Meanwhile, the highest vibrio dominance was found in pond BV4 with a percentage of 43.69%.
Screening of proteolytic bacteria from the intestines of Tilapia Fish (Oreochromis niloticus) in the mina padi area of ​​Panembangan Village, Cilongok. Syifa Febrianti, Naily; Kasprijo, Kasprijo; Zainuddin, Muhammad; Muhammad Riady, Reza
Journal Of Artha Biological Engineering Vol. 3 No. 1 (2025)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/pm6jjq40

Abstract

Tilapia is a commodity cultivated in the rice-fish farming area of Panembangan, Banyumas. Information related to the presence of potential bacteria in the intestines of fish cultivated in rice-fish areas is still low, especially proteolytic bacteria. The purpose of this study was to determine the proportion and activity index of proteolytic bacteria in the intestines of fish. This study used an observation method with a purposive sampling technique. Proteolytic bacteria were isolated from the intestines of tilapia in blocks A and E. The bacteria that were successfully isolated were grown on 2% skim milk agar media with an incubation time of 48 hours and observed at 24-hour intervals. Based on the results of the study, the proportion of proteolytic bacteria in the intestines of tilapia from both blocks was 28% in block A and 44% in block E, respectively. The proteolytic activity index of both blocks had an average of 0.48 for block A and 0.75 for block E. The highest proteolytic activity index was from block A, namely 0.83, and in block E 3.0. Meanwhile, the smallest proteolytic index is 0.17 block E and 0.22 block A.
GROWTH PERFORMANCE AND INTESTINAL Aeromonas spp. OF BONYLIP BARB (Osteochilus vittatus) AFTER RECEIVING DIET CONTAINING SALT AND HERBAL CONCOCTION SUPPLEMENTATION Syakuri, Hamdan; Soedibya, Petrus Hary Tjahja; Marnani, Sri; Ekasanti, Anandita; Kasprijo, Kasprijo; Kusuma, Rima Oktavia; Wijaya, Rudy; Nugrayani, Dewi; Listiowati, Emyliana; Palupi, Mustika
Indonesian Aquaculture Journal Vol 19, No 2 (2024): (December, 2024)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.19.2.2024.111-122

Abstract

Salt and herbal supplementations in fish feed are expected to promote aquaculture productivity, including for slow-growing fish such as Bonylip barb (Osteochilus vittatus). The objective of this study was to evaluate the growth performance and occurrence of Aeromonas spp. in the intestine of Bonylip barb fed a diet supplemented with salt and herbs. This experiment was conducted using a completely randomized design with four treatments: 1) control, 2) salt 3% supplementation, 3) herbal supplementation, and 4) Salt 3% and herbal supplementation. Growth performance evaluation included weight gain, relative growth rate, and specific growth rate after 30 and 60 days of rearing.  Bacterial samples were isolated on trypticase soy agar (TSA) and glutamate starch phenol red agar (GSP) media. Yellow colonies on GSP were counted and confirmed as Aeromonas spp. via molecular identification based on 16S rDNA sequence. Results showed that herbal supplementation, either alone or in combination with 3% salt, insignificantly increased the fish growth performance in the short-term (30 days), but tended to decrease it over the long-term (60 days). Salt supplementation consistently decreased fish growth performance but improved fish survival rates. Aeromonas isolates comprised more than 60 % of the culturable intestinal bacteria in all treatments. Furthermore, herbal supplementation appeared to increase the percentage of Aeromonas spp. in the intestine, with identified species including Aeromonas veronii, Aeromonas caviae, and Aeromonas jandaei.