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PENAPISAN BAKTERI SELULOLITIK PADA SALURAN PENCERNAAN IKAN KERAPU CANTANG YANG DIBUDIDAYAKAN DI DESA BABAKAN, KECAMATAN PANGANDARAN, KABUPATEN PANGANDARAN Syukri, Ningam; ., Kasprijo; Tjahja, P.Hary; Syakuri, Hamdan; Listiowati, Emyliana
Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Vol 9 No 2 (2021): Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan
Publisher : UNIVERSITAS MUHAMMADIYAH PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (528.946 KB) | DOI: 10.29406/jr.v9i2.3000

Abstract

Ikan Kerapu Cantang merupakan hasil pesilangan dari ikan Kerapu Macan betina dan ikan Kerapu Kertang jantan. Ikan Kerapu Cantang merupakan salah satu komoditas perikanan budidaya yang memiliki nilai ekonomis tinggi. Penelitian ini bertujuan untuk mengetahui keberadaan bakteri selulolitik serta aktivitas bakteri selulolitik pada saluran pencernaan ikan Kerapu Cantang. Metode yang digunakan pada penelitian ini adalah metode observasi dengan pengambilan sampel secara purpose random sampling. Variabel utama yang diamati pada penelitian ini yaitu mengamati proporsi bakteri selulolitik dan aktivitas bakteri selulolitik pada saluran pencernaan ikan Kerapu Cantang. Untuk variabel pendukung pada penilitian ini yaitu kelimpahan bakteri, uji gram KOH, proporsi Bacillus Serta pewarnaan gram. Keberadaan bakteri Selulolitik pada saluran pencernaan ikan Kerapu Cantang pada penelitian ini ditunjukkan dengan adanya zona bening di sekeliling koloni bakteri yang ditumbuhkan pada media spesifik ( CMC 1%). Hasil penelitian ini untuk proporsi keberadaan bakteri selulolitik pada bagian anterior 49,33% middle 38,66% dan posterior 28%. Aktivitas bakteri selulolitik dari penelitian ini menunjukan hasil dengan indeks hidrolisis pada bagian anterior sebesar 0,14 – 1,4, midddle 0,1 – 3,3 dan posterior 0,2 – 1,6.
Effect of Additional Dried Tubifex sp. in Commercial Feed Against Color Intensity of Guppy (Poecilia reticulata) Nurjihan, Haniah Istiqomah; Kasprijo, Kasprijo; Muslih, Muslih; Soedibya, Petrus Hary Tjahja; Fitriadi, Ren; Esa, Yuzine
Journal of Aquaculture and Fish Health Vol. 13 No. 2 (2024): JAFH Vol. 13 No. 2 June 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i2.39061

Abstract

Guppy fish (Poecilia reticulata) is a type of ornamental fish that is in great demand, because of its small size and beautiful color. The color of ornamental fish will generally fade, due to a lack of carotenoids in their feed. The purpose of this study was to determine the effect of giving Tubifex sp. in commercial feed to increase the color intensity of guppy fish and the best dose of Tubifex sp. The test fish used were male guppy fish strain HB Red. This study used a completely randomized design (CRD) method with five treatments and three replications, that is commercial feed with the addition of Tubifex sp. with doses of 0% (P1), 2% (P2), 4% (P3), 6% (P4), and 8% (P5). Parameters observed were color intensity (chroma value), survival, and water quality. Addition of Tubifex sp. in commercial feed gave the effect with the highest yield on P5 at a dose of 8%, with an increase in color intensity (chroma value) of 4,21±0,25d. At P1 it gave an increase of 1,19±0,02a, P2 was 1,34±0,04a, P3 was 1,81±0,21b, and P4 was 2,88±0,18c. So, the best treatment is P5 (8%). Survival showed the results were not significantly different, that is 100%. Water quality is included in the tolerance limit of fish with the results of temperature 26,6 – 27,4°C, pH 7,4 – 7,9, and DO 6,2 – 7,7 mg/L.
Screening of Amylolytic Bacteria from Mina Padi Aquaculture in Panembangan Village, Cilongok District, Banyumas, Central Java: Screening of Amylolytic Bacteria from Mina Padi Aquaculture Ayal, Esther Lourence Brendha; Kasprijo, Kasprijo; Fitriadi, Ren; Ryandini, Dini; Nurhafid, Mohammad; Riady, Reza Muhammad; Anandasari, Mira Adyla
Journal of Aquaculture and Fish Health Vol. 13 No. 1 (2024): JAFH Vol. 13 No. 1 February 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i1.39210

Abstract

Amylolytic bacteria play an important role in the ecosystem, especially as probiotic and bioremediation agents in cultivation, as examples can be found in Mina Padi culture. The purpose of this study was to determine the amylolytic bacteria in the waters of the Mina Padi pond. Bacterial isolation began with bacterial sampling, inoculation and isolation of bacteria, calculation of the total abundance of bacteria, observation of bacterial morphology and bacterial purification, and isolation of amylolytic bacteria. The results of the isolation of amylolytic bacteria obtained 3 bacterial isolates capable of producing amylolytic enzymes, namely BA5, BA6, and BA7. The highest index of amylolytic activity was obtained by isolates of BA6 with a medium category of 2.3 cm, and the lowest index was obtained by BA5 with a weak category of 0.3 cm. The average bacterial abundance from each dilution was 2.5 x 103 CFU/mL.