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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biotropika Transformasi: Jurnal Pengabdian Masyarakat Jurnal Farmasi Sains dan Komunitas Jurnal Sains Teknologi dan Lingkungan (JSTL) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Jurnal Ilmu Kefarmasian Indonesia Jurnal Abdi Insani Bioscientist : Jurnal Ilmiah Biologi Jurnal Ilmiah Sangkareang Mataram Journal of Science and Science Education (JoSSEd) Jurnal Warta Desa (JWD) Jurnal Pendidikan dan Pengabdian Masyarakat Samota Journal of Biological Sciences (SJBIOS)
Suryadi, Bambang Fajar
PS BIOLOGI, FAKULTAS MIPA UNIVERSITAS MATARAM
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Other

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Fishmeal-Based Media Supports Growth and Endospore Production of Locally-Isolated Lysinnibacillus sphaericus and Induces its Toxicity to 3rd Instar Aedes aegypti Larvae in Laboratory Conditions Suryadi, Bambang Fajar; Mustikasari, Ika; Annisa, Zuriatun; Sarkono; Tresnani, Galuh
Jurnal Penelitian Pendidikan IPA Vol 10 No 7 (2024): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i7.7361

Abstract

The aim of this study was to determine whether fishmeal-based media could be used to grow L. sphaericus and induce its toxicity against Aedes aegypti larvae. Three concentrations (10, 20 and 30%) of fishmeal-based media were used to grow L. sphaericus isolate Bs2-1-2. Cell growth and endospore production were observed every 12 hours for 72 hours. The lethal concentration was measured every 24 hours for 72 hours of fermentation. The highest cell concentration was found in L. sphaericus grown on the media with 30% fishmeal concentration (3.03x1008 cells/mL), followed by 20% concentration (2.43x1008 cells/mL) and the lowest at 10% concentration (2.20x1008 cells/mL). At the end of fermentation, the highest concentration was found in L. sphaericus grown on 30% fishmeal-based media (1.51x1008 cells/mL), followed by 20% media (6.95x1007 cells/mL) and 10% media (3.21x1007 cells/mL). After 72-hour incubation, the highest endospore concentration was achieved by L. sphaericus grown on 20% (2.51x1008 cells/mL) and 10% (2.19x1008 cells/mL) fishmeal-based media. Initial larval toxicity of L. sphaericus showed the highest mortality on 20 and 30% fishmeal-based media (both reaching 53.33%), while 10% fishmeal-based media gave only 26.67% larval mortality. The LC50 value at 72 hours was achieved by L. sphaericus cultured on 30% fishmeal-based media (2.47 x 1008 cells/mL), followed by 20% concentration (4.82 x 1008 cells/mL) and 10% concentration (9.01 x 1009 cells/mL). The conclusion of this study was all concentrations of fishmeal-based media could support cell growth, endospore production and larval toxicity induction of L. sphaericus.
Isolation of Entomopathogenic Lysinibacillus sphaericus from Sewage at Some Housing Complex in Mataram City and Evaluation of Its Toxicity Against Aedes aegypti Larvae in Laboratory Dewi, Novia Devi Kusuma; Hidayati, Ernin; Sarkono, Sarkono; Prasedya, Eka Sunarwidhi; Suryadi, Bambang Fajar
Biotropika: Journal of Tropical Biology Vol. 10 No. 1 (2022)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2022.010.01.07

Abstract

The aims of this study were to isolate Lysinibacillus sphaericus from sewage at some housing complex in Mataram city and evaluate its toxicity against third Aedes aegypti larvae. The bacteria collected from mud taken from diluted sewage and objected to heat shock procedure at 80ºC for 30 minutes. The microbiological culture was done using a Nutrient Agar solid medium and incubated at 30ºC for 72 hours. Bacterial characterization was done based on bacterial colony morphology, cell morphology, cell physiology, and cell biochemistry characteristics. Toxicity test on 3rd Aedes aegypti larvae was done for 24, 48, and 72 hours applying Nutrient Broth medium with various dilution. From this study total of 11 isolates of Lysinibacillus sphaericus were isolated, only three isolates showed a high killing rate against Ae. aegypti in 24-hour observation. They were isolate Bs9-2-3 (LC50 24-hour value was 1.75 x 104 cell/mL), isolate Bs9-1-5 (LC50 24-hour value was 6.23 x 104 cell/mL) and isolate Bs2-1-2 (LC50 24-hour value was 7.17 x 106 cell/mL). These local isolates of L. sphaericus had good potential to be developed for bacterial-based biopesticide/biolarvicide for battling Aedes mosquito larvae in the near future.
Co-Authors Alfany, Chalida Annisa, Zuriatun Atwazzah Taisir Dewi, Novia Devi Kusuma Dining Aidil Candri, Dining Aidil Ernin Hidayati Ernin Hidayati Ernin Hidayati Ernin Hidayati ERNIN HIDAYATI Faturrahman Faturrahman Faturrahman Febrian, Novi Fitria Rizka Hidayat Galuh Tresnani Galuh Tresnani Hidayat, Hilal Aisyah I Gusti Agung Ayu Hari Triandini I WAYAN SUANA Ika Mustikasari, Ika Iman Surya Pratama Iman Surya Pratama Iman Surya Pratama ISLAMUL HADI Kurniasih Sukenti Kusuma, A. A. Ngurah Nara Kusuma, AA Ngurah Nara Kusuma, Anak Agung Nara Made Raningsih Novi Febrian Parisya Velayati Furqon Pita, Baiq Nuraini Ayu Prasedya, Eka Sunarwidhi Putri, Vita Septiana Sulaiman Putu Monik Ananta Puspitarini Putu Monik Ananta Puspitarini Ria Hapsari Sadewo, Imran Sarkono Sarkono Sidhiawan, Anak Agung Putu Siti Rahmatul Aini Sukiman Sukiman Sukiman Sukiman Sukiman Sukiman Sukiman Sukiman Taqwim, Ahsani Yuliadi Zamroni