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Junaiddin, Junaiddin
Program Studi Teknologi Laboratorium Medis; Sekolah Tinggi Ilmu Kesehatan Papua
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Dentistry Education Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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The Comparison of the Microbiological Quality of RO and Mineral Refill Drinking Water Based on Presumptive Detection of Escherichia coli Andirwana; Junaiddin; Fenti A Tupanwael; Evi Hudriyah Hukom; Bevi M Workala
JURNAL BIOEDUKASI Vol 9 No 2 (2026): Jurnal Bioedukasi Edisi Oktober 2026
Publisher : UNIVERSITAS KHAIRUN

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Abstract

The microbiological quality of refill drinking water is an important aspect in ensuring the safety of water consumed by the community. The presence of Escherichia coli can be used as an indicator of fecal contamination and is one of the important parameters in assessing the microbiological quality of drinking water. This study aims to compare the microbiological quality of reverse osmosis (RO) and mineral refill drinking water based on the presumptive detection of Escherichia coli. This study uses a descriptive observational design with a comparative approach based on laboratory examination. A total of 40 refillable drinking water samples, consisting of 20 RO samples and 20 mineral samples, were examined at the Microbiology Laboratory of STIKES Papua from May to July 2026. The examination was conducted through presumptive testing using Lactose Broth (LB), confirmatory testing using Brilliant Green Bile Broth (BGLB), and observation of colony characteristics on Eosin Methylene Blue (EMB) agar. The research results show that 8 out of 40 samples (20%) indicated presumptive positive results for Escherichia coli. In the RO group, 1 positive sample (5%) was found, while in the mineral group, 7 positive samples (35%) were found. The Fisher’s exact test results showed a p-value of 0.044 (p<0.05), indicating a difference in the proportion of presumptive Escherichia coli results between RO and mineral refill water. Descriptively, the proportion of positive results was higher in the mineral water group compared to the RO group. These findings highlight the importance of microbiological quality control and the implementation of good hygiene and sanitation practices at all stages of management and refilling of drinking water. Because the examination stopped at the EMB medium, the positive results in this study are presumptive and not yet a definitive confirmation of Escherichia coli. Further examination using biochemical tests is necessary to confirm the identity of the bacteria
Co-Authors Agung, Angelich Windy R AMIR, NURHIDAYAH Andi Sulfikar Andirwana Andirwana Astuti R Astuti R Astuti R Bevi M Workala Diva Dallas Soulisa Etnis, Baktianita Ratna Etnis, Baktianti Ratna Evi Hudriyah Hukom Evi Hudriyah Hukom Exaudian F. Lerebulan Febrina Mahmudah Fenti A Tupanwael Fenti A Tupanwael Fitriani Gunawan, Sahrul Haeril Amir Hamsinah , St Hansen Maikel Su Hasnawati Hukom, Evi Hudriyah Khusnul Sari Wahyuni Lalu Nasrulloh Lerebulan, Exaudian Flourens Letsoin, Paulina Maloga, Abdi Marasabessy, Hadija Marthen Sagrim Merlis Simon Miranda Taborat MPH Wahyuni Muhamad Faisal Arianto Muhamad Faizal Arianto Muhammad Rivaldi Mulyati Mulyati Nurfatimah, Lia Nurul Fadhilah Gani Permata, Reni PH Livana Ridha Putri Iriani Sabaniah Indjar Gama Sabila Sahib, Rahmawansyah bin Sakina Sarnia Iriani Lihawa Sakinah Sarnia Iriani Lihawa Siagian, Jenni Lilis Sidik, Muhammad Nur Sulfikar, Andi Taborat, Miranda Tupanwael, Fenti A Untari Untari Yulinda M Bambungan Yulinda Margaret Bambungan Zulfadli Zulfadli Zulfadli Zulfadli Zulihi Zulihi, Zulihi