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All Journal HAYATI Journal of Biosciences VALENSI Lantanida Journal Jurnal SOLMA JSMARTech : Journal of Smart Bioprospecting and Technology International Journal of Engineering, Science and Information Technology Makara Journal of Science Journal of Halal Science and Research Jurnal Kimia Unand Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Kartika, Irma Ratna
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Prediction of biomarker peak of infrared spectra between pork fat and chicken meat fat using fourier transform infrared spectroscopy technique and clotter plot method Saputra, Irwan; Kartika, Irma Ratna
Journal of Halal Science and Research Vol. 5 No. 2 (2024): September
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jhsr.v5i2.10820

Abstract

The increasingly widespread contamination of chicken meat by pork requires a method to identify the presence of these contaminants quickly and cheaply. Apart from analyzing the spectrum of various pure animal fats, this research also aims to vary the concentration of pork fat and chicken fat to know the FTIR peak spectrum, which will change with changes in concentration. The presence of spectral peaks that change can be identified due to changes in pork fat concentration, which also shows a typical peak spectrum position in pork fat. Variations in the concentration of pork fat contaminants in chicken fat consist of the ratio of the mixture of pork fat (PF) and chicken fat (CF), namely 90:10 (PC1), 80:20 (PC2), 70:30 (PC3), 60:40 (PC4), 50:50 (PC5), 40:60 (PC6), 30:70 (PC7), 20:80 (PC8), and 10:90 (PC9). A scatter plot, a graph usually used to see the relationship pattern between 2 variables, is employed in this research to visualize the changes in spectral peaks with varying concentrations of pork fat in chicken fat. The data scale must be an interval and ratio scale to use a scatter plot. Biomarker wavelengths were identified from the spectra of four animal fats and palm oil at positions 2948.9 and 3007 cm−1, separated by four animal fats and palm oil at a certain distance, thus indicating that these wavelengths could be used to identify non-halal samples.   Keywords: Chicken, Clotter plot, Fat, Infrared, Meat, Pork, Spectra
DETERMINATION OF OPTIMAL ANNEALING TEMPERATURE Vibrio alginolyticus PRIMERS USING POLYMERASE CHAIN REACTION METHOD Putri, Gladys Indira; Nurjayadi, Muktiningsih; Declan, Jefferson Lynford; Krisdawati, Ismaya; Juliansyah, Dandy Akbar; Fahriza, Tiara; Azzahra, Maharanianska; Maulana, Irvan; Kartika, Irma Ratna; Kurniadewi, Fera; Sukmawati, Dalia; Saamia4, Vira; Saputro, Dwi Anna Oktaviani; Wiranatha, I Made; Abomoelak, Bassam; El-Enshasy, Hesham Ali
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2976

Abstract

Food poisoning is a global issue of grave concern. If food is not properly cooked, it can be a medium for the spread of pathogenic bacteria. Vibrio alginolyticus is one of the pathogenic bacteria that can cause food poisoning. real-time Polymerase Chain Reaction (rt-PCR) can detect pathogenic bacteria in food, so it is necessary to determine the optimal annealing temperature. This research aims to obtain the optimal annealing temperature of the Va_Chr1_FR primer using Gradient PCR. The DNA concentration used was 174.5 with an A260/A280 purity of 1.94. The temperature range tested, 53°C-62°C, corresponds to the melting temperature of the Va_Chr1_FR primers. The primers designed were F5'-TTCTTCTGTTGTAGGTTCCG-F3' and R5'-CCAGCCCTCACATCTAATAC-R3'. Based on these results, a temperature of 60°C is deemed as the most optimal annealing temperature because it produces one of the brightest bands on electrophoresis with an amplicon length of 146 bp. The findings of this study will be beneficial to the development of Va_Chr1_FR Vibrio alginolyticus primers testing on food samples using the real-time PCR method. 
Pelatihan Pemanfaatan Aplikasi Gimkit untuk Guru Kimia Madrasah Aliyah (MA) di MGMP Kimia Provinsi DKI Jakarta Kartika, Irma Ratna; Kurniadewi, Fera; Widiawan, Ages Kayla Shyfa; Nugroho, Aidahaya Hanannisa; Handayani, Fitria; Dewanti, Farradilla Alenia; Irwanto, Irwanto; Susilo, Ahmad Dwi; Barkah, Eka; Saputra, Irwan; Allanas, Edith; Laurensia, Laurensia; Asworo, Yuliana Dwi; Rustini, Yeyet; Kartikawati, Nindya Galuh
Jurnal SOLMA Vol. 14 No. 3 (2025)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v14i3.20603

Abstract

Pendahuluan: Penurunan skor sains siswa Indonesia dari 403 poin (tahun 2015) menjadi 383 poin (tahun 2022) mendorong penerapan Kurikulum Merdeka berbasis digital dan inovatif. Sehingga diperlukan pelatihan pembuatan soal interaktif berbasis game online yang bertujuan membekali guru Kimia Madrasah Aliyah (MA) Provinsi DKI Jakarta yang tergabung dalam Musyawarah Guru Mata Pelajaran (MGMP) untuk menguasai pembuatan soal interaktif berbasis game online guna mempersiapkan siswa menghadapi ujian berbasis teknologi serta mengurangi kecemasan siswa. Metode: Kegiatan dilakukan secara luring dan daring pada 20 guru melalui tahap sosialisasi informasi, pelatihan, dan evaluasi. Hasil: Kegiatan pelatihan meningkatkan pemahaman dan penguasaan guru Kimia MA tentang pembuatan soal interaktif berbasis game online, yaitu Gimkit. Kesimpulan: Kegiatan ini berhasil meningkatkan kompetensi pedagogik guru Kimia MA dalam pemanfaatan teknologi, serta berkontribusi terhadap peningkatan kualitas pembelajaran di MGMP melalui penggunaan aplikasi Gimkit.
Pelatihan Pemanfaatan Limbah Kulit Buah Naga sebagai Antioksidan dalam Pembuatan Lip balm di Kabupaten Majalengka Hati, Futi Kusuma; Kartika, Irma Ratna; Saputra, Irwan; Listiara, Aulia; Aurellia, Dema Griseldis; Aisyafitri, Afifah; Azahra, Nur Fadillah Adelia; Albana, Fadhila Shofi
Jurnal SOLMA Vol. 14 No. 3 (2025)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v14i3.20994

Abstract

Background: Kulit buah naga merupakan limbah organik yang kaya akan antosianin dan berpotensi sebagai antioksidan alami untuk perawatan kulit, namun pemanfaatannya dalam produk perawatan kulit, seperti lip balm masih terbatas. Studi ini bertujuan memberikan sosialisasi dan pelatihan kepada siswa SMP di Kabupaten Majalengka mengenai inovasi pemanfaatan limbah kulit buah naga dalam pembuatan lip balm. Metode: Kegiatan mencakup sosialisasi manfaat buah naga dan lipbalm, demonstrasi, praktik, diskusi tanya jawab dan evaluasi. Sebanyak 43 siswa SMPIT Tazkia Insani Majalengka mengikuti kegiatan ini. Para peserta dilatih mengelola limbah kulit buah naga menjadi lip balm dan mempraktikannya langsung sehingga turut mengurangi sampah di lingkungan sekitar dan menumbuhkan kreativitas serta keterampilan dalam menciptakan produk ramah lingkungan. Hasil: Peserta berhasil membuat lipbalm dari limbah kulit buah naga serta memahami proses dan bahan yang digunakan. Kesimpulan: Program penguatan sistem informasi website mampu meningkatkan aksesibilitas informasi dan partisipasi warga RT 39. Keberhasilan ini menunjukkan pentingnya kolaborasi antara pengurus RT dan kelompok ibu rumah tangga dalam mewujudkan masyarakat yang adaptif terhadap teknologi.
Deteksi Dini Diabetes Melitus dan Edukasi Kesehatan bagi Guru Kimia Madrasah Aliyah, Jakarta Wulandari, Meyliana; Kartika, Irma Ratna; Ramadani, Rosi Fitri
Jurnal SOLMA Vol. 15 No. 1 (2026)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v15i1.21581

Abstract

Pendahuluan: Diabetes melitus (DM) merupakan penyakit kronis yang terus meningkat dan membutuhkan upaya deteksi dini serta edukasi yang komprehensif, terutama bagi kelompok berisiko seperti guru. Program Pengabdian kepada Masyarakat ini bertujuan untuk meningkatkan pengetahuan dan kesadaran guru Kimia MA MGMP Provinsi DKI Jakarta melalui deteksi dini DM, serta edukasi penerapan pola makan sehat. Metode: Pemeriksaan kadar glukosa darah, pretest dan postest pengetahuan DM, serta penyampaian materi interaktif oleh dosen. Hasil: Hasil menunjukkan bahwa 25–40% peserta pada tiap kelompok usia memiliki kadar glukosa darah puasa >126 mg/dL yang mengindikasikan tingginya risiko DM (ambang batas normal puasa <100 mg/dL). Perbandingan nilai pretest dan postest berdasarkan uji-t memperlihatkan peningkatan pengetahuan peserta (p<0,001), sementara survei umpan balik menunjukkan lebih dari 96% peserta merasa kegiatan ini sangat bermanfaat, relevan, dan aplikatif. Kesimpulan: Kegiatan ini terbukti efektif dalam meningkatkan literasi kesehatan guru serta mendorong praktik hidup sehat di lingkungan sekolah. Program ini juga mendukung capaian IKU-PTN dan SDG 3 terutama pada tujuan kesehatan dan kesejahteraan.
CHEMICAL PROFILING AND ANTIBACTERIAL ACTIVITY OF Nannochloropsis oculata EXTRACTS AGAINST FOODBORNE PATHOGENS Hati, Futi Kusuma; Nurjayadi, Muktiningsih; Kartika, Irma Ratna; Azahra, Nur Fadillah Adelia; Albana, Fadhila Shofi; Aisyafitri, Afifah; Aurellia, Dema Griseldis
Lantanida Journal Vol 14 No 1 (2026): January-June 2026
Publisher : UIN AR-RANIRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/f2h2ht06

Abstract

Foodborne illnesses remain a public health concern in Indonesia, prompting the search for natural antibacterial agents. This study evaluated the antibacterial activity and chemical profile of Nannochloropsis oculata extracts against Listeria monocytogenes and Shigella flexneri. Microalgae were cultivated, and cell density was monitored using an Ultraviolet–Visible (UV–Vis) spectrophotometer. Biomass was harvested and extracted using chloroform–methanol, followed by compound identification through Gas Chromatography–Mass Spectrometry (GC–MS). Antibacterial activity was assessed using the disc diffusion method. A total of 1.3196 g of dry biomass was obtained. At 200 mg/mL, the extract produced inhibition zones of 8.40 ± 0.17 mm against L. monocytogenes and 8.83 ± 0.29 mm against S. flexneri. GC–MS analysis revealed fatty acid derivatives, predominantly methyl palmitoleate (26.47%). These findings provide preliminary insight into the antibacterial potential of N. oculata extracts; however, further quantitative and application-based studies are required to determine their practical relevance.
Co-Authors AA Sudharmawan, AA Aboemolak, Bassam Abomoelak, Bassam Abumoelak, Bassam Ade Danova Agus Setiawan Aisyafitri, Afifah Akbar, Dandy Albana, Fadhila Shofi Allanas, Edith Angelina, Helzi Anggraeni, Rosita Gio Anis, Mhd. Aqilah, Amadita Shafa Asworo, Yuliana Dwi Aurellia, Dema Griseldis Azahra, Nur Fadillah Adelia Azizah, Puan Aqila Azzahra, Maharanianska Barkah, Eka Baso Maruddani Berkahingrum, Ayu Chen, Shyi-Tien Dalia Sukmawati Danova, Ade Dewanti, Farradilla Alenia El Enshasy, Hesham El-Enshasy, Hesham Ali Enshasy, Hesham Ali El Fahriza, Tiara Farida, Tania Fitria Handayani Fitriyanti, Anisa Grace, Grace Hati, Futi Kusuma Hermawati, Elvira Irsyad, Daffa Ihsanullah Irwanto Irwanto Jefferson Lynford Declan Juliansyah, Dandy Akbar Kartikawati, Nindya Galuh Krisdawati, Ismaya Kurniadewi, Fera Laurensia, Laurensia Listiara, Aulia Maulana, Irvan Meyliana Wulandari Muktiningsih Nurjayadi Musie, Royna Rahma Nugroho, Aidahaya Hanannisa Pramono, Eko Kuncoro Putri, Adinda Myra Amalia Putri, Gladys Indira Putri, Gusti Angieta Rafiuddin Syam Rahmawati, Atikah Nur Rosi Fitri Ramadani Rustini, Yeyet Saamia, Vira Saamia4, Vira Saputra, Irwan Saputro, Dwi Anna Oktaviani Shangkara, Muhammad Arkent Sihombing, Ananda Indah Putri Siti Fatimah SRI RAHAYU Susilo, Ahmad Dwi Syafriani, Yolanda Febrica Widiawan, Ages Kayla Shyfa Wiranatha, I Made