Garuda - Garba Rujukan Digital

p-Index From 2020 - 2025

1.152

P-Index

This Author published in this journals

All Journal Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Partner Jurnal Riset Akuakultur Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Pengembangan Penyuluhan Pertanian Jurnal Ilmu Peternakan Terapan Jurnal Kedokteran Hewan Jurnal Medika Veterinaria

Joko Prastowo

Departemen Parasitologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta

Author-ID : 1470402

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences Veterinary Other

Published : 46 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3 4 5

Daya Ovisidal dan Larvisidal Infusa Daun Bambu Apus (Gigantochloa apus)Terhadap Haemonchus Contortus Secara in Vitro Budi Purwo Widiarso; Wisnu Nurcahyo; Kurniasih Kurniasih; Joko Prastowo
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Gastrointestinal Nematodes are one among cause decrease production efificiency in goat and sheep in Indonesia, especially parasite of abomasum namely Haemonchus contortus. This parasite causes disease of Haemonchosis causing anemia, and causes mortality to young goat or sheep. Loss of economics caused by high morbidity and mortality this parasite so that can improve the expense of prevention and medication of this disease. This research aim was to evaluate ovicidal and larvicidal apus bamboo leaf infusion (Gigantochloa apus) against Haemonchus contortus by in vitro. Examination of apus bamboo leaves infusion to seven concentrations namely : 1.2; 1.0; 0.8,; 0.6; 0.4; 0.2;0.1; 0.05 and 0 mg / ml as negative control. Positive control uses Albendazole with concentration 0,025 mg / ml. The highest concentration 1.2 mg / ml apus bamboo leaves infusion inhibited 92.35% egg hatching ( LC 0.49 mg / ml ) and have larvicidal activity equal to 90% ( LC50 0,47mg / ml). The concentration 1.2 mg / ml also have similar effect with positive control which use Albendazole. Phytochemical apus bamboo leaf analysis of the highest concentration revealed the presence tanin condensed, saponins, and alkaloids.

Pengaruh Serbuk Jambe (Areca catechu) Dibandingkan Serbuk Gabungan Jambe (Areca catechu) dan Binahong (Anredera cordifolia) Terhadap Ascariasis pada Ayam Buras Okti Herawati; Kurniasih Kurniasih; Joko Prastowo
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Infestasi Ascaridia galli mengakibatkan penurunan produktivitas dari ayam buras baik daging maupun telur. Pengendalian infestasi A. galli dilakukan menggunakan tanaman yang mengandung antiparasit sebagai anthelmintik. Penelitian ini bertujuan untuk mengetahui efek pemberian serbuk Jambe dan serbuk gabungan Jambe dengan Binahong terhadap infestasi Ascaridia galli pada ayam buras berdasarkan jumlah egg per gram (epg) feses, berat badan, jumlah cacing A.galli, dan perubahan histopatologi usus. Hewan yang digunakan dalam penelitian ini ialah ayam buras yang berumur 2 bulan dan positif terinfestasi cacing Ascaridia galli. Dilakukan penghitungan jumlah egg per gram (epg) dan berat badan sebelum perlakuan. Sebanyak 10 ekor ayam buras yang terbagi ke dalam 2 kelompok. Kelompok 1 diberi serbuk Jambe dengan dosis 0,1mg/kg BB sedangkan kelompok 2 diberi serbuk gabungan Jambe 0,05mg/kg BB dan Binahong 0,0125mg/kg BB. Pengobatan dilakukan secara per oral selama 7 hari, pada hari ke 10 dilakukan eutanasi. Data hasil perhitungan jumlah egg per gram (epg), jumlah cacing, berat badan dan perubahan histopatologi dianalisa secara diskriptif. Pemberian serbuk Jambe maupun serbuk gabungan Jambe dan Binahong dapat menurunkan jumlah epg, meningkatkan berat badan ayam dan membunuh cacing A.galli sebesar 80% untuk kelompok 1 dan 40% untuk kelompok 2. Perubahan histopatologis usus halus terlihat adanya infiltrasi sel radang berupa eosinofil dan limfosit di duodenum, jejunum dan ileum serta terdapat potongan cacing cestoda pada kedua kelompok. Serbuk Jambe lebih berpotensi sebagai anthelmentik dibandingkan serbuk gabungan Jambe dan Binahong dilihat dari penurunan jumlah epg, peningkatan berat badan dan jumlah cacing pada usus. Pemberian serbuk Jambe maupun serbuk gabungan Jambe dan Binahong dapat membunuh nematoda tetapi tidak untuk cestoda.

Identification of Clinostomum complanatum (DIGENEA: CLINOSTOMIDAE) Infecting Anabas tes Morina Riauwaty; Kurniasih Kurniasih; Joko Prastowo
Jurnal Sain Veteriner Vol 29, No 2 (2011): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The aim of study was to molecularly determine Clinostomum complanatum (Digenea: Clinostomidae) from freshwater fish in Yogyakarta and Riau, Indonesia.

Pengaruh Infusa Biji Buah Pinang (Areca catechu) Terhadap Tingkat Kematian dan Morfometri Cacing Dewasa Ascaridia galli Secara In Vitro Wida Wahidah Mubarokah; Kurniasih Kurniasih; Wisnu Nurcahyo; Joko Prastowo
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The study evaluates in vitro effects of Areca catechu crude aqueous extract as anthelmintics on Ascaridia galli adult mortality rate and morphometry. Naturally infected chickens were collected from slaughtered chickens at local slaughterhouses in Yogyakarta. Chicken’s intestines were carefully examined and transported to the Parasitological Laboratory of Gadjah Mada University, Yogyakarta. Ascaridia galli were collected from intestines (including duodenum, jejunum, and ileum) and put into a petri dish containing 0.62% saline water. There were 60 A. galli in each concentration of Areca catechu crude aqueous extract. Investigation on mortality rate of adult worms at various concentration and observation as well as differences in A. galli morphometry and mortality rate were analyzed using analysis Anova. The figures of the parasites or the parts of the parasites were captured using camera Lucida and then they were measured using both micrometer and curvimeter. The morphology of the parasites was identified to find out their morphometric characteristics.The results of morphometric observation of the A. galli showed that there was a significant difference in the body width and the vulva length of the females and in the body width, the esophageal length and the esophageal width of the males.This research disclosed that Areca catechu crude aqueous extract significantly affected the mortality rate of adult A.galli at various concentration. This research observed that the best concentration to kill the adult worms was 25% Areca catechu crude aqueous extract.

Tingkat Kejadian Infestasi Parasit Saluran Pencernaan pada Ayam Buras di Rumah Pemotongan Mbok Sabar dan Pasar Terban Yogyakarta Dyah Nur Hidayati; Joko Prastowo
Buletin Peternakan Vol 28, No 1 (2004): Buletin Peternakan Vol. 28 (1) Februari 2004
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v28i1.1489

Artikel dalam bentuk PDF

Molecular Detection of Toxoplasmosis Using Specific Primers P30, B1, and rDNA Wisnu Nurcahyo; Joko Prastowo; Ana Sahara
Jurnal Veteriner Vol 13 No 1 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (137.706 KB)

Study in order to develop molecular techniques using specific primers for the early diagnosis oftoxoplasmosis have been conducted. Detection of Toxoplasma gondii genome was performed usingpolymerase chain reaction (PCR) technique. The primers used in this study were rDNA, P30, and B1. ThePCR products were further run using gel electrophoresis (gel 1.5% – 2.0%) and the band was documented.Toxoplasma was detected at 500 bp and 600 bp using primer P30 and B1, respectively. Whereas usingprimer rDNA no band was observed. It was assumed that primer rDNA was not sensitive since the targetamplification was 88 bp.

Strongyloides spp Distribution on Orangutans in Tanjung Putting National Park, Care Center in Pangkalanbun, and Sebangau National Park Wisnu Nurcahyo; Joko Prastowo
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (161.949 KB)

Strongyloides spp is a parasitic nematode in livestock, primate and human which is considered asa danger zoonotic disease. Therefore, study about parasite distribution is very important in order to find outgenetic diversity among orangutan in quarantine, zoo and nature, as an effort to explore infection patternand life cycle of Strongyloides spp on orangutan. Amount of 326 orangutan feces were taken from threedifferent habitat of orangutan in Central Borneo, Tanjung Puting National Park, Orangutan Care Centerand Sebangau National Park. Samples which were collected from Tanjung Puting, Care Center and Sebangauwere 75, 80 and 171 respectively. Those samples were transported to the Parasitology laboratory in Facultyof Veterinary Medicine, Gadjah Mada University, Yogyakarta for examination and detection. Prevalence ofstrongyloides in Tanjung Putting, Sebangau and Orangutan Care Center were 24%, 14,6% and 13,3%respectively. Among positive samples of Strongyloides, 62,5% were from male orangutans, while 37,5% werefrom female orangutans. Strongyloides in pre adult and baby orangutan were 91,6% and 4,2% respectively.Meanwhile, Strongyloides in adult orangutan were very rare. Orangutan habitat in Sebangau National Parkis an ideal habitat for orangutan, supported by the watery condition of peat land, so that Strongyloides re-infection become difficult. Some factors may have important role in Strongyloidoses, such as behavior,physical condition, nutrition, age, body weight, sex, immunity and social status of orangutan.

Daya Larvasida Ekstrak Daun Muda Kedondong Hutan Terhadap Haemonchus contortus Secara In-vitro (LARVICIDAL EFFECT OF SPONDIAS PINNATA LEAF EXTRACT AGAINST HAEMONCHUS CONTORTUS IN VITRO) I Gusti Komang Oka Wirawan; Wisnu Nurcahyo; Joko Prastowo; Kurniasih Kurniasih
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study aimed to asses effective concentration of condensed tannins in the extract of Lannea grandis leaves (EDMKH) as larvacide anti-helminths on Haemonchus contortus. Experimental design used in this study was completely randomised design. The treatments group were administrated with 2.5%, 3.5% , and 4.5% EDMKH in the 0.2 g/mL solution, respectively. Moreover aqueduct was used for the negative control group, while 0.055% albendazole was applied to the positive control group. Variables measured were percentage of developed and undeveloped L1 in the incubation media. The data were the statistically analysed following General Linear Model format for completely randomised design. The software used in the analysis was SPSS v. 23.0 (IBM-SPSS 2015). Results showed that among the EDMKH treatments, the lowest larvacidal effect was recorded for 2.5% EDMKH treatment compared with 3.5% and 4.5% EDMKH. However, the value achieved by 2.5% EDMKH treatment was significantly higher (P<0.05) than that of negative control treatment. The highest larvacidal effect (100%) was shown by 4.5% EDMKH treatment which was not significantly different (P>0.05) from the positive control treatment. ABSTRAK Penelitian ini bertujuan untuk mengetahui konsentrasi efektif tanin kondensasi di dalam Ekstrak Daun Muda Kedondong Hutan (EDMKH) sebagai antelmintik yang bersifat larvasida terhadap Haemonchus contortus (H. contortus) secara in-vitro. Rancangan penelitian yang digunakan dalam penelitiam ini adalah Rancangan Acak Lengkap (RAL), dengan lima perlakuan yaitu tiga perlakuan EDMKH konsentrasi: 2,5%, 3,5%, 4,5% dari 0,2 g/mL larutan tersedia, satu kelompok kontrol (-) menggunakan air suling, satu kelompok kontrol (+) menggunakan albendazole konsentrasi 0,055%. Variabel yang diukur dan dianalisis: jumlah persentase L1 yang berkembang dan tidak berkembang dalam kelompok rendaman. Data persentase daya larvasida H. contortus dari konsentrasi tanin kondensasi EDMKH secara in-vitro dianalisis menggunakan analisis varian mengikuti prosedur Rancangan Acak Lengkap (RAL) menggunakan SPSS Versi 23(IBM-SPSS 2015). Hasil analisis statistika menunjukkan bahwa persentase larvasida EDMKH 2,5% nyata lebih rendah dibandingkan 3,5%, 4,5% dan albendazole 0,055% (P<0,05) tetapi lebih tinggi dari kontrol negatif. Konsentrasi efektif EDMKH terhadap larva H. contortus adalah konsentrasi 4,5%, memberikan efektivitas larvasida 100%, dan tidak berbeda nyata dengan kontrol positif (P>0,05).

Pengembangan Antibodi Poliklonal dari Stadium Oosista, Sporosista, dan Sporozoit Eimeria tenella (THE DEVELOPMENT OF POLYCLONAL ANTOBODY FROM EIMERIA TENELLA OOCYST, SPOROCYST, AND SPOROZOITE STADIUM) Galuh Tresnani; Joko Prastowo; Wisnu Nurcahyo; Budi Setiadi Daryono
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.074 KB)

The research on developing diagnostic method, vaccine, and drugs for coccidiosis has been focused onthe finding of the immunogenic molecule in Eimeria. The identification of this agent will need the antibodywhich can recognize the biomolecule in the antigen. Antibody that has been developed for this purposeshould be analyzed first, and one of the simple methods for analyzing this antibody is through dot blotanalysis. The objective of this research was to analyze the polyclonal antibody which developed from theoocyst, sporocyst, and sporozoite of E. tenella using dot blot analysis. The antigen for this polyclonalantibody was made from each of the E. tenella stadium by sonication. Fifteen mice, divided into 3 groups,were then injected subcutaneously with each antigen. The sera from these mice were then collected, analyzedby using ELISA and then it will be used for the dot blot analysis. The research result showed that thepolyclonal antibody which has been developed in mice from each antigen can react with the antigen itself.From this result it can be concluded that the developing of this antibody is successful and it can be used forfurther research in immunoproteomic.

Diagnosis Toksoplasmosis Kongenital Berdasarkan Gen Surface Antigen-1 Toxoplama gondii Isolat Lokal Menggunakan Polymerase Chain Reaction (DIAGNOSIS OF CONGENITAL TOXOPLASMOSIS BASED ON SURFACE ANTIGEN -1 GENE OF LOCAL ISOLATE TOXOPLASMA GONDII USING POLY Dwi Priyowidodo; Sri Hartati; Asmarani Kusumawati; Joko Prastowo
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.315 KB)

Congenital toxoplasmosis has an important role in the transmission of toxoplasmosis in animals andhumans. Thus, a rapid and an accurate diagnostic method is needed. The aim of this study was to conductthe diagnosis technique of congenital toxoplasmosis in mice based on surface antigen-1 (SAG-1) gene oflocal isolates (IS-1) T. gondii using Polymerase Chain Reaction (PCR). A total of 15 pregnant mice Balb/C strain with the aged of eight weeks were used as experimental animal. Mice were intraperitoneallyinfected with 103tachizoit of T. gondii RH strain at day 9th of gestation. Amniotic fluids, blood, fetus, andplacenta then were collected at day 1, 2 , 3, 4 and 5 post infection. DNA was extracted from the abovesamples using PureLinkTM Genomic DNA Kit (Invitrogen, Life Technologies, US), and then amplified byusing specific primer based on SAG-1 gene of the local isolate T. gondii. This study shows that positivePCR result were seen in all samples of amniotic fluids at day 2 up to day 5 post infection. Fetus andplacenta samples also show positive PCR result at 3 up to day 5 post infection. Negative PCR result showsin blood samples, however. To conclude, PCR technique using SAG-1 gene of local isolates T. gondii as atarget gene, could be used to detect congenital toxoplasmosis from infected mouse samples such as, amnionfluids, fetus, and placenta. Further research was needed to apply the PCR method with SAG-1 gene of localisolate T. gondiion the human samples of congenital toxoplasmosis.

Co-Authors . Dwilantiani . Rianawati Alek Arisona Alfarisa Nururrozi Ambar Pertiwiningrum Ana Sahara Ana Sahara April Hari Wardhana Argamjav, Bayanzul Asmarani Kusumawati Awaludin, Aan Bambang Ariyadi Bambang Ariyadi, Bambang Bambang Sutrisno Budi Purwo Widiarso Budi Purwo Widiarso BUDI SETIADI DARYONO Catur Sugiyanto Dwi Priyowidodo Dyah Nur Hidayati Eryl Sri Rohayati Galuh Tresnani Herawati, Okti I Gusti Komang Oka Wirawan I Gusti Komang Oka Wirawan, I Gusti Komang Imron Rosyadi Kurniasih . Kurniasih . Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Lily Gunawan Margaretha Arnita Wuri Mufasirin MUHAMAD, NUR Muhammad Hanafiah Nor Wijaya, Rendra Nugraheni, Yudhi Ratna Nugraheni, Yudhi Ratna Priyo Sambodo Priyowidodo P Putra, Zidhandika Nagita R Wisnu Nurcahyo R. Wasito . Raden Wisnu Nurcahyo Ratna Nugraheni, Yudhi Rini Widayanti Sahara, Ana Sitarina Widyarini Soedarmanto Indarjulianto Soedarmanto Indarjulianto Sri Hartati Sudarmanto Indarjulianto Sumartono Sumartono Sumartono Sumartono Sunandjak . Surya Widyarsi Teguh Ari Prabowo Wida Wahidah Mubarokah Wida Wahidah Mubarokah Widiarso, Widiarso, B. P. Windarti . Windarti Windarti Wisnu Nurcahyo Wisnu Nurcahyo Wisnu Nurcahyo Wisnu Nurcahyo Yanuartono Yanuartono . Yanuartono Yanuartono Yosua Lede Pono

Title

Found 46 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 46 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 46 Documents
Search