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Joko Prastowo
Departemen Parasitologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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IDENTIFIKASI TOKSOPLASMOSIS PADA FESES KUCING SECARA MIKROSKOPIS DAN SEROLOGIS Wisnu Nurcahyo; Joko Prastowo; Priyowidodo P
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.657 KB) | DOI: 10.21157/j.ked.hewan.v8i2.2639

Abstract

Penelitian ini bertujuan mengetahui tingkat prevalensi Toxoplasma pada kucing dan menyusun basis epidemiologis pada kucing yang sangat penting dalam peranannya sebagai hospes definitif toksoplasmosis. Sampel serum darah dan feses kucing digunakan dalam penelitian ini. Metode pemeriksaan menggunakan card agglutination Toxoplasma test (CATT) Pastorex™ Toxo kit dan metode sentrifus. Data yang dip eroleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa tingkat prevalensi Toxoplasma dengan pemeriksaan serum darah kucing sebesar 6,8% dan pemeriksaan feses sebesar 9,4%. Infeksi toksoplasmosis yang terjadi pada kucing secara umum dari pemeriksaan klinis tidak mempunyai gejala yang spesifik.
THE EFFECT OF APUS BAMBOO (Gigantochloa apus) LEAVES INFUSION TO MORTALITY RATE AND MORPHOMETRY OF Haemonchus contortus ADULT WORM IN VITRO Budi Purwo Widiarso; Wisnu Nurcahyo; Kurniasih Kurniasih; Joko Prastowo
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.488 KB) | DOI: 10.21157/j.ked.hewan.v11i4.8167

Abstract

This present project was carried out to determine the effect of apus bamboo (Gigantochloaapus )leaves infusion to mortality rate and morphometry of Haemonchus contortus adult worms in vitro. The formulated tools were collected through camera, 3 ml syringe, object glass, deck glass, mortar, drip dropper, waterbath, microscope, stopwatch, counter check, oven, erlenmeyer flask, electric scale, surgical scissor, and pipette. The allocated materials used in this studywere bamboo leaves infusion, Haemonchus contortus adult worms, aquadestilata, ethanol and NaCl 0.62%. The research was firstly concernedwith making 0.1% and 1% apus bamboo leaves infusion. The samples were obtained from Haemonchus contortus collection. Adult worms were obtained directly from abomasum of naturally infected goats that were cut at Animal Slaughter House (RPH). To investigate the matter, mortality rates of adult worms at various doses and times of observation as well as differences in Haemonchus contortus morphometry were analyzed using ANOVA. The findings disclosed that apus bamboo leaves infusion demonstrated a significant difference in the mortality rate of adult worms Haemonchus contortusat various doses and times of observation. This report presented the findings of research that the best dose to increase the mortality of worms was 1% bamboo leaves infusion. Moreover, apus bamboo leaves infusion affected the morphometry of Haemonchus contortus adult worms, especially in body length, cervical papilla width, and spicules length in males, and body length, cervical papilla width, and vulvar length in females.   
MORPHOLOGY AND MORPHOMETRY OF Haemonchus contortus IN GOATS IN YOGYAKARTA, INDONESIA Priyo Sambodo; Joko Prastowo; Sudarmanto Indarjulianto; Kurniasih Kurniasih
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (282.451 KB) | DOI: 10.21157/j.ked.hewan.v12i3.11026

Abstract

This research was carried out to determine the morphology and morphometry of Haemonchus contortus in goats. Adult female parasites were collected from abomasum of naturally infected goats and stored in lactophenol. Images of the parasite were acquired using lucida camera and measurement was performed using Axiovision LE software. Basic characters of the morphology and morphometry of Haemonchus spp. were identified by determining the value of the discriminant function of three parameters on spicule. Vulvar morphology was identified by vulvar flap shape. Data were presented in terms of mean, standard deviation, and percentage. A hundred percent of the parasites were H. contortus with discriminant function value 0.63. The size of female parasites was longer and wider (25.5-32.6 mm; 0.38-0.63 mm) than that of male ones (17.3-20.0 mm; 0.24-0.33 mm). There were only 2 varieties of vulvar flap, linguiform (81%), and knobbed (19%). There were 5 subclasses identified in the linguiform type, including linguiform A (39%), linguiform B (22%), linguiform C (17%), linguiform I (1%), and a new subclass of linguiform D (2%). Based on the the type of vulvar flap, H. contortus found in goats in Yogyakarta were different to those found in previous researches. The findings could be used in identifying the parasite species in small ruminants.
IN VITRO DEVELOPMENT OF Ascaridia galli EGGS INTO INFECTIVE EGGS AND LARVAE OF STADIUM 2 (L2) Wida Wahidah Mubarokah; Kurniasih Kurniasih; Wisnu Nurcahyo; Joko Prastowo
Jurnal Kedokteran Hewan Vol 13, No 1 (2019): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (222.993 KB) | DOI: 10.21157/j.ked.hewan.v13i1.12978

Abstract

The study aimed at finding out the development of Ascaridia galli (A. gall) eggs that were given aerator treatment and those without aerator treatment into infective eggs and L2 through in vitro culture. Each treatments has 108,000 eggs assigned to 8 groups of 1,000; 2,000; 3,000; 4,000; 5,000; 6,000; 7,000; and 8,000 eggs, respectively with 3 repetitions. Female A. galli were collected from the small intestinal lumen of naturally infected domestic chickens. The eggs collected from the uterus of adult female A. galli were incubated in sterile aquadest at ambient temperature for 45 days (without aerator) and 25 days (with aerator) to obtain the infective eggs and the L2. The number of the infective eggs and hatched L2 were counted under stereo microscope. Data were analysed descriptively. There were 97.740 eggs (90.5%) in the groups without aerator developed into infective eggs and 77,040 eggs (71.3%) developed into the L2. Meanwhile, there were 101,847 eggs (94.3%) in the groups with the aerator developed into the infective eggs and88.722 eggs (82.15%) hatched L2. It is concluded that the eggs collected from worms uterus had high viability and the aerator application shortened the developing period of the A. galli worms.
Kejadian Fascioliasis pada Sapi Perah di Kecamatan Tegalombo, Kabupaten Pacitan Alek Arisona; Soedarmanto Indarjulianto; Catur Sugiyanto; Ambar Pertiwiningrum; Joko Prastowo; Yanuartono .; Alfarisa Nururrozi; Margaretha Arnita Wuri; Teguh Ari Prabowo
Jurnal Sain Veteriner Vol 41, No 2 (2023): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.70602

Abstract

Fascioliasis adalah penyakit cacing hati yang dapat menyerang sapi dan sangat merugikan, karena dapat menurunkan produktivitas. Peternakan sapi perah di Tegalombo, Pacitan merupakan peternakan rakyat yang pengelolaannya banyak melibatkan perempuan. Penelitian ini bertujuan mengidentifikasi fascioliasis pada sapi perah di kecamatan Tegalombo, kabupaten Pacitan. Sebanyak 50 ekor sapi perah digunakan sebagai sampel di dalam penelitian ini. Semua sapi diperiksa secara fisik, diambil fesesnya dan diperiksa adanya telur cacing Fasciola sp. Hasil pemeriksaan fisik sapi secara klinis menunjukkan bahwa semua sapi pada penelitian ini menunjukkan kondisi umum normal, nafsu makan baik, serta tidak ada abnormalitas yang signifikan pada semua bagian tubuh, sehingga dinyatakan sehat secara fisik. Hasil pemeriksaan sampel feses didapatkan adanya telur cacing Fasciola sp. pada 2 dari 50 ekor sapi (4%), sedangkan 48 ekor sapi lainnya (96 %) tidak ditemukan telur cacing. Kesimpulan dari peneltian ini adalah kejadian fascioliasis pada sapi perah di Tegalombo, kabupaten Pacitan rendah. Pemeriksaan secara periodik terhadap kemungkinan fascioliasis perlu dilakukan, sebagai langkah penanggulangan fascioliasis di daerah tersebut.
Detection of malaria in goats and sheep Yudhi Ratna Nugraheni; April Hari Wardhana; Aan Awaludin; Joko Prastowo; Surya Widyarsi; Yosua Lede Pono
Jurnal Ilmu Peternakan Terapan Vol 7 No 1 (2023): Jurnal Ilmu Peternakan Terapan
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/jipt.v7i1.4372

Abstract

Plasmodium species, as the causative agent of malaria, are a significant concern in livestock production. This study aims to investigate the presence of malaria parasites in small ruminants comprised of goats and sheep. Plasmodium infections in ungulates do not cause malaria in humans. However, they might have anopheline mosquitoes as the same vector of this parasite. This study uses molecular techniques, nested PCR, to detect Plasmodium infections in small ruminants, as traditional microscopic examination may lead to misdiagnosis. The results show that Plasmodium infections were found in goat samples from Sumba Barat Daya, Nusa Tenggara Timur, with a prevalence of 7.41% based on nested PCR assay. Notably, Plasmodium was not detected through microscopic examination, underscoring the sensitivity of molecular techniques. It should be noted that none of the goats tested positive for malaria based on microscopic examination, suggesting extremely low parasitemia levels. This study emphasizes the importance of understanding Plasmodium infections in small ruminants, shedding light on their impact on animal health and their potential role in local transmission patterns. The sample size in this study is limited. Further research with larger samples and expanded geographical scope is recommended to comprehensively understand Plasmodium prevalence in small ruminants. 
PENGARUH INFEKSI CACING Ascaridia galli TERHADAP GAMBARAN DARAH DAN ELEKTROLIT AYAM KAMPUNG (Gallus domesticus) Joko Prastowo; Bambang Ariyadi
Jurnal Medika Veterinaria Vol 9, No 1 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i1.2986

Abstract

Penelitian ini bertujuan mengetahui pengaruh infeksi telur cacing Ascaridia galli (A. galli) terhadap elektrolit dan gambaran darah ayam kampung (Gallus domesticus). Kelompok perlakuan dilakukan infeksi telur berembrio cacing A. galli sebanyak 500 telur cacing/ekor ayam. Sampel berupa feses ayam untuk pemeriksaan parasitologi dan darah untuk pemeriksaan elektrolit dan pemeriksaan darah rutin. Infeksi cacing A.galli menyebabkan penurunan kadar kalium serum pada hari ke-21 dan 28 setelah infeksi (P0,05), kenaikan kadar magnesium serum pada hari ke-21 dan 28 setelah infeksi (P0,05) dan tidak memberikan pengaruh terhadap kadar natrium serum setelah infeksi. Hasil penelitian ini menyebabkan penurunan terhadap jumlah eritrosit pada hari ke-7 dan 14 setelah infeksi (P0,05), penurunan terhadap nilai packed cell volume (PCV) pada hari ke-14 setelah infeksi (P0,05), kenaikan nilai total protein plasma pada hari ke-7 setelah infeksi (P0,05), kenaikan nilai absolut sel eosinofil pada hari ke-14 setelah infeksi (P0,05), tidak memberikan pengaruh terhadap kadar hemoglobin, jumlah leukosit, nilai absolut sel heterofil, limfosit, dan monosit. Pada hari ke-28 setelah infeksi, rerata cacing yang hidup yaitu 13 ekor cacing. Dari hasil penelitian disimpulkan bahwa, infeksi 500 telur cacing berembrio A. galli menyebabkan penurunan kadar kalium, kenaikan kadar magnesium, penurunan terhadap jumlah eritrosit, penurunan terhadap nilai PCV, kenaikan nilai total protein plasma, kenaikan nilai absolut sel eosinofil, dan tidak memberikan pengaruh terhadap natrium, kadar hemoglobin, jumlah leukosit, nilai absolut sel heterofil, nilai absolut limfosit, dan nilai absolut monosit.
Subclinical anaplasmosis infection on Ettawa Goats from Kaligesing, Purworejo, Central Java, Indonesia Rosyadi, Imron; Nor Wijaya, Rendra; Ratna Nugraheni, Yudhi; Prastowo, Joko; Yanuartono; Argamjav, Bayanzul; Indarjulianto, Soedarmanto
Jurnal Ilmu Peternakan Terapan Vol 8 No 1 (2024): Jurnal Ilmu Peternakan Terapan
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/jipt.v8i1.5432

Abstract

Small ruminant anaplasmosis is a blood-parasitic disease that can cause reproductive and economic problems, especially in Ettawa crossbred. In Indonesia, especially in Central Java Province, the study of this pathogen is still limited. This study examines subclinical anaplasmosis in Ettawa crossbred goats from central Java, Indonesia. Twenty adult goats underwent comprehensive physical and laboratory examinations. The physical exams assessed factors like sex, body temperature, heart rate, respiration, and rumen function. Laboratory testing involved blood smears to detect Anaplasmaspecies and complete blood counts using a hematology analyzer. The results showed that 8 goats were positive for Anaplasma, with 2 samples resembling A. phagocytophilum and 6 resembling A. marginale. Females were more likely to be infected. Infected goats did not exhibit significant changes in vital signs compared to healthy goats. However, infected goats had lower red blood cell counts, hemoglobin, and hematocrit, as well as higher white blood cell counts, indicating subclinical anemia. This is the first study to demonstrate the impact of subclinical Anaplasma infection on Ettawa crossbred goats in this region, highlighting the potential for production losses from this overlooked disease.
Optimalisasi Limbah Daun Cengkeh Kering (Syzygium aromaticum) sebagai Repelan Alami Ramah Lingkungan: Optimization of Dried Clove Leaf Waste (Syzygium aromaticum) as an Environmentally Friendly Natural Repellant Putra, Zidhandika Nagita; Nugraheni, Yudhi Ratna; Prastowo, Joko; Ariyadi, Bambang; Awaludin, Aan
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 2 (2025): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v10i2.8435

Abstract

Munggang Wetan hamlet is one of the clove-producing areas in Kulon Progo, located in the Menoreh mountain plateau. These areas have the potential to spread malaria. The clove plant has insecticidal compounds that can be used as a mosquito repellent. Dried clove leaves are still considered waste that has not been utilized by the community. The purpose of this community service is to transfer knowledge to the people of Munggang Wetan hamlet in utilizing clove leaf waste as a natural mosquito repellent. The method used was counseling and practical. The extraction process of clove leaf waste for repellant uses the maceration method and utilizes 96% Ethanol as a solvent. The result of this activity was that the training participants were able to understand and make mosquito repellant by utilizing clove leaf waste and packaging it in spray bottles. Natural mosquito repellant derived from the extraction of clove leaf waste with 96% ethanol solvent can be used by the community and has effective work as a natural mosquito repellent.
Application of Buck Rotation System to Improve the Quality of Goat Phenotypes and Genotypes in KUBE X Sejahtera 005 Farmer Group Nugraheni, Yudhi Ratna; Prastowo, Joko; Ariyadi, Bambang; Awaludin, Aan; Muhamad, Nur
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 3 (2025): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v10i3.8478

Abstract

Goats are the leading livestock commodity in the Samigaluh sub-district. The goat population in the Samigaluh sub-district was 15,866 goats (16.7%) of the total goat population in Kulon Progo which reached 95,257 goats. Peranakan Ettawa (PE) goats are widely kept and bred because of their high quality and price value, some are crossbred with local goats called Bligon. Limited access and quality bucks are one of the obstacles in breeding and improving the quality of goats, the use of artificial insemination (AI) in goat breeding has a low success rate. This community service (PkM) aims to educate and foster awareness and the active role of farmers in maintaining and improving the quality of goats from the reproduction side. The PkM partner is the KUBE Sejahtera X Binangun 005 livestock group which is a productive community group within the scope of the UGM assisted village in Sidoharjo village. Transfer of knowledge and skills has been carried out using counseling methods, interactive discussions, and practice in selecting superior males and applying the Buck Rotation System to maintain and improve the quality of goats phenotypically and genotypically. Evaluation of PkM by taking the parameters of knowledge and skills of farmers in assessing and selecting superior bucks and recording reproduction by implementing the Buck Rotation System. Results achieved, the training participants of the KUBE Sejahtera X Binangun 005 farmer group understand the importance of reproductive health, reproductive recording, and the impact of inbreeding and can judge and select bucks by SNI 7352:2008.
Co-Authors . Dwilantiani . Rianawati Alek Arisona Alfarisa Nururrozi Ambar Pertiwiningrum Ana Sahara Ana Sahara April Hari Wardhana Argamjav, Bayanzul Arisona, Alek Asmarani Kusumawati Awaludin, Aan Bambang Ariyadi Bambang Ariyadi, Bambang Bambang Sutrisno Budi Purwo Widiarso Budi Purwo Widiarso BUDI SETIADI DARYONO Catur Sugiyanto Dwi Priyowidodo Dwi Priyowidodo Dyah Nur Hidayati Eryl Sri Rohayati Galuh Tresnani Herawati, Okti I Gusti Komang Oka Wirawan I Gusti Komang Oka Wirawan, I Gusti Komang Imron Rosyadi Kurniasih . Kurniasih . Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Lily Gunawan Margaretha Arnita Wuri Mubarokah, Wida Wahidah Mufasirin MUHAMAD, NUR Muhammad Hanafiah Nor Wijaya, Rendra Nugraheni, Yudhi Ratna Nugraheni, Yudhi Ratna Nurcahyo, R Wisnu Priyo Sambodo Priyowidodo P Priyowidodo P, Priyowidodo Putra, Zidhandika Nagita R. Wasito . Raden Wisnu Nurcahyo Ratna Nugraheni, Yudhi Rini Widayanti Sahara, Ana Sitarina Widyarini Soedarmanto Indarjulianto Soedarmanto Indarjulianto Sri Hartati Sri Hartati Sudarmanto Indarjulianto Sumartono Sumartono Sumartono Sumartono Sunandjak . Surya Widyarsi Teguh Ari Prabowo Wida Wahidah Mubarokah Wida Wahidah Mubarokah Widiarso, Widiarso, B. P. Windarti . Windarti Windarti Wisnu Nurcahyo Wisnu Nurcahyo Wisnu Nurcahyo Wisnu Nurcahyo Yanuartono Yanuartono . Yanuartono, Yanuartono Yosua Lede Pono