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IN VITRO DEVELOPMENT OF Ascaridia galli EGGS INTO INFECTIVE EGGS AND LARVAE OF STADIUM 2 (L2) Mubarokah, Wida Wahidah; Kurniasih, Kurniasih; Nurcahyo, Wisnu; Prastowo, Joko
Jurnal Kedokteran Hewan Vol 13, No 1 (2019): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i1.12978

Abstract

The study aimed at finding out the development of Ascaridia galli (A. gall) eggs that were given aerator treatment and those without aerator treatment into infective eggs and L2 through in vitro culture. Each treatments has 108,000 eggs assigned to 8 groups of 1,000; 2,000; 3,000; 4,000; 5,000; 6,000; 7,000; and 8,000 eggs, respectively with 3 repetitions. Female A. galli were collected from the small intestinal lumen of naturally infected domestic chickens. The eggs collected from the uterus of adult female A. galli were incubated in sterile aquadest at ambient temperature for 45 days (without aerator) and 25 days (with aerator) to obtain the infective eggs and the L2. The number of the infective eggs and hatched L2 were counted under stereo microscope. Data were analysed descriptively. There were 97.740 eggs (90.5%) in the groups without aerator developed into infective eggs and 77,040 eggs (71.3%) developed into the L2. Meanwhile, there were 101,847 eggs (94.3%) in the groups with the aerator developed into the infective eggs and88.722 eggs (82.15%) hatched L2. It is concluded that the eggs collected from worms uterus had high viability and the aerator application shortened the developing period of the A. galli worms.
MORPHOLOGY AND MORPHOMETRY OF Haemonchus contortus IN GOATS IN YOGYAKARTA, INDONESIA Sambodo, Priyo; Prastowo, Joko; Indarjulianto, Sudarmanto; Kurniasih, Kurniasih
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i3.11026

Abstract

This research was carried out to determine the morphology and morphometry of Haemonchus contortus in goats. Adult female parasites were collected from abomasum of naturally infected goats and stored in lactophenol. Images of the parasite were acquired using lucida camera and measurement was performed using Axiovision LE software. Basic characters of the morphology and morphometry of Haemonchus spp. were identified by determining the value of the discriminant function of three parameters on spicule. Vulvar morphology was identified by vulvar flap shape. Data were presented in terms of mean, standard deviation, and percentage. A hundred percent of the parasites were H. contortus with discriminant function value 0.63. The size of female parasites was longer and wider (25.5-32.6 mm; 0.38-0.63 mm) than that of male ones (17.3-20.0 mm; 0.24-0.33 mm). There were only 2 varieties of vulvar flap, linguiform (81%), and knobbed (19%). There were 5 subclasses identified in the linguiform type, including linguiform A (39%), linguiform B (22%), linguiform C (17%), linguiform I (1%), and a new subclass of linguiform D (2%). Based on the the type of vulvar flap, H. contortus found in goats in Yogyakarta were different to those found in previous researches. The findings could be used in identifying the parasite species in small ruminants.
THE EFFECT OF APUS BAMBOO (Gigantochloa apus) LEAVES INFUSION TO MORTALITY RATE AND MORPHOMETRY OF Haemonchus contortus ADULT WORM IN VITRO Widiarso, Budi Purwo; Nurcahyo, Wisnu; Kurniasih, Kurniasih; Prastowo, Joko
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.8167

Abstract

This present project was carried out to determine the effect of apus bamboo (Gigantochloaapus )leaves infusion to mortality rate and morphometry of Haemonchus contortus adult worms in vitro. The formulated tools were collected through camera, 3 ml syringe, object glass, deck glass, mortar, drip dropper, waterbath, microscope, stopwatch, counter check, oven, erlenmeyer flask, electric scale, surgical scissor, and pipette. The allocated materials used in this studywere bamboo leaves infusion, Haemonchus contortus adult worms, aquadestilata, ethanol and NaCl 0.62%. The research was firstly concernedwith making 0.1% and 1% apus bamboo leaves infusion. The samples were obtained from Haemonchus contortus collection. Adult worms were obtained directly from abomasum of naturally infected goats that were cut at Animal Slaughter House (RPH). To investigate the matter, mortality rates of adult worms at various doses and times of observation as well as differences in Haemonchus contortus morphometry were analyzed using ANOVA. The findings disclosed that apus bamboo leaves infusion demonstrated a significant difference in the mortality rate of adult worms Haemonchus contortusat various doses and times of observation. This report presented the findings of research that the best dose to increase the mortality of worms was 1% bamboo leaves infusion. Moreover, apus bamboo leaves infusion affected the morphometry of Haemonchus contortus adult worms, especially in body length, cervical papilla width, and spicules length in males, and body length, cervical papilla width, and vulvar length in females.
FAKTOR RISIKO INFEKSI Toxoplasma gondii PADA KUCING DOMESTIK YANG DIPELIHARA DI YOGYAKARTA Hanafiah, Muhammad; Nurcahyo, Wisnu; Prastowo, Joko; Hartati, Sri
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2792

Abstract

Tujuan penelitian adalah menentukan kemungkinan faktor-faktor risiko terhadap kemunculan toksoplasmosis. Sebanyak 132 sampel serum darah diambil pada kucing lokal di Yogyakarta. Data-data epidemiologis seperti asal usul kucing, ras kucing, jenis kelamin, umur, lokasi sistem pemeliharaan, jenis pakan, dan frekuensi diare diberi kode untuk mempermudah analisis, kemudian dimasukkan, disimpan, dan dianalisis dengan program Statistix Versi 7 (Analytical Sofware inc). Analisis data dilakukan secara bivariat (Chi-square (2), dan kekuatan asosiasi (OR), dan multivariat (regresi logistik). Hasil penelitian menunjukkan bahwa faktor-faktor yang berpengaruh terhadap adanya toksoplasmosis pada kucing berdasarkan analisis bivariat adalah pembersihan kotak pasir 1 kali sehari dan mandi 2-3 kali seminggu sedangkan faktor-faktor yang memiliki peluang meningkatkan seropositif toksoplasmosis berdasarkan analisis multivariat adalah pemeliharaan kucing yang bebas di dalam rumah, dimandikan lebih dari 1 kali seminggu, dan dimandikan lebih besar dari 1 bulan sekali.
IDENTIFIKASI TOKSOPLASMOSIS PADA FESES KUCING SECARA MIKROSKOPIS DAN SEROLOGIS Nurcahyo, Wisnu; Prastowo, Joko; P, Priyowidodo
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i2.2639

Abstract

Penelitian ini bertujuan mengetahui tingkat prevalensi Toxoplasma pada kucing dan menyusun basis epidemiologis pada kucing yang sangat penting dalam peranannya sebagai hospes definitif toksoplasmosis. Sampel serum darah dan feses kucing digunakan dalam penelitian ini. Metode pemeriksaan menggunakan card agglutination Toxoplasma test (CATT) Pastorex Toxo kit dan metode sentrifus. Data yang dip eroleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa tingkat prevalensi Toxoplasma dengan pemeriksaan serum darah kucing sebesar 6,8% dan pemeriksaan feses sebesar 9,4%. Infeksi toksoplasmosis yang terjadi pada kucing secara umum dari pemeriksaan klinis tidak mempunyai gejala yang spesifik.
Infestasi Ektoparasit pada Pasien Kucing yang Memiliki Masalah Kulit di Klinik Hewan Lilipoet Yogyakarta Gunawan, Lily; Indarjulianto, Soedarmanto; Yanuartono, Yanuartono; Nurcahyo, R Wisnu; Prastowo, Joko
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.75941

Abstract

Ektoparasit merupakan problem yang sering diderita kucing, termasuk di Yogyakarta. Penelitian ini bertujuan untuk mengetahui prevalensi infestasi ektoparasit pada kucing dan jenisnya di wilayah Klinik Hewan Lilipoet Yogyakarta. Penelitian ini dilakukan menggunakan 173 pasien kucing penderita dermatitis. Data yang diambil adalah umur, jenis kelamin dan diagnosis berdasarkan pemeriksaan kulit. Sampel ektoparasit diambil dari kucing dengan metode combing. Ektoparasit diidentifikasi secara mikroskopik dari sampel kulit. Hasil penelitian menunjukkan 120 dari 173 (69,3%) kucing yang diperiksa ditemukan ektoparasit. Spesies ektoparasit tersebut terdiri dari: Otodectes cynotis (30%), Ctenocephalides felis felis (25,8%), Notoedres cati (25%), Lynxacarus radovskyi (15,8%), Felicola subrostratus (2,5%), dan Rhipicephalus sanguineus (0,8%). Sebanyak 16 dari 120 (13,3%) pasien kucing menderita infestasi beberapa ektoparasit. Jumlah ektoparasit yang ditemukan pada kucing umur 1-12 bulan sebanyak 86/120 (71,6%), umur12-24 bulan sebanyak 13/120 (10,8%), dan pada umur lebih dari 24 bulan sebanyak 21/120 (17,5%). Ektoparasit ditemukan pada kucing jantan sebanyak 69/120(57,5%) dan pada betina 51/120 (42,5%). Disimpulkan bahwa prevalensi infestasi ektoparasit pada kucing penderita dermatitis di klinik Lilipoet Yogyakarta adalah 69,3%. Infestasi ektoparasit banyak ditemukan pada kucing berumur dibawah 1 tahun dan lebih sering ditemukan pada kucing jantan daripada betina.
Efektivitas Albendazole terhadap Nematodiasis pada Sapi Perah di Peternakan Sapi Perah Rakyat Kecamatan Tegalombo Kabupaten Pacitan Arisona, Alek; Prastowo, Joko; Priyowidodo, Dwi; Yanuartono, Yanuartono; Indarjulianto, Soedarmanto
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.79447

Abstract

Nematodiasis adalah penyakit yang disebabkan cacing nematoda yang dapat diderita sapi perah segala usia. Penyakit ini mempunyai gejala klinis antara lain kurus, rambut kusam berdiri dan diare. Albendazole merupakan obat cacing berspektrum luas yang sering digunakan oleh peternak karena mudah didapat. Tujuan penelitian ini adalah menentukan prevalensi, gejala klinis dan efektifitas albendazole terhadap nematodiasis pada sapi perah di peternakan sapi perah rakyat Kecamatan Tegalombo, Kabupaten Pacitan. Penelitian ini menggunakan 50 ekor sapi perah yang tidak bunting. Semua sapi diperiksa secara fisik meliputi kondisi umum sapi dan feses. Sampel feses diambil dan diperiksa adanya telur cacing dengan metode nativ, apung dan Mc Master. Sapi penderita nematodiasis selanjutnya diterapi albendazole dengan dosis 10 mg/kg BB satu kali pemberian secara per oral. Perkembangan hasil terapi diamati dengan cara dilakukan pemeriksaan fisik, pemeriksaan sampel feses pada hari ke 3, 7 dan 14. Hasil penelitian menunjukkan bahwa prevalensi nematodiasis sapi perah sebesar 30%. Sapi penderita nematodiasis menunjukkan feses lembek, adanya telur nematoda pada fesesnya. Telur cacing yang ditemukan adalah kelompok telur Strongyle, Toxocara sp, dan Trichuris sp. Efektivitas albendazole terhadap nematodiasis pada sapi perah masih tinggi yakni >97,87%. Disimpulkan bahwa albendazole mempunyai efektifitas yang tinggi untuk mengobati nematodiasis.
Empowering Farmers through Fine Crumble Organic Fertilizer Innovation for Sustainable and Eco-Friendly Agriculture Ariyadi, Bambang; Pastawan, Viagian; Prastowo, Joko; Nugraheni, Yudhi Ratna; Mubarokah, Wida Wahidah; Utomo, Budi; Awaludin, Aan
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 11 No. 2 (2026): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v11i2.11097

Abstract

Munggang Wetan Hamlet, Sidoharjo Village, Samigaluh District, Kulon Progo, is in the Menoreh Highhills region, which has great goat farming potential. The KUBE Sejahtera X Binangun 005 farm group has been using goat manure as organic fertilizer; however, they continue to encounter challenges due to its rough texture and basic packaging. This community service activity aims to improve the quality of organic fertilizer products by applying Compost Maturation Technology and using a compost grinder to produce fine, crumbly organic fertilizer. The activity was conducted using a participatory approach that included outreach, training, technology application, and evaluation. The results of this community service activity showed a significant improvement in the physical quality of organic fertilizer that meets SNI standards, with characteristics such as a crumbly texture, blackish-brown color, a humus-like odor, and a temperature of 30–35°C, making it suitable for agricultural use. Additionally, the innovative packaging design has the potential to enhance the sales value of the partner group's organic fertilizer products. This activity not only supports local economic potential but also reinforces the principles of sustainable agriculture by utilizing livestock manure as a productive resource. This program is expected to become a model for rural community empowerment through appropriate, environmentally friendly technology.
Uncovering subclinical diseases in sacrificial livestock in Kulon Progo, Indonesia: A comparative approach using ante-mortem and post-mortem inspections Awaludin, Aan; Nugraheni, Yudhi Ratna; Arnuphapprasert, Apinya; Wibowo, Sarwo Edy; Widiarso, Budi Purwo; Prastowo, Joko; Nurcahyo, Raden Wisnu; Andityas, Morsid; Muhamad, Nur; Nurfitriani, Rizki Amalia
Jurnal Ilmu Peternakan Terapan Vol 9 No 2 (2026): Jurnal Ilmu Peternakan Terapan
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/jipt.v9i2.6914

Abstract

Some cases of animal diseases are difficult to detect clinically, especially chronic and subclinical diseases. The condition of subclinical diseases poses the potential for hidden disease spread within a livestock population. This research aims to uncover subclinical diseases occurring in sacrificial livestock. The methodology employed involved a combination of ante-mortem and post-mortem examinations performed on the same individual animals. The study was conducted in Bunder Tengah, located within the Progo River watershed area. The subjects of the study consisted of four male cattle, eight male Etawa Crossbred goats (ECG), and one ram. The results indicated that during the ante-mortem examination, all animals were diagnosed as clinically healthy; however, the post-mortem examination revealed that two cattle (ID: BC-1 and BC-4) and one goat (ID: G-4) were infected with fasciolosis. The conclusion of this study is the combination of ante-mortem and post-mortem inspections can be used to uncover clinically hidden diseases, allowing information about disease occurrences in a region to be continuously monitored and identified, which can then serve as a guideline in planning disease prevention programs. Laboratory examinations as part of ante-mortem inspections should be conducted to obtain early information about disease occurrences in a particular area.
Co-Authors . Dwilantiani . Rianawati Alek Arisona Alfarisa Nururrozi Ambar Pertiwiningrum Ana Sahara Ana Sahara April Hari Wardhana Argamjav, Bayanzul Arisona, Alek Arnuphapprasert, Apinya Asmarani Kusumawati Awaludin, Aan Bambang Ariyadi Bambang Ariyadi, Bambang Bambang Sutrisno Budi Purwo Widiarso Budi Purwo Widiarso BUDI SETIADI DARYONO Budi Utomo Catur Sugiyanto Dwi Priyowidodo Dwi Priyowidodo Dyah Nur Hidayati Eryl Sri Rohayati Galuh Tresnani Herawati, Okti I Gusti Komang Oka Wirawan I Gusti Komang Oka Wirawan, I Gusti Komang Imron Rosyadi Kurniasih . Kurniasih . Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Lily Gunawan Margaretha Arnita Wuri Morsid Andityas Mubarokah, Wida Wahidah Mufasirin MUHAMAD, NUR Muhammad Hanafiah Nor Wijaya, Rendra Nugraheni, Yudhi Ratna Nugraheni, Yudhi Ratna Nurcahyo, R Wisnu Nurcahyo, Raden Wisnu Pastawan, Viagian Priyo Sambodo Priyowidodo P Priyowidodo P, Priyowidodo Putra, Zidhandika Nagita R. Wasito . Raden Wisnu Nurcahyo Ratna Nugraheni, Yudhi Rini Widayanti Rizki Amalia Nurfitriani Sahara, Ana Sarwo Edy Wibowo Sitarina Widyarini Soedarmanto Indarjulianto Soedarmanto Indarjulianto Sri Hartati Sri Hartati Sudarmanto Indarjulianto Sumartono Sumartono Sumartono Sumartono Sunandjak . Surya Widyarsi Teguh Ari Prabowo Wida Wahidah Mubarokah Wida Wahidah Mubarokah Widiarso, Budi Purwo Widiarso, Widiarso, B. P. Windarti . Windarti Windarti Wisnu Nurcahyo Wisnu Nurcahyo Wisnu Nurcahyo Wisnu Nurcahyo Yanuartono Yanuartono . Yanuartono, Yanuartono Yosua Lede Pono