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PEMURNIAN ENZIM SEFALOSPORIN-C ASILASE DAN OPTIMASI PROSES KROMATOGRAFI PENUKAR ION Nasution, Uli Julia; Wijaya, Silvia Melinda; Wibisana, Ahmad; Safarrida, Anna; Rachmawati, Indra; Puspitasari, Dian Japany; Naim, Sidrotun; Mahsunah, Anis Herliyanti; Wulyoadi, Sasmito; Suyanto, Suyanto
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 2 (2018): December 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Purification of Cephalosporin-C Acylase and Its Optimization of Ion-Exchange ChromatographyABSTRACTCephalosporin-C acylase (CCA) has an important role in the one-step conversion of cephalosporin-C into 7-ACA. Purification process aims to increase specific activity of CCA enzyme. Purification began with cell lysis, ammonium sulphate precipitation, dialysis, ion exchange chromatography (IEC) and size exclusion chromatography. IEC optimization of elution step was also done to compare gradient and isocratic elusion. Purification was capable to increase the enzyme purity upto 33.66 fold, with specific activity of 3.00 U/mg and the yield reached 41.41%. Optimization of elusion during IEC showed that isocratic protein elusion was more efficient (taking shorter time, 3 column volume (CV) only) than that of gradient batch (up to 9 CV). SDS-PAGE analysis demonstrated that the recombinant CCA enzyme existed in two types, active enzyme containing ÃŽÂ±-subunit (25 kDa) and ÃŽÂ²-subunit (58 kDa), and inactive enzyme (83 kDa) as precursor. Furthermore, 30% ammonium sulphate saturated precipitation was able to precipitate this inactive CCA.Keywords: 7-ACA,CCA, cephalosporin C, protein purification, specific activityÃ‚Â ABSTRAKSefalosporin-C asilase (CCA) merupakan enzim yang berperan penting dalam konversi satu tahap sefalosporin-C menjadi 7-ACA. Proses purifikasi merupakan salah satu cara untuk meningkatkan aktivitas spesifik enzim CCA. Proses purifikasi dimulai dari memecah sel, diikuti dengan tahap presipitasi menggunakan amonium sulfat, dialisis, kromatografi penukar ion (IEC) dan kromatografi eksklusi. Optimasi proses IEC pada tahap elusi juga dilakukan untuk membandingkan elusi enzim CCA secara gradien dan isokratik. Proses purifikasi pada penelitian ini mampu meningkatkan kemurnian enzim hingga 33,66 kali, dengan aktivitas spesifik sebesar 3,00 U/mg dan perolehan enzim sebesar 41,41%. Hasil optimasi IEC pada proses elusi secara isokratik lebih efisien dari segi waktu (hanya membutuhkan 3 kolom volume (CV) dibandingkan dengan secara gradien (sampai 9 CV). Hasil SDS-PAGE menunjukkan bahwa CCA rekombinan merupakan enzim dengan 2 macam bentuk yaitu enzim aktif, yang terdiri dari subunit ÃŽÂ± (25 kDa) dan ÃŽÂ² (58 kDa), dan enzim tidak aktif berupa prekursor (83 kDa). Proses presipitasi menggunakan amonium sulfat 30% tersaturasi dapat mengendapkan prekursor CCA.Kata Kunci: 7-ACA, aktivitas spesifik, CCA, purifikasi protein, sefalosporin C

INFLUENCE OF WORK ENVIRONMENT AND EMPLOYEE COMPETENCE ANALYSIS ON EMPLOYEE PERFORMANCE Hery Santoso, Muhammad; Naim, Sidrotun; Suroso, Suroso; Hayudini, Magna Anissa A.; Shrestha, Prabin
Chanos Chanos Vol 21, No 1 (2023): CHANOS CHANOS
Publisher : Politeknik Kelautan dan Perikanan Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/chanos.v21i1.12818

The research that has been done aims to analyze the effect of the work environment and employee competence on employee performance at a seafood processing company in Probolinggo City, East Java. Based on the results of the analysis of the calculation of the first hypothesis (H1), which states that the work environment has a positive and significant effect on employee performance, the t-count value of 19,845 is greater than the t-table value, which is 1,677 and the significance value obtained is less than 0.05, which means that the work environment has a positive and significant effect on employee performance, the results of the calculation analysis of the second hypothesis (H2) which states that employee competence has a positive and significant effect on employee performance obtained a t-count value of 14,471 greater than the t-table value of 1,677 and the value the significance obtained is less than 0.05, which means that employee competence has a positive and significant effect on employee performance, while the R-square value obtained is 0.897 which states that work environment variables and employee competency affect employee performance by 89.70% and the remaining 10.30% influenced by other variables.

Exploring the Relationship Between Leadership Styles and Employee Motivation in the Context of Contemporary Management Djoko Soelistya; Naim, Sidrotun; Muliyati, Muliyati; Anshoriyah, Sofiyatul; Syafri, Muhammad
International Journal of Economics (IJEC) Vol. 3 No. 2 (2024): July-December
Publisher : PT Inovasi Pratama Internasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55299/ijec.v3i2.1098

The objective of this research is to examine the relationship between leadership styles and employee motivation in the context of contemporary management. This research employs a literature review approach to analyse various leadership theories, including transformational, transactional, autocratic, democratic, laissez-faire, and servant leadership. Each leadership style is evaluated in relation to employees' intrinsic and extrinsic motivation. The findings indicate that transformational and democratic leadership styles have the most significant influence on employees' intrinsic motivation, whereas transactional and autocratic leadership styles emphasize extrinsic motivation. This study contributes to contemporary management literature by providing insights into the importance of tailoring leadership styles to employees' motivational needs to achieve optimal performance.

STRATEGI KOMUNIKASI POLITIK MARUF AMIN DALAM KAMPANYE PEMILU 2019: PEMBELAJARAN UNTUK POLITIK ANTI-HOAX Widjayanto, Febby R; Naim, Sidrotun
Paradigma POLISTAAT: Jurnal Ilmu Sosial dan Ilmu Politik Vol. 5 No. 1 (2022): Paradigma POLISTAAT: Jurnal Ilmu Sosial dan Ilmu Politik
Publisher : Fakultas Ilmu Sosial dan Ilmu Politik Universitas Pasundan Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/paradigmapolistaat.v5i1.5866

Social media feed—which is populated with text embedded in numerous forms of multimedia contents—has been a crucial battleground for politicians to situate their policy relevance to the public during election campaign. Narrative building is somewhat played out by politicians in digital space to seek an earlier assurance of their victory. This article analyses how the proponent 2019 Indonesia presidential election candidate, Joko Widodo and his running mate, Ma’ruf Amin assigns a duality of function to the anti-hoax narrative besides renders it as a firm stance they take to differ from their rival. We employ action research at the scene drawing upon the five-month period of observing one of the consultative groups to the candidate on the matter of digital campaign, and at times sharing our perspective on digital literacy and tackling disinformation. Anti-hoax narrative in the context of election turns out to be an inescapable hot topic that arguably serves as a sort of ‘free-to-use political resource’ ready to be availed of by politicians to reiterate an agenda, counterbalance and issue, or even instill a new idea regarding nationhood.

ADAPTIVE LEADERSHIP IN THE ERA OF DISRUPTION: MANAGING TEAM WITH FLEXIBILITY Naim, Sidrotun
INTERNATIONAL JOURNAL OF SOCIETY REVIEWS Vol. 2 No. 8 (2024): AUGUST
Publisher : Adisam Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar

Era of disruption demand every leader For manage his team with flexibility, through application leadership adaptive. Study This aim For identify and analyze problem in apply leadership adaptive and defining effective strategy in management team in environment dynamic organization. Through study literature, analysis show that leadership adaptive need approach holistic and adaptive that pays attention dynamics psychological individual, ability analytical, interpersonal skills, management source dynamic power, as well development capacity inclusive leadership. Research result recommend necessity investment in training, development, and support for leader as well as member team For overcome challenge and exploit opportunity. Strategy main covers define vision, recognize uniqueness team, building environment learn, adapt to change, communication two direction, as well leadership share. Study This expected can give outlook in formulate strategy leadership Effective adaptation in an era of disruption.

Strategic Convergence: How Knowledge Sharing, Cross-Functional Collaboration, and Adaptive Leadership Drive Innovation Success Tobari, Tobari; Dewi, Maftuhah; Naim, Sidrotun; Nur Azizah, Siti
International Journal of Business, Law, and Education Vol. 5 No. 2 (2024): International Journal of Business, Law, and Education
Publisher : IJBLE Scientific Publications Community Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56442/ijble.v5i2.888

This study investigates the relationships among knowledge sharing, cross-functional collaboration, adaptive leadership, and innovation success in organizational settings. Employing Partial Least Squares Structural Equation Modeling (PLS-SEM), the research analyzes data collected from employees across various industries. The findings reveal that knowledge sharing significantly influences innovation success both directly and indirectly through cross-functional collaboration, which also serves as a critical mediator between adaptive leadership and innovation outcomes. Furthermore, adaptive leadership is shown to enhance cross-functional collaboration, emphasizing the importance of leadership style in fostering a collaborative culture conducive to innovation. These results underscore the necessity for organizations to cultivate knowledge-sharing practices, promote cross-functional teamwork, and develop adaptive leadership capabilities to drive innovation success. The study contributes to the literature by elucidating the dynamic interplay among these constructs and providing practical implications for managers seeking to enhance their organization's innovative capacities.

How Entrepreneurial Mindset, Gender Stereotypes, and Innovation Practices Influence the Sustainability of Women-Owned Businesses in Bogor City, Indonesia Hakim, Nur; Ode, Haruni; Rahmadhani, May Vitha; Naim, Sidrotun
International Journal of Business, Law, and Education Vol. 5 No. 2 (2024): International Journal of Business, Law, and Education
Publisher : IJBLE Scientific Publications Community Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56442/ijble.v5i2.891

This study investigates the influence of entrepreneurial mindset, gender stereotypes, and innovation practices on the sustainability of women-owned businesses in Bogor City, Indonesia. Using a sample of 200 women entrepreneurs, data was collected through structured questionnaires and analyzed using multiple regression analysis. The results indicate that entrepreneurial mindset and innovation practices positively impact business sustainability, while gender stereotypes have a negative effect. The findings emphasize the critical role of fostering a strong entrepreneurial mindset and encouraging innovation among women entrepreneurs, while also addressing the detrimental impact of gender stereotypes on business operations. The study contributes to the growing body of literature on women’s entrepreneurship and offers practical insights for policymakers and support organizations in creating a conducive environment for the sustainability of women-owned businesses

Analysis Of The Effect Of Job Satisfaction And Tenure On Turnover Intention Soeprapto, Adi; Tawil, Muhammad Risal; Naim, Sidrotun; Buamonabot, Irfandi; Thahrim, Muhammad
Jurnal Ekonomi Vol. 13 No. 03 (2024): Jurnal Ekonomi, Edition July -September 2024
Publisher : SEAN Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar

This study aims to analyze the effect of job satisfaction and tenure on turnover intention. Using the survey method, data was collected from 30 employees from various industry sectors. Pearson correlation analysis showed that there is a significant negative relationship between job satisfaction and turnover intention (r = -0.527, p < 0.05), and between tenure and turnover intention (r = -0.382, p < 0.05). Multiple regression analysis shows that job satisfaction and tenure significantly affect turnover intention (p < 0.05), with a negative coefficient indicating that an increase in job satisfaction and tenure will decrease turnover intention. The interaction analysis shows that there is a significant interaction between job satisfaction and tenure in influencing turnover intention (p < 0.05). The results of this study provide insights for human resource managers to increase job satisfaction and retain long-term employees to reduce turnover intention. With the right strategies, organizations can improve employee retention and overall productivity.

Exploring the Relationship Between Leadership Styles and Employee Motivation in the Context of Contemporary Management Djoko Soelistya; Naim, Sidrotun; Muliyati, Muliyati; Anshoriyah, Sofiyatul; Syafri, Muhammad
International Journal of Economics (IJEC) Vol. 3 No. 2 (2024): July-December
Publisher : PT Inovasi Pratama Internasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55299/ijec.v3i2.1098

The objective of this research is to examine the relationship between leadership styles and employee motivation in the context of contemporary management. This research employs a literature review approach to analyse various leadership theories, including transformational, transactional, autocratic, democratic, laissez-faire, and servant leadership. Each leadership style is evaluated in relation to employees' intrinsic and extrinsic motivation. The findings indicate that transformational and democratic leadership styles have the most significant influence on employees' intrinsic motivation, whereas transactional and autocratic leadership styles emphasize extrinsic motivation. This study contributes to contemporary management literature by providing insights into the importance of tailoring leadership styles to employees' motivational needs to achieve optimal performance.

The Effect of Financial Management Quality, Product Innovation, and Digital Marketing on Micro Business Growth in the Jakarta Culinary Sector Naim, Sidrotun
West Science Business and Management Vol. 2 No. 01 (2024): West Science Business and Management
Publisher : Westscience Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58812/wsbm.v2i01.734

This research investigates the dynamics of micro-business growth in the Jakarta Culinary Sector, focusing on the impact of Financial Management Quality (FMQ), Product Innovation (PI), and Digital Marketing (DM). A quantitative analysis involving a sample of 150 micro businesses reveals compelling insights. Results indicate significant positive relationships between FMQ, PI, and DM with Micro Business Growth (MBG). The study emphasizes the pivotal role of sound financial practices, innovation, and digital marketing in fostering growth within the competitive culinary landscape of Jakarta. Findings contribute to the understanding of success factors in micro-businesses, offering practical implications for entrepreneurs and policymakers.

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