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Phenotypic and Genotypic Detection of Staphylococcus aureus and Escherichia coli in Subclinical Mastitis Goat Milk in Lumajang, Indonesia Khasanah, Himmatul; Putra, Nalendra Gigih Wibawanto; Hariyadi, Nanang Tri; Widodo, Nur; Widianingrum, Desy; Yulianto, Roni; Cruz, Joseph F. Dela; Pratiwi, Nurul
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.167-174

Abstract

This study aimed to identify the Staphylococcus aureus and Escherichia coli bacteria that cause subclinical mastitis in dairy goats in the Senduro sub-district of Lumajang regency, Indonesia. A total of 116 milk samples from 58 dairy goats were tested for the California Mastitis Test (CMT). CMT-positive samples were used for bacterial identification through phenotypic and genotypic analyses. Phenotypic analysis was conducted by culturing bacteria on natrium agar media and followed in selective media. The genotypic analysis employed a specific primer of S. aureus using the 23SrRNA gene and E. coli using the 16SrRNA gene as confirmation of the gold standart. The results of this study showed positive subclinical mastitis in dairy goat samples, which consisted of 21 samples of CMT 1 and a sample of CMT 2. Bacterial identification with phenotypic assays documented 16 samples having S. aureus and one sample having E. coli. The validated genotypic assays showed from 16 samples, validated 5 samples of S. aureus infection and one E. coli infection. In conclusion, bacterial identification of S. aureus and E. coli predominantly infects the SCM dairy goats in the Senduro sub-district as evidenced by the phenotypic analysis and followed by genotypic using the PCR method.
Pengembangan Proklim Melalui Pemanfaatan Pekarangan Berkelanjutan di Desa Binaan Wonoasri, Kecamatan Tempurejo, Kabupaten Jember Sulistiyowati, Hari; Hasanah, Ela Apriliyanti; Ratnasari, Tri; Dewi, Nilasari; Siddiq, Arif Mohammad; Kurnianto, Agung Sih; Yulianto, Roni; Widodo, Nur
Reswara: Jurnal Pengabdian Kepada Masyarakat Vol 5, No 2 (2024)
Publisher : Universitas Dharmawangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46576/rjpkm.v5i2.4112

Abstract

Desa Wonoasri memiliki potensi dalam pengembangan program kampung Iklim (ProKlim) yang salah satunya pada aspek pemanfaatan pekarangan masyarakat. Oleh karena itu kegiatan pengabdian ini bertujuan untuk mengembangkan Desa ProKlim melalui pemanfaatan pekarangan berkelanjutan di Desa binaan Wonoasri, Kecamatan Tempurejo, Kabupaten Jember. Metode pendekatan yang digunakan adalah pendekatan personal dan kelompok melalui sosialisasi dan studi banding, Focus Group Discussion (FGD), dan pendampingan serta demonstrasi. Tolok ukur keberhasilan pengembangan Proklim adalah respon positif Masyarakat dan perangkat desa terhadap keseluruhan kegiatan yang akan dilakukan. Hasil dari kegiatan pengabdian ini adalah respon positif dari masyarakat terhadap ProKlim yang cukup tinggi dengan keterlibatan 35 masyarakat yang mewakili berbagai kalangan (anak muda, ibu-ibu PKK, bapak-bapak, LMDHK, perangkat desa) dan diskusi interaktif selama kegiatan. Selain itu tiga kolam ikan lele model terpal berkerangka besi diameter 3m dengan masing-masing 1000 bibit ikan lele telah direalisasikan. Demikian juga halnya dengan realisasi 6 rangka besi vertikultur tanaman sayuran yang meliputi tomat, terong, dan pakcoi. Aktivitas diversifikasi produk mangga juga sudah dilaksanakan yaitu memberikan teori dan praktik dalam diversifikasi olahan buah mangga menjadi sirup, puding, kue bolu, dan manisan. Kesimpulan dari kegiatan pengabdian ini yaitu pengembangan ProKlim di Desa Wonoasri berhasil dilakukan berdasarkan respon positif masyarakat, realisasi kolam ikan dan vertikultur, dan diversifikasi produk buah mangga. Selanjutnya program ini juga mendapat dukungan sepenuhnya dari Kepala Desa Wonoasri yang akan melanjutkan program Desa ProKlim.
Pendampingan pengolahan pakan melalui teknologi Solid State Fermentation limbah kulit kopi di kelompok ternak sapi di Kabupaten Bondowoso, Jawa Timur Khasanah, Himmatul; Widianingrum, Desy Cahya; Widodo, Nur; Yulianto, Roni; Kusbianto, Dwi Erwin; Purnamasari, Listya; Fajrin, Eistifani; Isnaeni, Purnaning Dhian; Suryandari, Putrika
SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Vol 9, No 3 (2025): May
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jpmb.v9i3.30609

Abstract

Abstrak Permasalahan pakan menjadi salah satu kendala yang dialami oleh masyarakat peternak sapi potong di kabupaten Bondowoso, Jawa Timur. Sistem pengoleksian pakan cut and carry sulit dilakukan pada musim kemarau. Sebagian besar lahan pertanian di kabupaten Bondowoso merupakan lahan Perkebunan kopi. Limbah kulit kopi yang dihasilkan dari industry kopi di kabupaten Bondowoso masih kurang optimal. Kulit kopi memiliki kandungan nutrient yang masih potensial untuk dapat digunakan sebagai pakan ternak. Keberadaan antinutrisi dan rendahnya kecernaan menjadi faktor pembatas dalam pemanfaatannya sebagai pakan ternak. Pengabdian ini bertujuan untuk meningkatkan keterampilan peternak dalam menyediakan pakan ternak sapi potong yang bernilai gizi tinggi asal limbah kulit kopi melalui teknologi solid state fermentation. Kegiatan diawali dengan sosialisasi mengenai pakan komplit untuk ternak sapi potong, pelatihan pengolahan pakan asal kulit kopi, dan monitoring pemberian pakan pada ternak. Hasik kegiatan pengabdian menunjukan bahwa Masyarakat peternak sangat antusias mengikuti kegiatan baik sosialisasi, pelatihan maupun monitoring. Ternak sapi potong yang diberikan pakan komplit asal limbah kulit kopi melalui teknologi SSF selama 4 bulan juga memiliki pertambahan bobot badan yang lebih tinggi yaitu 144 kg dibandingkan ternak sapi yang hanya diberi limbah kulit kopi tanpa diolah yaitu 120 kg. Kata kunci: Bondowoso; sapi; pakan; fermentasi; kulit kopi Abstract The issue of feed availability is one of the major challenges faced by the beef cattle farming community in Bondowoso District, East Java. The cut-and-carry feed collection system becomes particularly difficult during the dry season. Most of the agricultural land in Bondowoso District is used for coffee plantations. However, the utilization of coffee husk waste produced by the local coffee industry remains suboptimal. Coffee husks contain nutrients that have the potential to be used as animal feed. Nevertheless, the presence of antinutritional factors and low digestibility limit their effectiveness as feed. This community service initiative aims to enhance farmers' skills in providing nutritionally rich beef cattle feed by utilizing coffee husk waste through solid-state fermentation technology. The program began with a socialization session on complete feed for beef cattle, followed by training on how to process feed from coffee husks, and concluded with monitoring the implementation of feed usage for livestock. The results of the service activities show that the farming community was highly enthusiastic about participating in all aspects of the program, including socialization, training, and monitoring. Beef cattle that were fed complete feed made from coffee husk waste processed using solid-state fermentation (SSF) technology for four months showed a higher body weight gain of 144 kg, compared to those fed unprocessed coffee husk waste, which gained only 120 kg. Keywords: Bondowoso; cattle; feed; fermentation; coffee husk.
Pemberdayaan Petani melalui Produksi Mikroorganisme Lokal (MOL) dan Plant Growth Promoting Rhizobacteria (PGPR) untuk Meningkatkan Produktivitas Padi dan Silase di Desa Kemiri, Kabupaten Jember Tanzil, Ahmad Ilham; Muhlison, Wildan; Saputra, Tri Wahyu; Rahayu, Puji; Yulianto, Roni; Anantoro, Tri; Hadi, Yusnan; Alfarisy, Fariz Kustiawan; Pusparani, Syafina; Rachmandhika, Yusuf; Syahputra, Wahyu Nurkholis Hadi
Jurnal ABDINUS : Jurnal Pengabdian Nusantara Vol 9 No 3 (2025): Volume 9 Nomor 3 Tahun 2025
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/ja.v9i3.23940

Abstract

Kemiri Village, Panti District, Jember Regency is one of the rice-producing villages but faces the problem of scarcity of subsidized fertilizers and ineffective management of agricultural waste. This needs to be overcome with simple but effective handling. This community service activity aims to empower farmers through the production of Local Microorganisms (MOL) and Plant Growth Promoting Rhizobacteria (PGPR) to increase rice productivity while supporting the potential of livestock in the village. The target partner in this activity is the Sidodadi farmer group consisting of 30 farmers. The implementation method includes training in making MOL and PGPR, applying PGPR to rice plants, and making silage as animal feed. The results of the activity show that the activities carried out related to the use of PGPR can help increase rice growth, improve yields, and reduce dependence on chemical fertilizers. In addition, making silage successfully processes green fodder into quality feed that supports food security for livestock in the village. In the future, this activity is expected to strengthen the sustainability of agricultural and livestock practices in Kemiri Village and improve the economy of farmers.
TERNAK SEHAT DENGAN PAKAN FERMENTASI DARI LIMBAH PERTANIAN SEBAGAI UPAYA KETAHANAN PANGAN Tirmidzi, Farkhan Rama; Saltsabita Nourin Adelina; Agustin, Luly Alfiana; Envizha, Adiza Zhafrana; Baihaqi, Mohammad Alfan; Muhammad, Adibillah; Syafira, Rifda Nadia; Meisari, Intan; Septianingtyas, Tri Pangesti; Umniyyah, Zahratul; Yulianto, Roni; Khasanah, Nisaul
Mafaza : Jurnal Pengabdian Masyarakat Vol. 4 No. 1 (2024): Mafaza : Jurnal Pengabdian Masyarakat
Publisher : Mafaza : Jurnal Pengabdian Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32665/mafaza.v4i1.2944

Abstract

The role of Sidomukti Village as an agricultural and livestock center in Mayang District, Jember Regency creates obstacles, namely the lack of water when the dry season arrives, as a result of which farmers find it difficult to grow crops. This also has an impact on village farmers who use agricultural waste as animal feed. On the other hand, in the rainy season, the availability of agricultural waste as animal feed is very abundant, so it is necessary to utilize agricultural waste into alternative feed that has a longer shelf life through a fermentation process. The aim of empowering the community in Sidomukti Village through processing agricultural waste is to make fermented feed an alternative for breeders when the amount of feed is limited because of its long shelf life and increasing the appetite of livestock so that livestock become healthy. The partners of this program are breeders and farmers of Sidomukti Village through outreach and demonstration activities. The methods and stages carried out in this service activity include location observation, activity planning, outreach, practice/demonstration, and evaluation. There were 34 farmers as well as breeders and prospective breeders as participants in the socialization and demonstration. The fermented rice straw produced has a characteristic brownish color, a typical sour fermented aroma, and a slightly crumbly texture. Healthy livestock after providing fermented feed is one of the advantages of this program, namely not only as an alternative to the limited availability of animal feed, but also as an effort to maintain national food security.