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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Pengabdian Masyarakat Peternakan JURNAL KAJIAN VETERINER Jurnal Media Tropika: Jurnal Pengabdian Kepada Masyarakat Jurnal Veteriner Nusantara
Sitompul, Yeremia Yobelanno
Universitas Nusa Cendana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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DESAIN PRIMER BERDASARKAN GEN MT-12S rRNA UNTUK MENDETEKSI CEMARAN DAGING BABI PADA PRODUK OLAHAN ASAL DAGING SAPI DENGAN METODE MULTIPLEKS-PCR Sitompul, Yeremia Yobelanno; Artanto, Sidna
Jurnal Sain Veteriner Vol 43, No 1 (2025): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.53197

Abstract

The case of adulteration of pork in beef products, which causes fretfulness especially for muslims, is still found in Indonesia. Multiplex Polymerase Chain Reaction (Multiplex PCR) can help detecting the adulteration of pork in beef products. This method needs multiple specific primers to amplify several targeted DNA mixture in single step reaction. This study aimed to design specific primers that can be utilized to detect the adulteration of pork in beef products using Multiplex PCR method.Firstly, primers were designed in silico using bioinformatic tools which were MegAlign, Seqbuilder, and AmplifX. This design was based on mt-12s rRNA gene. The length of amplified fragment using the designed primers for cow was 91 bp, while the pig was 230 bp. Secondly, the designed primers were tested to amplify DNA of cow and pig from raw beef, pork, mixture of beef and pork, and twelve samples of their products (meatballs) to assess their ability to amplify targeted DNA spesifically.The result showed that the designed primers could amplify the targeted DNA specifically from raw beef, pork, mixture of both meat, and their products. Therefore, the designed primers evidently can be used to detect adulteration of pork in beef by Multiplex PCR method.
MEMBANGUN KESIAPSIAGAAN KESEHATAN HEWAN BERBASIS ONE HEALTH: PELATIHAN PENGEMBANGAN PROFESIONAL BAGI DOKTER HEWAN GARIS DEPAN DI PULAU TIMOR, ROTE, DAN SABU Gelolodo, Maria Aega; Simarmata, Yohanes Timbun Raja Mangihut Ronael; Kallau, Novalino Harold Geoffrey; Tabali, Zulkifli; Sitompul, Yeremia Yobelanno; Loe, Fhady Risckhy; Wuhan, Yustinus Oswin Primajuni
Jurnal Media Tropika Vol 5 No 2 (2025): Jurnal Media Tropika
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/mediatropika.v5i2.25844

Abstract

Pengaruh Kombinasi Anestesi Ketamine-Medetomidine dengan Premedikasi Acepromazine dan Atropine Sulfate terhadap Profil Hematologi Anjing Lokal Tri Utami; Tarsisius Considus Tophianong; Yohanes T.R.M.R Simarmata; Yeremia Yobelanno Sitompul; Yustinus O.P Wuhan; Jayusman Arsiyanti
JURNAL KAJIAN VETERINER Vol 14 No 1 (2026): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v14i1.25245

Abstract

This study aimed to evaluate the effect of the combination of ketamine-medetomidine anesthesia with two different types of premedication, namely acepromazine and atropine sulfate, on hematological parameters in local dogs. A total of six male local dogs aged 1–2 years with a body weight of 8–15 kg were used as experimental animals and randomly divided into two groups (three dogs each). Group 1 was given acepromazine premedication (0.05 mg/kg BW, im), while group 2 was given atropine sulfate premedication (0.04 mg/kg BW, sc). Fifteen minutes later, both groups were induced with ketamine (10 mg/kg BW, im) and medetomidine (30 µg/kg BW, im). Blood samples were taken through the saphenous vein at three observation times: before anesthesia (T0), 30 minutes (T30), and 60 minutes (T60) after anesthesia. Parameters measured included total erythrocytes, hemoglobin levels, hematocrit values, and total leukocytes using a hematology analyzer. Data analysis showed no significant differences (p>0.05) between time points or between treatment groups. The erythrocyte, hemoglobin, hematocrit, and leukocyte counts in both groups remained within the normal physiological range. However, the group 1 showed a tendency for decreased hematological values after anesthesia due to the sedative effect of acepromazine, while the group 2 showed a transient increase due to the pre-anesthetic stress response and mild dehydration. The results of this study indicate that the combination of ketamine-medetomidine anesthesia with acepromazine or atropine sulfate premedication does not cause significant hematological changes and is safe for use in local dogs during clinical anesthetic procedures.
Co-Authors A.A. Ketut Agung Cahyawan W Afif Muhammad Akrom Akrom, Afif Muhammad Alfarisa Nururrozi Alfarisa Nururrozi, Alfarisa Almet, Julianty Amalo, Maria Dangur, Fransiska Aping Deta, Stepanus Umbu Dewi F.L Djungu Diana Agustiani Wuri Diana Agustiani Wuri Foeh, Nancy Diana Frederika Katerina Jayusman Arsiyanti Joesoef, Jayusman Arsiyanti Juni Claudia Dami Kallau, Novalino Harold Geoffrey Larry R. W. Toha Loe, Fhady Risckhy Malelak, Marlin Cindy Claudya Maria Aega Gelolodo Maxs Urias Ebenheizer Sanam Nancy D. F. K. Foeh Nancy D. F. K. Foeh Permana, Rief Ghulam Satria Rief Ghulam Satria Permana Sidna Artanto Sidna Artanto, Sidna Simarmata, Yohanes Timbun Raja Mangihut Ronael Simarmata, Yohanes Timbun Raja Mangiut Ronael Slamet Raharjo Slamet Raharjo Soedarmanto Indarjulianto Tabali, Zulkifli Tarsisius Considus Tophianong Tarsisius Considus Tophianong Tarsisius Considus Tophianong, Tarsisius Considus Toha, Larry Richard Wellem Tri Utami Tri Utami Tri Utami Trini Susmiati Yanuartono Yanuartono Yanuartono, Yanuartono Yohanes T.R.M.R Simarmata Yustinus O.P Wuhan Yustinus Oswin Primajuni Wuhan