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Journal : Journal of Saintech Transfer

Microbial Content Test on Sliced Papaya in Area of Universitas Sumatera Utara Lubis, Nenni Dwi Aprianti; Amelia, Sri; Balatif, Ridwan
Journal of Saintech Transfer Vol. 3 No. 2 (2020): Journal of Saintech Transfer
Publisher : Talenta Publisher Universitas Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jst.v3i2.3967

Abstract

Papaya is a fruit that is often consumed in Indonesia and has increased consumption from 2015-2016 based on SUSENAS 2016. Papaya has high water content (85-90%) and the pH is near neutral. This makes papaya vulnerable and suitable medium for the growth of pathogenic microbes. The presence of pathogenic microbes in papaya can cause health problems for those who consumed them. Objectives this study was to observe the microbial content of sliced papaya in the Universitas Sumatera Utara. Method this research was carried out by conducting laboratory tests and the results obtained in the form of a description of microbial content in papaya cut samples. The content of coliform microbes in sliced papaya was tested using the Most Probable Number (MPN) method and biochemical identification. Papaya samples were selected using total sampling method from the University of Sumatera Utara. Results A total of 14 sliced papaya samples were analysed in this study. By using the MPN test it was found that all papaya samples had a microbial threshold exceeding those determined by SNI 7388: 2009 which is below <20 / g. A total of 9 papaya samples (64.28%) contained Klebsiella sp. bacteria, 5 samples (35.72%) contained E.coli bacteria. Conclusions Bacteria found in this study were Escherichia coli and Klebsiella sp. The presence of microbial content in the sliced papaya sample that exceeds the threshold must be a concern of various parties in the surveillance of snacks being sold.
The Number of Bacteria Colonies in Carp Fish (Cyprinus carpio) After Administration of Lime (Citrus aurantifolia) and Orange Extract (Citrus jambhiri Lush.) Lubis, Nenni Dwi Aprianti
Journal of Saintech Transfer Vol. 5 No. 1 (2022): Journal of Saintech Transfer
Publisher : Talenta Publisher Universitas Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jst.v5i1.8694

Abstract

Fish is one of the most commonly consumed protein sources. Fish are at risk as a medium for the growth of microbial contaminants that can cause foodborne diseases. This disease can cause diarrhea and even death.Contaminant microbes can be found in foods consumed raw, such as Naniura, a typical Batak cuisine in the form of raw carp. Various natural spices such as lime and orange also have potential as antimicrobials. This study aimed to determine the antimicrobial potential of lime and orange juice in fresh carp samples. This study used extracts of lime and orange with concentrations of 6.25%, 12.5%, 25%, and 50%, respectively. The sample used is carp meat. The bacteria present in the sample were counted using the Total Plate Count (TPC) method before and after lime and lime extract administration. The results showed that before administering the two extracts, the total colonies in the sample could not be counted. The antimicrobial effect of orange juice extract began to be seen at a concentration of 12.5%, while in lime, it was only seen at a concentration of 50%. From the results of this study, it was concluded that orange juice extract had a better antimicrobial effect than lime.