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All Journal International Journal of Public Health Science (IJPHS) Jurnal Teknologi Laboratorium Jurnal Penelitian Kesehatan Suara Forikes THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Analis Medika Biosains (JAMBS) INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY JPP Jurnal Kesehatan Poltekkes Palembang Jaringan Laboratorium Medis Medica Arteriana (Med-Art) Medicra (Journal of Medical Laboratory Science/Technology) CENDEKIA : Jurnal Ilmu Pengetahuan Devotion: Journal of Research and Community Service International Journal of Advanced Health Science and Technology IBTIDA : Jurnal Kajian Pendidikan Dasar HEALTHY: Jurnal Inovasi Riset Ilmu Kesehatan Jurnal Sehat Indonesia (JUSINDO) Sewagati Analis Kesehatan Sains (e-Journal) Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat Jurnal Pengabdian Kesehatan Beguai Jejama Frontiers in Community Service and Empowerment Health Dynamics Jurnal Ilmu Kesehatan Folia Medica Indonesiana
Suhariyadi
Department Of Medical Laboratory Technology, Poltekkes Kemenkes Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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PERBANDINGAN STATUS ANTI-HBs (Hepatitis B surface Antibody) DAN JUMLAH LIMFOSIT PADA PESERTA PROGRAM VAKSINASI HEPATITIS B DENGAN DAN TANPA KOMORBID DIABETES MELITUS TIPE 2 Widiastutik, Dyah; Suhariyadi, Suhariyadi; Rahayuningsih, Christ. Kartika; Woelansari, Evy Diah
HEALTHY : Jurnal Inovasi Riset Ilmu Kesehatan Vol. 4 No. 4 (2025)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/healthy.v4i4.6582

Abstract

Anti-HBs and lymphocyte count are used to assess the success of hepatitis B vaccination. Healthcare workers are at high risk of contracting hepatitis B, making routine vaccination essential. This study was conducted at RSUD Caruban, Madiun Regency, involving 44 participants of the hepatitis B vaccination program, both with and without type 2 diabetes mellitus comorbidity. Examinations were carried out six months post-vaccination. The study employed a comparative analytical method with a cross-sectional approach and was conducted in February 2025. The instruments used were the Eclia-Mindray CL-900i with the ELISA method and the Sysmex XN-350 with the Flow Cytometry method. In the group with type 2 diabetes mellitus, the average Anti-HBs titer was 77.53 mIU/mL, whereas in the non-diabetic group it was 882.06 mIU/mL. The average lymphocyte count was also higher in the non-diabetic group (38.91%) compared to the group with diabetes mellitus (22.52%), although both were within the normal range. There were differences in Anti-HBs status and lymphocyte counts between hepatitis B vaccination participants with and without type 2 diabetes mellitus comorbidity. ABSTRAKAnti-HBs dan jumlah limfosit digunakan untuk menilai keberhasilan vaksinasi hepatitis B. Tenaga kesehatan memiliki risiko tinggi tertular hepatitis B, sehingga vaksinasi rutin diperlukan. Penelitian ini dilakukan di RSUD Caruban Kabupaten Madiun, pada 44 peserta vaksinasi hepatitis B, baik dengan maupun tanpa komorbid diabetes melitus tipe 2. Pemeriksaan dilakukan enam bulan setelah vaksinasi. Penelitian ini menggunakan metode komparatif analitik dengan pendekatan cross-sectional, dilaksanakan pada bulan Februari tahun 2025. Instrumen yang digunakan adalah Eclia-Mindray CL-900i, dengan metode ELISA dan Sysmex XN-350, dengan metode Flow Cytometry. Pada kelompok dengan diabetes melitus tipe 2, rata-rata titer Anti-HBs adalah  77,53 mIU/mL, sementara kelompok tanpa diabetes melitus rata-ratanya adalah 882,06 mIU/mL. Untuk jumlah limfosit rata-ratanya lebih tinggi pada kelompok tanpa diabetes melitus yaitu sebesar 38,91 % dibandingkan kelompok dengan komorbid diabetes melitus tipe 2 sebesar 22,52%, meskipun keduanya dalam batas normal. Terdapat perbedaan status Anti-HBs dan jumlah limfosit pada peserta program vaksinasi hepatitis B dengan dan tanpa komorbid diabetes melitus tipe 2.
HUBUNGAN JUMLAH SEL LEUKOSIT MONONUKLEAR (MONOSIT, LIMFOSIT) DENGAN HASIL UJI TUBEX TF TERHADAP PASIEN SUSPEK DEMAM TIFOID Kumahartati, Intan; Woelansari, Evy Diah; Istanto, Wisnu; Suhariyadi, Suhariyadi
CENDEKIA: Jurnal Ilmu Pengetahuan Vol. 5 No. 4 (2025)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/cendekia.v5i4.7143

Abstract

Typhoid fever can be diagnosed in suspected patients through the Tubex TF test, which has a relatively high sensitivity and specificity of 73%. Typhoid fever can also be diagnosed by examining the number of mononuclear leukocytes, particularly monocytes and lymphocytes, which are indicators of bacterial infection. Typhoid fever is a disease caused by Salmonella typhi. Typhoid fever cases in Indonesia range from 350 to 810 per 100,000 people, with a prevalence of 1.6%. The purpose of this study was to determine the relationship between the number of mononuclear leukocytes (monocytes and lymphocytes) and Tubex TF results in patients suspected of typhoid fever. This study was an analytical observational study and the sampling technique used was consecutive sampling, with all subjects meeting the selection criteria, amounting to 63 samples. The number of mononuclear leukocytes (monocytes, lymphocytes) was determined using a Hematology Analyzer and the Tubex TF test was determined using the IMBI method. Spearman's correlation test was used for statistical analysis. The findings showed a significant correlation between the number of monocytes and the Tubex TF results in patients suspected of typhoid fever (p = 0.006 < ? = 0.05). In contrast, no significant correlation was found between the number of lymphocytes and the Tubex TF results in the same group of patients (p = 0.296 > ? = 0.05). ABSTRAKDiagnosis demam tifoid pada pasien suspek dapat dilakukan melalui uji Tubex TF yang menunjukkan sensitivitas dan spesifisitas yang relative tinggi, yaitu sebesar 73%. Demam tifoid dapat pula didiagnosis dengan pemeriksaan jumlah sel leukosit mononuklear, khususnya sel monosit dan sel limfosit yang akan menjadi indikator ketika terjadi infeksi akibat bakteri. Demam tifoid adalah penyakit yang disebabkan oleh infeksi bakteri Salmonella typhi. Kasus demam tifoid di Indonesia berkisar antara 350 hingga 810 per 100.000 penduduk, dengan prevalensi 1,6%. Tujuan penelitian ini, untuk mengetahui hubungan jumlah sel leukosit mononuklear (monosit, limfosit) dengan hasil Tubex TF pada pasien suspek demam tifoid. Penelitian ini merupakan penelitian observasional analitik dan juga teknik pengambilan sampelnya ialah dengan cara Consecutive Sampling yaitu semua subyek yang memenuhi kriteria pemilihan yaitu sebanyak 63 sampel. Jumlah sel leukosti mononuclear (monosit, limfosit) ditentukan dengan alat Hematology Analyzer dan uji Tubex TF ditentukan dengan metode IMBI. Uji korelasi Spearman digunakan untuk analisis statistik. Temuan menunjukkan adanya hubungan yang signifikan antara jumlah monosit dan hasil Tubex TF pada pasien yang diduga demam tifoid (p = 0,006 < ? = 0,05). Sebaliknya, tidak ditemukan korelasi yang signifikan antara jumlah limfosit dan hasil Tubex TF pada kelompok pasien yang sama (p = 0,296 > ? = 0,05).
Efforts to Control Blood Pressure for Prolanis Hypertension Members through the Utilization of Bay Leaf Decoction Handayati, Anik; Suhariyadi; Pestariati
Frontiers in Community Service and Empowerment Vol. 4 No. 3 (2025): September
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ficse.v4i3.104

Abstract

Bay leaves are a type of kitchen spice that is often added to dishes, inexpensive, and easily obtained. Research results have proven that the active compounds in bay leaves are quite effective and safer in lowering blood pressure. Most of the Sreseh residents work as fishermen who tend to consume salty foods, which causes a high incidence of hypertension. Therefore, it is necessary to increase knowledge about healthy lifestyles and the use of herbal medicine made from boiled bay leaves to lower blood pressure among Prolanis hypertension members in the Sreseh Health Center area. The methods used in this community service activity are lecture/presentation and demonstration methods. Lecture activities are carried out by delivering material on the prevention and natural treatment of hypertension using bay leaves, the benefits of bay leaves, and the processing of bay leaves for hypertension treatment. The demonstration method is carried out by showing how to process bay leaves. The target of the activity is Prolanis hypertension patients in Noreh Village, within the working area of the Sreseh Health Center, Sampang, Madura. Members of the Chronic Disease Management Program (PROLANIS), especially those with hypertension, were very enthusiastic and motivated to participate in every stage of the community service activity, from blood pressure checks, Prolanis exercises, to education on the benefits of bay leaves. After consuming boiled bay leaves, blood pressure checks on Prolanis members with hypertension showed a 30% decrease in the number of Prolanis members with hypertension, from the previous 76.67% to 46.67%.
Comparison of Platelet Index in Type 2 Diabetes Mellitus Patients With and Without Diabetic Ulcers Rahma Yuliana, Putri; Suhariyadi, Suhariyadi; Woelansari, Evy Diah
Devotion : Journal of Research and Community Service Vol. 6 No. 11 (2025): Devotion: Journal of Community Research
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/devotion.v6i11.25573

Abstract

Hyperglycaemia is caused by poor insulin production and insulin resistance, which is the beginning of type 2 diabetes mellitus. The blockage of blood vessels caused by this disease is due to the increase in blood sugar levels, which is the cause of diabetic ulcers. As a result, patients are often unaware of their injuries. This research aims to identify differences in platelet indices (MPV, PDW and PCT) in people with type 2 diabetes mellitus who have diabetic ulcers and those who do not. This research uses observational analysis methods and a cross-sectional analysis approach. The independent variable in this study was type 2 diabetes mellitus patients with and without diabetic ulcers, while the dependent variable in this study was the platelet value, consisting of MPV in type 2 diabetes mellitus patients with and without diabetic ulcers of 7,716 fL and 7,837 fL. The mean PCT in type 2 diabetes mellitus patients with and without diabetic ulcers was 0.225% and 0.245%, respectively. The mean PDW in type 2 diabetes mellitus patients with and without diabetic ulcers was 18.825% and 14.237%, respectively. The results of data analysis using unpaired t-test showed a p-value of 0.00 for PDW and 0.002 for MPV, so it was concluded that there were differences in MPV and PDW in type 2 diabetes mellitus patients who had diabetic ulcers and those who did not.
Lymphocyte Count and SARS-CoV-2 Antibody Level in Healthy Donors' Blood at an Indonesian Blood Transfusion Center Prasasti, Adelia Gita; Woelansari, Evy Diah; , Suhariyadi; Anggraini, Anita Dwi
Folia Medica Indonesiana Vol. 59, No. 4
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Highlights: 1. It is essential to conduct research on SARS-CoV-2 for the purpose of acquiring further understanding, especially concerning the production of antibodies examined using antibody titer blood tests. 2. Although the relationship between the examined variables is not significant, this study offers valuable information on blood test results after the COVID-19 vaccination, which can serve as scientific evidence for further research. Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a virus that infects the respiratory system by attacking the mucous and epithelial cells. This infection commonly leads to an increase in lymphocyte count as an immune response to invading pathogens. Moreover, antibodies bind and inactivate foreign substances to destroy pathogens and inhibit their replication. These mechanisms prompt the objective of this study, which was to define the relationship between lymphocyte count and SARS-CoV-2 antibody level. This analytical observational study used a cross-sectional approach with quantitative analysis methods and purposive sampling. Healthy donors who had received coronavirus disease (COVID-19) vaccines provided the samples for this study. A total of 30 blood samples were collected from the Blood Transfusion Center of the Indonesian Red Cross Surabaya Area. This study was conducted in May 2022 at two distinct locations. The examination of lymphocytes was carried out using the flow cytometry method in the Hematology Laboratory, Department of Medical Laboratory Technology, Politeknik Kesehatan Kemenkes Surabaya, Surabaya, Indonesia. In addition, the antibody titer test using the enzyme-linked immunosorbent assay (ELISA) method was performed in the Immunoserology Laboratory of the Surabaya Health Laboratory Center, Surabaya, Indonesia. The analysis revealed an average lymphocyte concentration of 2.2633 x 103/µl and an average antibody level of 0.2197 according to the optical density (OD) ratio. The data analysis was performed using Spearman's rank correlation statistical test (p<0.005), and the results indicated a lack of significance with p=0.262. In conclusion, there is no relationship between total lymphocyte count and SARS-CoV-2 antibody level.
Development of a Rapid Diagnostic Method for Simultaneous Detection of Streptococcus viridans in Cases of Heart Disease Pestariati Pestariati; Suhariyadi Suhariyadi; Asryadin Asryadin; Nilasari Indah Yuniati
Jurnal Penelitian Pendidikan IPA Vol 11 No 11 (2025): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i11.12730

Abstract

Heart disease is a leading cause of death worldwide, with a complex pathophysiology often involving interactions between genetic factors, the environment, and pathogenic microorganisms. Streptococcus viridans is a clinically significant pathogenic bacterium associated with infections of the cardiovascular system, including infective endocarditis, pericarditis, and other complications. However, timely and accurate diagnosis of this bacterial infection in cases of acute heart disease is often challenging, requiring rapid and sensitive diagnostic methods. Currently, diagnostic methods for detecting Streptococcus viridans in cases of acute heart disease tend to be time-consuming; therefore, developing a rapid diagnostic method that can detect both bacteria simultaneously is crucial. The aim of this study is to develop a rapid and sensitive diagnostic method for detecting the presence of Streptococcus viridans in samples from patients with heart disease. The method used is the identification of specific genes, the design of primer sequences, and the design of probes using specific 16s rRNA genes using bioinformatics techniques. Based on the research results obtained primer pair sequences are: oligonucleotide primer forward 5’-GCGACGATACATAGCCGAC-3’; primer reverse is 3’- CGAGCCAGTCTGAAAGC-5’, while the probe sequence is 5’-GACTGAGACACGGCCCAGACTC-3’. Primer and probe pair quality tests showed very good primer and probe quality for amplification with a 120 bp amplification product. Suggestions in the research are that it is necessary to continue with qPCR optimization to determine the melting temperature which is then carried out sensitivity tests of primer pair sequences and specific 16s rRNA Streptococcus viridans gene probes.
The Comparison of Variant Index Score (VIS) of Homemade and Commercial Lyophilized Serum Ilma Ainun Nisa; Amalia Putri Khurota Ayyun; Anik Handayati; Museyaroh; Suhariyadi
International Journal of Advanced Health Science and Technology Vol. 3 No. 6 (2023): December
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v3i6.283

Abstract

Many laboratory installations have not implemented quality assurance periodically due to the high cost of purchasing control materials. This study aims to determine the difference in Variant Index Score value of homemade lyophilized serum with commercial parameters of SGOT, SGPT, creatinine, BUN, glucose, uric acid, triglycerides, and cholesterol. The research was conducted in January–May 2023 at the Surabaya Health Center laboratory, the reference laboratory, and the Ubaya Technobiology Faculty using descriptive comparative research with a quantitative approach. The test material used was homemade lyophilized serum, with the research variable being the VIS value of lyophilized serum. The results of the analysis showed a comparison of the VIS value of homemade and commercial serum based on the participant's target values of SGOT, SGPT, creatinine, BUN, glucose, triglycerides, and cholesterol parameters obtained a significance value of 00,759; 0,983; 0,160; 0,994; 0,413; 0,797; 0,318; and 0,262, and based on the true value target values of SGOT, SGPT, creatinine, BUN, glucose, uric acid, triglycerides, and cholesterol parameters obtained a significance value of 0,70; 0,973; It is concluded that there is no significant difference in VIS value between homemade and commercial lyophilized serum, so homemade lyophilized serum can be used as an option for control material to replace commercial serum for laboratory quality assurance.
Optimizing the Role of Posyandu and Posbindo Cadres in Alleviating Stunting and Preventing Non-Communicable Diseases in Pacar Kembang Village, Surabaya Suhariyadi Suhariyadi; Anik Handayati; Pestariati Pestariati
Frontiers in Community Service and Empowerment Vol. 5 No. 2 (2026): June
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ficse.v5i2.126

Abstract

Stunting, a significant health problem in Indonesia, must be addressed. Stunting is defined as a condition caused by inadequate nutritional intake that does not meet nutritional needs. Integrated Service Posts (Posyandu) are a way to provide health services to the community and have the ability to develop resources, especially in health matters. The methods used in this community service activity are lecture/presentation and discussion and question and answer methods. The lecture activities included presenting material on the importance of balanced nutrition during the first 1000 days of life (HPK), explaining the role of cadres in providing simple counseling to the community, facilitating discussions and question-and-answer sessions, and concluding the session. Overall, the implemented activities showcase the active participation of both the cadres and the community. These results serve as the basis for implementing further activities in the form of monitoring, evaluation, and more targeted health interventions.
Mentoring and Improving Knowledge on the Benefits of Garlic in Reducing Blood Cholesterol Levels among Hypertensive Prolanis Members Anik Handayati; Suhariyadi Suhariyadi; Pestariati Pestariati
Frontiers in Community Service and Empowerment Vol. 5 No. 2 (2026): June
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ficse.v5i2.130

Abstract

Hypertension is a non-communicable disease that is a major cause of stroke and coronary heart disease. It is commonly found in the community and is a significant contributor to mortality and disability worldwide. A preliminary study showed that more than 50% of Prolanis members in Bangsah Village, Sreseh Subdistrict, Sampang Regency had high cholesterol levels; therefore, alternative supplements are needed to reduce blood cholesterol levels. Garlic is a food ingredient that is widely available in the community but has not been widely utilized as a cholesterol-lowering supplement. Garlic contains allicin, which has strong antimicrobial, anti-inflammatory, and antioxidant properties. It also contains ajoene, saponins, flavonoids, and various sulfur compounds that contribute to its health benefits. Studies have shown that garlic consumption can help reduce total cholesterol and LDL levels. Therefore, education on processing garlic into black garlic is highly needed to reduce cholesterol levels among hypertensive Prolanis members. The results of the community service activity showed that the program was implemented smoothly and successfully, starting from the survey and coordination stage, the initial assessment, program implementation, and evaluation. The success of this PKM activity is expected to be beneficial for the wider community, especially regarding the use of garlic to reduce cholesterol levels. The evaluation results showed a 17% reduction in the number of hypertensive Prolanis members with high cholesterol levels.
ASSOCIATION BETWEEN TRIGLYCERIDE SERUM LEVELS AND GLOMERULAR FILTRATION RATE (eGFR) IN PATIENTS WITH CHRONIC RENAL FAILURE AT JEMURSARI ISLAMIC HOSPITAL SURABAYA, INDONESIA Uswatun Hasanah; Suhariyadi; Andreas Putro Ragil Santoso
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 2 (2020): Increasing Research in Diagnostic Laboratory Testing
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v2i2.1668

Abstract

Chronic Renal Failure (CRF) is a progressive and irreversible decrease in kidney function. One risk factor that affects the progression of CRF is dyslipidemia due to abnormalities of lipid metabolisms. Dyslipidemia is characterized by the increase level of total cholesterol, LDL cholesterol, triglycerides, and a decrease in HDL cholesterol level. eGFR is a parameter for assessing the excretion function, by calculating the amount of filtrate produced by the renal glomerulus. This study aims to determine the relationship between serum triglyceride levels with eGFR in patient suffering from CRF at Jemursari Islamic Hospital Surabaya. This type of research is descriptive experimental with cross–sectional design. The study population was the patients with CRF. The primary data in this study was the results of examination of serum triglyceride levels in patients with CRF. The secondary data (serum creatinine levels, body weight, age, and sex) was obtained from medical records of CRF patients in February 2019. As much as 12% of patients were in the 40–50 years old category, 36% of patients were 51–60 years old, 44% of patients were 61–70 years old, and 8% of patients were 71–80 years old. 72% of patients were male. The mean triglyceride level was 146.68 mg/dL and the average eGFR was 19.86 mL/min/1.73m2. The relationships between triglyceride levels and eGFR was carried out by the Spearman–rho test where r value was –0.442 and value was 0.027. It was concluded that there was a significant negative correlation between serum triglyceride levels and eGFR in patients with CRF.
Co-Authors ., Pestariati Abror, Yogi Khoirul Alifiah, Salsabiil Rochman Aliya Ayu Rizqiyah Amalia Putri Khurota Ayyun Andreas Putro Ragil Santoso, Andreas Putro Ragil Anggraini, Anita Dwi Ariyani, Ahna Fitri Asryadin Asryadin Cheppy Juwita Ayuningrum Diah Titik Mutiarawati Edy Haryanto Eka Safitri, Devayanti Eko, Sugeng Endah Astuti, Sri Sulami Endarini, Lully Hanni Erin Febrianti Evy Diah Woelansari Fortuna Aliza Dewi Handayati, Anik Ilma Ainun Nisa Istanto, Wisnu istiqomah istiqomah Istiqomah Istiqomah Juliana Christyaningsih Kanayu Aulia Masardi Kartika Rahayuningsih, Christ Krihariyani, Dwi Kumahartati, Intan Liliek Soetjiatie Luthfi Rusyadi Mamik Mamik Mamik Mamik Melly Nury Sabriyani MINARTI Minarti Minarti Museyaroh, Museyaroh Mutiarawati, Diah Titik Nilasari Indah Yuniati Niswata, Navila Arfianti Novikasari, Caturizkyana Nur Alizah Octarina, Nofita Prasasti, Adelia Gita Purwati Purwati Rahma Yuliana, Putri Rizqiyah, Aliya Ayu Salsabiil Rochman Alifiah Sasongkowati, Retno Shela Yuni Arta Siti Helmyati Sofiyah Sofiyah Sri Utami Suliati Suliati Syamsul Arifin Uswatun Hasanah Vernanda Amyarti Lailatul Syafitri Widiastutik, Dyah Yuninda Zebina Salsabillah