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All Journal International Journal of Public Health Science (IJPHS) Jurnal Teknologi Laboratorium Jurnal Penelitian Kesehatan Suara Forikes THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Analis Medika Biosains (JAMBS) INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY JPP Jurnal Kesehatan Poltekkes Palembang Jaringan Laboratorium Medis Medica Arteriana (Med-Art) Medicra (Journal of Medical Laboratory Science/Technology) CENDEKIA : Jurnal Ilmu Pengetahuan Devotion: Journal of Research and Community Service International Journal of Advanced Health Science and Technology IBTIDA : Jurnal Kajian Pendidikan Dasar HEALTHY: Jurnal Inovasi Riset Ilmu Kesehatan Jurnal Sehat Indonesia (JUSINDO) Sewagati Analis Kesehatan Sains (e-Journal) Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat Jurnal Pengabdian Kesehatan Beguai Jejama Frontiers in Community Service and Empowerment Health Dynamics Jurnal Ilmu Kesehatan Folia Medica Indonesiana
Suhariyadi
Department Of Medical Laboratory Technology, Poltekkes Kemenkes Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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The Relationship Between TSH and Indirect Bilirubin Levels in Neonates Suspected of Having Jaundice Nur Alizah; Anik Handayati; Museyaroh Museyaroh; Suhariyadi Suhariyadi
Health Dynamics Vol 1, No 10 (2024): October 2024
Publisher : Knowledge Dynamics

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hd11006

Abstract

Background: Congenital hypothyroidism (CH) is a condition of thyroid hormone deficiency that occurs at birth. The TSH (thyroid-stimulating hormone) test is crucial for diagnosing hypothyroidism. CH is known to cause prolonged unconjugated hyperbilirubinemia. Therefore, this study aimed to analyze the relationship between TSH and indirect bilirubin levels in neonates suspected of having jaundice. Methods: This is a non-experimental, retrospective study conducted at Lombok Dua Dua Lontar Mother and Child Hospital in Surabaya. The study involved data collection on neonates aged 2–7 days suspected of jaundice, whose TSH and indirect bilirubin levels were measured between November 2022 to April 2024. Results: Among 100 neonates, 62% were aged 2-4 days, while 38% were aged 5-7 days. The majority were male (56%), with female comprising 44%. Of the 100 neonates, only 1 (1%) had borderline TSH levels, while 99% had normal TSH levels. Hyperbilirubinemia was observed in 94% of the neonates, while 6% had normal indirect bilirubin levels. Statistical analysis using the Spearman correlation showed no significant link between TSH and indirect bilirubin levels (p = 0.802). Conclusions: While this study did not find a clear connection between TSH and indirect bilirubin levels in neonates suspected of having jaundice, one case of borderline TSH was identified. This neonate required referral to pediatric endocrinology, as untreated congenital hypothyroidism can lead to mental retardation. Despite limited research linking TSH and bilirubin levels in jaundiced neonates, routine screening for congenital hypothyroidism using TSH testing should be reconsidered. Future studies could benefit from focusing on specific causes of neonatal jaundice to help narrow down research questions in this area.
Perbandingan Pemeriksaan Skrining Antibodi (Indirect Coombs Test) dengan Direct Coombs Test pada Pasien dengan Riwayat Transfusi Darah Berulang Shela Yuni Arta; Suhariyadi Suhariyadi; Evy Diah Woelansari; Christ Kartika Rahayuningsih
Jaringan Laboratorium Medis Vol 7 No 2 (2025): November 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i2.13643

Abstract

Repeated blood transfusions can increase risk of alloimmunization and autoimunization, which causes formation alloantibodies and autoantibodies. The presence of these antibodies can make cross-matching more difficult and increase the possibility of hemolytic transfusion risk. Antibody screening tests (Indirect Coombs Test) and Direct Coombs Test play a role in detecting the presence of alloantibodies and autoantibodies in patient with a history of repeated transfusion. The aim of the study was to determine the diferences in results between antibody screening (Indirect Coombs Test) and Direct Coombs Test in patients receiving repeated blood transfusions. This study used a quasi-experimental design with a cross-sectional approach. The subjects were patients with a history of blood transfusions more than 5 times at RSUD R.T. Notopuro Sidoarjo totalling 60 samples. The data obtained were then analyzed statistically to see if there were any significant differences between the two types of examinations. The results obtained showed a significant difference between the results of the antibody screening test (ICT) and the Direct Coombs Test (DCT) (p-value 0.000), that more patients with repeated blood transfusions had positive DCT results (75%). Some patients showed positive results on one or both types of tests, indicating the presence of alloantibodies or autoantibodies. ICT and DCT tests provide complementary information in detecting antibodies in patients with repeated transfusions. The use of both is important in improving the safety of blood transfusions and minimizing the risk of hemolytic reactions.
Gambaran Pemeriksaan Screening Hepatitis B dan Hepatitis C pada Ibu Hamil dengan Metode Imunokromatografi di Puskesmas Sidoarjo Melly Nury Sabriyani; Evy Diah Woelansari; Suhariyadi; Wisnu Istanto
JIKES : Jurnal Ilmu Kesehatan Vol. 5 No. 1 (2026): Juli-Desember 2026
Publisher : Yayasan Pendidikan Tanggui Baimbaian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71456/jik.v5i1.2552

Abstract

Hepatitis B dan Hepatitis C adalah penyakit infeksi yang dapat ditularkan dari ibu kepada bayi, terutama selama proses kehamilan dan persalinan. Pemeriksaan skrining pada ibu hamil penting dilakukan sebagai upaya deteksi dini dan pencegahan penularan. Penelitian ini bertujuan untuk memperoleh gambaran hasil pemeriksaan skrining Hepatitis B dan Hepatitis C pada ibu hamil menggunakan metode imunokromatografi di Puskesmas Sidoarjo. Penelitian ini menggunakan desain deskriptif dengan pendekatan cross-sectional. Sampel penelitian terdiri atas 30 ibu hamil trimester I, II, dan III yang menjalani pemeriksaan antenatal care (ANC) di Puskesmas Sidoarjo. Pemeriksaan dilakukan menggunakan rapid test HBsAg dan Anti-HCV dengan metode imunokromatografi. Hasil penelitian menunjukkan bahwa seluruh responden memperoleh hasil nonreaktif pada pemeriksaan Hepatitis B menggunakan rapid test HbsAg sebanyak 30 orang (100%) dan pemeriksaan Hepatitis C menggunakan rapid test Anti-HCV sebanyak 30 orang (100%). penelitian ini menunujukkan tidak ditemukan infeksi Hepatitis pada ibu hamil di Puskesmas Sidoarjo. Diharapkan peneliti lanjutan menggunakan metode pemeriksaan yang lebih akurat dan sensitif, seperti ELISA atau PCR.
PERBANDINGAN KADAR HIGH SENSITIVITY C-REACTIVE PROTEIN (hs-CRP) METODE Fluoroescence Immunoassay (FIA) ANTARA SAMPEL SERUM DAN PLASMA EDTA PADA PENDERITA DIABETES MELITUS Kanayu Aulia Masardi; Suhariyadi Suhariyadi; Wisnu Istanto; Evy Diah Woelansari
HEALTHY : Jurnal Inovasi Riset Ilmu Kesehatan Vol. 5 No. 4 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/healthy.v5i4.13729

Abstract

Diabetes mellitus is a chronic metabolic disorder associated with increased inflammatory activity and contributes to the development of cardiovascular complications. High-sensitivity C-reactive protein (hs-CRP) serves as an inflammatory marker to evaluate low-grade systemic inflammation. Accurate specimen selection is crucial for analytical validity. This study aims to compare hs-CRP levels measured by Fluorescence Immunoassay (FIA) between serum and EDTA plasma specimens in patients with diabetes mellitus. An observational comparative study with a cross-sectional design was conducted using 20 paired serum and EDTA plasma samples from diabetes mellitus patients at Haji General Hospital, East Java Province, in March 2026. hs-CRP levels were measured using the FIA method. The mean hs-CRP concentration in serum was  ( ) and in EDTA plasma was  ( ). The Wilcoxon Signed Rank Test yielded a  of 0.463 ( ), indicating no statistically significant difference between serum and EDTA plasma hs-CRP levels. In conclusion, serum and EDTA plasma demonstrate analytical equivalence for hs-CRP measurement using the FIA method, allowing EDTA plasma to serve as a valid alternative specimen in routine laboratory practice. ABSTRAK Diabetes melitus merupakan gangguan metabolisme kronis yang berkaitan dengan peningkatan aktivitas inflamasi dan berkontribusi terhadap perkembangan komplikasi kardiovaskular. High-sensitivity C-reactive protein (hs-CRP) digunakan sebagai penanda inflamasi untuk menganalisis proses peradangan kronis tingkat rendah (low-grade inflammation). Ketepatan pemilihan spesimen laboratorium sangat memengaruhi validitas analitik. Penelitian ini bertujuan untuk membandingkan kadar hs-CRP metode Fluorescence Immunoassay (FIA) antara spesimen serum dan plasma EDTA pada penderita diabetes melitus. Penelitian observasional komparatif dengan desain cross-sectional ini dilakukan terhadap 20 pasang spesimen serum dan plasma EDTA dari pasien diabetes melitus di RSUD Haji Provinsi Jawa Timur pada Maret 2026. Kadar hs-CRP diukur menggunakan metode FIA. Hasil penelitian menunjukkan rerata kadar hs-CRP serum sebesar  ( ) dan plasma EDTA sebesar  ( ). Hasil uji Wilcoxon Signed Rank Test menghasilkan nilai  ( ), yang menunjukkan tidak terdapat perbedaan yang bermakna secara statistik antara kadar hs-CRP pada spesimen serum dan plasma EDTA. Kesimpulannya, serum dan plasma EDTA memiliki kesepadanan analitik dalam pemeriksaan hs-CRP metode FIA, sehingga plasma EDTA dapat digunakan sebagai spesimen alternatif yang valid dalam kondisi darurat laboratorium.  
HBsAg Screening Using Immunochromatography Method in Preoperative Patients at Dr. Soegiri Hospital, Lamongan Yuninda Zebina Salsabillah; Suhariyadi; Museyaroh; Anik Handayati
Jurnal Sehat Indonesia (JUSINDO) Vol. 8 No. 2 (2026): Jurnal Sehat Indonesia (JUSINDO)
Publisher : CV. Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/-.v8i2.559

Abstract

Patients undergoing surgery require HBsAg screening to determine their hepatitis B infection status. Hepatitis B remains a global health concern with a relatively high incidence rate, including in Indonesia. This study aimed to describe the results of HBsAg screening using the immunochromatographic method among preoperative patients at Dr. Soegiri Lamongan Hospital. This study employed a descriptive design with a cross-sectional approach. The research sample consisted of 100 preoperative patients selected using purposive sampling. HBsAg detection was performed using an immunochromatographic rapid test method. Data were analyzed using frequency distribution. The results showed that most preoperative patients had negative HBsAg results, with 92 samples (92%), while positive results were identified in 8 samples (8%), consisting of 4 male and 4 female patients aged 46–60 years. Based on these findings, it was concluded that the majority of preoperative patients tested negative for HBsAg, while positive HBsAg results were detected in a small proportion of samples.
GAMBARAN HASIL PEMERIKSAAN ANTIBODI HEPATITIS B DAN HEPATITIS C PADA IBU HAMIL DENGAN METODE IMUNOKROMATOGRAFI DI PUSKESMAS SIDOARJO Vernanda Amyarti Lailatul Syafitri; Evy Diah Woelansari; Museyaroh Museyaroh; Suhariyadi Suhariyadi
CENDEKIA: Jurnal Ilmu Pengetahuan Vol. 6 No. 4 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/cendekia.v6i4.15480

Abstract

Hepatitis B and Hepatitis C are infectious diseases that can be transmitted from pregnant women to their babies during pregnancy, childbirth, or after birth. Screening examinations in pregnant women are important as an early detection effort to prevent vertical transmission. This study aimed to determine the profile of Hepatitis B antibody (HBsAb) and Hepatitis C antibody (Anti-HCV) test results among pregnant women using the immunochromatographic method at the Sidoarjo Community Health Center. This study employed a descriptive research design. The research was conducted at the Laboratory of the Sidoarjo Community Health Center from January to February 2026, involving 40 pregnant women who attended antenatal care (ANC) examinations. Testing was performed using the rapid immunochromatographic method on blood samples collected from the respondents to detect HBsAb and Anti-HCV antibodies. The results showed that, based on age, most respondents were in the 20–35 years age group, accounting for 32 individuals (80%). Based on gestational age, the majority of respondents were in the first trimester, totaling 22 individuals (55%). The HBsAb examination results indicated that 38 respondents (95%) were non-reactive and 2 respondents (5%) were reactive. Meanwhile, the Anti-HCV examination results showed that all 40 respondents (100%) were non-reactive. It is important for pregnant women to undergo Hepatitis B and Hepatitis C screening as a preventive measure to reduce the risk of mother-to-child transmission. ABSTRAK Hepatitis B dan Hepatitis C merupakan penyakit infeksi yang beresiko menular dari ibu hamil kepada bayi selama kehamilan, persalinan, maupun setelah kelahiran. Pemeriksaan skrining pada ibu hamil penting dilakukan sebagai upaya deteksi dini untuk mencegah terjadinya penularan vertikal. Penelitian ini bertujuan untuk mengetahui gambaran hasil pemeriksaan antibodi Hepatitis B (HBsAb) dan antibodi Hepatitis C (Anti-HCV) pada ibu hamil menggunakan metode imunokromatografi di Puskesmas Sidoarjo. Jenis penelitian yang digunakan adalah deskriptif. Penelitian dilakukan di Laboratorium Puskesmas Sidoarjo pada bulan Januari–Februari 2026 dengan jumlah responden sebanyak 40 ibu hamil yang melakukan pemeriksaan antenatal care (ANC). Pemeriksaan dilakukan menggunakan metode imunokromatografi rapid test terhadap sampel darah responden untuk mendeteksi HBsAb dan Anti-HCV. Hasil penelitian menunjukkan bahwa berdasarkan usia, sebagian besar responden berada pada kelompok usia 20–35 tahun sebanyak 32 orang (80%). Berdasarkan usia kehamilan, mayoritas responden berada pada trimester I sebanyak 22 orang (55%). Hasil pemeriksaan HBsAb menunjukkan sebanyak 38 responden (95%) non reaktif dan 2 responden (5%) reaktif. Sementara itu, hasil pemeriksaan Anti-HCV menunjukkan seluruh responden 40 orang (100%) non reaktif. Penting bagi ibu hamil untuk melakukan pemeriksaan Hepatitis B maupun Hepatitis C sebagai upaya pencegahan risiko penularan ibu hamil kepada bayi.
GAMBARAN INSIDENSI HBSAG PADA IBU HAMIL DI PUSKESMAS TULANGAN SIDOARJO Erin Febrianti; Suhariyadi Suhariyadi; Sri Sulami Endah Astuti; Suliati Suliati
CENDEKIA: Jurnal Ilmu Pengetahuan Vol. 6 No. 4 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/cendekia.v6i4.15710

Abstract

Hepatitis B in pregnant women requires attention because infection with the Hepatitis B virus (HBV) can be transmitted from mother to infant and may develop into chronic infection. Therefore, Hepatitis B surface antigen (HBsAg) screening during antenatal care (ANC) is important for early detection and the prevention of mother-to-child transmission. This study aimed to determine the incidence of HBsAg among pregnant women at Tulangan Community Health Center, Sidoarjo, and describe respondents’ characteristics according to age, gestational age, and gravidity. A descriptive study design with purposive sampling was employed, involving 38 pregnant women from a population of 60 women attending ANC services between March and April 2026. HBsAg screening was performed using an immunochromatographic rapid test. Data were analyzed descriptively and presented as frequency distributions and percentages. The results showed that 1 respondent (2.6%) tested reactive for HBsAg, while 37 respondents (97.4%) tested non-reactive. The reactive case occurred in a woman aged 26–35 years, in the second trimester, and classified as secundigravida. In conclusion, the proportion of reactive HBsAg results among the respondents was 2.6%. Routine HBsAg screening during pregnancy is important to support early detection and prevent mother-to-child transmission of hepatitis B. ABSTRAK Hepatitis B pada ibu hamil perlu mendapat perhatian karena infeksi virus hepatitis B (Hepatitis B Virus/HBV) dapat ditularkan dari ibu kepada bayi dan berisiko berkembang menjadi infeksi kronis. Oleh karena itu, pemeriksaan Hepatitis B Surface Antigen (HBsAg) melalui pelayanan antenatal care (ANC) penting dilakukan untuk mendukung deteksi dini dan pencegahan penularan. Penelitian ini bertujuan untuk mengetahui insidensi HBsAg pada ibu hamil di Puskesmas Tulangan, Sidoarjo, serta menggambarkan karakteristik responden berdasarkan umur, umur kehamilan, dan paritas. Penelitian menggunakan desain deskriptif dengan teknik purposive sampling terhadap 38 ibu hamil dari populasi 60 ibu hamil yang menjalani pemeriksaan ANC pada Maret–April 2026. Pemeriksaan HBsAg dilakukan menggunakan metode imunokromatografi dengan rapid test. Data dianalisis secara deskriptif dan disajikan dalam bentuk distribusi frekuensi serta persentase. Hasil penelitian menunjukkan bahwa 1 responden (2,6%) memiliki hasil HBsAg reaktif, sedangkan 37 responden (97,4%) nonreaktif. Kasus reaktif ditemukan pada kelompok umur 26–35 tahun, trimester II, dan secundigravida. Disimpulkan bahwa proporsi hasil HBsAg reaktif pada responden yang diteliti sebesar 2,6%. Pemeriksaan HBsAg secara rutin diperlukan untuk mendukung deteksi dini dan pencegahan penularan hepatitis B dari ibu kepada bayi.
HUBUNGAN ANTARA KADAR ASAM URAT DENGAN KADAR hs-CRP PADA PASIEN PROLANIS HIPERTENSI Sofiyah Sofiyah; Anik Handayati; Suhariyadi Suhariyadi; Edy Haryanto
HEALTHY : Jurnal Inovasi Riset Ilmu Kesehatan Vol. 5 No. 4 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/healthy.v5i4.15860

Abstract

Hypertension is a chronic disease that may increase the risk of cardiovascular disease. Elevated uric acid levels are known to contribute to oxidative stress and inflammatory processes, while high-sensitivity C-reactive protein (hs-CRP) is an inflammatory biomarker used to assess cardiovascular disease risk. This study aimed to determine the relationship between uric acid and hs-CRP levels among hypertensive Prolanis patients at Farmalab Medical Laboratory, Bangkalan Regency. This study employed a quantitative observational analytic design with a cross-sectional approach. Serum samples were obtained from 30 hypertensive Prolanis patients. Uric acid levels were measured using an Homogenus Enzymatic Colometric method, while hs-CRP levels were measured using Fluorescence Immunoassay (FIA). Data were analyzed using the Shapiro-Wilk test and Spearman correlation. The mean uric acid level was 5.913 mg/dL and the mean hs-CRP level was 2.560 mg/L. Spearman correlation analysis showed no significant relationship between uric acid and hs-CRP levels (r = -0.218; p = 0.246). The correlation coefficient indicated a very weak negative relationship. In conclusion, uric acid levels were not significantly associated with hs-CRP levels among hypertensive Prolanis patients. ABSTRAK Hipertensi merupakan penyakit kronis yang berkaitan dengan peningkatan risiko penyakit kardiovaskular dan melibatkan proses inflamasi serta gangguan metabolik. Kadar asam urat yang meningkat diduga berkaitan dengan inflamasi sistemik, yang dapat dinilai melalui pemeriksaan high-sensitivity C-reactive protein (hs-CRP). Penelitian ini bertujuan untuk mengetahui hubungan antara kadar asam urat dan hs-CRP pada pasien Program Pengelolaan Penyakit Kronis (PROLANIS) hipertensi di Laboratorium Medis Farmalab, Kabupaten Bangkalan. Penelitian ini menggunakan desain kuantitatif analitik observasional dengan pendekatan cross-sectional. Sampel penelitian terdiri atas 30 pasien perempuan berusia 40–65 tahun yang dipilih menggunakan teknik purposive sampling. Pemeriksaan asam urat menggunakan metode homogeneous enzymatic colorimetric, sedangkan hs-CRP menggunakan metode fluorescence immunoassay (FIA). Analisis data dilakukan menggunakan uji normalitas Shapiro–Wilk dan korelasi Spearman. Hasil penelitian menunjukkan rata-rata kadar asam urat sebesar 5,913 mg/dL dan hs-CRP sebesar 2,560 mg/L. Uji korelasi Spearman menunjukkan koefisien korelasi sebesar −0,218 dengan nilai p = 0,246. Dengan demikian, tidak terdapat hubungan yang signifikan antara kadar asam urat dan hs-CRP pada pasien PROLANIS hipertensi.
Co-Authors ., Pestariati Abror, Yogi Khoirul Alifiah, Salsabiil Rochman Aliya Ayu Rizqiyah Amalia Putri Khurota Ayyun Andreas Putro Ragil Santoso, Andreas Putro Ragil Anggraini, Anita Dwi Ariyani, Ahna Fitri Asryadin Asryadin Cheppy Juwita Ayuningrum Diah Titik Mutiarawati Edy Haryanto Eka Safitri, Devayanti Eko, Sugeng Endah Astuti, Sri Sulami Endarini, Lully Hanni Erin Febrianti Evy Diah Woelansari Fortuna Aliza Dewi Handayati, Anik Ilma Ainun Nisa Istanto, Wisnu istiqomah istiqomah Istiqomah Istiqomah Juliana Christyaningsih Kanayu Aulia Masardi Kartika Rahayuningsih, Christ Krihariyani, Dwi Kumahartati, Intan Liliek Soetjiatie Luthfi Rusyadi Mamik Mamik Mamik Mamik Melly Nury Sabriyani MINARTI Minarti Minarti Museyaroh, Museyaroh Mutiarawati, Diah Titik Nilasari Indah Yuniati Niswata, Navila Arfianti Novikasari, Caturizkyana Nur Alizah Octarina, Nofita Prasasti, Adelia Gita Purwati Purwati Rahma Yuliana, Putri Rizqiyah, Aliya Ayu Salsabiil Rochman Alifiah Sasongkowati, Retno Shela Yuni Arta Siti Helmyati Sofiyah Sofiyah Sri Utami Suliati Suliati Syamsul Arifin Uswatun Hasanah Vernanda Amyarti Lailatul Syafitri Widiastutik, Dyah Yuninda Zebina Salsabillah