Claim Missing Document
Check
Articles

Found 4 Documents
Search
Journal : INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES

RAGAM ALEL DAN KEKUATAN PEMBEDA LIMA LOKUS MIKROSATELIT DNA AUTOSOM MASYARAKAT SUKU BATAK DI DENPASAR DAN BADUNG I Ketut Junitha; Yossy Carolina Carolina
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 6 (2016): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Five loci of microsatellites DNA autosomal that is: D2S1338, D13S317, D16S539, D18S351, and D19S433 used to determination kind of allele and power of discrimination each locus of Batak ethnic in Denpasar and Badung. DNA sample were amplified used five pairs of primer  D2S1338, D13S317, D16S539, D18S351, and D19S433 loci  in  PCR machine used  PCR mix Invitrogen with annealing temperature  range of 48-56oC. The result of this research was found 54 alleles. The most allele was found on D2S1338 locus, 13 alleles followed by D18S351, 12 alleles, D13S317 and D19S433 locus each 10 alleles and the least one is 8 allele on D16S539 locus. The highest of power of discrimination   that is 0,984 D2S1338 locus followed by D18S351, D19S433, D16S539 and of D13S317 0,973, 0,956, 0,949, and 0,947 respectively. The five loci in this studied had many kind of alleles and high degree of power discrimination value so that loci is useful to forensic purpose for Batak society in Bali.
KARAKTER MORFOLOGI BERAS SEBAGAI PEMBEDA VARIETAS PADI Dicky Frengky Hanas; Eniek Kriswiyanti; I Ketut Junitha
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 7 (2017): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJLFS.2017.v07.i01.p04

Abstract

This study aims to determine the morphological character of white, red and black rice as a differentiator ofrice varieties. This research uses 10 rice samples which are 6 local rice of Kupang, 2 varieties of local superior riceand 2 Italian rice. The method used is direct observation of both qualitative and quantitative characteristics of rice.Long grain size of rice is a character that can indicate the distinguishing of local rice, domestic superior and ricefrom abroad. Character of rice morphology is a character very easily observed in knowing the difference of ricevarieties. Character of rice morphology are easily characters observed in knowing the difference of rice varieties.Characters difference can be used to determine the adulteration of rice mixed.
IDENTITAS GENETIK KETURUNAN SOROH PANDE DI KECAMATAN SERIRIT, BULELENG BERDASARKAN DNA MIKROSATELIT KROMOSOM Y I Wayan Arya Mahardika; I Ketut Junitha; Inna Inna Narayani
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 6 (2016): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJLFS.2016.v06.i01.p01

Abstract

The Balinese clans embraced patrilineal system.They divided into groups of soroh or clans which means respect for ancestors. Pandeclan is a unique soroh which comes from ancestors who have expertise in memande. The genetic identityof this clan in Bali is unknown.Therefore the aim of this study is to find out the genetic identity from the varieties of allelic diversity which found on Pande community inside and outside Seririt using microsatellite DNA analysis of Y chromosome, namely DYS19, DYS390, DYS393, and DYS395. Resultsof this study have foundthat in Pande clan there are 21 varieties of allelic diversity and 25 haplotypes with the average heterozygosity of four loci is 0.72 ± 0.03. The dendogram indicates that Pande clanfrom district of Seririt did not cluster based on it’s dadia.
IDENTIFIKASI ALEL DAN KEKUATAN PEMBEDA EMPAT LOKUS DNA MIKROSATELIT KROMOSOM-Y PADA MASYARAKAT KLAN PANDE DI BALI UNTUK KEPENTINGAN FORENSIK I Ketut Junitha; I Made Sara Wijana
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 7 (2017): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJLFS.2017.v07.i01.p01

Abstract

This research was conducted to know alleles variation and power of discrimination of four pair of primer: DYS19, DYS390, DYS393 and DYS395 of male Pande clan.  Epithelial cell mucus was collected  from  59 male of Gainer, Klungkung, Badung regencies and Denpasar city. Phenol chloroform method was used to extraction DNA from epithel cells sample.  Four primer pair was used to amplify DNA samples in PCR machine on 52-55oC annealing temperature. Amplicon  were running on PAGE 10% and visualized  with silver nitrate staining.  The DNA typing were conducted  to determinate of alleles size of amplicon  with plotting migration distance of amplicon on semi-log pepper.  Genetic diversity and power of discrimination was calculated used Microsoft Exell program.  The result of this research showed that 29 alleles were found range of 6-9 with mean 7.25 per locus. The genetic diversity is high category (0.739 ± 0.003, the highest diversity on DYS 390 locus  is 0.809 ±  0,004 followed by DYS395, DYS393 are 0,793 ±  0.004 and 0,720 ± 0.005 and the small one is 0.633 ±  0.003 on DYS19 locus. The power of discrimination (PD) of all loci are high category with average value is 0.892, because of / for this reason  all of loci usfulnes for  forensic purpose on pande clan in Bali.