Article Per Year (5 Year)
p-Index From 2021 - 2026
2.93
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES METAMORFOSA Journal of Biological Sciences Jurnal Farmasi Udayana INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Jurnal Biologi Udayana Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Kajian Bali Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Journal of Tropical Ethnobiology International Journal of Applied Science and Sustainable Development (IJASSD) International Journal of Scientific Multidisciplinary Research (IJSMR) Metamorfosa: Journal of Biological Science Open Access DRIVERset
I Ketut Junitha
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mechanical Engineering Medicine & Pharmacology Social Sciences

Published : 40 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 6 Documents
Search
Journal : METAMORFOSA Journal of Biological Sciences

 1 
RAGAM ALEL DNA MITOKONDRIA MASYARAKAT SOROH PANDE DI BALI DENGAN METODE PCR-RFLP Ni Putu Senshi Septiasari; I Ketut Junitha; Ni Nyoman Wirasiti
Metamorfosa: Journal of Biological Sciences Vol 4 No 2 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i02.p12

Abstract

Masyarakat Bali mengelompokkan diri berdasarkan soroh atau garis keturunan tetapi bukti genetik pengelompokkan tersebut belum diketahui. Salah satu soroh/ klan yang memiliki persebaran yang luas di seluruh Bali adalah soroh/ klan Pande. Penelitian ini dilakukan untuk mengetahui ragam alel DNA mitokondria masyarakat soroh Pande di Bali dengan metode Polymerase Chain Reaction-Fragment Length Polymorphism (PCR-RFLP) yang berguna untuk kepentingan forensik. Sampel sel epitel mukosa mulut diambil dengan cara purposive sampling yang terdiri dari 35 sampel warga Pande. Tahapan penelitian meliputi ekstraksi DNA dengan metode fenol-kloroform yang telah dimodifikasi, amplifikasi DNA pada daerah D-loop DNA mitokondria (mtDNA), digesti produk PCR dengan lima macam enzim restriksi dan elektroforesis dengan menggunakan gel agarose 1 % dan PAGE 10%. Penelitian ini mendapatkan sembilan ragam alel. Digesti dengan enzim Hae III, Hinf I, Mbo I dan Dde I mendapatkan masing-masing dua tipe pemotongan sedangkan enzim Hind III tidak memiliki situs pengenalan pada daerah D-loop DNA mitokondria. Enzim Hind III tidak dapat digunakan untuk membedakan profil DNA antar individu karena bersifat monomorfik.
Perubahan Golongan Darah Dan Identifikasi Bakteri Yang Berperan Dalam Merubah Golongan Darah Bagas Susilo; I Ketut Junitha; Yan Ramona
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p13

Abstract

Blood is one of the most important component of human body. In a case of crime, blood found at the site can be used as an evidence to track back the victim or the person who did such criminal. The main objective of this research were to investigate the time needed for the convertion of blood types and to identify bacterial species with capability to convert blood types. This research was conducted at Forensic Laboratory and Microbiology Laboratory, Faculty of Mathematics and Science, Udayana University. The methods used for detection of blood types and identification of Bacterias were elution method and vitex method, respectively. The results showed that blood type A on iron surface and ceramic media was changed into fake O type on day 25, while blood types of B and AB on ironic media were converted into fake type of O after 30 days of exposure. Two species bacteria (Rhizobium radiobacter and Serratia ficaria) with ability to convert blood type were identified in this research. Key Word: Rhizobium radiobacter, Serratia ficaria, changes of blood types
Perubahan Golongan Darah Berdasarkan Pengaruh Waktu dan Mikroorganisme Yang Berperan Muhamad Masyrur; I Ketut Junitha; Meitini W. Proborini
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p05

Abstract

Blood is one of the most important evidence in the forensic analysis. Blood has a special characteristic or identity of the owner. Blood can be analyzed blood type to know the owner of the blood. But in the course of time, the blood will be degraded so it will be change of blood type into blood type O. One of the contributing factors is the presence of microorganisms that it grow in the blood. The material of this research is the human blood of blood type A, B, AB and O from 4 (four) respondents of 3 (three) males and 1 (one) female who are 20 to 40 years old. Each sampel of 150 µL was dropped on sterile gauze size 2 x 6 cm, then it was dried. Sample were stored at room temperature (± 30 oC) for 0, 30, 60, 90 and 120 days. Sampel was tested for blood type using absorption elution method based on the work instructions from The Police Forensic Laboratory of Denpasar Branch in 2016. The results of this research indicate that the human dried blood stored on sterile gauze media for 30 and 60 days has not changed its blood type. Storage of the human dried blood for 90 and 120 days, it have changed blood type with found antigen A or B that was not detected back through absorbtion elution analysis on blood group A, B and AB. This research found 9 species of fungi and 7 species of bacteria that they grow on the blood samples. Species of Fungi and bacteria that have high ability to degrading all blood group (A, B and AB) are Acromonium carticola, Alternaria alternata, Aspergillus flavus, Aspergillus niger, Penicillium sp and Bacillus coagulans. While species of fungi and bacteria that have high ability to degrading blood group B or AB are Fusarium sp, Trichoderma harzianum, Trichoderma viridae, Bacillus sp and Staphylococcus cohnii.
STRUKTUR GENETIK DAN RAGAM ALEL TIGA GENERASI MASYARAKAT BALI BERDASAR LIMA LOKUS PENANDA DNA MIKROSATELIT AUTOSOM Ayu Saka Laksmita; I Ketut Junitha; Ni Luh Watiniasih
Metamorfosa: Journal of Biological Sciences Vol 2 No 2 (2015)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2015.v02.i02.p02

Abstract

This research was aimed to find out the genetic structures of three generations of Balinese population, in order to determine the best loci used for paternity testing among this population, and observed the mutation rate of these loci. The DNA samples were taken from the epithelium cell of 25 families which were collected from the children, father, mother, grandfather and grandmother of the children, from both mother and father sides (family with three generations). The DNA was extracted in Phenol-Chloroform method with modifications. DNA amplification was conducted in PCR method using pairs of primer 5, namely: FGA, D18S51, D2S1338, TPOX, and D16S539, and its products were electrophoresed and visualized in 10% of PAGE, stained in silver nitrate. The genetic structures of the three family generations showed 30 variants with different frequencies in each locus. The highest heterozygosity value was detected in FGA (8 alleles), then followed by D18S51 (7 alleles), TPOX (6 alleles), D16S539 (5 alleles), and the lowest was in D2S1338 (4 alleles). The highest value of heterozigosity and Power of Discrimination were found in FGA, followed by TPOX, D18S51, D2S1338, and the lowest was in D16S539. Therefore, it can be concluded that out of five loci tested, 4 of them can be recommended to be used for paternity testing of Balinese population, except D16S539. Keywords: Paternity Testing, DNA Microsatellite, three generations, heterozygosity, Power of Discrimination
PENGARUH MUTAGEN KIMIA SODIUM AZIDA TERHADAP MORFOLOGI TANAMAN CABAI BESAR (Capsicum annuum L.) Ni Kadek Yunita Sari; Made Pharmawati; I Ketut Junitha
Metamorfosa: Journal of Biological Sciences Vol 1 No 1 (2012)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aim of this research was to examine the effects of sodium azide at concentration of 1 mM, 2 mM, 3 mM, 4 mM and 5 mM on the morphological characters of Capsicum annuum L. The method used for the observation of morphological characters was direct observation by measuring plant height, leaf length and width as well as counting the number of leaves. The results showed that effect of sodium azide at 3 mM caused an increase in plant height, while concentration of 1 mM, 2 mM, 4 mM dan 5 mM caused a decrease in plant height. Similar trends were found for number of leaf. Leaf length and width tended to increase in sodium azide treatments.
Kuantitas Dan Kualitas DNA Hasil Ekstraksi Dari Bercak Darah Pada Pisau Pasca Paparan Sinar Ultraviolet Dan Matahari Putri Arie Prasetyoningrum; I Ketut Junitha; Dwi Ariani Yulihastuti
Metamorfosa: Journal of Biological Sciences Vol 10 No 1 (2023)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2023.v10.i01.p19

Abstract

Examination of Deoxyribonucleic Acid is the primary identification method in forensic cases. Blood stains found at the scene of a crime are often affected by various environmental factors, one of which is ultraviolet light from the sun. Purpose of this study is to compare the quantity and quality of DNA extracted from blood stains on the blade after exposure to ultraviolet light and the sun for 0, 15 and 30 days. The method used is a factorial design: light source (UVA, indirect sunlight and direct sunlight); and duration of treatment (0, 15 and 30 days). Blood stains are made by dripping a 50?L of human blood on one side of the blade and then given treatment. DNA analysis includes: extraction with Chelex 5%; DNA quantity and quality test with SimpliNano spectrophotometer; total DNA quality test with agarose gel electrophoresis; and PCR. Quantitative data analysis using Univariate test followed by Duncan test. The results showed that the quantity of DNA in samples exposed to UVA and sunlight (directly) increased on the 15th day due to DNA fragmentation while samples exposed to sunlight (indirectly) decreased the quantity of DNA with the duration of treatment. DNA quality in the form of DNA purity produces an extract of DNA that is not pure. The total DNA quality with agarose gel electrophoresis on all samples showed that the longer the treatment (0, 15 and 30 days) resulted in a dimmer or thinner band of DNA luminescence with smears.
Co-Authors A.A. Gde Lanang Adelina Arifiani Purnomo Agriani Dewinta Anastesya Hartika Nur Saputri Ayu Saka Laksmita W Bagas Susilo Bagus Alit, Ida Bambang Suryobroto Bram Setyadji Budiningsih, Desak Nyoman Cakra Diarsa Chandra Yowani Christian Subagya Gunardi Dewa Ayu Sri Ratnani Dwi Ariani Yulihastuti ENIEK KRISWIYANTI Eswaryanthi Kusuma Yuni Fita Efi Rafelina Siallagan Graciella Stevani Gulo Gusti Ayu Putri Arnila Hanas, Dicky Frengky I Gde Suryadi I Gede Yeyen Suharta I Gusti Agung Eka Saraswati I K. Sukardika I Ketut Sundra I Made Sara Wijana I NYOMAN MANTIK ASTAWA I Wayan Arya Mahardika Ida Ayu Manik Damayanti Ida Bagus Alit Ida Bagus Bajing Agastya Ida Bagus Made Suaskara IMA Gelgel Wirasutha Inna Narayani IRIANI SETYAWATI Kezia Artanauli Purba Lucia Emy Octavia MADE PHARMAWATI Maya Agustina Maya Agustina Meitini Proborini Wahyuni Muhamad Masyrur NI KADEK YUNITA SARI Ni Luh Putu Ria Puspitha Ni Luh Putu Rika Sugiantari Ni Luh Watiniasih Ni Made Puspawati Ni Made Rai Suarni Ni Made Suartini Ni Made Suartini Ni Nyoman Wirasiti Ni Putu Puniari Eka Putri, Ni Putu Puniari Eka Ni Putu Putri Wulandari Putri Arie Prasetyoningrum Putri Karenina Siregar Putu Rosi Setyari SANG KETUT SUDIRGA Septiasari, Ni Putu Senshi Tyara Tyara Victor Peter Poli Yan Ramona Yossy Carolina Carolina YOSSY CAROLINA UNADI Yulia Mirayanti