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All Journal UNEJ e-Proceeding Nusantara Medical Science Journal Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Jurnal Ilmu Alam dan Lingkungan Indonesian Journal of Obstetrics & Gynecology Science Alami Journal (Alauddin Islamic Medical) Journal Media Kesehatan Politeknik Kesehatan Makassar Jurnal Biomedika Jurnal Riset Kesehatan Poltekkes Depkes Bandung Celebes Health Journal Indian Journal of Forensic Medicine & Toxicology JUMANTIK (Jurnal Ilmiah Penelitian Kesehatan) Majalah Anestesia & Critical Care (MACC)

Firdaus Hamid

Unknown Affiliation

Author-ID : 2051869

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Molecular Detection of VEB and OXA-23 Resistance Gene in Pseudomonas Aeruginosa Isolates at Dr. Wahidin Sudirohusodo Hospital Makassar Andi Zsazsa Rafiatul Mukhlis; Rizalinda Sjahril; Firdaus Hamid
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 16, No 1 (2022): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33533/jpm.v16i1.4144

Pseudomonas aeruginosa is a common gram-negative pathogen in nosocomial infections in immune-compromised patients. It exhibits high rates of intrinsic resistance to many classes of antibiotics, especially beta-lactam antibiotics. Production of extended-spectrum beta-lactamase (ESBL) and genes belonging to the carbapenem-hydrolyzing class D subgroup β-lactamases (CHDL) are a problem for increasing antibiotic resistance worldwide. This study aimed to identify P. aeruginosa containing the VEB and OXA-23 genes. Eighty-five clinical isolates of P. aeruginosa from various clinical samples were identified and tested for antimicrobial susceptibility using VITEK 2 compact. VEB and OXA-23 genes were detected using the Polymerase Chain Reaction (PCR) method. The PCR results revealed that 13 (15.3%) of P. aeruginosa isolates were positive OXA-23 gene, but no isolate positive for the VEB gene in P. aeruginosa isolates. The study results demonstrated the spread of the OXA-23 gene in P. aeruginosa isolates at Dr. Wahidin Sudirohusodo Hospital Makassar.

PENGGUNAAN MULTIPLEKS POLYMERASE CHAIN REACTION (PCR) DALAM MENDETEKSI JAMUR DERMATOFIT Aan Yulianingsih Anwar; Rizalinda - Sjahril; Firdaus - Hamid
Media Kesehatan Politeknik Kesehatan Makassar Vol 14, No 1 (2019): Media Kesehatan
Publisher : Politeknik Kesehatan Makassar

Dermatofitosis adalah salah satu jamur yang terdiri dari tiga genus: Epidermophyton, Trichophyton dan Microsporum. Tujuan penelitian ini untuk mengidentifikasi jenis jamur dermatofit dengan metode Multipleks PCR yang ditemukan pada penderita dermatofitosis di Kota Makassar. Penelitian observasi laboratorium dengan menguji 50 sampel yang diperoleh dari beberapa klinik dan sekolah dasar di Makassar. Hasil penelitian menunjukan bahwa Microsporum spp. terbanyak teridentifikasi (54%). Kami menyarankan teknik Multipleks PCR ini digunakan untuk konfirmasi jenis dermatofit sehingga pengobatan dapat lebih cepat dan tepat.

Deteksi gen pneumolysin (ply) Streptococcus pneumoniae pada Sampel Klinis Usia Lanjut secara Kultur dan PCR Muthmainnah Muthmainnah; Mochammad Hatta; Muh. Nasrum Massi; Firdaus Hamid; Irawati Djaharuddin; Eddyman W Ferial; Andi Alfian Zainuddin; Mustika Hutabarat Sari
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 12 No 1 (2020): Jurnal Riset Kesehatan Poltekkes DepKes Bandung
Publisher : Poltekkes Kemenkes Bandung

ABSTRACT Streptococcus pneumoniae, a gram-positive bacterium of normal flora but can be a pathogenic bacterium that causes pneumonia if the immune system declines. Community pneumonia ranks fourth and top ten diseases treated per year. Populations that are susceptible to pneumonia are children aged less than 2 years, old age. The Purpose aim to detect ply genes in sputum with suspected pneumonia in elderly as a virulence factor of S. pneumoniae by culture and PCR. This research method is a descriptive study to detect the virulence factor of the S. pneumoniae ply gene in the sputum of patients with suspected pneumonia in the elderly with the culture and PCR methods. Conventionally (culture method) obtained 1 sample from 56 positive samples of S. pneumoniae gram positive bacteria on the molecular method (PCR) there were 7 positive samples of S. pneumoniae bacteria from 56 samples. In detecting ply genes in sputum with suspected pneumonia in elderly as a virulence factor from S. pneumoniae bacteria were culturally detected 1 positive sample from 56 samples and by PCR method detected 7 positive samples from 56 samples. In detecting ply genes using culture and PCR it can be said that the PCR method is more accurate, fast and precise in detecting the S. pneumonia gene in sputum samples with suspected pneumonia in elderly.

Hubungan Pola Makan Dan Self Image Dengan Status Gizi Remaja Putri SMA (15-18 Tahun) Wahyuni Alinengsih; Veni Hadju; Aminuddin Aminuddin; Firdaus Hamid; Sharvianty Arifuddin; Burhanuddin Bahar
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 12 No 1 (2020): Jurnal Riset Kesehatan Poltekkes DepKes Bandung
Publisher : Poltekkes Kemenkes Bandung

Objective: This study aims to study the relationship between eating patterns and self-image with nutritional status in high school girls (15-18 years) in Polongbangkeng Utara District, Takalar Regency. Method: A cross sectional study design with 170 students taken by purposive sampling technique in girls aged 15-18 years in SMA 6 and 11 in Polongbangkeng Utara Takalar Regency using questionnaires, anthropometry, and weighing levels of fat, in the month September-October 2019. This study uses analysis using the Chi Square Test. Results: The results of the analysis showed differences between adolescents' age, education age and retirement, adolescent nutritional status, results of statistical tests on diet with nutritional status based on BMI (p = 0.133> α = 0.05), self-image with nutritional status based on BMI (p = 0.86> α = 0.05), diet with nutritional status based on fat content (p = 0.333> α = 0.05), between self-image and nutritional status based on fat content (p = 0.869> α = 0.05), and between BMI with fat content (p = <0.001 <α = 0.05).

Analisis Resiko Kejadian Akut Rekuren Demam Tifoid dan Hubungannya dengan Kadar Protein Nucleotide Binding Oligomerization Domain 2 (NOD2) Sri Wahyuni; Mochammad Hatta; Firdaus Hamid; Rosdiana Natzir; Ahyar Ahmad; Burhanuddin Bahar; Ade Rifka Junita; Ressy Dwiyanti; Nur Indah Purnamasari; Muhammad Reza Primaguna; Muhammad Sabir
Jurnal Ilmu Alam dan Lingkungan Vol. 12 No. 2 (2021): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jal.v12i2.17585

Typhoid fever is an infectious disease caused by Salmonella typhi bacteria and is endemic. The NOD2 gene is one of the host susceptibility genes in people with typhoid fever. NOD2 acts as an intracellular receptor that binds to the muramyl dipeptide ligand derived from bacterial peptidoglycan. The purpose of this study was to determine the levels of NOD2 protein in Acute Recurrent of Typhoid Fever (ARTF), typhoid fever (TF) patients, and healthy people (HP). Enzyme-linked Immunosorbent Assay (ELISA) was used to analyze NOD2 levels. The data analysis used was the student t-test. A significant difference of NOD2 level was found between the ARTF and TF group compared HP group (p

The Analysis of Vitamin D Receptor Protein on Salmonella typhi infection in acute recurrent cases in endemic area in Eastern Indonesia Fatmawati Annisa Syamsuddin; Mochammad Hatta; Firdaus Hamid; Rosdiana Natzir; Ahyar Ahmad; Burhanuddin Bahar
Biomedika Vol 14 No 2 (2021): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v14i2.1298

The host susceptibility mechanisms such as Vitamin D Receptor (VDR) is involved in the modulation of macrophage function and may possibly correlate with immunity disease including the severity of typhoid fever symptoms. The study aimed to assess the VDR Protein expression in the serums of recurrent acute typhoid fever (RATF) patients and compares it with typhoid fever (TF) patients, and healthy persons (HP). The study employed 30 RATF patients and 30 TF patients selected from primary health centres and hospitals in Eastern Indonesia as the endemic area. All the samples were obtained from several health centers in South Sulawesi, Southeast Sulawesi, Central Sulawesi, East Kalimantan and Papua and then collected in the sample bank Biology Laboratory of Molecular Immunology, Faculty of Medicine, Hasanuddin University. As a comparison, 30 samples of healthy persons were also selected from the Blood Transfusion Unit in Makassar, South Sulwesi Indonesia. The profile of VDR Protein was analyzed with Enzyme-linked Immunosorbent Assay (ELISA). VDR protein content data on RATF and TF were designed according to completely randomized design T test. Subsequently, it correlated to Pearson correlation to determine the interaction between Widal titre and VDR protein levels. A comparison between Widal titre and VDR Protein level was also made to identify the correlation. It was found that the mean of VDR protein expression of RATF was 13,44 ng/mL, the mean of VDR protein expression of TF was 24,88 ng/mL, and the mean of VDR protein expression of HP was 43,49 ng/mL. The correlation results between RATF-TF Widal titre and VDR protein level indicated a negative correlation with p-value of 0,004. There were significant differences in the VDR expression in the RATF, TF, and HP. RATF VDR expression lower than TF and HP and there was also a correlation between Widal titre with VDR Protein expression.

Isolasi Antimikroba Baru dari Bakteri Tanah Evi Damayanti; Firdaus Hamid; Rizalinda Sjahril
JUMANTIK (Jurnal Ilmiah Penelitian Kesehatan) Vol 7, No 2 (2022)
Publisher : Prodi Kesehatan Masyarakat Fakultas Kesehatan Masyarakat UIN Sumatera Utara

Antimicrobial resistance has become a problem and a threat to health both in Indonesia and in the world. Resistance is the ability of bacteria to neutralize and weaken the action of antibiotics. The aim of this study was to isolate a novel antimicrobial from soil bacteria. The method used in this research is exploratory. The test bacteria used were Staphylococcus aureus and Pseudomonas aeruginosa. The results showed that there were two isolates that had zones of inhibition against the test bacteria staphylococcus aureus, namely isolates T2.2 and T2.18. The results of the first antimicrobial test for isolate T2.2 showed that the inhibition zone formed was 14.05 mm and isolates T2.18 the inhibition zone formed was 11.96 mm. The results of the second antimicrobial test were carried out by treating the unheated T2.2 isolate which showed an inhibition zone formed of 15.53 mm and the heated one 15.46 mm, while the unheated T2.18 isolate showed an average inhibition zone of 12.46 mm and heated 12.21 mm. The conclusion of this study is that the inhibition zone formed on isolates T2.2 and T2.18 is still relatively small and both isolates are spesies of Pseudomonas aeruginosa.

Effect of Stromal Vascular Fraction and Platelet-Rich Plasma Combination on Polymorphonuclear Cells in Wistar Rats Anal Trauma Model Erwin Chandra; Sulmiati Sulmiati; Nita Mariana; Fonny Josh; Sachraswaty Rachman; Firdaus Hamid
Nusantara Medical Science Journal Volume 7 Issue 2, July - December 2022
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.v7i2.20846

Introduction: By relying on polymorphonuclear (PMN) cells, stromal vascular fraction (SVF) and platelet-rich plasma (PRP) contribute significantly in the healing process. Through anal trauma model, this study examined the effect of SVF and PRP combination on PMN counts in vivo. Method: Three groups of twenty-eight eligible Wistar rats anal trauma model randomly assigned to receive SVF and PRP combination (Group A), normal saline (Group B), or healthy controls (Group C). On days 1, 7, and 14, we examined at PMN cell counts in Groups A and B. Group C were sacrificed early to acquire baseline data on PMN cells. Results: On the first day, Group A had more PMN cells than Group B, but this difference faded by days 7 and 14. On day 1, the test revealed a significant increase in PMN cells (p=0.002). Conclusion: Combining SVF and PRP can improve PMN cells' acute phase of anal damage repair in vivo

Efek Tindakan Transcranial Magnetic Stimulation (TMS) terhadap Derajat Nyeri dan Kadar Beta-Endorfin Serum pada Pasien Nyeri Kanker Payudara Noor Ramadhaniah; Nur Surya Wirawan; A.M. Takdir Musba; Firdaus Hamid; Muh. Ramli Ahmad; Alamsyah Ambo Ala Husain; Madonna Damayanthie Datu
Majalah Anestesia & Critical Care Vol 41 No 1 (2023): Februari
Publisher : Perhimpunan Dokter Spesialis Anestesiologi dan Terapi Intensif (PERDATIN) / The Indonesian Society of Anesthesiology and Intensive Care (INSAIC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55497/majanestcricar.v41i1.274

Latar Belakang: Nyeri adalah keluhan yang sering dirasakan oleh pasien kanker. Benjolan tanpa rasa sakit adalah gejala pertama yang dirasakan pada kanker payudara. Pada stadium lanjut, pasien kanker payudara mengalami rasa sakit yang sangat menyiksa karena keterlibatan struktur didalamnya, selain itu nyeri juga dapat disebabkan oleh pengobatan kanker itu payudara itu sendiri. Stimulasi Magnetik Transkranial (SMT) merupakan metode baru stimulasi otak secara non-invasif untuk manajemen nyeri terkait kanker. Pada nyeri kronik, SMT terbukti menurunkan Visual Analogue Score (VAS) dan meningkatkan serum beta-endorfin. Tujuan: Mengetahui efek tindakan SMT terhadap intensitas nyeri dan kadar beta-endorfin serum serta korelasinya pada pasien nyeri kanker payudara. Subjek dan Metode: Penelitian ini merupakan penelitian prospective randomized controlled trial dengan desain pretest-posttest control group. Sampel terdiri dari 2 kelompok, yaitu kelompok P1 (kelompok dengan intervensi SMT) dan P2 (kelompok kontrol) dengan jumlah sampel masing-masing 20 orang yang memenuhi kriteria inklusi. Dilakukan penilaian Numeric Rating Scale (NRS) dan pengambilan darah untuk pemeriksaan kadar beta endorfin serum sebelum dan sesudah intervensi. Data dianalisis menggunakan uji statistik Mann-Whitney dan Wilcoxon, serta uji korelasi dengan uji Spearman. Hasil: Penurunan NRS lebih besar pada kelompok P1 dibandingkan kelompok P2 dengan nilai p<0.001 dan terjadi peningkatan kadar beta endorfin serum pada kelompok P1 dibandingkan dengan kelompok P2 dengan nilai p<0.001, hal ini memiliki korelasi kuat (r=0.630; p<0.001) Simpulan: Tindakan SMT menyebabkan penurunan intensitas nyeri dan meningkatkan kadar beta endorfin serum pada pasien nyeri kanker payudara dan ditemukan korelasi antar kedua variabel ini. Kata kunci: nyeri kanker, SMT, NRS, beta endorfin

Positive Blood Culture Results Predominantly Unrevealed among Adults Patients with Sepsis Hursepuny, Valentine; Baedah Madjid; Firdaus Hamid; Mochammad Hatta; Muh. Nasrum Massi; Rizalinda Sjahril; Yoeke Dewi Rasita; Lisa Tenriesa; Andi Rofian Sultan
Alami Journal (Alauddin Islamic Medical) Journal Vol 8 No 1 (2024): JANUARY
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/alami.v8i1.41777

Sepsis is increasing every year and can be life-threatening. In the diagnosis of sepsis, it is challenging to identify the causative agent of infection. This research aims to determine the proportion of positive blood culture in sepsis patients at Hasanuddin University Hospital, Makassar. Blood cultures were collected from 98 adult patients with sepsis manifestations. This research design is cross-sectional with a descriptive observation approach. The data analysed in descriptive statistics were utilised to summarise patient demographics, treatments, and outcomes. Thirteen of 98 samples were positive for microbial pathogens (13.26%). The elderly group had the highest percentage (76.5%); eighty-seven patients (88.77%) were treated with antibiotic therapy prior to blood culture collection. Cephalosporins are the most administered antibiotics (72.4%). Administration of empirical therapy prior to blood culture collection may decrease the sensitivity of culture results. The culture of the infectious focus should be done to assist in the consideration of antimicrobial treatment.

Co-Authors A. Kaisar Adiwijaya Putra A.M. Takdir Musba Aan Yulianingsih Aan Yulianingsih Anwar Ade Rifka Junita Agussalim Bukhari Ahyar Ahmad Ahyar Ahmad Alamsyah Ambo Ala Husain Alfian Zainuddin Alfina Baharuddin Aminuddin Aminuddin Andi Alfian Zainuddin Andi Alfian Zainuddin Andi Asadul Islam Andi Ihwan Andi Meutiah Ilhamjaya Andi Nursanty Padjalangi Andi Rofian Sultan Andi Zsazsa Rafiatul Mukhlis Arif Santoso Arif Santoso Arsyi Adliah Anwar Awaluddin Awaluddin Baedah Madjid Besse Afriana Arif Bukhari, Agussalim Burhanuddin Bahar Burhanuddin Bahar Burhanuddin Bahar Burhanuddin Bahar Dario A Nelwan Dianawaty Amiruddin Eddy Hartono Eddyman W Ferial Efendi Lukas Elizabeth Catherine Jusuf Erwin Chandra Evi Damayanti Faridha Ilyas Fatmawati Annisa Syamsuddin Febyan Rasmin Kotto Fonny Josh Hardjono Sumarlie Hursepuny, Valentine Irawati Djaharuddin Irawaty Djaharuddin Junus Asiu Bulu Baan Kenny Edward Yap Khairuddin Djawad Lisa Tenriesa M. Sabir Madonna Damayanthie Datu Misriyani Misriyani Mochammad Hatta Mochammad Hatta Mochammad Hatta Muh. Nasrum Massi Muh. Ramli Ahmad Muhammad Nassrum Massi Muhammad Reza Primaguna Mustika Hutabarat Sari Mustika Sari Hutabarat Nasrudin Andi Mappeware Nita Mariana Noor Ramadhaniah Novita Prastiwi Nur Indah Purnamasari Nur Surya Wirawan Nusratuddin Abdullah Priady Wira Prasetia Ressy Dwiyanti Rizalinda Sjahril Rizalinda Sjahril Rizalinda Sjahril Rizalinda Sjahril Rizalinda Sjahril Rosdiana Natzir Rossana Agus Sachraswaty Rachman Samrichard Rambulangi Sharvianty Arifuddin Siska Nuryanti Siti Fitrah Irmiyanti Hamid Solly Aryza Sri Wahyu Sri Wahyuni Sulmiati Sulmiati Suryani As’ad Syahruni Syahrir TOKA, WAHYUNITA DO Veni Hadju Wahyuni Alinengsih Yoeke Dewi Rasita Yulfa Rizki Amita

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