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Journal : Cropsaver : Journal of Plant Protection

Potency of Bacillus subtilis and Lysinibacillus Mixed With Silica Nano Particles and Carbon Fiber in Controlling Potato Leaf Blight Disease [Phytophthora infestans (Mont.) de Bary] Hersanti Wartono; Fitri Widiantini; Kirana Sonya Harviana
CROPSAVER Vol 2, No 2 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (406.821 KB) | DOI: 10.24198/cropsaver.v2i2.22473

Abstract

Leaf blight disease caused by Phytohthora infestans is one of the major diseases on potato plant. To control the disease farmers usually use synthetic fungicides. However, the excessive use of synthetic fungicides may cause negative impacts on the environment. One of the alternative control methods which is more environtmentally friendly is the use of antagonistic microbes. In this experiment, Bacillus subtilis and Lysinibacillus sp. were formulated with carbon fiber as a carrier and enriched with nano silica as a micro nutrient, to find out the ability of the bacteria in suppressing leaf blight disease on potato plant. The experiment was carried out in the experimental field in Lembang, Bandung Barat, Jawa Barat. The experiment was arranged in the randomized block design consisted of nine treatments and four replications. Density of B.subtilis and Lysinibacillus sp. were 1011 cfu/ml. The formulation was applied 3 times, on planting, 2 weeks and 4 weeks after planting. The results showed that the application of B. subtillis and Lysinibacillus sp. were able to suppress leaf blight disease, with the disease inhibition 36,6% and 32,7% respectively. The combination treatment of B. subtilis in silica nano and carbon fiber was able to suppress leaf blight disease on potato plant higher compared to the application of bacteria without nano silica and carbon fiber, with the disease inhibition up to 51,2%.
The Ability of Nano Chitosan and Bacillus subtilis (Ehrenberg) Cohn to Suppress the Growth of Alternaria porri (Ellis) Ciferri Al Farisy, Muhammad Restu; Meliansyah, Rika; Susanto, Agus; Hersanti, Hersanti
CROPSAVER Vol 7, No 2 (2024)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v7i2.58185

Abstract

Purple blotch disease, caused by the fungus Alternaria porri (Ellis) Ciferri, poses a significant threat to shallot crops. Environmentally friendly control methods, such as applying nano-sized chitosan and Bacillus subtilis (Ehrenberg) Cohn, offer promising alternatives. This study aims to determine the effective concentrations of nano chitosan and B. subtilis, individually and in combination, to suppress the growth of A. porri in-vitro. The research was conducted from March to June 2024 at the Phytopathology Laboratory and Plant Protection Biotechnology Laboratory, Department of Pests and Plant Diseases, Faculty of Agriculture, Universitas Padjadjaran. A completely randomized design was employed, consisting of nine treatments with three replications each: a control treatment, B. subtilis 107 CFU/ml, 50 ppm nano chitosan, 100 ppm nano chitosan, 200 ppm nano chitosan, B. subtilis 107 CFU/ml + 50 ppm nano chitosan, B. subtilis 107 CFU/ml + 100 ppm nano chitosan, B. subtilis 107 CFU/ml + 200 ppm nano chitosan, and 80% mancozeb. The results indicated that nano chitosan at a concentration of 100 ppm was the most effective single treatment which could suppress the growth of A. porri colonies by 94% and inhibit conidia germination by 95%. Meanwhile, B. subtilis 107 CFU/ml + 100 ppm nano chitosan is the most effective mixed treatment which could suppress the growth of A. porri colonies by 84% and inhibit conidia germination by 90%.