Article Per Year (5 Year)
p-Index From 2020 - 2025
2.56
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biogenesis: Jurnal Ilmiah Biologi Jurnal Hortikultura AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Entomologi Indonesia Makara Journal of Science
Abdjad Asih Nawangsih
Department Of Plant Protections, Faculty Of Agriculture, Institut Pertanian Bogor, Jalan Agatis Kampus Dramaga Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

Published : 32 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 32 Documents
Search

 1   2   3   4 
Screening of Liliaceae Rhizosphere Actinomycetes as Biological Control Agents of Fusarium oxysporum f. sp. cepae Eka Wijayanti; Abdjad Asih Nawangsih; Efi Toding Tondok
Jurnal Fitopatologi Indonesia Vol 17 No 6 (2021)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.17.6.225-232

Abstract

Penyakit moler yang disebabkan oleh Fusarium oxysporum f. sp. cepae merupakan salah satu penyakit penting pada tanaman bawang merah. Aktinomiset memiliki potensi sebagai agens pengendali hayati F. oxysporum f. sp. cepae karena kemampuannya dalam memproduksi senyawa bioaktif. Penelitian ini bertujuan mengevaluasi dan menyeleksi isolat-isolat aktinomiset yang dapat menekan pertumbuhan F. oxysporum f. sp. cepae dan memacu pertumbuhan tanaman bawang merah. Penelitian dibagi menjadi empat tahap, yaitu: isolasi, seleksi, karakterisasi, dan identifikasi isolat aktinomiset yang potensial. Sebanyak 43 isolat berhasil diisolasi dari tanaman Liliaceae dan sebanyak 14 isolat berhasil diseleksi berdasarkan hasil uji keamanan hayati. Hasil uji antagonisme menunjukkan bahwa 14 isolat menghasilkan penghambatan terhadap F. oxysporum f. sp. cepae sebesar 3.67%–53.67%. Di antara 14 isolat tersebut, sebanyak 13 isolat mampu memproduksi enzim kitinase dengan indeks kitinolitik sebesar 0.31–1.38. Lima isolat terpilih yaitu: ABF42, ABF59, ACF45, AEF35, dan AEF45, mampu melarutkan fosfat dan memproduksi IAA dengan konsentrasi 24.82–82.88 ppm, namun hanya tiga isolat yang mampu memfiksasi nitrogen. Berdasarkan sikuen gen 16S rRNA, lima isolat tersebut berturut-turut teridentifikasi sebagai: Streptomyces rameus, S. lydicus, S. panaciradicis, S. seoulensis, dan S. fuscichromogenes.
Komposisi Formula Biobakterisida Berbahan Aktif Rizobakteri untuk Pengendalian Penyakit Busuk Lunak Pada Anggrek Phalaenopsis Hanudin Hanudin; Abdjad Asih Nawangsih; Budi Marwoto; Boedi Tjahjono
Jurnal Hortikultura Vol 23, No 3 (2013): September 2013
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v23n3.2013.p244-254

Abstract

Penyakit busuk lunak (PBL) yang disebabkan oleh Pectobacterium carotovorum subsp. carotovorum atau Pseudomonas viridiflava merupakan kendala utama dalam budidaya anggrek. Serangan patogen tersebut sangat merugikan petani, mengingat biaya investasi produksi anggrek tergolong tinggi. Oleh karena itu patogen tersebut harus dikendalikan menggunakan metode pengendalian yang ramah lingkungan, yaitu dengan mengaplikasikan biobakterisida berbahan aktif rizobakteri, seperti Bacillus subtilis dan Pseudomonas fluorescens. Tujuan penelitian ini ialah (1) mendapatkan komposisi bahan aktif dan bahan pembawa biobakterisida yang efektif mengendalikan penyakit busuk lunak pada anggrek Phalaenopsis, (2) mengetahui perubahan reaksi kimia formula biobakterisida dan pertumbuhan populasi bahan aktif (rizobakteri B. subtilis dan P. fluorescens) pada kondisi sebelum dan setelah difermentasikan, dan (3) mengetahui kompatibilitas antara B. subtilis, P. fluorescens, dan bahan pembawa biobakterisida. Percobaan dilaksanakan mulai Bulan Mei sampai dengan Desember 2009 di Laboratorium Bakteriologi Departemen Proteksi Tanaman, Institut Pertanian Bogor, dan Laboratorium Bakteriologi serta Rumah Kaca, Balai Penelitian Tanaman Hias, di Segunung, Cianjur, Jawa Barat. Ruang lingkup penelitian meliputi pembuatan propagul rizobakteri sebagai bahan aktif biobakterisida, pembuatan formula biobakterisida, uji viabilitas bahan aktif, dan uji kemangkusan biobakterisida pada tanaman anggrek di rumah kaca. Hasil penelitian menunjukkan bahwa (1) perlakuan gabungan antara B. subtilis B12 dan P. fluorescens Pf10 yang difermentasikan dalam media ekstrak kotoran cacing (kascing) dan molase, merupakan perlakuan yang konsisten dapat menekan PBL pada anggrek Phalaenopsis dengan persentase penekanan sebesar 80%, (2) reaksi kimia formula biopestisida pada kondisi sebelum dan setelah fermentasi diindikasikan dengan perubahan pH basal medium yang sebelum fermentasi menunjukkan pH 3,75 dan berubah menjadi pH 3,50 setelah difermentasikan. Pertumbuhan populasi mikrob antagonis setelah fermentasi meningkat secara signifikan bila dibandingkan pada kondisi sebelum difermentasikan, dan (3) isolat bahan aktif (B. subtilis dan P. fluorescens) bersifat kompatibel dengan bahan pembawanya (ekstrak kascing dan molase).
Isolation of actinomycetes from maize rhizosphere from Kupang, East Nusa Tenggara Province, and evaluation of their antibacterial, antifungal, and extracellular enzyme activity Umi Fatmawati; Yulin Lestari; Anja Meryandini; Abdjad Asih Nawangsih; Aris Tri Wahyudi
Indonesian Journal of Biotechnology Vol 23, No 1 (2018)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2623.079 KB) | DOI: 10.22146/ijbiotech.33064

Abstract

Actinomycetes are the one of the components of the rhizospheric microbial population and useful for producing secondary metabolites such as lytic enzymes, antibiotics, and antifungal. The aim of the study was to isolate the actinomycetes from maize rhizosphere collected from Kupang, East Nusa Tenggara. The screening was focused on the actinomycetes that showed the ability to produce antibacterial, antifungal, and extracellular enzymes such as amylase, cellulase, and protease. The actinomycetes were isolated using Humic-Acid Vitamin B (HV) agar media. The antagonistic assay was tested against Escherichia coli, Staphylococcus aureus, Sclerotium rolfsii and Fusarium oxysporum. Isolate JKP-8 was an isolate that showed the highest activity in inhibiting the growth of E. coli and S. aureus bacteria. Isolate JKP-5 showed the highest activity in inhibiting the growth of F.oxysporum. There were no actinomycetes isolates that showed an ability to inhibit the growth of S. rolfsii fungus based on dual culture assay. JKP-3 and JKP-4 isolates exhibited the highest ability to hydrolyze amylum, while JKP-5 and JKP-8 isolates exhibited the highest ability to hydrolyze CMC. The results of the amplification of 16S rRNA gene in selected potential isolates JKP 5 and JKP 8 indicated that both isolates belong to the genus Streptomyces.
Screening of Rhizobacteria for Plant Growth Promotion and Their Tolerance to Drought Stress RAHAYU FITRIANI WANGSA PUTRIE; ARIS TRI WAHYUDI; ABDJAD ASIH NAWANGSIH; EDI HUSEN
Microbiology Indonesia Vol. 7 No. 3 (2013): September 2013
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.581 KB) | DOI: 10.5454/mi.7.3.2

Abstract

Rhizobacteria have been known for their capability as plant growth promoter through some mechanisms, directly and indirectly. The purpose of this research to screen rhizobacteria of Bacillus spp. and Pseudomonas spp. for their drought tolerance as plant growth promoter of maize (Zea mays). Screening of rhizobacteria as growth promoter of 47 isolates of Bacillus CR and 34 isolates of  Pseudomonas CRB resulted 24 and 9 isolates were able to stimulate the growth of maize sprouts, respectively. Further screening of those growth promoter of the rhizobacterial isolates to drought tolerance resulted 7 isolates of Bacillus CR and 6 isolates of Pseudomonas CRB that were able to grow on medium with osmotic pressure -1 and -2 MPa, respectively. Potential rhizobacterial isolates of growth promoter and drought tolerance were tested for antagonist mechanisms which aims to determine ability to live together in one carrier medium if to be made formulation. Both non antagonist rhizobacterial isolates were evaluated for their potential in producing exopolysaccharide (EPS) revealing that CRB 19 and CR 90 exhibited the highest activity of EPS production up to 0.346 mg mL-1 on medium with -2.0 MPa and 0.107 mg mL-1 on medium with -0.73 MPa, respectively. Based on 16S rRNA sequence analysis, it revealed CRB 19 and CR 90 had highest similarities to Pseudomonas aeruginosa strain B2 and Brevibacillus brevis B33, respectively. Those growth promoter and drought tolerant of Bacillus CR and Pseudomonas CRB had potency to be developed as inoculants in dry land agriculture.
SELECTION AND CHARACTERIZATION OF SIDEROPHORE-PRODUCING RHIZOBACTERIA AND POTENTIAL ANTAGONISTIC ACTIVITY TOWARD Ralstonia solanacearum Abdjad Asih Nawangsih; Ida Parida; Suryo Wiyono; Juang Gema Kartika
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 24 No. 2 (2017)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1186.787 KB) | DOI: 10.11598/btb.2017.24.2.406

Abstract

Ralstonia solanacearum is an important disease of tomato.  An alternative method to control the disease is the application of biocontrol agents.  Plant Growth-Promoting Rhizobacteria (PGPR) could be used as potential biocontrol agents.  PGPR with siderophores is among compounds having important role in disease suppression.  This experiment was conducted to select and characterize the siderophore-producing rhizobacteria from tomato and to determine their potential as antagonistic agents for R. solanacearum.  Candidates of the PGPR were isolated from tomato grown in West Java Province, Indonesia. The isolates were detected as siderophore-producing bacteria using CAS medium. Among 29 isolates producing siderophore and having negative result on hypersensitivity reaction, two isolates provided the widest diameter of inhibition zone toward R. solanacearum.  Both isolates were CP1C and CP2D with diameter of inhibition zone up to 3.6 and 7.0 mm, respectively.  Based on the sequence of 16S rDNA, isolate CP1C was identified as Brevundimonas sp., while isolate CP2D was identified as Enterobacter sp.  Both bacteria did not cause negative effect on the increasing plant height and dry weight of the plants, compared with control.
KELIMPAHAN BAKTERI RIZOSFER PADA SISTEM PHT-BIOINTENSIF SERTA KEMAMPUAN ANTAGONISMENYA TERHADAP Sclerotium rolfsii PADA KEDELAI Abdjad Asih Nawangsih; Tita Widjayanti .; Yana Anisa .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 2 (2014): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (688.214 KB) | DOI: 10.23960/j.hptt.214110-120

Abstract

Abundance of rhizospheric bacteria on the IPM-Biointensive system and their antagonistic activities toward Sclerotium rolfsii on soybean. Abundance of beneficial microorganisms in the soil is one of the active soil indicators the success of integrated pests management (IPM) system. Some beneficial groups of microorganisms can be used as biocontrol agents. This experiment was conducted to evaluate the effects of IPM-Biointensive by integrated application of resistant varieties, rice-straw mulch, and biocontrol agents on the abundance of rizospheric bacteria of soybean, also to evaluate the suppressiveness of the bacteria to the mycelial growth of S. rolfsii in vitro. Abundance of the bacteria was determined by isolation using serial dilution and plate-count techniques. Suppression to the fungus was evaluated using dual culture technique. Heat tolerant bacteria had the highest abundance (ranged 1011-1012 cfu/g soil), followed by non-fluorescence bacteria (1011 cfu/g soil), chitinolytic bacteria (106-109 cfu/g soil), and fluorescence bacteria with population range was 103-108 cfu/g soil. Gepak kuning variety grown with application of rice-straw mulch and PGPR (V2M1P1) caused the highest abundance of rizosphere bacteria. One of the heat tolerant bacteria, i.e. TP32, caused the highest suppression to the mycelial growth of S. rolfsii in vitro. Based on the morphology, physiology, and biochemical properties, the isolate was identified as Bacillus sp.
Isolasi dan Seleksi Bakteri Endofit untuk Pengendalian Penyakit Darah pada Tanaman Pisang Husda Marwan; Meity S. Sinaga; . Giyanto; Abdjad Asih Nawangsih
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 11 No. 2 (2011): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (70.101 KB) | DOI: 10.23960/j.hptt.211113-121

Abstract

Blood disease is one of the important diseases of banana in Indonesia. Endophytic bacteria have potencies as candidates of biocontrol agents to blood disease, because the bacteria colonized the same ecological niche with the plant pathogens. This research was conducted to isolate endophytic bacteria from banana root, and study their disease suppression ability to blood disease on banana. Ninety isolates of endophytic bacteria have been isolated from the root of banana. Average population densities of bacteria varied between 6,0 x 103 and 4,2 x 105 cfu/g fresh weight of root. Twenty seven isolates positively produced inhibition zone toward blood disease bacterium. Based on plant growth and disease suppression test, ten isolates promoted the growth of banana plant and four isolates suppressed the incidence of blood disease with ranged from 66,67 to 83,33%.
Utilization of Liquid Smoke to Suppress Blood Diseases on Bananas and Its Effects on the Plant Growth Imas Aisyah; Meity Suradji Sinaga; Abdjad Asih Nawangsih; Giyanto Giyanto; Gustan Pari
AGRIVITA, Journal of Agricultural Science Vol 40, No 3 (2018): OCTOBER
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i3.1390

Abstract

Liquid smoke is reported to be effective to inhibit some bacteria. The study aimed to evaluate the effects of liquid smoke (LS) from coconut shell (CS-LS), pinecone (P-LS), and oil palm branch (OPB-LS) on the incidences of blood disease, induced resistance to Ralstonia syzygii subsp. celebesensis, and plant growth. Two days after banana seedlings were treated with LS, ethylene, auxin, lignin, activities of the enzymes of peroxidase (POD) and phenylalanine ammonia lyase (PAL) on the root of the banana seedlings were measured. Then, the seedlings were inoculated by R. syzygii subsp. celebesensis. The plant growth and incidence of blood diseases were observed daily and the 31st day after the application of LS and this bacteria inoculation. The results showed that the CS-LS, P-LS, and OPB-LS at all tested concentration could suppress the incidence of blood diseases up to 100 %, induce resistance of banana seedlings to the R. syzygii subsp. celebesensis with increased levels of ethylene, auxin, lignin, activities of POD and PAL, and plant growth, significantly. Based on the effectiveness of this bacterial control and the ability to promote the growth of banana seedlings test, it is recommended that the most effective treatment is P-LS 0.5 %.
Karakterisasi Bakteri Penyebab Busuk Lunak pada Umbi Porang (Amorphophallus muelleri) Menggunakan Primer PCR Spesifik Ely Lailatul Maghfiroh; Abdul Munif; Abdjad Asih Nawangsih; Alina Akhdiya
Jurnal Ilmu Pertanian Indonesia Vol. 27 No. 3 (2022): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.27.3.463

Abstract

Porang is one of the export commodities whose production is currently intensive by the Indonesian Government. Soft rot disease is one of the obstacles in cultivation and post-harvest of porang, which results in a decrease in productivity and quality of porang. This study aims to isolate pathogenic bacteria that infect porang tubers and determine their morphological and molecular characters. A total of 43 bacteria were isolated from porang tubers with soft rot symptoms from Malang Regency. Ten isolates of them showed positive reactions in the hypersensitivity test. Amylolytic, pectinolytic, mannanase, and proteolytic activities were shown by three bacterial isolates, including B4, B7, and BLUB15, by forming a clear zone around the inoculation point. The results of the decay test showed that B4 isolate had the most severe signs of decay, up to 97.88% in potato tubers and 37.12% in the porang frog. A pair of ExpccR/ExpccF PCR primers successfully detected B4 isolate as Pectobacterium carotovorum subsp. carotovorum (Pcc) in the 550 bp band. Unexpectedly, identifying the other two isolates did not show any similarity to the bacteria that cause soft rot, which was previously known. The two isolates showed similarities to Empedobacter sp. B7 (98.7%) and Pseudomonas sp. BLUB15 (97.6%) as a result of 16S rRNA gene analysis. So far, based on the existing literature, this is the first report of phytopathogenic bacteria causing soft rot outside the genus Pectobacterium and Dickeya. Keywords: decay, glucomannan, mannanase enzyme, pectobacterium
Potential of the Endophytic Bacteria from Lantana camara, Palm Oil, and Mangroves to control Meloidogyne spp. on Eggplant Siti Zulaiha; Abdul Munif; Abdjad Asih Nawangsih
Jurnal Fitopatologi Indonesia Vol. 18 No. 5 (2022): September 2022
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.18.5.213-221

Abstract

Nematoda puru akar (NPA) Meloidogyne spp. merupakan salah satu patogen penting pada tanaman terung. Puru akar sangat merugikan karena menurunkan hasil produksi. Penelitian ini bertujuan mendapatkan bakteri endofit dari Lantana camara dan mengevaluasinya bersama bakteri endofit dari akar kelapa sawit, dan mangrove yang berpotensi untuk mengendalikan Meloidogyne spp. dan menentukan pengaruhnya pada pertumbuhan tanaman terung, serta mengidentifikasi bakteri endofit yang potensial sebagai agens pengendalian hayati. Potensi agens hayati mengacu pada hasil pengujian uji mortalitas, uji senyawa organik volatil (SOV) dan uji penekanan puru akar dievaluasi dengan mengukur persentase penurunan jumlah puru, kerusakan akar, dan jumlah paket telur. Hasil uji menunjukkan seluruh isolat bakteri endofit berpotensi menyebabkan mortalitas, menghasilkan SOV, dan dapat menekan persentase jumlah puru pada akar, serta memberikan pengaruh terhadap bobot dan tinggi tanaman. Bakteri endofit LCA5 dan LCA13 menyebabkan mortalitas lebih dari 90% terhadap Meloidogyne spp. J2. Selama 24 jam dan pada uji SOV menyebabkan mortalitas lebih dari 60% selama 24 jam. Bakteri endofit yang memiliki potensi sebagai pengendalian hayati diidentifikasi sebagai Dyella marensis, Stenotrophomonas rhizophilla, dan Providencia vermicola.
Co-Authors . Giyanto . Giyanto ABDUL MUNIF Abdul Munif ABDUL MUNIF Abidin, Anugrawati Af'idzatuttama Af’idzatuttama Aisyah Imas Alina Akhdiya Anak Agung Keswari Krisnandika Anja Meryandini Aris Tri Wahyudi Asysyuura, Asysyuura Boedi Tjahjono Budi Marwoto DIMAS ANDRIANTO Edelwinna, Tiara EDI HUSEN Efi Toding Tondok Eka Wijayanti Eka Wijayanti Ely Lailatul Maghfiroh Eny Widajati Erina Sulistiani Firdaus, Muhamad Fajar Fitri Fatma Wardani Giyanto Gustan Pari Gustan Pari Haerani Haerani Hanudin Hanudin Husda Marwan Ida Parida IKA DAMAYANTI Iman Rusmana Imas Aisyah isnainy dinul Mursyalatiyus Juang Gema Kartika Kikin H Mutaqin Krisnandika, A. A. Keswari Marianah, Lisa Meity S. Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meliah, Siti Muhammad Evan Nurrahmawan Purwanto, Ukhradiya Magharaniq Safira RAHAYU FITRIANI WANGSA PUTRIE Sigiro, Ria Heni Siti Zulaiha Sudir Sudir, Sudir Sulistiyani Suryo Wiyono Tita Widjayanti Tita Widjayanti . Toding Tondok, Efi TRI ASMIRA DAMAYANTI Umi Fatmawati Wati, Cheppy Wiraswati, Sri Martina Yana Anisa . YULIN LESTARI Zhenita Vinda Tri Handini