Article Per Year (5 Year)
p-Index From 2021 - 2026
4.232
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biogenesis: Jurnal Ilmiah Biologi Jurnal Hortikultura AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Entomologi Indonesia Makara Journal of Science Biodiversitas Journal of Biological Diversity
Abdjad Asih Nawangsih
Department Of Plant Protection, Faculty Of Agriculture, IPB University, Jl. Meranti, Kampus IPB Dramaga, Bogor, West Java, Indonesia 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

Published : 45 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 45 Documents
Search

 1   2   3   4   5 
Pengaruh Bakteri Endofit Padi terhadap Penyakit Busuk Bulir Bakteri dan Pertumbuhan Tanaman Padi: Effects of Rice Endophytic Bacteria on Bacterial Grain Rot Disease and Rice Growth Rahmadhani, Nahrisa; Munif, Abdul; Nawangsih, Abdjad Asih
Jurnal Fitopatologi Indonesia Vol. 21 No. 1 (2025): Maret 2025
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.21.1.51-63

Abstract

Effects of Rice Endophytic Bacteria on Bacterial Grain Rot Disease and Rice Growth Bacterial grain rot disease, caused by Burkholderia glumae, is an important disease that can reduce rice yields and is a seed-borne disease. Biological control through biopriming using endophytic bacteria is an alternative disease control method that has been widely used in various crops. This study aimed to assess the potential of endophytic bacteria from six rice varieties using biopriming techniques in controlling B. glumae and their ability to stimulate rice growth. The research stages consisted of isolation and biosafety testing of endophytic bacteria with potential as biological agents, in vitro antagonism assay against B. glumae using the cross streak method, phytotoxicity assay of endophytic bacteria on rice seeds, selection of potential bacteria using the analytical hyphenation process (AHP) method, and examining the ability of biopriming to control B. glumae and its effect on rice vegetative growth. A total of 133 endophytic bacterial isolates were successfully isolated from six rice varieties, and 27 isolates met the biosafety test requirements. In vitro and in vivo assay showed that eight isolates exhibited antagonistic activity against B. glumae, and 25 isolates increased rice seed germination. Three isolates were selected based on AHP results, i.e. BBZN3, BBAR4, and AKPAT4. Of these three isolates, AKPAT4 was the best at increasing the vigor index and germination level of rice seeds, as well as suppressing disease incidence and severity.
Biopriming Potential Screening of Endophytic Bacteria to Prevent Amorphophallus muelleri Blume Agaist Soft Rot Disease Through In-vitro and In-Planta Screening Ely Maghfiroh; Ika Roostika; Deden Sukmadjaja; Abdul Munif; Abdjad Asih Nawangsih; Alina Akhdiya
HAYATI Journal of Biosciences Vol. 33 No. 4 (2026): July 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.4.1147-1156

Abstract

Soft rot is the serious problem in cultivation and post harvest of  A. muelleri Blume Yam (Elephant foot Yam).  This experiment aims is to screen and characterize endophytic bacteria that potentially prevent soft rot on porang (A. muelleri Blume) through in-vitro and in-planta screening. Screening of the bacteria resulted three isolates i.e. EAP10, EAP17, and Bacillus velezensis EG113 could inhibit soft rot caused by P. carotovorum B4. The three isolates demonstrated that EAP10, EAP17, Bv EG113 have anti quorum sensing (AQS) activity against Chromobacter violaceum and have capability to decrease the soft rot incidence of porang plantlets artificially infected with Pectobacterium carotovorum B4. Compared to the other selected endophytes, B. velezensis EG113 showed the best capability to decrease the disease incidence (75%). Physiological characterization of the selected endophytic isolates showed cellulolytic, proteolytic, and IAA production capability. The 16S rRNA sequences homology analysis of endophytic isolates showed similarity to Acinetobacter radioresistance (100%) and Bacillus aryabhattai (99%) or Priestia aryabhattai (revision name) respectively for EAP10 and EAP17 isolates. The results suggested that B. velezensis EG113 is potentially to be develop as biopriming agent for early protection of porang.
SELECTION AND CHARACTERIZATION OF SIDEROPHORE-PRODUCING RHIZOBACTERIA AND POTENTIAL ANTAGONISTIC ACTIVITY TOWARD Ralstonia solanacearum Abdjad Asih Nawangsih; Ida Parida; Suryo Wiyono; Juang Gema Kartika
BIOTROPIA Vol. 24 No. 2 (2017): BIOTROPIA Vol. 24 No. 2 August 2017
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1186.787 KB) | DOI: 10.11598/btb.2017.24.2.406

Abstract

Ralstonia solanacearum is an important pathogen of tomato. An alternative method to control this disease is through the application of biocontrol agents. Plant Growth-Promoting Rhizobacteria (PGPR) can be used as potential biocontrol agents, and PGPR producing siderophores play an important role in disease suppression. This experiment was conducted to select and characterize siderophore-producing rhizobacteria from tomato and to determine their potential as antagonistic agents against R. solanacearum. Candidates of PGPR were isolated from tomato plants grown in West Java Province, Indonesia. The isolates were screened for siderophore production using CAS medium. Among the 29 siderophore-producing isolates that showed a negative hypersensitivity reaction, two isolates exhibited the widest inhibition zones against R. solanacearum. These isolates were CP1C and CP2D, with inhibition zone diameters of up to 3.6 mm and 7.0 mm, respectively. Based on 16S rDNA sequencing, isolate CP1C was identified as Brevundimonas sp., while isolate CP2D was identified as Enterobacter sp. Both isolates did not negatively affect plant height or plant dry weight when compared with the control.
TRFLP analysis for revealing the diversity of rice phyllosphere bacteria SRI MARTINA WIRASWATI; ARIS TRI WAHYUDI; IMAN RUSMANA; ABDJAD ASIH NAWANGSIH
Biodiversitas Journal of Biological Diversity Vol. 19 No. 5 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190521

Abstract

Wiraswati SM, Wahyudi AT, Rusmana I, Nawangsih AA. 2018. TRFLP analysis for revealing the diversity of rice phyllosphere bacteria. Biodiversitas 19: 1743-1749. Phyllosphere environment of rice plant is usually inhabited by diverse bacteria which mostly contribute beneficial effects to the plant fitness. TRFLP method is a rapid and straightforward method to determine the bacterial diversity of many environments, including rice phyllosphere environment. This study aimed to analyze rice phyllosphere bacterial diversity of healthy rice plant cultivar Ciherang obtained from Sukabumi, Jasinga, and Situgede. The bacterial genomes were amplified and digested with two restriction enzymes, i.e., MspI and BstUI. The bacterial diversity (H’ index) and evenness (E index) were calculated from the peak value. From TRFs analysis, Betaproteobacteria and Pseudomonadales were dominantly found in nearly all samples with different relative abundance. In addition, Alphaproteobacteria and Gammaproteobacteria were also dominant in the several samples. The unique bacteria groups were inhabited in the sample from specific regions with certain growth phase. This finding informs us that the geographical factors might be more influent than the growth phase factor. Furthermore, the bacterial diversity and evenness of the metagenomic approach are higher than cultivation-dependent approach.
Streptomyces spp. from rhizosphere soil of maize with potential as plant growth promoter Aris Tri Wahyudi; J.A. Priyanto; HANIFA NUR Fijrina; HIMA DEWI Mariastuti; ABDJAD ASIH Nawangsih
Biodiversitas Journal of Biological Diversity Vol. 20 No. 9 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200916

Abstract

Abstract. Wahyudi AT, Priyanto JA, Fijrina HN, Mariastuti HD, Nawangsih AA. 2019. Streptomyces spp. from rhizosphere soil of maize with potential as plant growth promoter. Biodiversitas 20: 2547-2553. Actinomycete is one group of rhizobacteria that plays an important role as a plant growth promoter. This study was aimed to evaluate the potential of Actinomycetes isolated from maize rhizosphere in promoting plant growth in vitro including their ability to produce IAA, promote maize sprout growth, solubilize phosphate, and grow in N-free medium. Thirty isolates have been isolated from maize rhizosphere using a spread plate method. All 30 isolates were probably not pathogenic to plants as tested by hypersensitivity reaction test on tobacco leaves. Based on the colorimetric assay, 30 isolates (100%) were able to produce IAA with concentrations ranging from 1.05 to 26.89 ppm. The highest concentration of IAA (26.89 ppm) produced by ARJ 21 and the lowest IAA concentration (1.05 ppm) produced by ARJ 12. By using the Ragdoll method, it showed that 9 isolates (30%) were able to promote maize sprout growth significantly on five growth parameters including primary root length, stem length, number of lateral roots, wet weight and dry weight. Twenty-one isolates (70%) were capable of solubilizing phosphate in Pikovskaya medium containing tricalcium phosphate. Also, 30 isolates (100%) were able to grow on N-free medium, suggesting their ability to fix nitrogen. Based on 16S-rRNA, five potential isolates with plant growth-promoting properties were highly similar to Streptomyces spp. Based on their potential characters, these Actinomycetes isolates have the potential to be further developed as a biofertilizer agent for sustainable maize farming.
Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth UMI FATMAWATI; ANJA MERYANDINI; ABDJAD ASIH NAWANGSIH; Aris Tri Wahyudi
Biodiversitas Journal of Biological Diversity Vol. 20 No. 10 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d201027

Abstract

Abstract. Fatmawati U, Meryandini A, Nawangsih AA, Wahyudi AT. 2019. Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth. Biodiversitas 20: 2970-2977. Actinomycetes which colonized plant rhizosphere has a vital role in improving plant growth by producing Indole-3-Acetic Acid (IAA). The aim of this study was to evaluate the potency of actinomycetes isolated from soybean rhizosphere as promoter agents for plant growth in vitro. Fifty actinomycetes isolates were successfully isolated from soybean rhizosphere. Based on the colorimetric methods, 35 isolates can produce IAA in various concentration, in the range of 0.46-30.6 mg/L. Seed germination assay using Ragdoll methods revealed that 26 isolates significantly promoted germination parameters, including the hypocotyl and the radicular length, the number of the lateral roots, and dry weight of the plant. Also, 14 from 26 isolates showed phosphate solubilizing activity in different phosphate-solubilizing index ranging from 1.25-2.62. Eight isolates were able to grow in N-free medium, indicating that these isolates have the ability in fixing nitrogen. Out of 23 from 26 isolates were detected to produce siderophore. All the tested isolates show chitinase production except ASR 55. Based on the observed parameters, it showed that there are four potential isolates (ASR 46, ASR 58, ASR 75 and ASR 76) as promising plant-growth promoters, phosphate solubilizer, nitrogen fixer, siderophore and chitinase producer. Based on the result of 16S rRNA sequence analysis, four potential isolates were identified as Streptomyces spp. in different taxa of strains and species.
Rice phyllosphere bacteria producing antifungal compounds as biological control agents of blast disease SRI MARTINA WIRASWATI; ABDJAD ASIH NAWANGSIH; IMAN RUSMANA; Aris Tri Wahyudi
Biodiversitas Journal of Biological Diversity Vol. 21 No. 4 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210401

Abstract

Abstract. Wiraswati SM, Nawangsih AA, Rusmana I, Wahyudi AT. 2020. Rice phyllosphere bacteria producing antifungal compounds as biological control agents of blast disease. Biodiversitas 21: 1273-1278. In vitro analysis of rice phyllosphere bacteria can be developed as biocontrol agents of blast disease using their antifungal activity. To prove the efficacy of bacterial isolates in reducing the severity of blast disease, a greenhouse experiment was conducted. Furthermore, antifungal compounds produced by potential bacterial isolate were also identified using Thin Layer Chromatography (TLC) and Liquid Chromatography-Mass Spectrometry/ Mass Spectrometry (LC-MS/MS) analysis. The application of seven rice phyllosphere bacterial isolates significantly reduced the severity of blast disease in rice (var: Ciherang). Surprisingly, the isolate STGG 14 is the most effective with 70.83% of blast disease reduction. Isolate STGG 14 has been identified as Bacillus subtilis subsp. subtilis which is known as various bioactive compounds producer. It was confirmed through identification of antifungal compounds produced by isolate STGG 14. The bioautography test shows that crude bioactive compounds from isolate STGG 14 could inhibit P. oryzae race 173. On the further analysis using LC-MS/MS, four bioactive compounds isolated from isolate STGG 14 were identified as moracin C, psoralen, 4,6-dimethyl-3 (4’-hydroxyphenyl) coumarin and xanthotoxin. All identified compounds except moracin C, belong to the coumarin group. According to the previous studies, among all compounds, psoralen and xanthotoxin displayed antifungal activity against several fungal pathogens.
Rhizosphere Streptomyces formulas as the biological control agent of phytopathogenic fungi Fusarium oxysporum and plant growth promoter of soybean MAYA SARI; ABDJAD ASIH NAWANGSIH; Aris Tri Wahyudi
Biodiversitas Journal of Biological Diversity Vol. 22 No. 6 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220602

Abstract

Abstract. Sari M, Nawangsih AA, Wahyudi AT. 2021. Rhizosphere Streptomyces formulas as the biological control agent of phytopathogenic fungi Fusarium oxysporum and plant growth promoter of soybean. Biodiversitas 22: 3015-3023. Rhizosphere Streptomyces are considered as promising sources of plant growth-promoting rhizobacteria (PGPR) and biocontrol agents against pathogenic fungi, particularly Fusarium oxysporum causing root rot, cotyledon rot, hypocotyl rot, and stunted growth in soybean. Formulation of rhizosphere Streptomyces with appropriate carrier materials is necessary to facilitate storage and application in plants. This study aimed to develop a formulation of rhizosphere Streptomyces, apply the formula to control F. oxysporum, and promote soybean plant growth. Five Streptomyces isolates, i.e., Streptomyces panaciradicis ARK 13, Streptomyces tritolerans ARK 17, Streptomyces recifensis ARK 63, Streptomyces tendae ARK 91, and Streptomyces manipurensis ARK 94 were used in this study. All of the isolates could grow in potato broth, rice bran extract, and molasses as alternative media. The highest biomass produced from the molasses growth medium. All five isolates had antifungal activity against F. oxysporum with the inhibition percentage ranging from 41% to 76%, and all of them were detected to have the iaaM gene. Indole-3-acetic acid (IAA) hormone produced by these isolates were ranging from 8.99-15.14 mg L-1, with the phosphate solubilization index of 2.13-2.47. Five rhizosphere Streptomyces formulas with the main carrier of peat could maintain the viability with the population density of 108 CFU g-1 for 8 weeks of storage at room temperature. Two formulas, F17 and F94, were the best formulas to control disease caused by F. oxysporum with disease suppression of 74% in sterile soil and 80-85% in non-sterile soil. Formula F17 and F94 significantly increased soybean growth in sterile and non-sterile soils. Therefore, these formulas could be recommended as biocontrol and plant growth promoters of soybean.
Screening of chitinase-producing rhizosphere actinomycetes and their genetic diversity Aris Tri Wahyudi; NAUFAL GHAZI FITHRIANSYAH; MUHAMMAD FAIZ AMRI; JEPRI AGUNG PRIYANTO; ABDJAD ASIH NAWANGSIH
Biodiversitas Journal of Biological Diversity Vol. 22 No. 10 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d221008

Abstract

Abstract. Wahyudi AT, Fitriansyah NG, Amri MF, Priyanto JA, Nawangsih AA. 2021. Screening of chitinase-producing rhizosphere actinomycetes and their genetic diversity. Biodiversitas 22: 4186-4192. Soil microbial community, especially rhizosphere actinomycetes, plays an important role in protecting plants from phytopathogenic fungi by producing various agroactive compounds, including mycolytic enzymes particularly chitinase. The objective of this study was to investigate chitinase activity and analyze the diversity of family 19 chitinase genes of actinomycetes isolated from maize and soybean rhizosphere. Of fivety actinimycetes, Seventeen isolates exhibited chitinolytic activities and formed a hydrolytic zone around the colony with chitinolytic index ranging from 0.49±0.01 to 2.15±0.69, qualitatively tested in 0.3% chitin agar medium. Six selected isolates (ARJ 36, ARJ 81, ARJ 15, ARK 17, ARK 143, ARK 103) showed chitinase activities ranging from 0.157±0.04 to 0.440±0.09 U/mL, based on chitinolytic index. The production of chitinase was confirmed by the presence of family 19 chitinase encoding genes from Streptomyces sp. Some conserved regions and essential amino acid residues were also detected. This study suggests that chitinolytic actinomycetes isolated from maize and soybean rhizosphere can be studied further as biological control candidates for controlling phytopathogenic fungi.
Diversity of leaf rust resistance in Indonesian sorghum genetic resources FAWWAZ DINANTY; DESTA WIRNAS; TRIKOESOEMANINGTYAS TRIKOESOEMANINGTYAS; ABDJAD ASIH NAWANGSIH
Biodiversitas Journal of Biological Diversity Vol. 23 No. 5 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230538

Abstract

Abstract. Dinanty F, Wirnas D, Trikoesoemaningtyas, Nawangsih AA. 2022. Diversity of leaf rust resistance in Indonesian sorghum genetic resources. Biodiversitas 23: 2570-2579. Sorghum is an alternative source as a substitute for rice. Leaf rust (Puccinia purpurea) is a constraint in sorghum cultivation and resistance to rust disease is a condition for releasing sorghum varieties. This study aimed to screen sorghum accessions for resistance to leaf rust disease. The screening was carried out on 48 sorghum accessions and seven check varieties, namely Numbu, Kawali, Bioguma 1, Pahat, Soraya 1, Soraya 2, and Soraya 3. Evaluation of leaf rust resistance under field conditions was carried out in IPB University experimental field Cikarawang-Bogor (275 asl) from February 2021 to June 2021. The experiment was conducted in an augmented design with four replications for check varieties. Identification of leaf rust resistance was also carried out using molecular markers designed from the Lr34 and Rp1D genes. The results revealed that 5 genotypes, namely genotypes 31 (Demak 2), 24 (IS 18551), 43 (Lokal bima 3), 17 (ICSV 93036), and 33 (Demak 5) were resistant to leaf rust with high yield potential. The results of identification using molecular markers showed that all accessions had Lr34 and Rp1D gene fragments.
Co-Authors , Giyanto . Giyanto Abdul Munif ABDUL MUNIF ABDUL MUNIF Abidin, Anugrawati Af'idzatuttama Af’idzatuttama Aisyah Imas Alina Akhdiya Alina Akhdiya Anak Agung Keswari Krisnandika Anja Meryandini Aris Tri Wahyudi Asysyuura, Asysyuura Boedi Tjahjono Budi Marwoto CHEPPY WATI Deden Sukmadjaja DEMMA ZILBA BUDIMAN Desta Wirnas DIMAS ANDRIANTO EDI HUSEN Efi Toding Tondok Eka Wijayanti Eka Wijayanti Ely Lailatul Maghfiroh Ely Maghfiroh Eny Widajati Erina Sulistiani FARHANTYO FAUZAN DWINUGROHO FAWWAZ DINANTY Firdaus, Muhamad Fajar Fitri Fatma Wardani Giyanto Giyanto Giyanto GIYANTO GIYANTO GIYANTO GIYANTO Gustan Pari Gustan Pari Haerani Haerani HANIFA NUR Fijrina Hanudin Hanudin HIMA DEWI Mariastuti Husda Marwan Ida Parida IKA DAMAYANTI Ika Roostika Iman Rusmana Imas Aisyah isnainy dinul Mursyalatiyus Ivan Permana Putra J.A. Priyanto Jepri Agung Priyanto Juang Gema Kartika Kikin H Mutaqin Krisnandika, A. A. Keswari LISA MARIANAH Marianah, Lisa MAYA SARI Meity S. Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meliah, Siti Muhammad Evan Nurrahmawan MUHAMMAD FAIZ AMRI Nahrisa Rahmadhani NAUFAL GHAZI FITHRIANSYAH Purwanto, Ukhradiya Magharaniq Safira RAHAYU FITRIANI WANGSA PUTRIE Sigiro, Ria Heni Siti Meliah Siti Zulaiha SRI MARTINA WIRASWATI SRI MARTINA WIRASWATI Sudir Sudir, Sudir Sulistiyani SURYO WIYONO Suryo Wiyono Suryo Wiyono Tiara Edelwinna Tita Widjayanti Tita Widjayanti . TRI ASMIRA DAMAYANTI Trikoesoemaningtyas UMI FATMAWATI Umi Fatmawati Wati, Cheppy WIDYA ESTI PURWANINGTYAS Wiraswati, Sri Martina Yana Anisa . YULIN LESTARI Zhenita Vinda Tri Handini