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Epibion Makrofit Pantai Berpasir di Kabupaten Jepara, Jawa Tengah (Epibiont Macrophyte on Sandy Beach, in the Regency of Jepara, Central Java) Irwani Irwani; Norma Afiati
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 18, No 1 (2013): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (279.175 KB) | DOI: 10.14710/ik.ijms.18.1.30-38

Abstract

Epibion merupakan organisme yang hidup melekat pada berbagai jenis substrat alamiah di dasar perairan dan berperan penting sebagai pemakan sisa. Bandengan dan Pulau Panjang adalah dua lokasi pantai berpasir yang terletak berdekatan di Kabupaten Jepara Jawa Tengah. Studi ini menginvestigasi epibion pada makrofit, yaitu makroalgae dan lamun di kedua lokasi, dengan metode survei. Epibion utama kedua jenis makrofit tersebut adalah mikroalgae dan mikrozoobentos, dengan jumlah dan keanekaragaman yang relatif tidak berbeda. Secara lebih detil, epibion utama makroalgae umumnya adalah mikroalgae planktonik dan mikrozoobentos, sedangkan epibion utama lamun adalah mikroalgae planktonik yang bersifat epifit. Struktur komunitas bentik yang mengandung Polychaeta dalam jumlah jenis dan jumlah individu terbanyak dibandingkan Crustacea dan Mollusca, dan tidak dijumpainya genera lamun pionir Halophila dan Halodule di kedua lokasi, mengindikasikan masih cukup baiknya kualitas hayati kedua pantai wisata tersebut. Kata kunci: epibion, lamun, makroalga,  pantai berpasir, Jepara Epibiont ​​is known as living organisms attached to various natural benthic substrates and plays an important role as scavenger. Bandengan and Pulau Panjang are two sandy beaches close to each other located at Jepara, Central Java. This study investigates epibionts on macrophytes, which are macroalgae and seagrasses in both locations, by means of survey method. In general, major constituent of epibionts in those two macrophytes are microalgae and microzoobenthos, with relatively similar individual number and diversity. In more details, epibionts in macroalgae is planktonic microalgae and microzoobenthos, whereas, for seagrass it is of epiphytic microalgae. Benthic community structure which have more abundant and more diverse Polychaeta compared to that of Crustacea and Mollusca, combined with no existence of pioneer seagrass, i.e., Halophila and Halodule indicating that both beaches have still retained relatively good quality ecosystem for tourism. Keywords: epibiont, seagrass, macroalgae, sandy beach, Jepara
Characterization of Some Commercially Important Octopus (Mollusca: Cephalopoda) from Indonesian Waters using Mitochondrial DNA Cytochrome Oxidase Sub-Unit I (Mt-DNA COI) Nenik Kholilah; Norma Afiati; Subagiyo Subagiyo
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 26, No 1 (2021): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.26.1.17-26

Abstract

As per the FAO data, octopus identification is very limited in the species level at world fishery and also they are cryptic nature. On the other hand, Indonesia is one of the top ten highest octopus exporters. This study therefore aimed to determine the species of octopus based on phylogenetic analysis of mt-DNA COI. Octopuses were collected from nine different locations throughout Indonesia, i.e., Anambas, Bangka-Belitung, Cirebon, Karimunjawa, Tuban, Lombok, Buton, Wakatobi and Jayapura. Samples were mostly in the form of tentacles that were directly collected from fishermen. After being preserved in 96% ethanol, the sample was extracted in 10% chelexÒ, PCR amplification using Folmer’s primer then was further analysed by sequencing in Sanger methods. Of the 24 samples sequenced, the results recognized four species Octopodidae belongs to the three genera, named Amphioctopus aegina, Hapalochlaena fasciata, Octopus laqueus and Octopus cyanea. Mean pair-wise distances of within-species were ranged from 0 to 5.5 % and between-species was ranged from 12.9 to 15.8 %. This study distinctly confirmed the difference between genus Amphioctopus and Hapalochlaena (15.5 %), as also between O. laqueus and O. cyanea (12.9%) which was previously not completely distinguished. Although performing species identification using DNA sequences for shallow-water benthic octopus species is perhaps considered premature, this study indicated the possible application of COI sequences for species identification, thereby providing a preliminary dataset for future DNA barcoding of octopus, in particular for Indonesia waters.
PENGOLAHAN LIMBAH CAIR UREA MENGGUNAKAN PROSES GABUNGAN ACTIVATED MICROALGAE DAN NITRIFIKASI-DENITRIFIKASI AUTOTROFIK: UJI DENGAN RANCANGAN TAGUCHI Indro Sumantri; Sumarno Sumarno; Norma Afiati
Reaktor Volume 14, Nomor 1, April 2012
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (75.365 KB) | DOI: 10.14710/reaktor.14.1.79-85

Abstract

TREATMENT OF UREA WASTEWATER WITH COMBINED PROCESS OF ACTIVATED MICROALGAE AND NITRIFICATION-DENITRIFICATION AUTOTROPHIC: EVALUATION WITH TAGUCHI DESIGN. The conventional process for treatment wastewater from urea plant usually use of micro algae process or heterotrophic bacterial nitrification denitrification. Micro algae process use different type of micro algae. The advantage is cheap because used only little bit of P nutrient but cannot used for ammonium removal. Heterotrophic bacterial nitrification denitrification process needed high organic carbon input so that treatment cost so expensive. The objective of the research work was to investigate the potential of combination of special type micro algae process with an autotrophic nitrification-denitrification process. Micro algae species used in micro algae process have ability either for ammonium removal or withstand in high ammonium concentration. Autotrophic nitrification denitrification process used nitrifying bacterial/sludge as the biocatalyst. The origin of the nitrifying sludge was an activated sludge obtained from a particle board industry wastewater treatment plant where nitrification occurred in the aeration basin. Enrichment and breeding of the nitrifying sludge were conducted in high ammonium concentration and autotrophic condition. Based on experiment, enrichment and breeding micro algae which have ability either for ammonium removal or withstand in high ammonium concentration quite easy. By screening experiment with seven variable: MLSS, detention time, NH3-N concentration, aeration, CaCO3 concentration, micronutrient, N:P ratio, obtained the best level of variables are NH3-N concentration, aeration, CaCO3 concentration at high level. Evaluation limiting substrate inhibition of ammonium to nitrifying bacterial growth also unproved experimentally.Proses konvensional untuk mengolah limbah cair industri urea biasanya menggunakan proses alga mikro atau bakteri heterotropik nitrifikasi-denitrifikasi. Proses alga mikro dapat menggunakan berbagai jenis alga mikro. Keuntungannya adalah murah karena hanya memerlukan nutrien P sedikit tetapi tidak dapat digunakan untuk menyusutkan amoniak. Proses nitrifikasi-denitrifikasi bakteri heterotropik memerlukan asupan karbon yang tinggi sehingga pengolahan  menjadi mahal. Tujuan saat ini untuk penelitian adalah untuk mempelajari kombinasi yang potensial untuk proses alga mikro jenis tertentu dengan proses nitrifikasi-denitrifikasi ototrofik. Jenis alga mikro yang digunakan dalam proses alga mikro mempunyai kemampuan baik untuk penyusutan amoniak atau tahan dalam konsentrasi amoniak tinggi. Proses nitrifikasi-denitrifikasi ototrofik menggunakan bakteri nitrifikasi/lumpur sebagai biokatalis. Lumpur nitrifikasi awal adalah lumpur aktif kolam aerasi unit pengolahan limbah cair industri partikel board Pengayaan dan pembibitan lumpur nitrifikasi dilakukan dalam konsentrasi amoniak yang tinggi dan kondisi ototrofik. Berdasarkan penelitian, pengayaan dan pembibitan alga mikro yang mempunyai kemampuan untuk penyusutan amoniak dan tahan konsentrasi amoniak yang tinggi mudah. Evaluasi substrat pembatas penghambat amonium terhadap pertumbuhan alga tidak terbukti. Tujuh variabel yang dipilih lewat penapisan adalah : MLSS, waktu tinggal, konsentrasi NH3-N, laju aerasi, kadar CaCO3, nutrien mikro, rasio N:P. Variabel yang berpengaruhadalah konsentrasi NH3-N, laju aerasi, kadar CaCO3.Keywords: autotrophic nitrification-denitrification process; enrichment and breeding of the nitrifying sludge; micro algae proces
RESTOCKING SPECIES IKAN LOKAL DI PERAIRAN DANAU RAWA PENING (Restocking Local Fish Species in Rawa Pening) Max Rudolf Muskananfola; Norma Afiati; Abdul Ghofar
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 9, No 1 (2013): JURNAL SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.9.1.23-28

Abstract

Kompleksnya permasalahan yang terjadi di Rawa Pening telah berdampak pada menurunnya potensi sumberdaya perikanan khususnya populasi spesies ikan lokal. Kehilangan spesies lokal (spesies endemik) adalah kerugian besar, mulai dari hilangnya ‘warisan alam’ sehingga tidak dikenali lagi oleh anak cucu ataupun generasi yang akan datang, hilangnya nilai manfaat dari spesies tersebut, hingga gangguan keseimbangan ekologis akibat ada relung ekologi yang kosong. Tim Hibah Pengabdian Masyarakat FPIK Undip sebagai akademisi mempunyai tanggung jawab untuk berperan memberikan solusi atas permasalahan yang terjadi di Rawa pening, Salah satu solusi yang ditawarkan adalah Restocking ikan. Program ini dilaksanakan selama 5 bulan dengan 3 (tiga) tahapan. Pertama, Ecological Review yang terdiri dari program sosialisasi, pelatihan teknik restocking dan survai lingkungan serta lokasi untuk pertimbangan jenis biota yang dipilih untuk ditebar, ikan yang dipilih adalah ikan Nila Merah (Oreochromis niloticus). Kedua, Pre–Restocking, tahap ini adalah pengadaan/pembelian benih ikan untuk restocking serta aklimatisasi. Ketiga, Restocking, merupakan tahap inti kegiatan pengabdian masyarakat, jumlah ikan yang ditebar sebanyak 6,000 ekor bibit ikan dengan ukuran 5-10 cm, dan ditebar di tiga lokasi yang telah ditentukan pada saat survai, jadi masing–masing titik ditebarkan 2,000 ekor bibit ikan .Ketiga, Monitoring, kegiatan ini dilakukan untuk mengetahui keberhasilan program sekaligus evaluasi kekurangan–kekurangan pada pelaksanaan program yang dapat digunakan untuk penyempurnaan pelaksanaan kegiatan serupa di masa yang akan datang. Masyarakat sekitar Rawa Pening sangat mendukung dan antusias terhadap program restocking yang dilakukan dan merasakan manfaat positif dari kegiatan restocking meskipun belum signifikan. Dukungan, perencanaan, kontinuitas monitoring dan evaluasi menjadi unsur penting bagi keberhasilan program restocking. Saran yang diberikan setelahn evaluasi program pengabdian adalah perlunya tagging pada program restocking untuk memudahkan proses monitoring dan kontrol keberhasilan program restocking. Kata Kunci : Restocking, Pengabdian Masyarakat, Rawa pening The complexity of prevailing problems in Rawa Pening has resulted in decreasing fishery resources potential in particular affecting the population of local fish species. The extinction of endemic species is an immense loss as the ‘natural heritage’ will not be  passed on to the next generation, the reduced benefit of the species and the disturbed ecological balances due to the missing ecological niche. Community Service Team of FPIK Undip bears the responsibility to take important part in finding solutions to the problems encountered in Rawa Pening. One of the proposed recommendation is  Fish Restocking. This program was carried out for 5 months in 3 (three) stages. First, Ecological Review consisting socialization programs, training on restocking techniques, and location and environment surveys for determining the species to be restocked, the selected species is Red Tilapia (Oreochromis niloticus). Second, Pre-restocking: provision of fish fry for restocking and acclimatization. Third, Restocking, which is the core stage of this community  service activity: the release of 6,000 fish fry with the sizes of 5-10 cm  released in three locations which were determined during the pre-survey stage. The total fry was 2,000. Third, Monitoring: carried out to assess the programs achievement as well as to evaluate the activity implementation deficiencies which can be refered to in similar acitivies in the future. The neighboring community was very supportive and showed appreciation and enthusiasm to this restocking activity and has got valuable benefits to some extent although can not yet significantly measured. Supports, planning and continuous evaluation and monitoring will become the key to the restocking success. Recommendations after the evaluation include tagging in the restocking program to allow the program monitoring and control of the activity accomplishment. Key words : Restocking, Community Service, Rawa Pening
PENGARUH KOMPOSISI JENIS PAKAN YANG BERBEDA TERHADAP PERTUMBUHAN TUKIK PENYU SISIK (Eretmochelys imbricata) DI PENETASAN SEMI-ALAMI PENYU TAMAN NASIONAL KARIMUNJAWA (The Effect of Different Feeding Composition on Growth of Baby Hawksbills Turtle (Eretmochelys imbricata) in Semi-Natural Hatching Den in Karimunjawa National Park) Fadya Rachmi Puteri; Norma Afiati; Niniek Widyorini
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 14, No 2 (2019): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (279.544 KB) | DOI: 10.14710/ijfst.14.2.110-114

Abstract

Populasi alamiah penyu di Indonesia terus menurun 20 - 30% per tahun terutama lebih disebabkan oleh faktor manusia dibandingkan dengan faktor alam dan predator. Beberapa di antara teknik penyelamatan untuk pelestarian penyu antara lain melalui penetasan di sarang semi-alami, perlindungan translokasi habitat (konservasi in-situ), penegakan hukum, penyuluhan dan pemberdayaan masyarakat sekitar. Di sarang semi-alami jenis pakan yang tepat pada saat pemeliharaan awal tukik sebelum dilepas ke laut akan mempengaruhi kelulus-hidupan mereka. Padahal, sampai saat ini jenis pakan yang tepat untuk tukik belum banyak diketahui. Penelitian ini menggunakan tiga 3 perlakuan pakan (Sardinella lemuru 100%; Sargassum filipendula 100%; campuran S. lemuru dan S. filipendula 50:50%), analisis data enggunakan Rancangan Acak Lengkap (RAL) dengan 3 pengulangan. Secara statistik, diketahui bahwa tiap perlakuan pakan menghasilkan perbedaan pada variabel panjang, lebar karapas dan bobot tukik. Berdasarkan analisis alometri tukik E.imbricata selama penelitian, dihasilkan konstanta b < b antar perlakuan yang menunjukkan bahwa pertumbuhan bersifat alometri negatif pada variabel panjang karapas dan bobot tubuh serta panjang dan lebar karapas; kecuali pada hubungan panjang dan lebar karapas tukik dengan pakan S. lemuru. The population of sea turtles in Indonesia continued to decrease by 20-30% each year. The decline in natural turtle populations is caused mainly by human factors rather than natural factors and predator. Among others, rescue and preservation of turtles can be accomplished through rearing in a semi-natural den, protection of the habitat translocation (in-situ conservation), law enforcement, education and empowerment of local communities. The proper type of feed for the hatchlings to provide information on required nutrient in optimizing the growth has not been known until now. The methods used in this study was an experimental method apllying Complete Randomised Design with three feeding treatments (100% S. lemuru; 100% Sargassum filipendula; 50:50% mixed of S. lemuru and S. filipendula). The results showed that carapace length, carapace width and body weight differed statistically (P<0.05) in every feed treatment. Hatchlings showed negative allometric  (b < ) in carapace length to weight as also carapace length to its width. Isometry in carapace length and width is only shown by hatchlings fed with  S. lemuru
The Potential of Tiger Prawn Fry from Delta Mahakam, East Kalimantan Indonesia Bob Suroso; Johannes Hutabarat; Norma Afiati
International Journal of Science and Engineering Vol 6, No 1 (2014)
Publisher : Chemical Engineering Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4431.106 KB) | DOI: 10.12777/ijse.6.1.43-46

Abstract

Most of the life cycle of tiger prawns were estuarine (Delta Mahakam). In the juvenile stage life in estuarine and the adult stage in marine. The research objective is to assess the potential of the tiger prawn fry catches in the Mahakam Delta, as a source of tiger prawn fry in the Mahakam Delta area farms. Research using interviews and descriptive analysis through monitoring with fishermen. The results showed that the fry tiger prawn from the Delta Mahakam there on the beaches or the edge of the sea where the water is shallow and slightly brackish, as in the Delta Mahakam. Fry can be captured by using rumpon. Tiger prawns fry from Delta Mahakam durability is relatively higher than fry from the hatchery. However, the number and continuity of fry very limited because it depends on the season. Abundance of fry is determined by the number of larvae produced in the wild and their survival is greatly influenced by the availability of food. Doi: 10.12777/ijse.6.1.43-46 [How to cite this article: Suroso, B., Hutabarat, J., and Afiati, N. (2013). The Potential of Tiger Prawn Fry from Delta Mahakam, East Kalimantan Indonesia, International Journal of Science and Engineering, 6(1),43-46. Doi: 10.12777/ijse.6.1.43-46
PENGOLAHAN LIMBAH CAIR PUPUK KADAR AMONIAK TINGGI DENGAN PROSES GABUNGAN MICROALGAE DAN NITRIFIKASI-DENITRIFIKASI AUTOTROFIK Indro Sumantri; . Sumarno; Norma Afiati
Prosiding SNST Fakultas Teknik Vol 1, No 1 (2010): PROSIDING SEMINAR NASIONAL SAINS DAN TEKNOLOGI 1 2010
Publisher : Prosiding SNST Fakultas Teknik

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Proses biologis konvensional untuk penghilangan urea dan amonium pada air limbah pabrik urea menggunakan proses algae mikro atau proses nitrifikasi-denitrifikasi heterotrofik.  Proses yang menggunakan berbagai mikro algae mempunyai keunggulan input hara hanya senyawa P dan mampu menghilangkan urea secara total tetapi tidak mampu menghilangkan kandungan amoniumnya.  Proses nitrifikasi-denitrifikasi heterotrofik  membutuhkan input karbon organik yang tinggi pada proses denitrifikasinya sehingga biaya pengolahan menjadi tinggi. Tujuan penelitian dengan skala bangku ini untuk mengevaluasi kemampuan sistem gabungan proses algae mikro dan nitrifikasi-denitrifikasi autotrofik.  Algae mikro yang digunakan merupakan spesies algae yang tahan terhadap konsentrasi amonium tinggi dan mampu menghilangkan amonium selain urea.  Untuk proses nitrifikasi-denitrifikasi  menggunakan lumpur nitrifying yang bersifat autotrofik sebagai biokatalis.   Penyediaan lumpur nitrifying secara teknis sangat mudah.  Lumpur nitrifying berasal dari lumpur aktif yang diperoleh dari unit pengolahan limbah industri partikel board yang telah diaklitimasi pada kondisi konsentrasi amonium tinggi dan autotrofik. Keunggulan masing-masing proses tersebut bila digabung akan menghasilkan proses yang lebih efisien dan murah. Penelitian ini dilakukan dengan kondisi sebagai berikut : kadar SVI mikro algae 25 mL/L, kadar SVI lumpur 100 mL/L, laju aerasi yang digunakan 5 L/menit, waktu tinggal limbah 1 hari, rasio Ndan P : 20 : 1. Sedangkan sebagai variabel yang digunakan adalah beban amoniak antara 1000 – 3000 mg/L. Penurunan kadar amoniak yang diukur dilakukan pada akhir pengolahan yaitu setelah  bak lumpur. Hasil yang diperoleh menunjukkan bahwa prosentase penurunan kadar amoniak bisa mencapai 67 %. Kata kunci : proses mikroalga, proses nitrifikasi-denitrifikasi autotrofik, pengayaan dan breeding lumpur nitrifikasi  
ANALYSIS OF ENRICHMENT FACTORS AND CONTAMINATION FACTORS OF Cd, Cr AND Cu IN WESTERN FLOOD CANAL SEMARANG, CENTRAL JAVA Adam Putrarama Suyatno; Norma Afiati; Max Rudolf Muskananfola
Berkala Perikanan Terubuk Vol 49, No 2 (2021): Juli 2021
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/terubuk.49.2.1042-1052

Abstract

Pencemaran merupakan perubahan kualitas perairan menjadi buruk yang memiliki dampak negatifbagi biota perairan tersebut. Salah satu jenis cemaran di perairan yang berasal dari kegiatandomestik maupun industri adalah logam berat yang memiliki sifat toksik bagi biota perairan sepertimisalnya Cr, Cd dan Cu. Logam berat memiliki sifat mengendap sehingga diakumulasi dalamsedimen perairan yang menjadi habitat makrozoobentos. Makrozoobentos umumnya berfungsisebagai bioindikator yang peka karena cara hidupnya yang menetap di dasar perairan. Sungai BanjirKanal Barat mengalir melewati Kota Semarang, membawa berbagai macam limbah domestik danindustri. Oleh karena itu tujuan dari penelitian ini adalah mengetahui potensi cemaran 3 jenis logamberat berdasarkan faktor pengayaan dan faktor kontaminasinya. Penelitian ini dilaksanakan padabulan Januari 2021 menggunakan metode purposive sampling. Logam berat Cr, Cd dan Cudianalisis menggunakan alat Atomic Absorption Spectrophotometer (AAS). Hasil penelitianmenunjukkan kandungan logam kadmium pada air, sedimen dan bentos berkisar 0,019-0,078 mg/L;1,133-2,761 mg/kg dan 5,302-8,033 mg/kg. Kandungan logam krom pada air, sedimen dan bentospada rentang 0,460-1,481 mg/L; 36,137-49,918 mg/kg dan 53,178-54,177 mg/kg, sedangkankandungan logam tembaga pada air, sedimen dan bentos sebesar 1,070-1,339 mg/L; 0,052-0,063mg/kg dan 0,343-0,640 mg/kg. Hasil analisis data didapatkan faktor pengayaan logam kadmiumpada rentang 2,538-6,186. Sedangkan faktor pengayaan logam krom sebesar 0,269-0,372. Faktorpengayaan logam tembaga sebesar 0,0007-0,0009. Faktor kontaminasi logam kadmium didapatkanpada rentang 3,776-9,203. Faktor kontaminasi logam krom sebesar 0,415-0,803. Sedangkan faktorkontaminasi logam tembaga sebesar 0,0011-0,0014. Logam kadmium pada perairan tersebutterindikasi mengalami input antropogenik dan zat toksik yang tinggi, sedangkan logam krom dantembaga mengindikasikan pengayaan minimal dan tingkat kontaminasi rendah.
Meta-analysis of Indonesian Octopus laqueus Kaneko & Kubodera 2005 (Cephalopoda: Octopodidae) using Mt-DNA COI as Genetic Marker Nenik Kholilah; Norma Afiati; Subagiyo Subagiyo; Retno Hartati
Jurnal Kelautan Tropis Vol 24, No 1 (2021): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v24i1.10190

Abstract

O. laqueus was first discovered not long ago in 2005 in the Ryuku Islands, Japan. Its geographical distribution and molecular identification are therefore still rarely. Nucleotide sequences based on mt-DNA COI for O. laqueus that have been uploaded in the GenBank until before this study was carried out were only six sequences. Since DNA barcoding of mt-DNA COI has some advantageous characteristics, this study aimed to analyse the genetic difference of Indonesian O. laqueus to the data available in the GenBank. Samples were collected in 2019 - 2020 from Karimunjawa (n=16) and Bangka-Belitung (n=2). The mt-DNA COI was extracted using 10% chelex methods, PCR amplified using Folmer’s primer and sequenced in Sanger methods. Pairwise alignment and genetic distance were carried out in MEGA-X, whereas the phylogenetic tree was reconstructed using Bayesian methods. BLAST identification resulted in 685 bp with a range of 92,07-99,24  percentages of identity. The genetic mean pair-wise distances within-clade were 0,002 and 0,006, whilst the distance between the clade was 0.0883. Combining the suggestion with the ITF current, it is concluded that O. laqueus taken from Karimunjawa raised from the same species as those in Malaysia (MN711655) and Japan (AB302176). Specimens from Bangka-Belitung were suggested came from different species, as they were separated into the second clade by 8.83%. One single sample from Japan (AB430543) which laid outside the two clades by 11.63%-11.38% was also suggested to represent a different species. Overall, this study opens to various further studies on O. laqueus using other loci of genetic markers.
River Water Quality Based on Macrozoobentic Bioindicators in the Wonocolo Traditional Oil Mining Area Laily Agustina Rahmawati; Norma Afiati; Thomas Triadi Putranto
Jurnal Ilmu Lingkungan Vol 19, No 1 (2021): April 2021
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jil.19.1.29-35

Abstract

Many studies declared traditional oil mining in Wonocolo caused pollution, including river pollution.  During Covid-19 Pandemic, traditional oil mining in Wonocolo has been interrupted because world oil prices decreased. This made selling price of crude oil in Wonocolo declined. This made traditional oil mining decreased because oil wells were temporarily closed. The decrease in traditional oil mining might affect river water quality in Wonocolo. In a prior  study, the researcher had investigated water quality of Bungsu and Kragsaan River in Wonocolo, based on physicochemical parameters. The river had improved quality during Covid-19 Pandemic, seen from the decrease in the content of several chemical pollutants. Through this study, the researcher examined macrozoobentos community structure as a bio indicator of water quality, like assessing water quality of Bungsu and Kragsaan River based on biological indicators. This study used observation method by determining sample points purposively. Sample of macrozoobentos was analyzed using biodiversity index of Shannon-Wiener, species evennes index, and dominance index. Results of study showed Bungsu River had low biodiversity (H’ index 0.000 – 1.040), distressed community at B-1 and B-3 but stable at B-2, like high dominance at B-1 and B-3 but low at B-2. Kragsaan River also had low biodiversity (H’ index 0.000 – 1.010), unstable community at K-1 and K-3 like distressed at K-2, and low dominance at K-2 and K-3 but medium at K-1. Based on H’ index, Bungsu River was in the heavily polluted category at B-1 and B-3 and the medium polluted category at B-2. Meanwhile, Kragsaan River was in the heavily polluted category at K-1 and K-2 and the medium polluted category at K-3. This means although decreased levels of chemical pollutants at the sampling locations meant an increase in quality of water body, river ecosystem had not been able to rejuvenate condition during Covid-19 Pandemic.
Co-Authors . Sumarno Abdul Ghofar Adam Putrarama Suyatno Adriyani Samad Agus Sabdono Ahnan, Muhammad Firhan Maftuh Aji, Fajar Bayu Ali Djunaedi Anggieta, Yayank Dita Anhar Solichin Arif Rahman Arya Ksatria Fernanda Hendrawan Aryansyah, Akbar Atami, Sekar Putri Azis Nur Bambang Bambang Sulardiono Bob Suroso Chiesa, Francesco Te David Nugroho Diah Ayuningrum, Diah Dian Ayu Sapta Nur Utami Dicky Harwanto Djoko Suprapto Fadya Rachmi Puteri Fahmy Barik Farizan Adiya Pratama, Farizan Adiya Febrianto, Sigit Haeruddin Haeruddin Haque, Maharani Zahiratun Helfiana Tiuriska Perangin-angin Heni Susiati Indro Sumantri Indro Sumantri Irwani Irwani Iswanto, M. Fajar Fajar Johannes Hutabarat Khasani, Andro Kholilah, Nenik Lulu Adilla Latifah, Lulu Adilla Lusiana Rahayu Widiastuti Martha Wahyuningrum Mauritz L. Tobing Max Rudolf Muskananfola Max Rudolf Muskananfola Max Rudolf Muskananfola Megawati Arsita Putri Misbakul Munir Muhammad Arkan Zaky Rahman Mulkan Nuzapril Niniek Widyorini Novrizal Soni Riswandha, Novrizal Soni Nur Rochman Nurhuda, Izza Siti nurul latifah Ocky Karna Radjasa Oktavianto Eko Jati Prakoso, Teguh Budi Prijadi Soedarsono Pujiono Wahyu Purnomo Pujiono Wahyu Purnomo Pujiono Wahyu Purnomo, Pujiono Wahyu Purnami, Adelia Puspita, Like Viantika Jala Putra, Muchamad Iqbal Widiansyah Rachmawan, Dicky Setya Rahma, Dwi Aprilia Rahmawati, Laily Agustina Ramadhan, Faishal Retno Hartati Ria Purnama Dewi Sa’diyah, Halimatus Santi, Denita Irma Savitri Taurusiana Setiani, Heny Simangunsong, Erica Siti Rudiyanti Subagiyo Subagiyo Sumarno Sumarno Suradi Wijaya Saputra Surya Dwi Vrananta Sutrisno Anggoro Thomas Triadi Putranto, Thomas Triadi Tonny Bachtiar Ulfah Rismawati, Ulfah Yarianto Sugeng Budi Susilo Yuliana, Eka Yulfa