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All Journal Jurnal Keperawatan Florence Nightingale JAGO TOLIS : Jurnal Agrokompleks Tolis Jurnal Ilmiah AgriSains Sewagati: Jurnal Pengabdian Masyarakat Indonesia Indonesian Health Journal Jurnal Ekonomi Kreatif Indonesia International Journal of Sustainable Applied Sciences (IJSAS) Jurnal Agro Veteriner Jurnal Bioteknologi & Biosains Indonesia (JBBI) Indonesian Journal of Jamu Jurnal Bioteknologi & Biosains Indonesia
Magfira
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Environmental Science Health Professions Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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EFFECTIVENESS OF VARIOUS COMMERCIAL EXTENDERS IN CRYOPRESERVING SEMEN OF LOCAL INDONESIAN RAMS Setiadi, Dedi Rahmat; Magfira; Riwu, Ronny Markus Jami; Arifiantini, R Iis
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.9208

Abstract

Indonesian local sheep represent a particular species of sheep reared by farmers on small-scale farms. Semen freezing can be performed using either a homemade or commercial extender. The present study evaluated the effectiveness of several commercial extenders in freezing Indonesian local sheep. Semen was collected from three mature and healthy rams using an artificial vagina and evaluated. It was determined that only samples exhibiting sperm motility levels of greater than 70% would be included in the study. Subsequently, the semen from each ram was divided into three portions and diluted with Andromed, Sterydil, and OptiXcell, respectively. The frozen semen was then evaluated 24 hours post-freezing. The thawing process was conducted individually at 37°C for 30 seconds. The evaluation encompassed a range of metrics, including sperm motility, viability, and the presence of any abnormalities, in addition to the recovery rate. The results indicated that sperm frozen in Optixcell and Sterydil extenders exhibited superior motility, viability, and recovery rate than Andromed. The study also demonstrated no interaction between individual Ram and extender, and no differences were found between individuals. The study concluded that Optixcell and Sterydil extenders effectively froze Indonesian local sheep semen.
Co-Authors Ainun Nafisah Akbar Satria Bahari Andi Indriani Ibrahim Ariyani Goeliling Armelia, Vony Badariati Badariati Budiyanti, Ratu Reni Dedi Rahmat Setiadi Fahri, Mochamad Gagah Hendra Wijaya, Gagah Hendra Hasnidar IRA NURIYA SANTI Isma Suprapti Joni Tandi, Joni Kania Asri Liany Lakiu, Debora Rislianti Mardiyanti, Een Meindrawan, Bayu Moh Abubakar Talalu Munawarah Muztahidin, Nur Iman Niluh Puspita Dewi, Niluh Puspita Nurrofingah, Ulfa Pangesti, Raiza Tri Parmin R, Yana Novika R. Iis Arifiantini Ratu Reni Budiyanti Riwu, Ronny Markus Jami Rukiyah Nggili Shofa Aini, Fazriati Sukrang Sukrang, Sukrang Syafiyullah, Radian Vony Armelia