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All Journal HAYATI Journal of Biosciences Jurnal Hortikultura Indonesia (JHI) Microbiology Indonesia Media Penelitian dan Pengembangan Kesehatan Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati PROSIDING SEMINAR KIMIA BERITA BIOLOGI JURNAL BIOLOGI INDONESIA ZOO INDONESIA International Journal of Applied Biology Jurnal Fitofarmaka Indonesia ANNALES BOGORIENSES Journal of Tropical Crop Science Teknologi Indonesia Zoo Indonesia Makara Journal of Science Molekul: Jurnal Ilmiah Kimia Indonesian Journal of Jamu Annales Bogorienses Berita Biologi
ANDRIA AGUSTA
Research Center For Biology, Indonesia Institute Of Sciences (LIPI), Cibinong, Bogor 16911, Indonesia
Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Other

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Antioxidant Activity of Bioproduced Endophytic Fusarium Sp. HSFP-3 from Sijangkang Plant (Hornstedtia scyphifera Var. fusiformis) Koraag, Alfa Michael Gabriel; Farida, Yunahara; Tarigan, Elsera Br.; Efendy, Oscar; Agusta, Andria; Simanjuntak, Partomuan
Jurnal Fitofarmaka Indonesia Vol 11, No 3 (2024): JURNAL FITOFARMAKA INDONESIA (ENGLISH EDITION)
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jffi.v11i3.1252

Abstract

Endophytic mold Fusarium sp. HSFP-3 is one of the endophytic molds isolated from Sijangkang plants (Hornstedtia scyphyfera var. fusiformis). The purpose of this study is to conduct bioproduction, and test the antioxidant activity of bioproduced extracts and identify active fractions that have antioxidant activity. The research method started from the cultivation of endophytic mold HSFP-3 on PDB media for 21 days, the bioproduction results were extracted using ethyl acetate and acetone solvents. Antioxidant activity of HSFP-3 extract was determined by IC50 and AAI values, using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. Isolation of antioxidant active fraction was carried out by column chromatography method using sephadex LH-20 stationary phase and silica. Isolation of antioxidant active compounds of HSFP-3 extract using bioautography guided assay approach with TLC-DPPH Bioautography method. The antioxidant test results of HSFP-3 extract has moderate activity with IC50 value 74,64 and AAI 0,844. Fraction 2.4 is an active fraction that has spot compounds with higher yellow color intensity compared to other fractions. Spot with Rf 0.35 in fraction 2.4 has a dark zone under UV 254 light. has a sea blue color under UV 366 light and a dark purple color after with Vanillin-Sulfate reagent
Antioxidant and Antibacterial Activities of Plant and Endophytic Fungi Extracts from Syzygium myrtifolium Walp, with LC-HRMS Profiling of Active Extracts Efendi, Sintia Frisky; Suryati, Suryati; Rahmi, Dwinna; Praptiwi, Praptiwi; Ilyas, Muhammad; Agusta, Andria
Molekul Vol 20 No 2 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.2.16366

Abstract

ABSTRACT. The emergence of antibiotic resistance and oxidative stress-related diseases highlights the urgent need for novel bioactive compounds. This study investigates the potential of Syzygium myrtifolium Walp. and its endophytic fungi as sources of antibacterial and antioxidant agents. Sixteen endophytic fungi isolates were obtained from six plant parts and identified morphologically. Thin layer chromatography (TLC)-based chemical profiling demonstrated comparable secondary metabolite patterns between the plant and its endophytic fungi, indicating possible shared biosynthetic pathways. Antibacterial screening using TLC-bioautography and minimum inhibitory concentration (MIC) assays demonstrated that both the plant and endophytic fungi extracts inhibited the growth of Staphylococcus aureus and Escherichia coli, with Xylaria sp. showing notable activity. (SmDh4) exhibiting the strongest activity (MIC = 64 µg/mL). Antioxidant activity determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay indicated high to very high radical inhibition capacity, especially in SmRTd (AAI = 25.91) and SmAk1 (AAI = 24.97). Liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis unique secondary metabolites on endophytic fungi, including L-α-palmitin, α-eleostearic acid, and 8-methylnaphthalene-1,2-diol, which were detected exclusively in the endophytic fungi extracts, highlighting their potential as alternative antibacterial and antioxidant agents. Keywords: Antibacterial, antioxidant, endophytic fungi, secondary metabolites, Syzygium myrtifolium Walp.
Studi Akumulasi Pigmen β-Cryptoxanthin untuk Membentuk Warna Jingga Buah Jeruk di Daerah Tropika Sumiasih, Inanpi Hidayati; Arzam, Taruna Shafa; Poerwanto, Roedhy; Efendi, Darda; Agusta, Andria; Yuliani, Sri
Jurnal Hortikultura Indonesia (JHI) Vol. 9 No. 2 (2018): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (934.285 KB) | DOI: 10.29244/jhi.9.2.73-83

Abstract

ABSTRACTDegreening is a transformation process on peel which enables it to change color from green to orange on citrus fruits. The orange color of the peel comes from the mixture of carotenoid pigments, such as β-cryptoxanthin and β-citraurin. The pigments contributed in the formation of β-citraurin are β-cryptoxanthin and zeaxanthin. The objectives of this study were (1) to obtain proper degreening temperature in the orange color formation of several citrus varieties, and (2) to identify and determine pigments of β-cryptoxanthin pigment and total chlorophyll content in citrus peel after degreening. This study was conducted at PKHT IPB and LIPI Cibinong from July 2013 to December 2013, and from February 2016 to May 2017. About 100 ppm of ethylene gas was injected into a citrus-containing box using 5 ml syringe, then the box was placed in cool storage at 15 0C, 20 0C and room temperature, for 72 hours. The results showed that the best colors of Keprok Selayar and Keprok Tejakula were obtained by the degreening at 15 0C, in Siam Kintamani it was obtained by degreening at 20 0C. Degreening significantly reduced the total chlorophyll content, and increased β-cryptoxanthin content. The content of β-cryptoxanthin after degreening was 3 folds higher on highland Citrus reticulata than lowland citrus.Keywords: citrus color index, chlorophill, degreening, ethylene, tropical citrusABSTRAKDegreening adalah proses perombakan warna hijau pada kulit jeruk diikuti dengan proses pembentukan warna jingga. Warna jingga adalah campuran antara β-cryptoxanthin dengan β-citraurin. Pigmen yang berkontribusi dalam pembentukan β-citraurin adalah β-cryptoxanthin dan zeaxanthin. Tujuan penelitian ini ialah (1) Mendapatkan suhu degreening yang tepat dalam pembentukan warna jingga pada beberapa varietas jeruk, (2) Identifikasi dan penentuan kadar pigmen β-cryptoxanthin dan kandungan total klorofil pada kulit jeruk setelah degreening. Penelitian ini dilakukan di PKHT IPB dan LIPI Cibinong pada bulan Juli 2013 sampai Desember 2013, dan bulan Februari 2016 sampai Mei 2017. Degreening dilakukan dengan menginjeksikan gas etilen konsentrasi 100 ppm ke dalam wadah tertutup yang berisi jeruk menggunakan syringe 5 ml, kemudian disimpan pada suhu 15 0C, 20 0C dan suhu ruang, selama 72 jam. Hasil penelitian menunjukkan bahwa warna terbaik jeruk Keprok Selayar dan Tejakula diperoleh dengan degreening pada suhu 15 0C, Siam Kintamani diperoleh dengan degreening pada suhu 20 0C. Degreening dapat menurunkan kandungan total klorofil secara tajam, dan terbukti meningkatkan kandungan pigmen β-cryptoxanthin. Kandungan pigmen β-cryptoxanthin setelah degreening 3 kali lebih tinggi pada jeruk keprok dataran tinggi dibandingkan dengan dataran rendah.Kata kunci: citrus color index, degreening, etilen, jeruk tropika, klorofil
Potensi Antibakteri Ekstrak Daun Lasianthus Terhadap Bakteri Pseudomonas aeruginosa Purwantoro, R. Subekti; Siregar, Hartutiningsih-M.; Sudarmono, Sudarmono; Agusta, Andria
Jurnal Jamu Indonesia Vol. 1 No. 3 (2016): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v1i3.19

Abstract

Penelitian ini bertujuan untuk mengetahui sifat antibiotik dari ekstrak tumbuhan Lasianthus terhadap aktivitas bakteri Pseudomonas aeruginosa secara in-vitro. Ekstrak yang diuji adalah daun Lasianthus laevigatus, L. furcatus, dan L. obscurus. Ekstraksi tumbuhan dilakukan secara maserasi bertingkat dengan menggunakan ekstrak n-heksana, etil asetat, dan metanol. Potensi eskstrak sebagai antibakteri diuji melalui penghambatan aktivitas bakteri P. aeruginosa dengan menggunakan metode kertas cakram pada media agar Mueller-Hinton. Hasil penelitian menunjukkan bahwa ekstrak metanol pada daun L. furcatus memperlihatkan daya hambat tertinggi dengan zona hambat 12 mm (konsentrasi 50 μg), 19.5 mm (konsentrasi 100 μg), dan 20 mm (konsentrasi 200 μg), diikuti oleh L. obscurus dengan zona hambat 11 mm (konsentrasi 50 μg), 13 mm (konsentrasi 100 μg), dan 18.5 mm (konsentrasi 200 μg), sedangkan penggunaan ekstrak n-heksana dan etil asetat daya hambatnya rendah di bawah 10 mm, terkecuali ekstrak n-heksana pada daun L. obscurus daya hambat tinggi dengan zona hambat 11 mm (konsentrasi 50 μg).
Sensory Evaluation of the Quality of Kaffir Lime (Citrus hystrix DC.) Leaves Exposed to Different Postharvest Treatments Budiarto, Rahmat; Poerwanto, Roedhy; Santosa, Edi; Efedi, Darda; Agusta, Andria
Journal of Tropical Crop Science Vol. 8 No. 02 (2021): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.8.02.71-79

Abstract

This study aimed to evaluate the sensory attributes such as aroma, color and texture of kaffir lime leaves in response to various post-harvest treatment. The hedonic test approach was conducted by inviting 70 untrained panelists on seven post-harvest treatments, i.e. (D1) post-sortation fresh leaves, (D2) pre-sortation fresh leaves, (D3) cold-storage leaves, (D4) low temperature-storage leaves, (D5) brown dry leaves, (D6) fresh leaf-flour, and (D7) brown dry leaf-flour. The result showed that most of panelist agreed that aroma was the most important quality attribute that determined the level of preference in kaffir lime leaf products. Among seven tested products, the aroma, color, texture and overall impression of D1 was the most favorite one, while D4, D5, and D7 were assessed as low preference products. This work showed the importance of sortation and cold storage to maintain consumer likeliness. The criteria for sortation were green, clean, pest-disease free, scar free, high uniformity and fresh condition of leaves. Cold-storage at -20oC maintained the color and texture of kaffir lime leaves better than low temperature storage (5oC). To make leaf flour, the use of fresh green leaves was significantly better than brown dry ones. This finding might become the baseline data for the development of kaffir lime leaf product in the future.
The Effects of Preharvest Mild Shading on the Quality and Production of Essential Oil from Kaffir Lime Leaves (Citrus hystrix) Budiarto, Rahmat; Poerwanto, Roedhy; Santosa, Edi; Efendi, Darda; Agusta, Andria
Journal of Tropical Crop Science Vol. 9 No. 01 (2022): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.9.01.15-21

Abstract

Kaffir lime (Citrus hystrix DC) is a less popular citrus species commonly used as a food spice and a source of essential oil. Early studies report the success of preharvest mild shading to increase leaf yield, although there is still limited information on the effect of preharvest shading on the quality of essential oil produced. The aim of this current study is to evaluate the effect of preharvest mild shading factors on the yield, physical characteristics, and metabolite fingerprinting of kaffir lime leaves essential oil (KLLEO). One-year-old kaffir lime trees were sampled in two preharvest treatments, i.e., open sun and mild shading (24% light reduction) at Pasir Kuda experimental field, Bogor, Indonesia. Statistical analysis showed that there was no significant effect of preharvest treatment on yield and physical characteristics (color, specific gravity, and refractive index) of KLLEO. In contrast, there was a metabolite fingerprinting variation of KLLEO as an effect of mild shading. The relative percentage of bergamol, citronellol, caryophyllene oxide, citronellic acid, isopulegol, isopulegyl formate, limonene, linalool, and linalool oxide was increased by mild shading. On the other hand, the main metabolite (citronellal) was significantly reduced by about 10% in shading treatment, as compared to the open-sun ones.
Yield and Physicochemical Characteristics of Kaffir Lime Leaf Essential Oils Subjected to Different Post-Harvest Treatment Budiarto, Rahmat; Poerwanto, Roedhy; Santosa, Edi; Efendi, Darda; Agusta, Andria; Rofiq, Muhamad Abdul
Journal of Tropical Crop Science Vol. 11 No. 02 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.02.97-104

Abstract

The importance of kaffir lime leaf as essential oils (EOs) raw material is starting to get attention because of its commercial value; however, there is no quality reference for kaffir lime leaf EOs, especially in response to various post-harvest handlings. This study aimed to describe the physicochemical characteristics and yield of kaffir lime EOs subjected to different post-harvest. Bogor originated-kaffir lime leaf was prepared to be subjected to several post-harvest treatments, i.e., control/fresh green leaves (P1); milling to produce green leaf flour (P2); drying to produce dry brown leaf (P3), and milling and drying to produce brown leaf flour (P4). The result showed that post-harvest treatment generally decreases an oil yield and increases darkness color, specific gravity and refractive index of tested EOs. Additionally, post-harvest treatment also changes metabolite profile revealed by GCMS analysis. The relative percentage of caryophyllene and citronellol tends to increase, while the linalool and citronellal levels decrease due to tested post-harvest treatment. It was implied that for the benefit of the fragrance industry with a high citronellal requirement, EOs should be made from fresh green leaves and the leaves should be avoided from drying and powdering treatment.
Chemical Compositions and Antibacterial Effect of Essential Oil of Key Lime Leaves (Citrus aurantifolia Swingle: Rutaceae) Jamal, Yuliasri; Pariwidjayanti, Aris Medta; Agusta, Andria
Annales Bogorienses Vol. 16 No. 2 (2012): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Steam distillation of essential oil from fresh key leaves of Key lime (Citrus aurantifolia Swingle) gave 0.4% yellow essential oil with distinctive fragrance. Five out of 40 components of the essential oil were identified as the major components with content above 5%, they were geranial (103%). limonene (10.2%), neral (8.94%), caryphyllene (5.72%), and citronellal (5.41%). The essential oil was active against four bacterial test, Bacillus subtilis NBRC 3134, Staphylococcus aureus NBRC 14276, Micrococcus luteus NBRC 14218, and Escherichia coli NBRC 14237. B. subtilis was the most sensitive bacterium with the widest inhibition area at 50% concentration of essential oil. Minimum Inhibition Concentration (MIC) value of essential oil against B. subtilis was 0.125%.
BIODIVERSITY AND ANTIBACTERIAL ACTIVITIES OF ENDOPHYTIC FUNGI ASSOCIATED WITH UNCARIA GAMBIER (HUNTER) ROXB. VAR. UDANG FROM JASINGA, BOGOR, INDONESIA Palupi, Kartika Dyah; Kamal, Andi Saptaji; Marlina, Lina; Hafid, Lukman; Evana; Tarigan, Elsera Br; Raunsai, Marlin Megalestin; Ilyas, Muhammad; Kanti, Atit; Agusta, Andria
Berita Biologi Vol 22 No 2 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.713

Abstract

Endophytic fungi living in medicinal plant tissues are potential bioactive metabolite sources for drug discovery and development. However, the research regarding endophytic fungi associated with Indonesian medicinal plants is still limited. This study aimed to explore the biodiversity of endophytic fungi associated with the medicinal plant Uncaria gambir (Hunter) Roxb. var. udang and their antibacterial activities. Twenty-seven endophytic filamentous fungi were isolated from the surface-sterilized leaves, stems, and roots of Uncaria gambir (Hunter) Roxb. var. udang collected in Jasinga, Bogor, West Java, Indonesia. The observation based on the morphological characters both macroscopically and microscopically, as well as chemical profiling using thin layer chromatography (TLC), revealed that some of the isolated endophytic fungi were identical. Overall, the isolated endophytic fungi consist of six Coelomycetes, three Dematiaceae, four Pestalotiopsis sp., two Penicillium sp., one Aspergillus terreus, and two fungi were unidentified. Antibacterial activities of the ethyl acetate extract from the isolated endophytic fungi associated with Uncaria gambir (Hunter) Roxb. var. udang were observed against E. coli, B. subtilis, S. aureus, and M. luteus. Twelve types of isolated endophytic fungi from Uncaria gambir (Hunter) Roxb. var. udang exhibited antibacterial activities.
ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF ETHYL ACETATE EXTRACT OF ACTINOMYCETES ISOLATED FROM TERMITE NESTS Fathoni, Ahmad; Kamal, Andi Saptaji; Hafid, Lukman; Marlina, Lina; Efendy, Oscar; Putri, Ade Lia; Praptiwi, Praptiwi; Agusta, Andria
Berita Biologi Vol 23 No 1 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2024.3618

Abstract

Actinomycetes are an important source for the discovery of bioactive secondary metabolite compounds. Over 10,000 bioactive metabolite compounds have been isolated from terrestrial actinomycetes with various biological activities, such as antibiotic, antiviral, anti-inflammatory, antitumor, anticancer, and antioxidant. One source of origin for actinomycetes is soil, including termite nests. Utilization of natural sources such as actinomycetes from termite nests as antioxidants and antibacterials can be an alternative source of production of new secondary metabolite compounds. This research aims to evaluate the antioxidant and antibacterial activities of actinomycete extracts from termite nests. Antioxidant activity using the DPPH free radical scavenging method, and antibacterial activity against S. aureus. Antioxidant and antibacterial activity were performed by using the TLC dot-blot, TLC bioautography, and microdilution methods to determine the inhibitory concentration of 50% (IC50), antioxidant activity index (AAI), and minimum inhibitory concentration (MIC). From the 33 extracts tested, 16 extracts showed antioxidant activity with IC50 values range of 76.64-126.22 µg/mL or AAI value > 0.05 (moderate), and 8 extracts had moderate antibacterial activity against S. aureus (MIC values range of 256-512 µg/mL). Future research for scaling-up of actinomycete culture, isolating of active compounds, determining of the chemical structure of active compounds, and further testing as antioxidants and antibacterials still need to be carried out.
Co-Authors A.A. Ketut Agung Cahyawan W Agus Budiawan Naro Putra AHMAD FATHONI Ahmad Fathoni Ahmad Fathoni Andi Saptaji Kamal Anita, Syahfitri Antonius Herry Cahyana Antonius Herry Cahyana ARIEF NURKANTO Arif Nurkanto Arif Nurkanto Arif Nurkanto Atit Kanti Chairul Chairul Chairul Chairul DEWI WULANSARI Dewi Wulansari Dwinna Rahmi Edi Santosa Efendi, Darda Efendi, Sintia Frisky Efendy, Oscar Efendy, Oscar Effendi, Oscar Elsera Br Tarigan, Elsera Br Evana Farida, Yunahara Hafid, Lukman Hartutiningsih-M. Siregar HEDDY JULISTIONO Heddy Julistiono Herjuno Ari Nugroho, Herjuno Ari Inanpi Hidayati Sumiasih, Inanpi Hidayati Kamal, Andi Saptaji Kartika D. Palupi Kartika Dyah Palupi Kartika Dyah Palupi Kartika Dyah Palupi Keim, Ary P Koraag, Alfa Michael Gabriel Lestari, Vera Budi lina marlina, lina Listyaningsih, Febrianti Listyaningsih, Febrianti Marlin Megalestin Raunsai Mohammad Fathi Royyani, Mohammad Fathi Muhamad Ilyas Muhamad Ilyas, Muhamad Muhammad Ilyas Muhammad Ilyas N.L.P. Rischa Phadmacanty Octavia Siti Nurhayati Palupi, Kartika D. Pariwidjayanti, Aris Medta Pariwidjayanti, Aris Medta Partomuan Simanjuntak Phadmacanty, N.L.P. Rischa Pipit Irawati Praptiwi Praptiwi Praptiwi Praptiwi Praptiwi Praptiwi Praptiwi Praptiwi, Praptiwi, - Putra, Aldho Pramana Putri, Ade Lia R. Subekti Purwantoro Rahmat Budiarto Rahmawati Ridwan Raunsai, Marlin Megalestin Roedhy Poerwanto Rofiq, Muhamad Abdul Royani, M Fathi S. Achmadi, Suminar S. Achmadi, Suminar Siti Aisyah Sri Yuliani SUDARMONO SUDARMONO Suryati Suryati Tarigan, Elsera Br. Taruna Shafa Arzam, Taruna Shafa Tiwi, Praptiwi Wartika Rosa Farida Wellyzar Sjamsuridzal YULIASRI JAMAL Yuliasri Jamal