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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Hortikultura Indonesia (JHI) Vegetalika Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmiah Aplikasi Isotop Radiasi Jurnal Hortikultura AGRIVITA, Journal of Agricultural Science Jurnal Agro Jurnal AGROSAINS dan TEKNOLOGI Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biologi Tropis Journal of Tropical Crop Science AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Molekul: Jurnal Ilmiah Kimia Menara Perkebunan Jurnal Farmamedika (Pharmamedica Journal) Akta Agrosia Agrotech Journal

Syarifah Iis Aisyah

Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian BogorJl. Meranti, Kampus IPB Darmaga, Bogor, 16680, Indonesia

Author-ID : 223003

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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MULTIPLIKASI IN VITRO ANGGREK HITAM (Coelogyne pandurata Lindl.) PADA PERLAKUAN KOMBINASI NAA DAN BAP Kartiman, Roni; Sukma, Dewi; Aisyah, Syarifah Iis; Purwito, Agus
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 1 (2018): June 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

In Vitro Multiplication ofÃ‚Â Black Orchid (Coelogyne pandurata Lindl.)Ã‚Â Using the Combination of NAA and BAPABSTRACTBlack orchid is an indigenous plant from Kalimantan, Indonesia. It becomes endangered because of forest over-exploitation and its low natural reproduction rate. Tissue culture is considered to offer a solution to conserve and propagate this species. The aim of this research was to evaluate the effect of Naphtalene Acetic Acid (NAA) and 6-Benzile Amino Purine (BAP) on shoots multiplication of black orchid. The basic medium used was a half of Murashige & Skoog (MS) composition supplemented with 150 mLL-1 coconut water. Initial explants used were 6-month-old shoots of germinating seeds. The shoot cultures were incubated for 23 weeks. Results showed that the best combination for shoot multiplication was NAA 0.0 mgL-1 with BAP 0.2 mgL-1. Shoot grew better on medium with BAP and without NAA while roots growth was better on medium without the two plant growth regulators. The addition of BAP up to 0.3 mgL-1 increased the leaf number, which however decreased at higher BAP concentration.Keywords: BAP, black orchid, Coelogyne pandurata, multiplication, NAAÃ‚Â ABSTRAKAnggrek hitam merupakan flora langka asli Kalimantan, Indonesia. Keberadaa anggrek ini di alam semakin langka akibat eksploitasi berlebihan dan sulitnya perbanyakan secara alami. Kultur jaringan merupakan metode untuk mengatasi kelangkaan anggrek ini. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi NAA dan BAP terhadap multiplikasi anggrek hitam. Media dasar yang digunakan adalah Ã‚Â½ MS dengan penambahan air kelapa 150 mLL-1. Eksplan yang digunakan adalah tunas hasil semai biji umur 6 bulan. Kultur tunas diinkubasi selama 23 minggu. Hasil penelitian menunjukkan bahwa kombinasi terbaik untuk multiplikasi tunas adalah NAA 0 mgL-1 dengan BAP 0,2 mgL-1. Tunas tumbuh lebih baik dalam media dengan penambahan BAP tanpa NAA, sedangkan akar pada media tanpa NAA dan BAP. Penambahan BAP sampai 0.3 mgL-1 mampu meningkatkan jumlah daun, namun menurun dengan penambahan di atas konsentrasi tersebut.Kata Kunci: anggrek hitam, BAP, Coelogyne pandurata, multiplikasi, NAA

Pembentukan Populasi Dasar Krisan (Dendranthema grandiflora Tzelev) untuk Materi Induksi Mutasi Kimia [Population Formation of Chrysanthemum (Dendranthema grandiflora Tzelev) as Materials for Induced Mutation using Chemical Mutagen] Suryawati, nFN; Aisyah, Syarifah Iis; Marwoto, Budi; Kurniati, Ridho
Jurnal Hortikultura Vol 31, No 2 (2021): Desember 2021
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v31n2.2021.p137-146

Induksi mutasi dapat dilakukan pada beberapa materi tanaman. Jenis materi tanaman menentukan keberhasilan terbentuknya mutan. Keberhasilan in vitro mutagenesis dipengaruhi oleh stadia tumbuh bahan tanam yang digunakan selama peroses mutagenesis. Mutagenesis dalam bentuk kalus berpotensi mendapatkan mutan solid. Penelitian ini bertujuan mendapatkan kalus sebagai materi untuk induksi mutasi kimia serta media produksi kalus terbaik secara in vitro. Bahan yang digunakan yaitu daun plantlet krisan varietas Reagent Pink dan Jaguar Pink. Penelitian menggunakan Rancangan Acak Lengkap (RAL) terdiri atas tujuh kombinasi perlakuan media dasar MS dengan penambahan beberapa zat pengatur tumbuh. Kalus krisan kedua varietas (Reagent Pink dan Jaguar Pink) dapat terbentuk pada semua kompisisi media. Struktur kalus remah, dengan warna bervariasi hijau kekuning-kuningan hingga cokelat. Ukuran dan bobot segar kalus tidak menunjukkan adanya perbedaan. Media M7 merupakan media yang disarankan untuk produksi kalus sebagai media dasar induksi kalus.KeywordsInduksi mutasi; In vitro; Kalus; KrisanAbstractMutagen induced mutation in many explants and plant materials. These materials were determined mutant. The success of in vitro mutagenesis is influenced by the growing stadia of planting materials used during the mutagenesis process. Mutagenesis in the form of calluses has the potential to obtain solid mutants. The objectives of these studies were to find out materials explant for chemical induce mutation and in vitro media for callus production. Reagent Pink and Jaguar Pink leaf were used as materials. The experiment design was Complete Random Design, with seven treatment of MS (Murashige and Skoog) base medium and the addition of some growing regulatory subtances. Both of chrysanthemum varieties (Reagent Pink and Jaguar Pink) produced calli in all of tested media. The friable calli were obtained. There were same variation color of callus, e.g green, yellowish, and brownish. The variation of size and callus weight were also obtained eventhough it were not significantly different. M7 medium was recommended media for callus production, then it were used for induce mutation materials as based population.

Induksi Mutasi pada Stek Pucuk Anyelir (Dianthus caryophyllus Linn.) melalui Iradiasi Sinar Gamma Syarifah Iis Aisyah; Hajrial Aswidinnoor; Asep Saefuddin; Budi Marwoto; Sarsidi Sastrosumarjo
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 1 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

It has been a common knowledge that LD50 is commonly used in estimating optimal doses of gamma irradiation in a breeding program. This research was aimed at observing radiosensitivity of five carnation's genotypes to gamma irradiation, to find the LD50 of carnation's cuttings, and to obtain solid mutants from five numbers of Carnation. For cuttings, carnation genotype number 10.8 was the most insensitive to gamma rays, whereas number 24.15 was the most sensitive one. LD50 of carnation's cuttings were obtained around 49 -72 gray. There were 19 mutants produced from this treatment. The desired mutans were mostly produced from the treated 24.1 genotype whereas the character mutans were mostly observed in MV2 generation. Irradiation treatment on genotype 24.1 produced most stabile mutans while the less was in genotype 24.14. The produced mutants were qualitatively different in colour and petal shape, and stabile till third generation. Key words: LD50, gamma irradiation, induced mutation, carnation.

Ketahanan 25 Genotipe Tomat (Solanum lycopersicum Mill.) terhadap Pecah Buah dan Korelasinya dengan Karakter-karakter Lain Sri Wahyuni; Rahmi Yunianti; Muhamad Syukur; Joko Ridho Witono; Syarifah Iis Aisyah
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 3 (2014): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTFruit cracking is one of the major constraints in tomato production in lowland areas. This reduces fruit quality and quantity both as freshmarket and processed fruits. The objective of this study was to determine the resistance of 25 tomato genotypes to fruit cracking, correlation to others characters and their genetic similarity. The experiment was conducted from April until August 2012 at Leuwikopo Field Station, Bogor Agricultural University. A randomized complete block design was used with three replications. Fruit crack index was used to evaluate tomato fruit crack resistance. Correlation and impacts of variables were analyzed by path analysis. The genetic similarity was analyzed by clustering analysis. Result of resistance evaluation showed that fruit crack index of 25 tomato genotypes ranged from 0.00 to 20.58. Fruit crack index was directly related to percentage of fruit crack weight per plant, percentage of number of fruit crack per plant and total soluble solids. Cluster analysis showed that the genotypes could be clustered into four groups.Keywords: clustering analysis, genetic diversity, path analysis

Pendugaan Daya Gabung dan Heritabilitas Komponen Hasil Tomat pada Persilangan Dialel Penuh Helfi Eka Saputra; Muhamad Syukur; Syarifah Iis Aisyah
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 3 (2014): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACT Hybridization is one of plant breeding techniques to obtain high yielding varieties. Combining ability and heritability information are needed for evaluating hybrids’ performance. The objective of this research was to estimate the combining ability and heritability of tomato yield component traits in a full dialel crosses at lowland. The experiment was conducted at Leuwikopo Experimental Station IPB, Darmaga, Bogor from March until August 2013. The random model of Griffing method I was used to estimate general combining ability, specific combining ability and heritability. The experiment used a randomized complete block design (RCBD) with three replications. IPBT1, IPBT13 and IPBT84 had a good general combining ability (GCA) for weight per fruit, fruit size and weight of fruit per plant. IPBT30 and IPBT33 had a good general combining ability (GCA) for number of fruits per plant. IPBT13 × IPBT1 hybrid showed a high and positive specific combining ability (SCA) values for all characters, therefore it can be used for hybrid variety. The highest broad and narrow sense heritability was obtained for fruit size, meanwhile the lowest was obtained for weight of fruit per plant. Weight per fruit, fruit size and weight of fruit per plant characters were influenced by additive and dominant gene effects, whereas number of fruit per plant was influenced by additive gene effects. Keywords: additive, dominant, GCA, SCA, heritability

Sensitivitas dan Keragaan Tanaman Coleus sp. terhadap Mutasi Induksi Kimia Menggunakan Ethyl Methane Sulfonate (EMS) Aplikasi Cara Rendam dan Tetes Dia Novita Sari; Syarifah Iis Aisyah; dan Muhammad Rizal Martua Damanik
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 1 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Variant of Coleus sp. in Indonesia is very low. Induced mutation by Ethyl Methane Sulphonate (EMS) is able to increase variant of Coleus sp.. EMS is a mutagen that is effective and efficient in causing mutations. In mutation breeding, the high variant usually occurs around LC50 (Lethal Concentration). The objective of this study were (1) to obtain the LC50 value and sensivity of coleus plants, and (2) to evaluate the response of plant growth and to obtain new variance of coleus used soak and drops by EMS application. The experiment was arranged in randomized complete block design with three replications in each coleus. The materials used two Coleus sp. shoot cuttings i.e., C. amboinicus Lour. and C. blumei the purple/green color and the red. The concentration of EMS consisted of 0.00, 0.50, 0.75, 1.00, and 1.25% with application soak method (100 minutes) and drops (3 drops pipette). The results showed that the LC50 value of C. amboinicus Lour. 5.86% (soak). The LC50 value of C. blumei the purple/green color was 0.69% (soak), 0.82% (drops) and the red color 0.29% (drops), 0.89% (drops). C. blumei (the purple/green and red) was higher sensitivity rather than C. amboinicus Lour. There was an interaction between treatments on C. amboinicus Lour. whereas C. blumei (purple/green and red) had no interaction among treatments. C. amboinicus Lour. produced one putative mutant and C. blumei purple/green produced four putative mutants based on the morphology.Keywords: Coleus amboinicus Lour., Coleus blumei, LC50, putative mutan

Induksi Mutasi Tanaman Leunca (Solanum nigrum L.) untuk Meningkatkan Keragaman Kandungan Tanin Siti Hartati Yusida Saragih; Syarifah Iis Aisyah; dan Sobir
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 47 No. 1 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Leunca (Solanum nigrum L. ) is one of the indigenous vegetables in Indonesia. The fruit of this vegetable is green and has bitter taste because of its tannin content. The objectives of this study were to get lethal dose 50 (LD50), diversity and to obtain candidates of mutant plants with high and low tannin content in young fruit and ripe fruit in leunca. The research was conducted at Pasir Kuda experimental station, Bogor; National Nuclear Energy Agency (BATAN), Jakarta; and postharvest laboratory of the Center for Tropical Horticulture Studies (PKHT IPB), Bogor, from December 2016 to July 2017. Leunca accession of SN 20 and SN 29 were irradiated using doses of 0, 100, 150, 200, and 250 Gy of gamma ray. The LD50 values for SN 20 and SN 29 were 171.944 Gy and 190.949 Gy, respectively. The analysis of tannin content was performed based on permanganate method by titration using potassium permanganate. The results showed that the highest tannin content of green stage fruit was found in SN20D3.5 with 200 Gy dose and the lowest was found in SN29D1.5 with 100 Gy dose. The highest tannin content of ripe fruit was found in SN20D4.6 with dose of 250 Gy and the lowest was found in SN20D3.1 with dose of 200 Gy. Recommended tannin content for leunca is the low tannin content.Keywords: diversity, gamma ray, indigenous vegetable, irradiation, LD50

Evaluasi Metabolomik Mutan Putatif Bawang Putih (MV3) Hasil Iradiasi Gamma LD50 Utin Winarni; Diny Dinarti; Syarifah Iis Aisyah
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 50 No. 1 (2022): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Peningkatan keragaman genetik bawang putih dapat melalui pemuliaan mutasi buatan menggunakan sinar gamma. Penelitian bertujuan untuk mempelajari pengaruh dosis iradiasi gamma pada LD50 terhadap pertumbuhan, produksi, dan kandungan senyawa metabolit generasi MV3 tiga genotipe bawang putih. Penelitian dilaksanakan pada bulan Mei sampai September 2020 di Kebun Percobaan Pasir Sarongge IPB. Percobaan terdiri atas enam populasi mutan bawang putih tanpa ulangan, yaitu Ciwidey 0 Gy , Ciwidey 6 Gy, Tawangmangu Baru 0 Gy, Tawangmangu Baru 8 Gy, Lumbu Kuning 0 Gy, Lumbu Kuning 10 Gy. Setiap populasi memiliki jumlah individu yang berbeda tergantung ketersediaan bahan tanam dari generasi sebelumnya. Total 364 tanaman yang diuji tersebut diamati pertumbuhan dan produksi tanaman. Hasil penelitian paparan dosis LD50 pada semua genotipe bawang putih menurunkan diameter umbi, jumlah siung dibandingkan kontrol. Senyawa baru yang terdeteksi pada CW 6 Gy yaitu 2-Hexanol; 2-Hexanol, 3,4-dimethyl-; Cyclopentene, 1,2,3,3,4-pentamethyl-; Trisulfide, methyl 2-propenyl. Senyawa baru yang terdeteksi pada LK 10 Gy yaitu Butyl isobutyl phthalate; 2-Pentanol, 2-methyl-; Ether, 2-chloro-1-methylethyl isopropyl; Hentriacontane; Isoamyl lactate. Senyawa baru yang terdeteksi pada TMB 8 Gy yaitu; Cyclopentene, 1,2,3,3,4-pentamethyl-; Decane; 3-Vinyl-1,2-dithiacyclohex-4-ene; Diallyl disulphide. Kata kunci: allicin, antimikroba, GC-MS, metabolomik, mutan

Pengelolaan Pemupukan Kelapa Sawit (Elaeis guineensis Jacq.) di Kebun Tanjung JatiPengelolaan Pemupukan Kelapa Sawit (Elaeis guineensis Jacq.) di Kebun Tanjung Jati Monica Christina Natalia; Syarifah Iis Aisyah; . supijatno
Buletin Agrohorti Vol. 4 No. 2 (2016): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Kegiatan magang ini dilaksanakan pada 10 Februari hingga 9 Juni 2014 di Kebun Tanjung Jati. Kegiatan magang bertujuan untuk meningkatkan kemampuan mahasiswa dalam memahami proses kerja secara nyata di lapangan, serta dapat meningkatkan pemahaman dan keterampilan manajemen dalam pengelolaan tanaman kelapa sawit. Pemupukan kelapa sawit yang efektif dan efisien harus memenuhi prinsip 5T (Tepat Dosis, Tepat Waktu, Tepat Cara, Tepat Tempat, dan Tepat Jenis). Pengamatan yang dilakukan terhadap ketepatan jenis dan cara yang diaplikasikan sudah tepat namun ketepatan dosis pemupukan serta ketepatan waktu masih perlu ditingkatkan

Induksi Mutasi Fisik pada Paku Bintik (Microsorum punctatum) melalui Iradiasi Sinar Gamma Qisthi Kustia Rahman; Syarifah Iis Aisyah
Buletin Agrohorti Vol. 6 No. 3 (2018): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Pemuliaan mutasi dengan iradiasi sinar gamma merupakan salah satu cara dalam meningkatkan keragaman genetik tanaman. Tujuan penelitian ini adalah mengetahui radiosensitivitas dan nilai LD50 (Lethal dose 50) sinar gamma pada M. punctatum serta mengamati keragaan tanaman M. punctatum hasil iradiasi sinar gamma pada MV1. Nilai LD50 merupakan salah satu parameter untuk mengukur tingkat sensitivitas suatu jaringan terhadap iradiasi. Semakin rendah nilai LD50 suatu jaringan tanaman, semakin tinggi tingkat radiosensitivitasnya, sehingga semakin besar peluang terjadinya mutasi. Penelitian ini menggunakan rancangan acak lengkap (RAL) faktor tunggal (dosis iradiasi sinar gamma) dengan 3 ulangan. Iradiasi sinar gamma diberikan terhadap stek rimpang dengan satu daun dari tanaman M. punctatum secara tunggal (acute irradiation) menggunakan Gamma Cell 220 dengan dosis 0, 10, 20, 30, 40, dan 50 gray. Semua rimpang dengan satu daun hasil iradiasi ditanam di lapangan hingga 16 minggu setelah iradiasi (MSI). Hasil penelitian menunjukkan bahwa pemberian iradiasi sinar gamma pada M. punctatum menghasilkan LD50 sebesar 86,25 gray dan perlakuan iradiasi sinar gamma antar perlakuan berbeda nyata terhadap tinggi dan lebar daun, sedangkan karakter jumlah daun tidak berbeda nyata. Perlakuan iradiasi juga menyebabkan defisiensi warna hijau pada daun M. punctatum. Secara morfologi, perlakuan iradiasi sinar gamma terhadap M. punctatum belum menghasilkan mutan-mutan yang diinginkan, namun secara genetik, terdapat penggandaan jumlah kromosom dan aberasi kromosom pada perlakuan dosis iradiasi tertinggi (50 gray).

Co-Authors , Krisantini Abdul Hakim Agus Purwito Arum Sholikhah ASEP SAEFUDDIN Awang Maharijaya Aziz, Sandra A Azri Kusuma Dewi Azzahra, Elmi Irmayanti Bagas Akmala Putra Budi Marwoto Dewa Ngurah Suprapta Dewi Sukma Dia Novita Sari Diny Dinarti Endang Gati Lestari Eny Rolenti Togatorop Fahmi Muhammad Cokrosudibyo Hafizah, Rumaisha Afifatul HAJRIAL ASWIDINNOOR Hasna Dama Hayati, Dhieni Helfi Eka Saputra Helfi Eka Saputra Helfi Eka Saputra Ika Roostika Joko Ridho Witono Kartiman, Roni Kartiman, Roni Kunto Wibisono Kunto Wibisono Kunto Wibisono Kurniati, Ridho Latifah K Darusman Mariana Susilowati Marthin, Yodi Marwoto, Budi Masna Maya SINTA Monica Christina Natalia Muh. Nur Alif Rapi Muhamad Rizal Martua Damanik Muhamad Syukur Muhammad Ridha Alfarabi Istiqlal Muhammad Riyo Agung Kurnia Muhammad Syukur Mulyana, Erik Muthi'ah Khairun Nisa Nabilah, Noorfakhriyah Ahsanti Natalia, Monica Christina Nella Angelina Simanjuntak Ni Made Armini Wiendi Niken Kendarini Nurul Komala Qisthi Kustia Rahman RAGAPADMI PURNAMANINGSIH Rahman, Qisthi Kustia Rahmi Yunianti Rara Annisaur Rosyidah Ritonga, Arya Widura Riyan Alifbi Putera Irsal Rohim Firdaus Roni Kartiman Roni Kartiman Roni Kartiman Rosa Yunita Rusmiyati, Henny S Sudarsono Sarsidi Sastrosumarjo Sembiring, Rinawati Setiawan, Farensa Ikman Dedi Siti Hartati Yusida Saragih Sobir Sobir Sobir Sobir Sobir Sobir Sobir Soeranto Human Sri Suhesti Sri Suhesti Sri Suhesti Sri Wahyuni Sukma, Dewi Sulassih, . Supijatno Suryawati, nFN Tamami, Djoko Theresia Dame Angel Lie Manurung Trikoesoemaningtyas Utin Winarni Waras Nurcholis Wijaya Murti Indriatama Wijaya Murti Indriatama Wulan Septiningtyas Kurniajati yahidah Rosyidah Anshori Yasmin, Zahra Fadhlia Yoshua Shandy Yudha Yukarie Ayu Wulandari Yukarie Ayu Wulandari Yustia Yulianti Zahra Fadhlia Yasmin

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